FlyBase Allele Report: Dmel\Tl[10b]

General Information

Symbol

Dmel\Tl^10b

Species

D. melanogaster

Name

FlyBase ID

FBal0016833

Feature type

allele

Associated gene

Dmel\Tl

Associated Insertion(s)

Carried in Construct

Also Known As

Toll^10b, Tl^D, Toll10b

Key Links

Genomic Maps

JBrowse

Allele class

gain of function allele

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

gain of function allele

(Bergmann et al., 1996, Lemaitre et al., 1996, Lemaitre et al., 1995, Huang et al., 1993, Whalen and Steward, 1993, Ray et al., 1991, Schneider et al., 1991)

Mutagen

ethyl methanesulfonate

(Erdelyi and Szabad, 1989)

Progenitor genotype

Cytology

Description

Amino acid replacement: C781Y. Residue is located on the external surface near the transmembrane spanning domain.

(Huang et al., 1997)

Amino acid replacement. Mutation is in the extracellular domain.

(Norris and Manley, 1996)

Amino acid replacement.

(Lemaitre et al., 1995)

Nucleotide substitution: G2916A. Amino acid replacement: C781Y. This mutation is in the extracellular domain of the protein.

(Schneider et al., 1991)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

3R:26,840,195..26,840,195 [+]

Nucleotide change:

G26840195A

Amino acid change:

C781Y | Tl-PB; C781Y | Tl-PC; C781Y | Tl-PD

Reported amino acid change:

C781Y

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change. Mutated residue is located on the external surface near the transmembrane spanning domain.

(Huang et al., 1997, Schneider et al., 1991)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

embryo

(Artero et al., 2001)

embryonic/larval cuticle

(Artero et al., 2003)

embryonic/larval hemocyte

(Remillieux-Leschelle et al., 2002)

embryonic/larval hemocyte | increased number

(Huang et al., 2005)

embryonic/larval hemocyte | increased number | larval stage

(Bazzi et al., 2023)

embryonic/larval hemolymph

(Qiu et al., 1998)

embryonic/larval lymph gland

(Davidson et al., 2005)

embryonic/larval plasmatocyte

(Remillieux-Leschelle et al., 2002)

embryonic/larval plasmatocyte | increased number

(Huang et al., 2005)

hemocyte | larval stage

(Qiu et al., 1998)

lamellocyte

(Remillieux-Leschelle et al., 2002, Lanot et al., 2001)

lamellocyte | ectopic

(Davidson et al., 2005)

lamellocyte | increased number

(Huang et al., 2005)

lamellocyte | increased number | larval stage

(Bazzi et al., 2023)

melanotic mass

(Minakhina and Steward, 2006, Braun et al., 1998)

mesoderm

(Schneider et al., 1991)

mesoderm | maternal effect

(Hecht and Anderson, 1993)

primary lobe of embryonic/larval lymph gland | absent | larval stage

(Bazzi et al., 2023)

secondary lobe of embryonic/larval lymph gland | absent | larval stage

(Bazzi et al., 2023)

Detailed Description

Statement

Reference

Heterozygous third instar larvae have melanotic nodules found in the hemocoel or in association with He-positive lymph glands.

(Minakhina and Steward, 2006)

Mutant larvae show abnormal lamellocyte differentiation in the secondary lymph gland lobes, resulting in a dramatic enlargement of the secondary lymph gland lobes.

(Davidson et al., 2005)

The number of hemocytes in the hemolymph of Tl⁸ mutant larvae is significantly higher than in wild type and the number of lamellocytes is increased within the hemocyte pool. Most aggregated hemocytes consist of lamellocytes that are partially to fully melanized. Mitotic analyses of hemocytes show that lamellocytes do not divide in circulation so the excess number must be due to proliferation in the lymph gland. However, plasmatocytes and prohemocytes do divide in circulation in Tl⁸ mutants, while no such division occurs in wild type.

(Huang et al., 2005)

Tl⁸ mutant flies exhibit increased susceptibility to DXV virus infection compared to wild-type. These flies also demonstrate early onset of anoxia sensitivity, while exhibiting a viral load approximately 50% that of wild-type flies at the same time point.

(Zambon et al., 2005)

The cuticle is completely absent in mutant larvae.

(Artero et al., 2003)

Mutant females generate ventralised embryos.

