FlyBase Allele Report: Dmel\Tl[r3]

General Information

Symbol

Dmel\Tl^r3

Species

D. melanogaster

Name

FlyBase ID

FBal0016838

Feature type

allele

Associated gene

Dmel\Tl

Associated Insertion(s)

Carried in Construct

Also Known As

Tl^r632, Tl⁶³², Toll^r632

Allele class

hypomorphic allele - genetic evidence

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

hypomorphic allele - genetic evidence

(Tearle and Nusslein-Volhard, 1987)

Mutagen

ethyl methanesulfonate

(Tearle and Nusslein-Volhard, 1987, Anderson et al., 1985)

Mutations Mapped to the Genome

Type

Location

Additional Notes

References

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Progenitor genotype

Cytology

Polytene chromosomes normal.

(Anderson et al., 1985)

Nature of the lesion

Statement

Reference

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of primary immunodeficiency disease

CEA

(Zanette et al., 2013)

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

abdomen | conditional (with Tl^rv1)

(Zanette et al., 2013)

cardioblast (with Tl^r4)

(Wang et al., 2005)

embryo | dorsal ventral gradient (with Tl^rK344)

(Cook, 2000.10.9)

embryonic/larval hemocyte (with Tl^rv1)

(Remillieux-Leschelle et al., 2002)

embryonic dorsal vessel (with Tl^r4)

(Wang et al., 2005)

head | conditional (with Tl^rv1)

(Zanette et al., 2013)

hemolymph | conditional (with Tl^rv1)

(Zanette et al., 2013)

neuron | increased number | third instar larval stage (with Tl^r2)

(Foldi et al., 2017)

thorax | conditional (with Tl^rv1)

(Zanette et al., 2013)

ventral nerve cord | third instar larval stage (with Tl^r2)

(Foldi et al., 2017)

Detailed Description

Statement

Reference

Tl^r3/Tl^r2 mutant third instar larvae have more eve-positive neurons in the ventral nerve cord compared to wild-type controls.

(Foldi et al., 2017)

Tl^r3/Tl^rv1 flies do not exhibit significantly different survival rates in response to Staphylococcus aureus infection, as compared to control flies.

(Ben-Ami et al., 2013)

Tl^r3/Tl^rv1 flies infected with Pythium insidiosum show significantly lower survival rates than wild-type. As evidenced by histopathological studies, injected P. insidiosum zoospores germinate rapidly in fly hemolymph to form hyphae that subsequently disseminate and invade the thorax, abdomen and head of the mutants. No histopathological alterations are found in wild-type flies after P. insidiosum infection.

(Zanette et al., 2013)

Tl^r3/Tl^rv1 flies show a reduced survival rate compared to control flies after infection with either S. aureus or A. fumigatus.

(Chen et al., 2010)

Infection with the S. aureus 'ItaS' mutant causes earlier death than infection with its parent RN4220 strain in Tl^rv1/Tl^r3 flies, similar to the findings with wild type flies.

(Hashimoto et al., 2009)

Injection of Tl^r3 mutant fruit flies, with either Rhizopus or Mucor spp. results in a higher mortality rate at 48 hours (95%) when compared with injection of wild-type fruit flies.

Infection of Tl^r3 mutant fruit flies, with C. bertholletaie causes hyperacute mortality (as in wild-type).

(Chamilos et al., 2008)

Tl^r3/Tl^rv1 mutant males survive well 10 days post-infection with E.coli, with an 85% survival rate, compared to 96% in wounded controls. Females also survived equally well.

Tl^r3/Tl^rv1 mutant males can withstand B.bassiana fungal infection to a greater level than Tl^r3/Tl^rv1 mutant females.

(Taylor and Kimbrell, 2007)

Tl^r3/Tl^r4 embryos display abnormal positioning of cardioblasts and gaps in the dorsal vessel.

(Wang et al., 2005)

Adult females transheterozygous for Tl^r3/Tl^rv1 display increased sensitivity to infection with various strains of Candida albicans: the survival rate of infected flies is significantly decreased compared to wild-type. Tl^r3/Tl^rv1 also show significantly higher post-infection fungal load, which unlike in wild-type flies progressively increases with time when infected with a wild-type strain of C. albicans.

(Alarco et al., 2004)

The ability of hemizygous larvae to encapsulate L.boulardi eggs is significantly reduced compared to that of control larvae. The ability of Tl^r2/Tl^r3 larvae to encapsulate L.boulardi eggs is significantly reduced compared to that of control larvae.

