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General Information
Symbol
Dmel\tkv7
Species
D. melanogaster
Name
FlyBase ID
FBal0016824
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
tkvIO, tkvstr-I, tkvstrID
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
point mutation
Nucleotide change:
G5220203A
Amino acid change:
E528K | tkv-PA; E496K | tkv-PB; E540K | tkv-PC; E474K | tkv-PD
Reported amino acid change:
E474K
Comment:
Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference
Amino acid replacement: E528K. E528 is a conserved residue that falls in the C terminus of the kinase domain.
Amino acid replacement: E474K.
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference
tkv7/+ adults do not have any obvious wing phenotypes.
Third instar larvae heterozygous for tkv7 show no significant differences in the number or size of neuromuscular junction boutons compared to wild-type controls.
The neuromuscular junction synapses of homozygous tkv7/tkvk16713 mutants are undergrown but the total number of boutons is similar to heterozygous controls.
tkv7/+ mutant wings show thickened, bifurcated L2, L3 and L4 veins, and incomplete anterior crossveins.
The anterior and posterior crossveins are absent and the distal end of vein L4 is lost in tkv427/tkv7 wings.
tkv1/tkv7 animals survive until the third larval instar and show a dramatic reduction in neuromuscular junction (NMJ) size compared to wild type.
The average number of crystal cells per embryo is significantly reduced in homozygous stage 13-14 embryos compared to wild type.
When homozygous clones are made in the developing wing disc, clones in the medial wing pouch survive 36 hours, but are lost within 48 hours of induction. In lateral areas survival is greater, but clones are still small compared to wild-type twin spots. When homozygous tkv7 wing discs are made (in a Minute background) about 30% of discs show an aberrant morphology. tkv7 cells show a cell cycle profile with a very reduced S phase fraction, and an increased G1 fraction. However, mutant cells are not detectably different in size to controls.
tkvk16713/tkv7 larvae show a significant decrease in bouton number at the neuromuscular junction.
tkv7/tkv427 flies have ectopic vein fragments around the distal part of vein L2. Does not suppress the phenotype caused by tkvCA.Scer\UAS expressed under the control of Scer\GAL471B.
A fraction of tkv7/tkv427 flies show a small notching of the wing tip. The number of dying cells in the late third larval instar tkv7/tkv427 wing disc is increased compared to wild type; a cluster of dying cells appears in the primordial wing tip.
The Malpighian tubules of homozygous embryos elongate properly to the two cell circumference, although they are located abnormally in the embryo, coiling around their point of origin from the hindgut.
Eggs from tkv7/tkv3 females fertilised with tkv7 sperm develop into ventralised embryos comparable to a dpp null mutant.
Homozygous clones induced in the pleura cause no mutant phenotype.
Eggs laid by tkv7/+ show 97.2% wild type dorsal appendages, 0.5% broad dorsal appendages and short egg length, 05% broad dorsal appendages and normal egg length and 1.9% abnormal dorsal appendages with short egg length.
Epidermal cells initially appear to elongate normally in dorsal closure, but their migration becomes directed in an anterior-posterior direction, instead of dorsally. Cells of several segments are drawn together into points of focus in regions of the leading edge, pulling the intervening segments apart. This results in epidermal bunching. The embryos have a dorsal open phenotype.
Many tracheal dorsal branches are reduced or absent in the embryo. Where dorsal branches do form they never fuse.
Embryos derived from a cross of tkv3/tkv7 females to tkv7 Df(2L)OD16/+ males differentiate 3 classes of cuticle; 25% (of putative genotype tkv7/tkv7 Df(2L)OD16) have a phenotype identical to that of dpp null mutants, 25% (of putative genotype tkv3/tkv7 Df(2L)OD16) are ventralised with expansion of ventral denticle bands and internalised fusion of the filzkorper, and the remaining embryos (which contain a wild-type copy of tkv) differentiate more weakly ventralised cuticles. Injection of embryos derived from a cross of tkv3/tkv7 females to tkv7 Df(2L)OD16/+ males with sax::tkvsax.EC.tkv.IC RNA rescues amnioserosa formation in 91% of embryos that contain a paternal copy of tkv7. Injection of embryos derived from a cross of tkv7 Df(2L)OD16/tkv3 females to tkv7 Df(2L)OD16/+ males with sax::tkvsax.EC.tkv.IC RNA results in 53% of embryos with restored amnioserosa formation and 47% of embryos without amnioserosa formation.
