Allele Dmel\amos^Tft

General Information
Symbol	Dmel\amos^Tft	Species	D. melanogaster
Name	Tufted	FlyBase ID	FBal0016764
Feature type	allele	Associated gene	Dmel\amos
Also Known As	Tft¹, Tft

Allele class	gain of function allele
Mutagen	X ray
Recent Updates
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FB2013_03
FB2013_02
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Nature of the Allele
Allele class	gain of function allele (Villa-Cuesta et al., 2003)
Mutagen	X ray
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Duplication and translocation of sequences from 36F3-36F7 to 37A. The proximal limit of the duplication has not been determined, but extends a minimum of 75kb upstream of amos. The distal limit of the duplication terminates in the vicinity of amos but its structure is complex. (Lai, 2003) Small rearrangement of the amos chromosomal region, most probably a duplication of several kilobases including the amos gene. (Villa-Cuesta et al., 2003)
Caused by aberration	Dp(2;2)amos^Tft (Villa-Cuesta et al., 2003, Lai, 2003)
Carried on aberration	T(Y;2)TW20
Cytology
Phenotypic Data
Phenotypic Class
	visible \| dominant (Villa-Cuesta et al., 2003, Lai, 2003, Sur et al., 1995) visible \| recessive (Held, 1990)
Phenotype Manifest In
	abdominal dorsal bipolar neuron dbp \| ectopic (Villa-Cuesta et al., 2003) adult mesothoracic segment (Villa-Cuesta et al., 2003) antennal sense organ \| ectopic (Villa-Cuesta et al., 2003) chaeta \| ectopic (Villa-Cuesta et al., 2003) dorsal abdominal cluster (Villa-Cuesta et al., 2003) dorsocentral bristle \| supernumerary (Sur et al., 1995) macrochaeta \| ectopic (Villa-Cuesta et al., 2003, Lai, 2003) mesothoracic metatarsus (Held, 1990) mesothoracic tarsal bristle (Held, 1990) mesothoracic tergum (Lai, 2003) metathoracic laterotergite (Villa-Cuesta et al., 2003, Lai, 2003) microchaeta \| ectopic (Lai, 2003) postalar bristle \| supernumerary (Sur et al., 1995) scutellar bristle \| supernumerary (Sur et al., 1995) scutellum (Villa-Cuesta et al., 2003, Lai, 2003) sensory mother cell \| ectopic (Villa-Cuesta et al., 2003, Lai, 2003) tormogen cell (Villa-Cuesta et al., 2003) wing disc (Villa-Cuesta et al., 2003, Lai, 2003)
Detailed Description
	Statement Reference Heterozygotes have many ectopic macrochaetae and microchaetae. Ectopic bristles are particularly seen in the postalar, dorsocentral and scutellar regions of the notum. Tufts of closely spaced or even adjacent bristles are seen. Ectopic bristles are also seen in the anterior-central portion of the scutellum and the metathoracic notum. Ectopic sensory organ precursor cells form in the presumptive posterior notum and scutellum of heterozygous wing discs. (Lai, 2003) Heterozygotes and homozygotes have extra bristles mainly in the postalar, dorsocentral and scutellar regions of the dorsal mesothorax. Ectopic bristles and other sensory organs are also formed in the metanotum, dorsal to the halteres, in heterozygotes. The extra bristles seen in heterozygotes and homozygotes can develop very close together, forming tufts, and in some cases several tormogens can be fused together. Heterozygotes and homozygotes have a reduced scutellum. The average number of ectopic macrochaetae per heminotum is 23 +/- 3 in heterozygous flies. Ectopic sensory bristles are occasionally seen in the antennae of heterozygous flies, while the arrangement of the olfactory sensilla is essentially normal. A large number of extra sensory organ precursor cells (SOPs) form in the presumptive posterior notum in the wing disc in heterozygotes. These extra SOPs form very close together and their appearance is sequential and occurs concomitantly with the normal SOPs. amos^Tft embryos show an increase in the number of neurons in the dorsal cluster of the peripheral nervous system. Occasionally the dorsal bipolar neuron is duplicated. (Villa-Cuesta et al., 2003) Heterozygotes have an increased number of bristles in the postalar (13-18), dorsocentral (5-10) and scutellar (7-10) regions of the thorax (normal numbers are 4-5, 3-4 and 4-5 respectively). (Sur et al., 1995) The total number of bristles on the basitarsi of the second legs is slightly increased. (Held, 1990) Extra bristles often very close together; underlying neurons make functional contacts with the CNS (Ghysen and Richelle, 1979). Innervated bristles also found in the metanotum, which has no bristles in wild-type flies (Garcia-Bellido and Deak). Dominant mutation characterized by an increased number of bristles in the postalar, dorsocentral and scutellar regions; tufts of bristles formed on mesothorax in both homo- and heterozygotes; bristles shorter in homozygotes. Extra bristles located dorsal to halteres at junction of thorax and abdomen. Posterior part of mesonotum appears wider than normal. Homo- and heterozygous females have a greatly reduced scutellum; scutoscutellar suture almost absent; heterozygous males have a nearly normal scutellum (Arnheim, 1967). Small to moderate amounts of fluid tend to remain between the epithelial layers of the wing. Penetrance of extra-bristle character 100%. amos not suppressed by Df(1)sc19 but suppressed by Df(1)260-1 (Garcia-Bellido, communicated to Campuzano, Carramolino, Cabrera, Ruiz-Gomez, Villares, Boronat and Modolell, 1985). Cell autonomous in mosaics (Arnheim, 1967). Viability and fertility low.