(Kambris et al., 2003)

The concentration of circulating hemocytes in heterozygous larvae is increased compared to controls. The fraction of podocytes and lamellocytes is increased compared to controls.

(Remillieux-Leschelle et al., 2002)

Mutant embryos differentiate almost exclusively as somatic mesoderm.

(Artero et al., 2001)

Lamellocytes appear in circulation during the second instar stage in mutants and are abundant in the hemolymph of third instar larvae (5-25%), where they participate in the formation of melanotic tumours. The crystal cell population is not affected. Lamellocytes are not present in the lymph glands of third instar mutant larvae.

(Lanot et al., 2001)

Heterozygous larvae contain free-floating melanotic masses. These masses are surrounded by several layers of lamellocytes.

(Braun et al., 1998)

Less than 1% of heterozygous adults have melanotic capsules. Hemocyte density in the hemolymph of Tl⁸/+ larvae is significantly higher than in the hemolymph of control larvae. The number of dividing hemocytes in the hemolymph is significantly higher than in wild-type.

(Qiu et al., 1998)

In the absence of immune challenge the antifungal gene Drs is constitutively expressed.

(Lemaitre et al., 1996)

Abnormal fat body morphology. P{Dipt2.2-lacZ} is not induced in heterozygous larvae.

(Lemaitre et al., 1995)

Cell intercalation in lateralized Tl mutant embryos proceeds normally during germ band extension.

(Irvine and Wieschaus, 1994)

Injecting transcripts from Tl⁸ into dorsalized spz⁴ mutant embryos causes ventralization near the site of injection indicating that Tl acts downstream of spz.

(Morisato and Anderson, 1994)

Additional dl protein in gain of function dorsal group backgrounds shifts the nuclear dl gradient ventrally.

(Govind et al., 1993)

Ventralised embryos, the mesoderm is expanded so little or no epidermis is made.

(Hecht and Anderson, 1993)

Strongly ventralized embryos: blastomeres behave as mesodermal cells. Altered pattern of dpp and zen. Heterozygotes show normal twi and sna expression patterns along dorsoventral axis.

(Ray et al., 1991)

Embryos from heterozygous females are extremely ventralised; the mesoderm is expanded. Females carrying Tl⁸ in trans with a null allele of Tl produce ventralised embryos.

(Schneider et al., 1991)

Embryos derived from Tl⁸ females are variably ventralised. At the late cellular blastoderm stage, all the cells have flattened apical surfaces and the nuclei have moved inwards towards the centre of the embryo. Incompletely ventralised embryos show abnormalities in ventral furrow formation, with the furrow generally being larger than in wild-type.

(Leptin and Grunewald, 1990)

Female viability and male fertility are not reduced. Embryos exhibit ventralised phenotype.

(Erdelyi and Szabad, 1989)

dominant

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Tl^10b has melanotic mass phenotype | larval stage | dominant phenotype, enhanceable by gcm^JF01075/Scer\GAL4^srp.Hemo

(Bazzi et al., 2023)

Tl^10b has melanotic mass phenotype | larval stage | dominant phenotype, enhanceable by Df(2L)132

(Bazzi et al., 2023)

Tl^10b has melanotic mass phenotype | larval stage | dominant phenotype, enhanceable by gcm^JF01075/Scer\GAL4^gcm-rA87.P

(Bazzi et al., 2023)

Tl^10b has increased cell number | larval stage | dominant phenotype, enhanceable by gcm^JF01075/Scer\GAL4^gcm-rA87.P

(Bazzi et al., 2023)

Tl^10b has melanotic mass phenotype | larval stage | dominant phenotype, enhanceable by gcm²⁶

(Bazzi et al., 2023)

Tl^10b has increased cell number | larval stage | dominant phenotype, enhanceable by gcm²⁶

(Bazzi et al., 2023)

Suppressed by

Statement

Reference

Scer\GAL4^gcm-rA87.P, Tl^10b, gcm^JF01075 has melanotic mass phenotype | larval stage phenotype, suppressible by gcm^UAS.cBa/Scer\GAL4^gcm-rA87.P

(Bazzi et al., 2023)

Tl^10b has abnormal size | third instar larval stage phenotype, suppressible by Myb^MH107

(Davidson et al., 2005)

Tl^10b has increased cell number | larval stage phenotype, suppressible by Myb^MH107