Homozygous embryos derived from tkv7/tkv3 females lack the dorsal vessel.
Tracheal tree defects consisting mainly of a failure to develop some particular branches in the dorsoventral axis. Other tracheal branches form in the anteroposterior axis, though they display an occasional gap.
tkv1/tkv7 flies have some wing vein thickening, and lack distal stretches of vein LIV.
P{tkvBrk25D1} has suboptimal levels of expression sufficient to rescue the lethality of tkv7/Df(2L)tkv2 through embryonic and larval development. Rescued adults display defects in the wing (cleft in the notum and reduction/deletion of the scutellum) and leg (duplication of ventral structures and loss of dorsal and distal structures) patterning.
Thickened vein mutant.
Heterozygous females that are also heterozygous for dpphr56 or dpphr27, show markedly reduced viability (for dpphr56) or lethality (for dpphr27). The enhancement of the mutant phenotype extends to the phenotype of the embryos produced. Transheterozygotes of tkv7 and sax1 are virtually sterile.
The presence of tkv7 in the mother causes dpphr27 to behave as a dominant lethal in the zygote.
Homozygotes display dorsally open cuticle, severe defective head and contracted epidermis giving the cuticle a rounded appearance. Lethal when heterozygous with tkv6. Female transheterozygotes with tkv1 are viable and display a wing phenotype, portions of veins and crossveins are lost, transheterozygotes with Df(2L)tkv3 display extremely thickened veins. Adult Df(2L)tkv3/tkv7 females lay eggs, embryos from these females crossed to wild type males die showing a moderately ventralised phenotype.
homozygous lethal
External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement
Reference
tkv7/tkv427 has visible phenotype, enhanceable by pucE69
tkv7/tkv427 has visible phenotype, enhanceable by hepCA
Enhancer of
Statement
Reference
tkv7/tkv[+] is an enhancer of visible | adult stage phenotype of Irk2UAS.cDa, Scer\GAL4Bx-MS1096
tkv7/tkv[+] is an enhancer of visible | adult stage phenotype of Irk2DN.UAS, Scer\GAL4Bx-MS1096
NOT Enhancer of
Statement
Reference
tkv7/tkv[+] is a non-enhancer of visible phenotype of Caf1-105NIG.12892R, Scer\GAL4ey.PH
Suppressor of
Statement
Reference
tkv7/tkv[+] is a suppressor | partially of visible | adult stage phenotype of Scer\GAL4A9, anchorGD3613
tkv7/tkv[+] is a suppressor of neuroanatomy defective | larval stage phenotype of Acsl05847/AcslKO
tkv7/tkv[+] is a suppressor of neuroanatomy defective phenotype of spin10403
tkv7/tkv[+] is a suppressor | partially of neuroanatomy defective phenotype of spictmut
tkv7/tkv[+] is a suppressor | partially of visible phenotype of Scer\GAL4A9, gbbUAS.cKa
tkv7/tkv[+] is a suppressor of visible phenotype of Scer\GAL4A9, dppUAS.cHa
tkv7/tkv[+] is a suppressor of visible phenotype of Scer\GAL4Tub.PU, cswN308D.UASp
NOT Suppressor of
Statement
Reference
Other
Phenotype Manifest In
Enhanced by
Statement
Reference
tkv7/tkv427 has wing phenotype, enhanceable by pucE69
tkv7/tkv427 has wing disc phenotype, enhanceable by pucE69
tkv7/tkv427 has wing phenotype, enhanceable by hepCA
Suppressed by
Enhancer of
Statement
Reference
tkv7/tkv[+] is an enhancer of wing phenotype of Irk2DN.UAS, Scer\GAL4Bx-MS1096
NOT Enhancer of
Statement
Reference
tkv7/tkv[+] is a non-enhancer of eye phenotype of Caf1-105NIG.12892R, Scer\GAL4ey.PH
tkv7/tkv[+] is a non-enhancer of wing vein phenotype of Irk2UAS.cDa, Scer\GAL4Bx-MS1096
tkv7/tkv[+] is a non-enhancer of dorsal appendage phenotype of bwk08482/bwk151
Suppressor of
Statement
Reference
tkv7/tkv[+] is a suppressor | partially of wing vein phenotype of Scer\GAL4A9, anchorGD3613