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement Reference amos^Tft has visible \| dominant phenotype, enhanceable by Brd[+]/Brd¹ (Lai, 2003) amos^Tft has visible \| dominant phenotype, enhanceable by Dl^M1/Dl[+] (Villa-Cuesta et al., 2003) amos^Tft has visible \| dominant phenotype, enhanceable by emc¹/emc¹¹ (Villa-Cuesta et al., 2003)
Suppressed by
Statement Reference amos^Tft has visible \| dominant phenotype, suppressible \| partially by da[+]/da¹⁰ (Lai, 2003) amos^Tft has visible \| dominant phenotype, suppressible \| partially by emc^D/emc^D (Villa-Cuesta et al., 2003) amos^Tft has visible \| dominant phenotype, suppressible \| partially by H²/H[+] (Villa-Cuesta et al., 2003) amos^Tft has visible \| dominant phenotype, suppressible \| partially by N^Ax-M1/N[+] (Villa-Cuesta et al., 2003) amos^Tft has visible \| dominant phenotype, suppressible \| partially by Scer\GAL4^C-765/E(spl)m8-HLH^Scer\UAS.cSa (Villa-Cuesta et al., 2003) amos^Tft has visible \| dominant phenotype, suppressible by Df(1)ase-1 (Villa-Cuesta et al., 2003)
NOT suppressed by
Statement Reference amos^Tft has visible \| dominant phenotype, non-suppressible by Df(1)sc10-1 (Lai, 2003, Villa-Cuesta et al., 2003)
Phenotype Manifest In
Enhanced by
Statement Reference amos^Tft has macrochaeta \| ectopic phenotype, enhanceable by Brd[+]/Brd¹ (Lai, 2003) amos^Tft has macrochaeta \| ectopic phenotype, enhanceable by Dl^M1/Dl[+] (Villa-Cuesta et al., 2003) amos^Tft has macrochaeta \| ectopic phenotype, enhanceable by emc¹/emc¹¹ (Villa-Cuesta et al., 2003)
NOT Enhanced by
Statement Reference amos^Tft has phenotype, non-enhanceable by ac^T52.2 (Lai, 2003)
Suppressed by
Statement Reference amos^Tft has macrochaeta \| ectopic phenotype, suppressible \| partially by da[+]/da¹⁰ (Lai, 2003) amos^Tft has macrochaeta \| ectopic phenotype, suppressible \| partially by emc^D/emc^D (Villa-Cuesta et al., 2003) amos^Tft has macrochaeta \| ectopic phenotype, suppressible \| partially by H²/H[+] (Villa-Cuesta et al., 2003) amos^Tft has macrochaeta \| ectopic phenotype, suppressible \| partially by N^Ax-M1/N[+] (Villa-Cuesta et al., 2003) amos^Tft has macrochaeta \| ectopic phenotype, suppressible \| partially by Scer\GAL4^C-765/E(spl)m8-HLH^Scer\UAS.cSa (Villa-Cuesta et al., 2003) amos^Tft has macrochaeta \| ectopic phenotype, suppressible by Df(1)ase-1 (Villa-Cuesta et al., 2003)
NOT suppressed by
Statement Reference amos^Tft has macrochaeta \| ectopic phenotype, non-suppressible by Df(1)sc10-1 (Lai, 2003, Villa-Cuesta et al., 2003)
Suppressor of
Statement Reference amos^Tft is a suppressor of microchaeta \| ectopic & scutellum phenotype of h¹ (Villa-Cuesta et al., 2003)
Additional Comments
Genetic Interactions
Statement Reference The amos^Tft/+ phenotype ectopic bristle phenotype is enhanced by Brd¹/+. amos^Tft is epistatic to Df(1)sc10-1/Y - Df(1)sc10-1/Y ; amos^Tft/+ flies have ectopic sensory organs in the regions seen in amos^Tft/+ animals. (Lai, 2003) The average number of ectopic macrochaetae per heminotum in amos^Tft/+ flies is increased to 35 +/- 6 if they are also carrying emc¹/emc¹¹. The average number of ectopic macrochaetae per heminotum in amos^Tft/+ flies is increased to 33 +/- 5 if they are also carrying Dl^M1/+. The average number of ectopic macrochaetae per heminotum in amos^Tft/+ flies is reduced to 16 +/- 4 if they are also carrying N^Ax-M1/+. The average number of ectopic macrochaetae per heminotum in amos^Tft/+ flies is reduced to 19 +/- 2 if they are also carrying H²/+. The average number of ectopic macrochaetae per heminotum in amos^Tft/+ flies is reduced to 6 +/- 2 if they are also expressing E(spl)^Scer\UAS.cSa under the control of Scer\GAL4^C-765. The average number of ectopic macrochaetae per heminotum in amos^Tft/+ flies is reduced to 4 +/- 2 if they are also carrying emc^D/emc^D. The ectopic microchaetae seen on the scutellum in h¹ flies are suppressed by amos^Tft. The tufts of ectopic bristles on the notum that are seen in amos^Tft/+ flies are suppressed by Df(1)ase-1, but are unaffected by Df(1)sc10-1. (Villa-Cuesta et al., 2003)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 36 )
Bloomington	424 amos^Tft/CyO 6118 Df(2L)J3, b¹ amos^Tft/CyO 6119 Df(2L)J16, b¹ amos^Tft/CyO 3782 Df(2L)TW9, amos^Tft cn¹/CyO 3184 amos^Tft hk¹ pr¹/CyO 3780 Df(2L)TW2, amos^Tft l(2)74i¹/CyO
Kyoto	105904 amos^Tft/CyO 106524 amos^Tft P{neoFRT}40A/CyO 101432 Df(2L)TW1, amos^Tft l(2)74i / CyO 108618 Df(2L)J3, b¹ amos^Tft/CyO 108619 Df(2L)J16, b¹ amos^Tft/CyO 107230 Df(2L)TW9, amos^Tft cn¹/CyO
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Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 4 )
Reported As
Symbol Synonym	amos^Tft (Grau et al., 2008) Tft (Held, 1990, Sur et al., 1995) Tft¹ (Lai, 2003, Villa-Cuesta et al., 2003, )
Name Synonym	Tufted (Sur et al., 1995, )
Secondary FlyBase IDs

