FlyBase Allele Report: Dmel\Su(var)205[5]

General Information

Symbol

Dmel\Su(var)205⁵

Species

D. melanogaster

Name

FlyBase ID

FBal0016507

Feature type

allele

Associated gene

Dmel\Su(var)205

Associated Insertion(s)

Carried in Construct

Also Known As

Su(var)2-5⁰⁵, Su(var)205⁰⁵, Su(var)2-5⁵, Hp1⁵

Key Links

Genomic Maps

JBrowse

Allele class

loss of function allele

Mutagen

X ray

Nature of the Allele

Allele class

loss of function allele

(Seum et al., 2000, Kellum and Alberts, 1995)

Mutagen

X ray

(Eissenberg and Hartnett, 1993, Eissenberg et al., 1992)

Progenitor genotype

Cytology

Description

Frameshift after ten amino acids.

(Kellum and Alberts, 1995)

Dinucleotide deletion of positions 339 and 340, predicted consequence of the frameshift is a nonsense peptide having only the first 10 amino acids.

(Eissenberg and Hartnett, 1993)

Dinucleotide deletion of the last base of the 10th codon and the first base of the 11th codon. The predicted consequence of the frameshift is a peptide having only the first 10 amino acids of the authentic Su(var)205 protein.

(Eissenberg et al., 1992)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

deletion

2L:8,210,813..8,210,814 [-]

Comment:

Dinucleotide deletion of the last base of the 10th codon and the first base of the 11th codon causing a frameshift.

(Eissenberg et al., 1992)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

exacerbates Alzheimer's disease

modeled by Hsap\MAPT^R406W.UAS

(Frost et al., 2014)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal mitotic cell cycle | larval stage (with Su(var)205⁴)

(Cenci et al., 2015)

abnormal neuroanatomy | larval stage (with Su(var)205⁴)

(Kuang et al., 2014)

enhancer of variegation (with Su(var)205²⁰⁵), with lt^unspecified

(Lu et al., 2000)

increased cell death (with Su(var)205⁴)

(Perrini et al., 2004)

lethal | recessive

(Lu et al., 2000, Eissenberg and Hartnett, 1993, Eissenberg et al., 1992)

lethal - all die during embryonic stage | maternal effect | recessive

(Kellum and Alberts, 1995)

non-suppressor of variegation, with P{RS5}5-HA-1005

(Phalke et al., 2009)

non-suppressor of variegation | dominant, with P{hsp26-pt-T}39C-5

(Doheny et al., 2008)

non-suppressor of variegation | dominant, with P{RS5}5-HA-1902

(Phalke et al., 2009)

non-suppressor of variegation | dominant, with P{RS5}5-HA-1992

(Phalke et al., 2009)

some die during embryonic stage | recessive | maternal effect | recessive

(Kellum and Alberts, 1995)

suppressor of variegation

(Schotta et al., 2002)

suppressor of variegation, with Ab(2;3)Tell-P{Winkelried(-FRT)}

(Seum et al., 2000)

suppressor of variegation, with Ab(2;3)Tell-P{Winkelried}D

(Seum et al., 2000)

suppressor of variegation, with In(1)w^m4

(Cleard et al., 1997)

suppressor of variegation, with In(1)w^m4h

(Cobbe et al., 2006)

suppressor of variegation, with In(2)Heidi-P{Winkelried(-FRT)}

(Seum et al., 2000)

suppressor of variegation, with In(2)Heidi-P{Winkelried}D

(Seum et al., 2000)

suppressor of variegation, with P{hsp26-pt-T}118E-10

(Phalke et al., 2009, Cobbe et al., 2006)

suppressor of variegation, with P{w⁺(FRT.attP.1360)}1198

(Sentmanat and Elgin, 2012)

suppressor of variegation, with P{wA^R}A

(Balasov et al., 2000)

suppressor of variegation | dominant

(Apger et al., 2010)

suppressor of variegation | dominant, with dpy^w18/dpy^olv-12

(Chmielewski et al., 2012)

suppressor of variegation | dominant, with In(1)w^m4

(Phalke et al., 2009, Doheny et al., 2008)

suppressor of variegation | dominant, with In(1)w^m4h

(Dorn et al., 1993, Eissenberg and Hartnett, 1993, Eissenberg et al., 1992)

suppressor of variegation | dominant, with P{hsp26-pt-T}8-M76

(Wang et al., 2014)

suppressor of variegation | dominant, with P{hsp26-pt-T}39C-66

(Wang et al., 2014)

suppressor of variegation | dominant, with P{RS3}pan^CB-5204-3

(Phalke et al., 2009)

suppressor of variegation | dominant, with T(2;3)V21-P{lacW}92E.x3

(Phalke et al., 2009)