(Sorrentino et al., 2004)

The concentration of circulating hemocytes in Tl^rv1/Tl^r3 larvae is reduced compared to controls.

(Remillieux-Leschelle et al., 2002)

Shows brain tumours when heterozygous with the MBT chromosome. Shows third larval instar lethality when heterozygous with the MBT chromosome.

(Riede, 2001)

Level of Drs induction of bacterially challenged Tl^r3/Tl^rv1 mutants is lower than in wild type. Pattern of response of CecA1 and CecA2 parallels that of Drs. Dpt and Dro remain fully inducible and pattern of expression of AttA and Def in intermediate. Inducibility of all antimicrobial genes by bacterial challenge in imd¹/imd¹; Tl^r3/Tl^rv1 double mutants is severely reduced. Septic injury (pricking with a needle under nonsterile conditions) or infection with E.coli does not noticeably affect Tl^r3/Tl^rv1 survival, infection with A.fumigatus results in death after 2-3 days clearly associated with uncontrolled fungal development. 40% homozygous double mutant flies survive after septic injury but only a few individuals survive 3 days postinfection with E.coli. Infection of Tl^r3/Tl^rv1 mutants with A.fumigatus causes 8% survival 3 days postinfection, infection with E.coli causes survival rates similar to wild type.

(Lemaitre et al., 1996)

Dorsalized embryos at the restrictive temperature.

(Gerttula et al., 1988)

Embryos derived from females are dorsalised.

(Carroll et al., 1987)

dpp^Hin embryos derived from dl¹ mothers have a weak dorsalizing phenotype: numerous ventral like setae.

(Irish and Gelbart, 1987)

temperature-sensitive for the maternal effect, showing stronger dorsalization of the embryonic pattern at 29^oC than at 18^oC; also temperature-sensitive for viability. TSP for the maternal effect begins slightly before pole cell formation and ends in midsyncytial blastoderm in the offspring of Tl^r7 females. TSP for zygotic viability begins late in embryogenesis and extends into the second larval instar in Tl^r5 and Tl^r6 mutants. recessive

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

Tl^r3/Df(3R)ro80b is an enhancer of increased cell death phenotype of spz²

(Zhu et al., 2008)

Tl^r3/Tl^rv1 is an enhancer of abnormal immune response phenotype of Rel^E20

(De Gregorio et al., 2002)

Other

Statement

Reference

Tl^r3, srn⁸⁸ has lethal | pupal stage phenotype

(Riede, 2001)

Phenotype Manifest In

Suppressor of

Statement

Reference

Tl^r3/Tl^rv1 is a suppressor of embryonic/larval hemocyte phenotype of mxc^G43

(Remillieux-Leschelle et al., 2002)

NOT Suppressor of

Statement

Reference

Tl^r3/Tl^rv1 is a non-suppressor of lamellocyte phenotype of mxc^G43

(Remillieux-Leschelle et al., 2002)

Tl^r3/Tl^rv1 is a non-suppressor of podocyte phenotype of mxc^G43

(Remillieux-Leschelle et al., 2002)

Additional Comments

Genetic Interactions

Statement

Reference

The addition of spz⁴ or Tl^rv1/Tl^r3 enhances the susceptibility of Rel^E20 mutants to E.coli and M.luteus infection.

(De Gregorio et al., 2002)

The increased concentration of circulating hemocytes seen in mxc^G43 larvae is suppressed by Tl^rv1/Tl^r3, but the numbers of podocytes and lamellocytes are still increased in the double mutants compared to wild type.

(Remillieux-Leschelle et al., 2002)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

Tl^rK344

(Cook, 2000.10.9)

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

3238

106934

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (10)

Reported As

Symbol Synonym

Tl³

(Akhouayri et al., 2011)

Tl⁶³²

(Taylor and Kimbrell, 2007, Manfruelli et al., 1999, Ferrandon et al., 1998, Levashina et al., 1998)

Tlⁱ⁶³²

(Peng et al., 2005)

Tl^r3

(Araki et al., 2019, Alpar et al., 2018, Del Signore et al., 2017, Liu et al., 2016, Hashimoto et al., 2009, Zhu et al., 2008, Taylor and Kimbrell, 2007, Wang et al., 2005)

Tl^r632-5

(Irish and Gelbart, 1987)

Tlr⁶³²

(Riede, 2001)

Toll-1^r3

(Foldi et al., 2017)

Toll^R632

(Brun et al., 2006)

Toll^r632

(Uttenweiler-Joseph et al., 1998)

Name Synonyms

Secondary FlyBase IDs

References (43)

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