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General Information
Symbol
Dmel\Tlr3
Species
D. melanogaster
Name
FlyBase ID
FBal0016838
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
Tlr632, Tl632, Tollr632
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Polytene chromosomes normal.
Nature of the lesion
Statement
Reference
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 1 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference
Tlr3/Tlr2 mutant third instar larvae have more eve-positive neurons in the ventral nerve cord compared to wild-type controls.
Tlr3/Tlrv1 flies do not exhibit significantly different survival rates in response to Staphylococcus aureus infection, as compared to control flies.
Tlr3/Tlrv1 flies infected with Pythium insidiosum show significantly lower survival rates than wild-type. As evidenced by histopathological studies, injected P. insidiosum zoospores germinate rapidly in fly hemolymph to form hyphae that subsequently disseminate and invade the thorax, abdomen and head of the mutants. No histopathological alterations are found in wild-type flies after P. insidiosum infection.
Tlr3/Tlrv1 flies show a reduced survival rate compared to control flies after infection with either S. aureus or A. fumigatus.
Infection with the S. aureus 'ItaS' mutant causes earlier death than infection with its parent RN4220 strain in Tlrv1/Tlr3 flies, similar to the findings with wild type flies.
Injection of Tlr3 mutant fruit flies, with either Rhizopus or Mucor spp. results in a higher mortality rate at 48 hours (95%) when compared with injection of wild-type fruit flies. Infection of Tlr3 mutant fruit flies, with C. bertholletaie causes hyperacute mortality (as in wild-type).
Tlr3/Tlrv1 mutant males survive well 10 days post-infection with E.coli, with an 85% survival rate, compared to 96% in wounded controls. Females also survived equally well. Tlr3/Tlrv1 mutant males can withstand B.bassiana fungal infection to a greater level than Tlr3/Tlrv1 mutant females.
Tlr3/Tlr4 embryos display abnormal positioning of cardioblasts and gaps in the dorsal vessel.
Adult females transheterozygous for Tlr3/Tlrv1 display increased sensitivity to infection with various strains of Candida albicans: the survival rate of infected flies is significantly decreased compared to wild-type. Tlr3/Tlrv1 also show significantly higher post-infection fungal load, which unlike in wild-type flies progressively increases with time when infected with a wild-type strain of C. albicans.
The ability of hemizygous larvae to encapsulate L.boulardi eggs is significantly reduced compared to that of control larvae. The ability of Tlr2/Tlr3 larvae to encapsulate L.boulardi eggs is significantly reduced compared to that of control larvae.
The concentration of circulating hemocytes in Tlrv1/Tlr3 larvae is reduced compared to controls.
Shows brain tumours when heterozygous with the MBT chromosome. Shows third larval instar lethality when heterozygous with the MBT chromosome.
Level of Drs induction of bacterially challenged Tlr3/Tlrv1 mutants is lower than in wild type. Pattern of response of CecA1 and CecA2 parallels that of Drs. Dpt and Dro remain fully inducible and pattern of expression of AttA and Def in intermediate. Inducibility of all antimicrobial genes by bacterial challenge in imd1/imd1; Tlr3/Tlrv1 double mutants is severely reduced. Septic injury (pricking with a needle under nonsterile conditions) or infection with E.coli does not noticeably affect Tlr3/Tlrv1 survival, infection with A.fumigatus results in death after 2-3 days clearly associated with uncontrolled fungal development. 40% homozygous double mutant flies survive after septic injury but only a few individuals survive 3 days postinfection with E.coli. Infection of Tlr3/Tlrv1 mutants with A.fumigatus causes 8% survival 3 days postinfection, infection with E.coli causes survival rates similar to wild type.
Dorsalized embryos at the restrictive temperature.
Embryos derived from females are dorsalised.
dppHin embryos derived from dl1 mothers have a weak dorsalizing phenotype: numerous ventral like setae.
temperature-sensitive for the maternal effect, showing stronger dorsalization of the embryonic pattern at 29oC than at 18oC; also temperature-sensitive for viability. TSP for the maternal effect begins slightly before pole cell formation and ends in midsyncytial blastoderm in the offspring of Tlr7 females. TSP for zygotic viability begins late in embryogenesis and extends into the second larval instar in Tlr5 and Tlr6 mutants. recessive
External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhancer of
Statement
Reference
Other
Statement
Reference
Phenotype Manifest In
Suppressor of
Statement
Reference
NOT Suppressor of
Statement
Reference
Tlr3/Tlrv1 is a non-suppressor of lamellocyte phenotype of mxcG43
Tlr3/Tlrv1 is a non-suppressor of podocyte phenotype of mxcG43
Additional Comments
Genetic Interactions
Statement
Reference
The addition of spz4 or Tlrv1/Tlr3 enhances the susceptibility of RelE20 mutants to E.coli and M.luteus infection.
The increased concentration of circulating hemocytes seen in mxcG43 larvae is suppressed by Tlrv1/Tlr3, but the numbers of podocytes and lamellocytes are still increased in the double mutants compared to wild type.
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Fails to complement
Comments
Images (0)
Mutant
Wild-type
Stocks (2)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (10)
References (41)