Phenotype Manifest In

chromosome, telomeric region (with Su(var)205⁴)

(Perrini et al., 2004, Cenci et al., 2003)

chromosome | larval stage (with Su(var)205⁴)

(Cenci et al., 2015)

gastric caecum (with Su(var)205²)

(Spierer et al., 2005)

imaginal disc

(Fanti et al., 1998)

imaginal disc (with Su(var)205⁴)

(Perrini et al., 2004)

larval neuroblast (with Su(var)205⁴)

(Cenci et al., 2015)

meiosis & nuclear chromosome | male

(Fanti et al., 1998)

mitosis & nuclear chromosome (with Su(var)205⁴)

(Cenci et al., 2003)

neuroblast & larval brain

(Fanti et al., 1998)

neuroblast | larval stage (with Su(var)205⁴)

(Kuang et al., 2014)

polytene chromosome (with Su(var)205⁰³)

(Demakova et al., 2007)

polytene chromosome (with Su(var)205⁴)

(Deng et al., 2007)

polytene chromosome | male (with Su(var)205⁴)

(Spierer et al., 2005)

prothoracic gland | larval stage (with Su(var)205²)

(Spierer et al., 2005)

Detailed Description

Statement

Reference

In Su(var)205⁴/Su(var)205⁵ homozygous larvae, neuroblasts exhibit telomeric fusions, particularly in autosomes.

(Cenci et al., 2015)

Severe neuroblast loss is seen in the brains of Su(var)205⁴/Su(var)205⁵ mutant larvae. Many of the remaining neuroblasts exhibit necrosis markers, including aggregation of mitochondria and the presence of ubiquitin-conjugated protein aggregates.

(Kuang et al., 2014)

Su(var)205⁵ dominantly suppresses position effect variegation of a variegating dp allele.

(Apger et al., 2010)

Su(var)205⁵ dominantly suppresses variegation of the w gene caused by In(1)w^m4, T(2;3)V21-P{lacW}92E.x3 and P{RS3}pan^CB-5204-3.

Su(var)205⁵ does not dominantly suppress variegation of the w gene caused by P{RS5}5-HA-1902 and P{RS5}5-HA-1992.

Su(var)205⁵ suppresses variegation of the w gene caused by P{hsp26-pt-T}118E-10.

Su(var)205⁵ does not suppress variegation of the w gene caused by P{RS5}5-HA-1005.

(Phalke et al., 2009)

One copy of Su(var)205⁵ strongly suppresses position effect variegation (PEV) at the w locus caused by In(1)w^m4.

One copy of Su(var)205⁵ is unable to suppress the telomeric position effect (TPE) in stocks carrying a variegating P{hsp26-pt-T}39C-5 insertion at the telomere of the left arm of chromosome two.

(Doheny et al., 2008)

A moderately sized pseudopuff is seen in the area of the 20F-3C eu-heterochromatin junction in the In(1)w^m4h salivary gland polytene chromosome in the Su(var)205⁰³/Su(var)205⁵ mutant background.

(Demakova et al., 2007)

Polytene chromosomes of Su(var)205⁴/Su(var)205⁵ larvae partly lose the distinct pattern of band-interband regions, especially in the male X chromosome.

(Deng et al., 2007)

The X chromosome is bloated in polytene chromosome squashes from male Su(var)205⁴/Su(var)205⁵ third instar larvae.

(Spierer et al., 2005)

The larval imaginal discs of Su(var)205⁵/Su(var)205² mutants are similar to wild-type imaginal discs. Larval imaginal discs of Su(var)205⁴/Su(var)205⁵ mutants exhibit extensive apoptosis. Multiple telomere fusions are seen in metaphase neuroblasts of Su(var)205⁴/Su(var)205⁵ larvae.

(Perrini et al., 2004)

Su(var)205⁴/Su(var)205⁵ larval mitotic chromosomes show telomeric fusions.

