Eggs from tkv⁷/tkv³ females fertilised with tkv⁷ sperm develop into ventralised embryos comparable to a dpp null mutant.

Embryos derived from a cross of tkv³/tkv⁷ females to tkv⁷ Df(2L)OD16/+ males differentiate 3 classes of cuticle; 25% (of putative genotype tkv⁷/tkv⁷ Df(2L)OD16) have a phenotype identical to that of dpp null mutants, 25% (of putative genotype tkv³/tkv⁷ Df(2L)OD16) are ventralised with expansion of ventral denticle bands and internalised fusion of the filzkorper, and the remaining embryos (which contain a wild-type copy of tkv) differentiate more weakly ventralised cuticles. Injection of embryos derived from a cross of tkv³/tkv⁷ females to tkv⁷ Df(2L)OD16/+ males with sax::tkv^{sax.EC.tkv.IC} RNA rescues amnioserosa formation in 91% of embryos that contain a paternal copy of tkv⁷. Injection of embryos derived from a cross of tkv⁷ Df(2L)OD16/tkv³ females to tkv⁷ Df(2L)OD16/+ males with sax::tkv^{sax.EC.tkv.IC} RNA results in 53% of embryos with restored amnioserosa formation and 47% of embryos without amnioserosa formation.

homozygous lethal