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General Information
Symbol
Dmel\Tlr4
Species
D. melanogaster
Name
FlyBase ID
FBal0016839
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
Tollrm9, Tlrm9, Tollrm10, Tlrm10, mel(3)9, mel(3)10
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
point mutation
Nucleotide change:
AAA26838799AGC
Reported nucleotide change:
AA1639GC
Amino acid change:
K355S | Tl-PB; K355S | Tl-PC; K355S | Tl-PD
Reported amino acid change:
K355S
Comment:
The reported Lys to Ser amino acid change (AAA to AGC) requires nucleotide changes at two positions of the codon. This is one of two amino acid substitutions in Tl in this strain. The site of the nucleotide substitution in the mutant was inferred by FlyBase based on the reported amino acid change.
point mutation
Nucleotide change:
C26839994A
Reported nucleotide change:
C2715A
Amino acid change:
T714N | Tl-PB; T714N | Tl-PC; T714N | Tl-PD
Reported amino acid change:
T714N
Comment:
One of two amino acid substitutions in this strain.
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Polytene chromosomes normal.
Nature of the lesion
Statement
Reference
Nucleotide substitution: AA1639GC. Amino acid replacement: K355S. Nucleotide substitution: C2715A. Amino acid replacement: T714N. These mutations are in the extracellular domain of the protein.
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference
Tlr3/Tlr4 embryos display abnormal positioning of cardioblasts and gaps in the dorsal vessel.
The entire dorso-ventral axis forms neuroectoderm in homozygous Tlr4 embryos.
Cell intercalation in lateralized Tl mutant embryos proceeds normally during germ band extension.
Unique lateralized pattern. Cuticular phenotype of embryos resemble that of Toll-dominant embryos, but denticles finer and less heavily pigmented and body shape often an elongated tube. Recessive.
Dorsalized phenotype facilitates removal of perivitelline fluid.
Lateralized embryos: cuticle is an elongated tube covered with rings of denticles. dpp, zen and twi are only expressed at the poles, only transient expression of sna is seen.
Embryos from homozygous females are lateralised.
Females produced lateralized embryos.
External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference
The variable dorsalized embryonic phenotype of wekEX14 mutants is enhanced by Tlr4. While 51% of wekEX14 embryos show an extreme dorsalized phenotype, this penetrance is increased to 97% in wekEX14; Tlr4/+ embryos.
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Fails to complement
Comments
Images (0)
Mutant
Wild-type
Stocks (2)
Notes on Origin
Discoverer
Comments
Comments
It is not known whether one or both amino acid replacements generate the mutant phenotype. Phenotypic and molecular analysis suggests that the allele previously termed "Tlrm10" is the same mutagenic event as "Tlrm9".
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (10)
References (31)