(Cenci et al., 2003)

Heterozygotes show a suppression of the position effect variegation seen in In(1)w^m4. This phenotype shows a cooperative effect with the enhancement of PEV caused by the loss of a Y chromosome, resulting in a variegated eye phenotype.

(Schotta et al., 2002)

Heterozygotes show no effect on crossing over between kni and p^p.

(Westphal and Reuter, 2002)

Neuroblasts in the brain of Su(var)205¹/Su(var)205⁵, Su(var)205⁰³/Su(var)205⁵ or Su(var)205⁴/Su(var)205⁵ larvae show a high frequency of abnormal metaphase configurations, including telomere-telomere fusions. Neuroblasts in the brain of Su(var)205²/Su(var)205⁵ larvae show normal metaphase configurations. The imaginal discs of Su(var)205⁴/Su(var)205⁵ larvae are reduced in size. Abnormal metaphase configurations showing telomeric fusions are seen in imaginal disc cells. Abnormal meiotic chromosomes are seen in the male germline.

(Fanti et al., 1998)

Suppresses the position effect variegation at the w locus caused by In(1)w^m4. This suppression is partly reduced if the flies are also carrying Su(var)3-7^+t6.5.

(Cleard et al., 1997)

Homozygous embryos are found to be mostly devoid of intact nuclei. Any remaining nuclei have defects in chromosome segregation and abnormal morphology (chromatin bridges, chromosome lagging during anaphase and under- or over-condensed nuclei). Mitotic synchrony is lost in a high proportion of embryos and defective nuclei are seen beneath the normal plane of nuclei, they appear to be falling into the interior of the embryo.

(Kellum and Alberts, 1995)

mod(mdg4)^neo129 suppresses the strong dominant suppressor effect.

(Dorn et al., 1993)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Su(var)205⁵/Su(var)205⁴ has abnormal neuroanatomy | larval stage phenotype, suppressible | partially by Scer\GAL4^wor.PA/fzy^UAS.cKa

(Kuang et al., 2014)

Su(var)205⁵, Sxl^fP7B0 has partially lethal - majority die | dominant | maternal effect phenotype, suppressible by cav²²⁴⁸

(Li et al., 2011)

Su(var)205⁵, Sxl^fP7B0 has partially lethal - majority die | dominant | maternal effect phenotype, suppressible by cav¹

(Li et al., 2011)

NOT suppressed by

Statement

Reference

Su(var)205⁵/Su(var)205⁴ has lethal phenotype, non-suppressible by JIL-1^z2

(Deng et al., 2007)

Enhancer of

Statement

Reference

Su(var)205⁵/Su(var)205[+] is an enhancer of increased cell death phenotype of Hsap\MAPT^R406W.UAS, Scer\GAL4^elav.PU

(Frost et al., 2014)

Su(var)205⁵/Su(var)205[+] is an enhancer of abnormal locomotor behavior phenotype of Hsap\MAPT^R406W.UAS, Scer\GAL4^elav.PU

(Frost et al., 2014)

Suppressor of

Statement

Reference

Su(var)205⁵/Su(var)205[+] is a suppressor | partially of partially lethal - majority live phenotype of ct^A, lncRNA:flam^A

(Guida et al., 2016)

NOT Suppressor of

Statement

Reference

Su(var)205⁵/Su(var)205[+] is a non-suppressor of lethal | recessive phenotype of JIL-1^z2

(Deng et al., 2007)

Other

Statement

Reference

Dp(1;3)FF184439/+, Su(var)205⁵ has abnormal eye color phenotype

(Jankovics et al., 2018)

Dp(1;2)FF026056/+, Su(var)205⁵ has abnormal eye color phenotype

(Jankovics et al., 2018)

Su(var)205⁵, peo¹/peo[+] has abnormal mitotic cell cycle | larval stage phenotype

(Cenci et al., 2015)

Su(var)205⁵, Sxl^fP7B0 has partially lethal - majority die | dominant | maternal effect phenotype

(Li et al., 2011)

Su(var)205⁵, Sxl^f1 has partially lethal - majority die | dominant | maternal effect phenotype

(Li et al., 2011)

In(1)w^m4h, LamC^EX187, Su(var)205⁵/Su(var)205[+] has suppressor of variegation | dominant phenotype

(Gurudatta et al., 2010)

In(1)w^m4h, LamC^EX296, Su(var)205⁵/Su(var)205[+] has suppressor of variegation | dominant phenotype