(Davidson et al., 2005)

NOT Enhancer of

Statement

Reference

Tl^10b is a non-enhancer of visible phenotype of upd1^GMR.PB

(Bach et al., 2003)

NOT Suppressor of

Statement

Reference

Tl^10b is a non-suppressor of visible phenotype of upd1^GMR.PB

(Bach et al., 2003)

Tl^10b is a non-suppressor of lethal | maternal effect | embryonic stage phenotype of Myd88^c03881

(Kambris et al., 2003)

Other

Statement

Reference

Tl^10b, cact³/cact[+] has melanotic mass phenotype phenotype

(Qiu et al., 1998)

Tl[+]/Tl^10b, cact³ has melanotic mass phenotype phenotype

(Qiu et al., 1998)

Phenotype Manifest In

Enhanced by

Statement

Reference

Tl^10b has embryonic/larval hemocyte | larval stage | increased number phenotype, enhanceable by gcm^JF01075/Scer\GAL4^gcm-rA87.P

(Bazzi et al., 2023)

Tl^10b has embryonic/larval hemocyte | larval stage | increased number phenotype, enhanceable by gcm²⁶

(Bazzi et al., 2023)

Tl^10b has lamellocyte | larval stage | increased number phenotype, enhanceable by gcm²⁶

(Bazzi et al., 2023)

NOT Enhanced by

Statement

Reference

Tl^10b has lamellocyte | larval stage | increased number phenotype, non-enhanceable by gcm^JF01075/Scer\GAL4^gcm-rA87.P

(Bazzi et al., 2023)

Suppressed by

Statement

Reference

Tl^10b has lamellocyte phenotype, suppressible by Stat92E[+]/Stat92E⁰⁶³⁴⁶

(Remillieux-Leschelle et al., 2002)

Tl^10b has embryonic/larval plasmatocyte phenotype, suppressible by Stat92E[+]/Stat92E⁰⁶³⁴⁶

(Remillieux-Leschelle et al., 2002)

NOT suppressed by

Statement

Reference

Tl^10b has lamellocyte | increased number | larval stage phenotype, non-suppressible by gcm^JF01075/Scer\GAL4^gcm-rA87.P

(Bazzi et al., 2023)

Tl^10b has lamellocyte phenotype, non-suppressible by brm[+]/brm²

(Remillieux-Leschelle et al., 2002)

Tl^10b has embryonic/larval hemocyte phenotype, non-suppressible by brm[+]/brm²

(Remillieux-Leschelle et al., 2002)

Tl^10b has embryonic/larval plasmatocyte phenotype, non-suppressible by brm[+]/brm²

(Remillieux-Leschelle et al., 2002)

Tl^10b has embryonic/larval hemocyte phenotype, non-suppressible by Stat92E[+]/Stat92E⁰⁶³⁴⁶

(Remillieux-Leschelle et al., 2002)

NOT Enhancer of

Statement

Reference

Tl^10b is a non-enhancer of eye phenotype of upd1^GMR.PB

(Bach et al., 2003)

NOT Suppressor of

Statement

Reference

Tl^10b is a non-suppressor of eye phenotype of upd1^GMR.PB

(Bach et al., 2003)

Tl^10b is a non-suppressor of ventral denticle belt phenotype of Myd88^c03881

(Kambris et al., 2003)

Tl^10b is a non-suppressor of filzkorper phenotype of Myd88^c03881

(Kambris et al., 2003)

Other

Statement

Reference

Tl^10b, cact³/cact[+] has melanotic mass phenotype

(Qiu et al., 1998)

Tl[+]/Tl^10b, cact³ has melanotic mass phenotype

(Qiu et al., 1998)

Additional Comments

Genetic Interactions

Statement

Reference

Abnormal differentiation of lamellocytes is not seen in the secondary lymph gland lobes of Tl⁸ Myb^MH107 double mutant larvae. The lymph gland hemocytes fail to overproliferate in the double mutant larvae.

(Davidson et al., 2005)

The increased concentration of circulating hemocytes seen in Tl⁸/+ larvae is not suppressed by Stat92E⁰⁶³⁴⁶/+, but the increased numbers of podocytes and lamellocytes seen in Tl⁸/+ larvae are strongly suppressed by Stat92E⁰⁶³⁴⁶/+. The concentration of circulating hemocytes in mxc^G43/Y ; Tl⁸/+ larvae is less than the number seen in either single mutant.