References ( 7 )
Research paper	Grau et al., 2008, Mol. Cell. Biol. 28(1): 376--385 Transcriptional adaptor ADA3 of Drosophila melanogaster is required for histone modification, position effect variegation, and transcription. [FBrf0203033] Lai, 2003, Genetics 163(4): 1413--1425 Drosophila Tufted is a gain-of-function allele of the proneural Gene amos. [FBrf0158982] Villa-Cuesta et al., 2003, Genetics 163(4): 1403--1412 Tufted is a gain-of-function allele that promotes ectopic expression of the proneural gene amos in Drosophila. [FBrf0158981] Sur et al., 1995, D. I. S. 76: 152--153 Epigenetic interaction between Scutoid (Sco) and Tufted (Tft) of Drosophila melanogaster. [FBrf0082672] Held, 1990, Rouxs Arch. Dev. Biol. 199(1): 48--62 Arrangement of bristles as a function of bristle number on a leg segment in Drosophila melanogaster. [FBrf0052925] Wright et al., 1976, Genetics 84: 267--285 The genetics of dopa decarboxylase in Drosophila melanogaster. [FBrf0028752]
Book	Lindsley and Zimm, 1992, The Genome of Drosophila melanogaster. The Genome of Drosophila melanogaster. [FBrf0066905]

Allele Dmel\amosTft

Allele Dmel\amos^Tft