Allele Dmel\Tl^r3

General Information
Symbol	Dmel\Tl^r3	Species	D. melanogaster
Name		FlyBase ID	FBal0016838
Feature type	allele	Associated gene	Dmel\Tl
Also Known As	Tl^r632, Tl⁶³², Toll^r632

Allele class	hypomorphic allele - genetic evidence
Mutagen	ethyl methanesulfonate
Recent Updates
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FB2013_03
FB2013_02
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Nature of the Allele
Allele class	hypomorphic allele - genetic evidence (Tearle and Nusslein-Volhard, 1987)
Mutagen	ethyl methanesulfonate (Anderson et al., 1985, Tearle and Nusslein-Volhard, 1987)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference
Cytology	Polytene chromosomes normal. (Anderson et al., 1985)
Phenotypic Data
Phenotypic Class
	immune response defective (Chamilos et al., 2008) immune response defective (with Tl^rv1) (Taylor and Kimbrell, 2007, Chen et al., 2010) lethal \| embryonic stage \| maternal effect (Anderson et al., 1985) lethal \| embryonic stage \| maternal effect \| recessive (Cook, 2000.10.9) lethal \| maternal effect \| recessive \| heat sensitive (Gerttula et al., 1988) visible \| heat sensitive (Lemaitre et al., 1996)
Phenotype Manifest In
	cardioblast (with Tl^r4) (Wang et al., 2005) embryo \| dorsal ventral gradient (with Tl^rK344) (Cook, 2000.10.9) embryonic/larval hemocyte (with Tl^rv1) (Remillieux-Leschelle et al., 2002) embryonic dorsal vessel (with Tl^r4) (Wang et al., 2005)
Detailed Description
	Statement Reference Tl[r3]/Tl[rv1] flies show a reduced survival rate compared to control flies after infection with either S. aureus or A. fumigatus. (Chen et al., 2010) Infection with the S. aureus 'ItaS' mutant causes earlier death than infection with its parent RN4220 strain in Tl[rv1]/Tl[r3] flies, similar to the findings with wild type flies. (Hashimoto et al., 2009) Injection of Tl[r3] mutant fruit flies, with either Rhizopus or Mucor spp. results in a higher mortality rate at 48 hours (95%) when compared with injection of wild-type fruit flies. Infection of Tl[r3] mutant fruit flies, with C. bertholletaie causes hyperacute mortality (as in wild-type). (Chamilos et al., 2008) Tl^r3/Tl^rv1 mutant males survive well 10 days post-infection with E.coli, with an 85% survival rate, compared to 96% in wounded controls. Females also survived equally well. Tl^r3/Tl^rv1 mutant males can withstand B.bassiana fungal infection to a greater level than Tl^r3/Tl^rv1 mutant females. (Taylor and Kimbrell, 2007) Tl[r3]/Tl[r4] embryos display abnormal positioning of cardioblasts and gaps in the dorsal vessel. (Wang et al., 2005) The ability of hemizygous larvae to encapsulate L.boulardi eggs is significantly reduced compared to that of control larvae. The ability of Tl^r2/Tl^r3 larvae to encapsulate L.boulardi eggs is significantly reduced compared to that of control larvae. (Sorrentino et al., 2004) The concentration of circulating hemocytes in Tl^rv1/Tl^r3 larvae is reduced compared to controls. (Remillieux-Leschelle et al., 2002) Shows brain tumours when heterozygous with the MBT chromosome. Shows third larval instar lethality when heterozygous with the MBT chromosome. (Riede, 2001) Level of Drs induction of bacterially challenged Tl^r3/Tl^rv1 mutants is lower than in wild type. Pattern of response of CecA1 and CecA2 parallels that of Drs. Dpt and Dro remain fully inducible and pattern of expression of AttA and Def in intermediate. Inducibility of all antimicrobial genes by bacterial challenge in imd¹/imd¹; Tl^r3/Tl^rv1 double mutants is severely reduced. Septic injury (pricking with a needle under nonsterile conditions) or infection with E.coli does not noticeably affect Tl^r3/Tl^rv1 survival, infection with A.fumigatus results in death after 2-3 days clearly associated with uncontrolled fungal development. 