(Remillieux-Leschelle et al., 2002)

Tl⁸/+ ; dom^k08108 double mutant larvae contain melanotic masses, although their frequency is markedly reduced compared to Tl⁸/+ single mutant larvae and the masses are devoid of hemocytes.

(Braun et al., 1998)

50.9% of cact³/+ ; Tl⁸/+ adults have melanotic capsules.

(Qiu et al., 1998)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Comments

Tl⁸ maps between e and ca.

(Erdelyi and Szabad, 1989)

Class I allele.

(Schneider et al., 1991)

Class I Tl allele.

(Hecht and Anderson, 1993)

Constitutively active receptor, so dl undergoes nuclear translocation along the entire dorsoventral circumference.

(Gillespie and Wasserman, 1994)

Constitutively active protein.

(Norris and Manley, 1996)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (11)

Reported As

Symbol Synonym

Tl^10B

(Gomez et al., 2024, Yamamoto-Hino et al., 2015, Tevy et al., 2014, Shen and Tanda, 2007, Biemar et al., 2005, Bartoszewski et al., 2004, Bach et al., 2003, Leulier et al., 2003, Vidal et al., 2001, Hader et al., 2000, Hedengren et al., 2000, Manfruelli et al., 1999, Ferrandon et al., 1998, Yagi and Hayashi, 1997, Hacker et al., 1995, Gillespie and Wasserman, 1994, Leptin and Grunewald, 1990)

Tl^10b

(Broderick et al., 2012, Chen et al., 2010, Gordon et al., 2008, Garver et al., 2006, Mulinari et al., 2006, Castillejo-Lopez and Häcker, 2005, Chiu et al., 2005, Ganguly et al., 2005, Huang et al., 2005, Moussian and Roth, 2005, Zambon et al., 2005, Gong et al., 2004, Hedengren-Olcott et al., 2004, Sorrentino et al., 2004, Evans et al., 2003, Badenhorst et al., 2002, Boutros et al., 2002, De Gregorio et al., 2002, Remillieux-Leschelle et al., 2002, Michel et al., 2001, Leulier et al., 2000, Rutschmann et al., 2000, Drier et al., 1999, Kopp et al., 1999, Levashina et al., 1999, Levashina et al., 1998, Nicolas et al., 1998, Qiu et al., 1998, Huang et al., 1997, Lemaitre et al., 1997, Liu et al., 1997, Zhou et al., 1997, Bergmann et al., 1996, Casal and Leptin, 1996, Lemaitre et al., 1996, Norris and Manley, 1996, Reach et al., 1996, Lemaitre et al., 1995, Winans and Hashimoto, 1995, Großhans et al., 1994, Irvine and Wieschaus, 1994, Morisato and Anderson, 1994, Chasan and Anderson, 1993, Costa et al., 1993, Govind et al., 1993, Hecht and Anderson, 1993, Huang et al., 1993, Whalen and Steward, 1993, Winick et al., 1993, Norris and Manley, 1992, Nusslein-Volhard, 1991, Ray et al., 1991, Schneider et al., 1991, Thisse et al., 1991, Erdelyi and Szabad, 1989)

Tl⁸

Tl^D

(Levashina et al., 1999, Manfruelli et al., 1999, Lemaitre et al., 1996)

Toll 10b

(Bachtel et al., 2018)

Toll10^b

(Uv et al., 2000)

Toll10B

(Agaisse and Perrimon, 2004, Bronner and Jackle, 1996)

Toll10b

(Kleino et al., 2005, Meng et al., 1998)

Toll^10B

(Gomez et al., 2024, Hunt et al., 2024, Sandmann et al., 2007, Minakhina and Steward, 2006, Zimmermann et al., 2006, Kambris et al., 2003, Furlong et al., 2001, Creton et al., 2000, Lagueux et al., 2000, Braun et al., 1998, Ferrandon et al., 1997, Hoffmann and Reichhart, 1997, Kubota et al., 1995)

toll^10B

(Farge, 2003)

Name Synonyms

Toll10B

(Biemar et al., 2006)

Toll10b

(Hultmark and Szabad, 2014.5.19, Senger et al., 2006)

Secondary FlyBase IDs

References (123)

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