tkv7/tkv[+] is a suppressor | partially of wing phenotype of Scer\GAL4A9, anchorGD3613
tkv7/tkv[+] is a suppressor | partially of germarium phenotype of Cul2GD8913, Scer\GAL4ptc.PU
tkv7/tkv[+] is a suppressor | partially of spectrosome phenotype of Cul2GD8913, Scer\GAL4ptc.PU
tkv7/tkv[+] is a suppressor of NMJ bouton | third instar larval stage phenotype of Rab81/Rab8B229
tkv7/tkv[+] is a suppressor of NMJ bouton | supernumerary phenotype of S6KL140
tkv7/tkv[+] is a suppressor of neuromuscular junction phenotype of Acsl05847/AcslKO
tkv7/tkv[+] is a suppressor of NMJ bouton | supernumerary phenotype of Acsl05847/AcslKO
tkv7/tkv[+] is a suppressor of A2 neuron phenotype of Acsl05847/AcslKO
tkv7/tkv[+] is a suppressor of A3 neuron phenotype of Acsl05847/AcslKO
tkv7/tkv[+] is a suppressor | partially of wing vein phenotype of DdG0269
tkv7/tkv[+] is a suppressor of eye disc phenotype of spin10403
tkv7/tkv[+] is a suppressor of glial cell phenotype of spin10403
tkv1/tkv7 is a suppressor of eye disc phenotype of spin10403
tkv1/tkv7 is a suppressor of glial cell phenotype of spin10403
tkv7/tkv[+] is a suppressor | partially of wing vein phenotype of Scer\GAL4A9, gbbUAS.cKa
tkv7/tkv[+] is a suppressor of wing phenotype of Scer\GAL4A9, dppUAS.cHa
tkv7/tkv[+] is a suppressor of wing vein | ectopic phenotype of Scer\GAL4Tub.PU, cswN308D.UASp
tkv7/tkv[+] is a suppressor of bouton | supernumerary phenotype of spink09905/spin10403
NOT Suppressor of
Statement
Reference
tkv7/tkv[+] is a non-suppressor of NMJ bouton | larval stage phenotype of DAAMEx68
tkv7/tkv[+] is a non-suppressor of LL1 motor neuron | larval stage phenotype of DAAMEx68
tkv7/tkv[+] is a non-suppressor of dorsal appendage phenotype of bwk08482/bwk151
tkv7 is a non-suppressor of phenotype of Src42ASu(Raf)1-1
Other
Additional Comments
Genetic Interactions
Statement
Reference
Addition of tkv7/+ to animals expressing anchorGD3613 under the control of Scer\GAL4A9 partially suppresses the thickened wing vein phenotype, but does not suppress the increased wing size phenotype.
The increased bouton and satellite bouton number characteristic for third instar larvae expressing two copies of Nlg4Scer\UAS.cZa under the control of Scer\GAL4RapGAP1-OK6 is suppressed by combination with a single copy of tkv7. Although none of the larvae heterozygous for either Nlg4KO10 or tkv7 display any significant difference in the number or size of boutons on neuromuscular junctions compared to controls, tkv7/+;Nlg4KO10/+ double heterozygotes all show reduced bouton number and increased bouton size.
The increased number of spectrosome-containing cells in germaria characteristic for adult females expressing Cul2GD8913 RNAi under the control of the Scer\GAL4ptc.PU can be partially rescued by combination with either dpphr92, dpphr56 or tkv7 in a heterozygous state or by knocking-down dpp by co-expression of either dppJF01090 or dppJF01091 RNAi.
When present in the genetic background, heterozygous tkv7 reduces the synaptic overgrowth in Rab81/Rab8B229 mutants. When present in the genetic background, trans-heterozygous tkv7/tkvk16713 reduces the synaptic overgrowth in Rab81/Rab8B229 mutants.
One copy of tkv7 completely suppresses the increase in total bouton number seen in S6KL140 mutants.
tkv7/+ significantly suppresses synaptic overgrowth of Acsl05847/AcslKO mutants.
The neuromuscular junction overgrowth phenotype (increased total number of boutons and increased number of satellite boutons) of brat11/brat2L-192-9 larvae is not suppressed by tkv7/+.
One copy of tkv7 does not enhance the small eye phenotype seen when Caf1-105NIG.12892R is expressed under the control of Scer\GAL4ey.PH.