40% homozygous double mutant flies survive after septic injury but only a few individuals survive 3 days postinfection with E.coli. Infection of Tl^r3/Tl^rv1 mutants with A.fumigatus causes 8% survival 3 days postinfection, infection with E.coli causes survival rates similar to wild type. (Lemaitre et al., 1996) Dorsalized embryos at the restrictive temperature. (Gerttula et al., 1988) Embryos derived from females are dorsalised. (Carroll et al., 1987) dpp^Hin embryos derived from dl¹ mothers have a weak dorsalizing phenotype: numerous ventral like setae. (Irish and Gelbart, 1987) temperature-sensitive for the maternal effect, showing stronger dorsalization of the embryonic pattern at 29^oC than at 18^oC; also temperature-sensitive for viability. TSP for the maternal effect begins slightly before pole cell formation and ends in midsyncytial blastoderm in the offspring of Tl^r7 females. TSP for zygotic viability begins late in embryogenesis and extends into the second larval instar in Tl^r5 and Tl^r6 mutants. recessive
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhancer of
Statement Reference Tl^r3/Df(3R)ro80b is an enhancer of increased cell death phenotype of spz² (Zhu et al., 2008) Tl^r3/Tl^rv1 is an enhancer of immune response defective phenotype of Rel^E20 (De Gregorio et al., 2002)
Other
Statement Reference Tl^r3, srn⁸⁸ has lethal \| pupal stage phenotype (Riede, 2001, Riede, 2001)
Phenotype Manifest In
Suppressor of
Statement Reference Tl^r3/Tl^rv1 is a suppressor of embryonic/larval hemocyte phenotype of mxc^G43 (Remillieux-Leschelle et al., 2002)
NOT Suppressor of
Statement Reference Tl^r3/Tl^rv1 is a non-suppressor of lamellocyte phenotype of mxc^G43 (Remillieux-Leschelle et al., 2002) Tl^r3/Tl^rv1 is a non-suppressor of podocyte phenotype of mxc^G43 (Remillieux-Leschelle et al., 2002)
Additional Comments
Genetic Interactions
Statement Reference The addition of spz⁴ or Tl^rv1/Tl^r3 enhances the susceptibility of Rel^E20 mutants to E.coli and M.luteus infection. (De Gregorio et al., 2002) The increased concentration of circulating hemocytes seen in mxc^G43 larvae is suppressed by Tl^rv1/Tl^r3, but the numbers of podocytes and lamellocytes are still increased in the double mutants compared to wild type. (Remillieux-Leschelle et al., 2002)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Fails to complement	Tl^rK344 (Cook, 2000.10.9)
Comments
Stocks ( 2 )
Bloomington	3238 Tl^r3 ca¹/TM3, Sb¹
Kyoto	106934 Tl^r3 ca¹/TM3, Sb¹
Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 9 )
Reported As
Symbol Synonym	Tl³ (Akhouayri et al., 2011) Tl⁶³² (Manfruelli et al., 1999, Levashina et al., 1998, Ferrandon et al., 1998, Taylor and Kimbrell, 2007) Tlⁱ⁶³² (Peng et al., 2005) Tl^r3 (Taylor and Kimbrell, 2007, Zhu et al., 2008, Wang et al., 2005, Hashimoto et al., 2009) Tl^r632-5 (Irish and Gelbart, 1987) Tl^r632 (Sorrentino et al., 2004, Remillieux-Leschelle et al., 2002, De Gregorio et al., 2002, Hedengren et al., 2000, Levashina et al., 1999, Manfruelli et al., 1999, Levashina et al., 1998, Nicolas et al., 1998, Lemaitre et al., 1997, Lemaitre et al., 1996, Lemaitre et al., 1995, Roth et al., 1991, Gerttula et al., 1988, Carroll et al., 1987, Tearle and Nusslein-Volhard, 1987, Anderson et al., 1985, Chen et al., 2010, Buchon et al., 2009, Chamilos et al., 2009, Chamilos et al., 2008, Chen et al., 2010) Tlr⁶³² (Riede, 2001) Toll^r632 (Uttenweiler-Joseph et al., 1998) Toll^R632 (Brun et al., 2006)
Name Synonym
Secondary FlyBase IDs

References ( 34 )

Generate a list of	All references relating to this allele ( 34 )

List References by type	Research paper ( 31 ) Supplementary material ( 1 ) Personal communication to FlyBase ( 1 ) Stock list ( 1 )
Recent research papers ( 1 )
	Akhouayri et al., 2011, PLoS Pathog. 7(10): e1002319 Toll-8/tollo negatively regulates antimicrobial response in the Drosophila respiratory epithelium. [FBrf0216452] Show all research papers ...

Allele Dmel\Tlr3

Allele Dmel\Tl^r3