(Gurudatta et al., 2010)

P{P-Sal}Pak3^P-Sal, P{lacW}Dplac, Su(var)205⁵ has suppressor of variegation phenotype

(Bushey and Locke, 2004)

Phenotype Manifest In

Suppressed by

Statement

Reference

Su(var)205⁵/Su(var)205⁴ has neuroblast | larval stage phenotype, suppressible | partially by Scer\GAL4^wor.PA/fzy^UAS.cKa

(Kuang et al., 2014)

NOT suppressed by

Statement

Reference

Su(var)205⁵/Su(var)205⁴ has polytene chromosome phenotype, non-suppressible by JIL-1[+]/JIL-1^z2

(Deng et al., 2007)

Enhancer of

Statement

Reference

Su(var)205⁵/Su(var)205[+] is an enhancer of neuron phenotype of Hsap\MAPT^R406W.UAS, Scer\GAL4^elav.PU

(Frost et al., 2014)

NOT Suppressor of

Statement

Reference

Su(var)205⁵/Su(var)205[+] is a non-suppressor of polytene chromosome phenotype of JIL-1^z2

(Deng et al., 2007)

Su(var)205⁵/Su(var)205⁴ is a non-suppressor of polytene chromosome phenotype of JIL-1^z2

(Deng et al., 2007)

Su(var)205⁵ is a non-suppressor of phenotype of w^Sc2

(Netter et al., 1998)

Su(var)205⁵ is a non-suppressor of phenotype of w^cre1

(Netter et al., 1998)

Other

Statement

Reference

Su(var)205⁵, peo¹/peo[+] has larval neuroblast phenotype

(Cenci et al., 2015)

Su(var)205⁵, peo¹/peo[+] has FlyBase_internalproperty type:chromosome:chromosome | larval stage phenotype

(Cenci et al., 2015)

Additional Comments

Genetic Interactions

Statement

Reference

Su(var)205⁵, Dp(1;3)FF184439 and Su(var)205⁵, Dp(1;2)FF026056 double heterozygotes exhibit a significant decrease in eye pigmentation as compared to Su(var)205⁵ single heterozygotes.

(Jankovics et al., 2018)

Su(var)205⁵/+ partially suppresses the increased pharate stage mortality seen in flam^A, ct^A double homozygotes.

(Guida et al., 2016)

peo¹, Su(var)205⁵ double heterozygosity leads to telomeric fusions in larval neuroblasts.

(Cenci et al., 2015)

Expression of fzy^Scer\UAS.cKa under the control of Scer\GAL4^wor.PA suppresses the neuroblast loss seen in Su(var)205⁴/Su(var)205⁵ mutant larval brains. However, these surviving neuroblasts display phenotypes indicative of mitotic catastrophe including multinucleation and enlargement of cell diameter.

(Kuang et al., 2014)

In progeny from Su(var)205⁵ heterozygous females crossed to Sxl^fP7B0/Y males, female viability is dramatically reduced. The effect of Su(var)205⁵ is strictly maternal; no significant reduction in female viability is observed in the reciprocal cross.

In progeny from Su(var)205⁵ heterozygous females crossed to Sxl^f1/Y males, female viability is dramatically reduced. The effect of Su(var)205⁵ is strictly maternal; no significant reduction in female viability is observed in the reciprocal cross.

The introduction of cav²²⁴⁸ into Su(var)205⁵ mothers when crossed to Sxl^fP7B0 males suppresses the Su(var)205⁵ maternal effect on female viability in the progeny.

The introduction of cav¹ into Su(var)205⁵ mothers when crossed to Sxl^fP7B0 males suppresses the Su(var)205⁵ maternal effect on female viability in the progeny.

(Li et al., 2011)

The defects in polytene chromosome morphology seen in Su(var)205⁴/Su(var)205⁵ larvae are not altered by one copy of JIL-1^z2.

The defects in polytene chromosome morphology seen in JIL-1^z2 homozygous larvae are not altered by one copy of Su(var)205⁴ or Su(var)205⁵.

The polytene chromosome morphology of Su(var)205⁴/Su(var)205⁵ ; JIL-1^z2/JIL-1^z2 double mutants resembles that of JIL-1^z2/JIL-1^z2 single mutants.

