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General Information
Symbol
Dmel\Sxlfhv1
Species
D. melanogaster
Name
FlyBase ID
FBal0016688
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
Sxlfhv#1
Mutagen
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

Approximately 6 kb insert present.

Insertion of 6kb DNA into Sxl. Insertion site is approximately 6-6.5kb distal to the site of P-element insertion in SxlfPb.

Insertion components
?{}Sxlfhv1
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In

intercalary heterochromatin & polytene chromosome | female (with Sxlf1)

Detailed Description
Statement
Reference

The salivary gland cells of Sxlf1/Sxlfhv1 females show somatic mosaicism; some cells are of "male" type (Sxl is not expressed), while others are of "female" type (Sxl is expressed). The frequency of weak spots in the intercalary heterochromatin regions of the X chromosomes is much lower in the "male" cells compared to in the "female" cells and bands of intercalary heterochromatin in the X chromosome look solid, dense and non-broken in the "male" cells, as occurs in normal males.

A few percent of the Sxlf1/Sxlfhv1 female progeny survive to the third larval instar stage.

Females of the genotype Sxlf1/Sxlfhv1 fail to activate the Sxl locus in a subset of their cells and thus are mosaic for cells that follow either the male of female pathway for dosage compensation. In XX cells that have adopted the male fate msl-2 interacts in a full male-like pattern. Mosaics lacking mle or msl-3 show partial immunostaining patterns for msl-2. msl-1 and msl-2 precisely colocalise at the msl-3 and mle independent sites. msl-2 localisation is completely abolished in the absence of msl-1.

Heterozygous Sxlf1/Sxlfhv1 female larvae are mosaics of male and female cells.

50% females heteroallelic for Sxlf1 and Sxlfhv1 survive to the third instar larval stage and are mosaic for Sxl expression.

Viability of Sxlf1/Sxlfhv1 females reduced compared to wild type, but some individuals survive into larvae and a few survive to adult hood. The larvae are mosaic for Sxl function: Sxl protein is bound to X chromosomes in approximately half of the cells in the salivary glands, mle protein is bound to X chromosomes in those cells where Sxl is not. Sxlf1/Sxlfhv1 females that are also homozygous for mutants at msl-1, msl-2 or msl-3 develop as intersexes of the mosaic type.

A subliminal allele, viable and fertile as homozygous females, but with greatly reduced viability in trans to nulls. Polytene chromosomes of Sxlfhv1/Sxlf1 larvae that survive to third instar hyperincorporate uridine, revealing female dosage compensation upsets. Mutation of mle appears to partially masculinize this heteroallelic combination and may slightly increase viability under some conditions. Sxlfhv1 homozygotes and heterozygotes display an increased requirement for maternal da+ activity, suggestive of defects in early Sxl regulation.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
NOT suppressed by
Statement
Reference

Sxlfhv1/Sxlf1 has lethal | female phenotype, non-suppressible by Low1

Other
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
Comments
Comments

Nuclei follow a female pathway, elevated H4Ac16 staining, acetylated form of His4, is not detected in the paired X chromosome.

Individuals of heteroallelic combination Sxlfhv1 and Sxlf1 have reduced Sxl levels.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Name Synonyms
Secondary FlyBase IDs
    References (23)