|Feature type||allele||Associated gene||Dmel\sws|
|Map ( GBrowse )|
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|Nature of the Allele|
|Mutations Mapped to the Genome|
|Associated Sequence Data|
|Nature of the lesion|
The sws1 mutation produces a truncated polypeptide that terminates at residue 375, thus lacking the esterase domain.
Amino acid replacement: S375@. Nucleotide substitution: C1616A.
|Phenotype Manifest In|
14 day old vap[KS67] mutant flies show vacuoles in all parts of the brain. These increase dramatically with age.
sws1 flies exhibit vacuolisation in the neuropil (due to degeneration of neurites) and the formation of membranous glial bodies. sws1 flies exhibit a strong glial phenotype consisting of the formation of multilayered glial wrappings and subsequent glial cell death.
Adults have vacuoles in all brain regions. This degeneration increases with age, as shown by an increased number of vacuoles and shrinking of the cell cortex. An increasing amount of cell death is also seen in the brain. Compact dark bodies are seen in the cortex, especially the lamina cortex. These structures are first seen during late pupal stages, and increase in size during aging of the adult. Approximately 10% of brain cortex neurons are enwrapped by multilayered membranes originating from nearby glia in newly eclosed flies. Membranous whorls are seen in older flies. Homozygotes adults show a significant reduction in lifespan compared to wild-type flies.
many Swiss-cheese-like holes seen in brain sections of adults (similar to phenotype observed in dying drd flies). Phenotype has become less extreme, apparently due to accumulation of modifiers (Heisenberg).
sws1 has neuroanatomy defective | adult stage phenotype, suppressible by Scer\GAL4elav-C155/Appls.Scer\UAS.T:Ivir\HA1
sws1 has neuroanatomy defective | adult stage phenotype, suppressible by Scer\GAL4elav-C155/ApplScer\UAS.cCSa
|Phenotype Manifest In|
Expression of Appl[s.Scer\UAS.T:Ivir\HA1] under the control of Scer\GAL4[elav-C155] fails to suppress the vacuolisation seen in the brains of sws mutant 14 day old males. Expression of Appl[Scer\UAS.cCSa] under the control of Scer\GAL4[elav-C155] fails to suppress the vacuolisation seen in the brains of sws mutant 14 day old males.
Neuronal expression of Mmus\NTEScer\UAS.cMa, under the control of Scer\GAL4elav-C155 rescues some aspects of the neuronal degeneration, namely the vacuolisation in the neuropil. Glial expression of Mmus\NTEScer\UAS.cMa, under the control of Scer\GAL4loco.1.3 rescues the glial phenotype. Expression of Mmus\NTEScer\UAS.cMa in either neurons or glia (under the control of either Scer\GAL4elav-C155 or Scer\GAL4loco.1.3) restores the wild-type NTE-like activity (NTE-neuropathy target esterase).
|Complementation & Rescue Data|
|Not rescued by|
Neuronal expression of swsScer\UAS.cMa, under the control of Scer\GAL4elav-C155 rescues some aspects of sws1 neuronal degeneration, namely the vacuolisation in the neuropil. Glial expression of swsScer\UAS.cMa, under the control of Scer\GAL4loco.1.3 rescues the glial phenotype found in sws1 mutants. Expression of swsScer\UAS.cMa in either neurons or glia (under the control of either Scer\GAL4elav-C155 or Scer\GAL4loco.1.3) restores the wild-type NTE-like activity (NTE-neuropathy target esterase).
|Stocks ( 0 )|
|Notes on Origin|
Selected as: Anatomical defect (see FBrf0033934).
|External Crossreferences & Linkouts|
|Synonyms & Secondary IDs ( 2 )|
|Secondary FlyBase IDs|
|References ( 3 )|