(Deng et al., 2007)

When P{P-Sal}89D is present, the w gene of the P{lacW}ci^Dplac insertion variegates, resulting (in a w^- background) in an eye that is mostly white with a few patches of red ommatidia. This P-element-dependent silencing of P{lacW}ci^Dplac is dominantly suppressed by Su(var)205⁵.

(Bushey and Locke, 2004)

Su(var)205⁵ Df(3R)Ace-HD1 double heterozygotes show an increase in crossing over between kni and p^p. Su(var)205⁵ Su(var)3-9⁶ double heterozygotes show an increase in crossing over between kni and p^p.

(Westphal and Reuter, 2002)

Xenogenetic Interactions

Statement

Reference

Heterozygous Su(var)205⁵ enhances the Scer\GAL4^elav.PU>Hsap\MAPT^{R406W.Scer\UAS} induced neuronal apoptosis and locomotory defects.

(Frost et al., 2014)

Complementation and Rescue Data

Fails to complement

Su(var)205^P4

(Bushey and Locke, 2004)

Su(var)205¹⁴⁹

(Lu et al., 2000)

Rescued by

Su(var)205⁵ is rescued by Su(var)205^hs.PE

(Kellum and Alberts, 1995)

Partially rescued by

Su(var)205⁵/Su(var)205⁴ is partially rescued by Su(var)205^{Tag:BTS,Tag:proteinA}

(Alekseyenko et al., 2014)

Su(var)205⁵/Su(var)205⁴ is partially rescued by Su(var)205^hs.PE

(Fanti et al., 1998)

Comments

Su(var)205^{T:Zzzz\BTS,T:Saur\A} rescues the lethality of Su(var)205⁴/Su(var)205⁵ to 58%.

(Alekseyenko et al., 2014)

The abnormal metaphase configurations seen in the brains of Su(var)205⁴/Su(var)205⁵ larvae are partly rescued by expression of Su(var)205^hs.PE, although a high frequency of chromosome aberrations are seen.

(Fanti et al., 1998)

Lethality is rescued by expression of the heat shock activated Su(var)205 construct. Rescued adults behave lethargically, walk slowly and never fly, and die several days after eclosion. If heat shock is induced after 3-4 days adults are rescued but heat shock after 5-6 days adults exhibit mild to severe defects in eye and ocellar development.

(Eissenberg and Hartnett, 1993)

Images (0)

Mutant

Wild-type

Stocks (3)

Bloomington

6234

In(1)w^m4h; Su(var)205⁵/In(2L)Cy, In(2R)Cy, Duox^Cy

Kyoto

108692

In(1)w^m4h; Su(var)205⁵/In(2L)Cy, In(2R)Cy, Duox^Cy

103332

In(1)w^m4h ; Su(var)205⁵ / In(2L)Cy, In(2R)Cy, Duox^Cy

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (8)

Reported As

Symbol Synonym

HP1⁰⁵

(Johansson et al., 2007)

Hp1⁵

(Chmielewski et al., 2012, Apger et al., 2010)

Su(var)2-5⁵

(Hong and Choi, 2016, Vasanthi et al., 2013, Doheny et al., 2008, Balasov et al., 2000, Eissenberg and Hartnett, 1993, Eissenberg et al., 1992)

Su(var)205-5

(Netter et al., 1998)

Su(var)205⁰⁵

(Porrazzo et al., 2022, Schoelz et al., 2021, Cenci et al., 2015, Xing and Li, 2015, Alekseyenko et al., 2014, Wang et al., 2014, Silva-Sousa and Casacuberta, 2013, Sentmanat and Elgin, 2012, Shaffer et al., 2006, Bi et al., 2005, Raffa et al., 2005)

Su(var)205⁵

(Delanoue et al., 2023, Martins et al., 2019, Jankovics et al., 2018, Sun et al., 2018, Roelens et al., 2017, Giauque and Bickel, 2016, Guida et al., 2016, Xing and Li, 2015, Frost et al., 2014, Kuang et al., 2014, Ito et al., 2012, Dufourt et al., 2011, Kurzhals et al., 2011, Li et al., 2011, Gurudatta et al., 2010, Chen et al., 2008, Josse et al., 2007, Shaffer et al., 2006)

Suvar2-5⁰⁵

(Kellum and Alberts, 1995)

Name Synonyms

Secondary FlyBase IDs

References (71)

Report Sections

Open Close