One copy of tkv7 enhances the wing defects seen when Irk2DN.Scer\UAS is expressed under the control of Scer\GAL4Bx-MS1096. Total wing defects in females expressing the P{UAS-Irk2.DN}5.2 insertion line are increased from 46% to 72% of wings. Total wing defects in females expressing the stronger P{UAS-Irk2.DN}5.1 line are increased from 94% to 99% of wings. One copy of tkv7 does not enhance the wing vein defects seen when Irk2Scer\UAS.cDa is expressed under the control of Scer\GAL4Bx-MS1096.
The aberrant wing vein phenotype seen in DdG0269/Y animals is suppressed by tkv7/+.
The glial overmigration phenotype seen in spin10403 eye discs is significantly suppressed if the animals are also heterozygous for tkv7. The glial overmigration phenotype seen in spin10403 eye discs is significantly suppressed if the animals are also carrying tkv7/saxKG07525 or tkv1/tkv7.
The defects in dorsal closure seen in homozygous tkv7 embryos are partially suppressed by expression of AckScer\UAS.cSa under the control of either Scer\GAL4c381 or Scer\GAL4hs.PB. tkv7/+ ; Ack10b/Ack10b embryos show dorsal closure defects at a relatively low frequency, but which is significantly higher than the frequency seen in either tkv7/+ or Ack10b/Ack10b single mutant embryos.
spictmut neuromuscular junction overgrowth phenotypes are fully suppressed in tkv7/tkvk16713 mutants. The synaptic undergrowth phenotypes in larvae homozygous for spictmut in a tkv7/tkvk16713 background are indistinguishable from that of tkv7/tkvk16713 mutants alone. In addition, a heterozygous tkv7 background partially suppresses the neuromuscular junction expansion of spictmut larvae.
Expression of gbbScer\UAS.cKa, under the control of Scer\GAL4A9, in a tkv7/+ background suppresses the extra wing vein phenotype of Scer\GAL4A9>gbbScer\UAS.cKa flies. Around 80% of Scer\GAL4A9>gbbScer\UAS.cKa; tkv7/+ wings show a very mild vein phenotype, while around 20% appear wild type. A tkv7/+ background strongly suppresses the wing phenotype of Scer\GAL4A9>@dppScer\UAS.cHa @ flies so that almost 50% of wings appear wild type.
The mutant dorsal appendage phenotype seen in bwk151/bwk08482 eggs is not significantly modified by tkv7/+.
One copy of tkv7 suppresses the increased bouton number seen at the neuromuscular junction in spin10403/spink09905 animals. spin10403/spink09905 animals which are also mutant for tkv7/tkvk16713 show a further decrease in bouton number.
The tkv7/tkv427 wing phenotype is significantly enhanced by a single copy of pucE69 or hepCA, and in the case of hepCA a wide region of the wing blade is lost, leaving only the base of the wing. The appearance of the apoptotic cluster in the wing discs of tkv7/tkv427 larvae is more apparent if the larvae are also heterozygous for pucE69. The appearance of the cluster of apoptotic cells is suppressed in hepr75/Y ; tkv7/tkv427 ; pucE69/+ larvae.
Eggs laid by Mef2424/tkv7 females show 48.3% wild type dorsal appendages, 11.6% reduced dorsal appendages, 21.5% broad dorsal appendages and short egg length, 2.9% broad dorsal appendages and normal egg length, 12.2% abnormal dorsal appendages with short egg length and 1.2% cup-like eggs with no dorsal appendages and cup-like shape.
The dorsal closure phenotypes of tkv7 embryos are rescued by Cdc42V12.Scer\UAS expressed under the control of Scer\GAL4hs.PB.
Does not suppress the ability of Src42ASu(phl)1-1 to suppress the lethality of phl1/Y flies.
42% of the eggs produced by tkv7/sax1 females have reduced dorsal appendages and 31% are short with fused appendages. The opercula are shifted towards the anterior pole, and appear to lack normal follicle cell imprint patterns.
tkv4, tkv5, tkv7, tkv427 and Df(2L)tkv2 fail to complement dpp short vein alleles for the wing vein defect.
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Fails to complement
Rescued by
Comments
tkvUbi-p63E.PB rescues the inviable mutant phenotype of tkv7/tkv8.
Allelism with tkv indicated by location, failure to complement lethal and viable tkv alleles and thick vein and abnormal thorax phenotype over Tp(2;3)tkv (Szidonya and Reuter, 1988).
Images (0)
Mutant
Wild-type
Stocks (2)
Notes on Origin
Discoverer
Nusslein-Volhard.
Comments
Comments
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (10)
References (68)