Allele Dmel\tld¹⁴

General Information
Symbol	Dmel\tld¹⁴	Species	D. melanogaster
Name		FlyBase ID	FBal0016885
Feature type	allele	Associated gene	Dmel\tld
Also Known As	tld^10E, tld^10E95

Map ( GBrowse )
Allele class	antimorphic allele - genetic evidence, loss of function allele
Mutagen	ethyl methanesulfonate
Recent Updates
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FB2013_03
FB2013_02
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Nature of the Allele
Allele class	antimorphic allele - genetic evidence (Arora and Nusslein-Volhard, 1992, Ferguson and Anderson, 1992, Childs and O'Connor, 1994, Finelli et al., 1994, Raftery et al., 1995, Marques et al., 1997) loss of function allele (Ray et al., 1991)
Mutagen	ethyl methanesulfonate (Tearle and Nusslein-Volhard, 1987)
Mutations Mapped to the Genome

Type Location Additional Notes References point mutation 3R:20,576,635..20,576,635 comment=Position of mutation on reference sequence inferred by FlyBase curator. Difference beteween actual and reported amino acid substitution due to authors using M11 as the start Met. evidence=experimental na_change=C20576635T pr_change=S286F\|tld-PA reported_na_change=C827T reported_pr_change=S276F (Childs and O'Connor, 1994) point mutation 3R:20,576,635..20,576,635 comment=Position of mutation on reference sequence inferred by FlyBase curator. Difference beteween actual and reported amino acid substitution due to authors using M11 as the start Met. evidence=experimental na_change=C20576635T pr_change=S286F\|tld-PA reported_na_change=C?T reported_pr_change=S276F (Finelli et al., 1994)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Amino acid replacement: S276F. Nucleotide substitution: C827T. Mutation within the protease domain. (Childs and O'Connor, 1994) Amino acid replacement: S276F. Amino acid 276 falls in the protease domain of the tld protein. (Finelli et al., 1994)
Cytology
Phenotypic Data
Phenotypic Class
	lethal (with tld¹) (Ferguson and Anderson, 1992) lethal \| embryonic stage \| recessive (Finelli et al., 1994)
Phenotype Manifest In
	embryonic/larval dorsal vessel (Yin and Frasch, 1998) embryonic epidermis (Finelli et al., 1994) visceral mesoderm (Yin and Frasch, 1998)
Detailed Description
	Statement Reference Neuromuscular junctions appear normal in structure and localisation in tld⁹/tld¹⁴ third instar larvae. (Meyer and Aberle, 2006) Homozygous embryos form some residual visceral mesoderm. The dorsal vessel is missing, except for a few residual cells. (Yin and Frasch, 1998) Antagonistic activity towards dpp. (Childs and O'Connor, 1994) Strong ventralization. Dominantly enhances dpp phenotype. (Finelli et al., 1994) Moderate ventralised phenotype. Defective movements of the germ band: due to loss of the amnioserosa and because the dorsalmost cells have acquired the lateral fate of the dorsal ectoderm. Dorsal cell fates are deleted and ventrolateral mitotic domains are expanded. (Arora and Nusslein-Volhard, 1992) Ventralized embryos: rings or patches of ventral denticles along dorsoventral axis. Disruption of germ band extension that leads to the invagination of posterior segments into the interior of the embryo. (Ray et al., 1991) strong
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
Statement Reference tld¹/tld¹⁴ has lethal phenotype, suppressible by sog^Y506 (Ferguson and Anderson, 1992)
Phenotype Manifest In
Enhancer of
Statement Reference tld¹⁴ is an enhancer of phenotype of dpp⁹⁷ (Raftery et al., 1995) tld¹⁴ is an enhancer of phenotype of dpp⁹⁸ (Raftery et al., 1995) tld¹⁴ is an enhancer of phenotype of dpp⁹⁹ (Raftery et al., 1995) tld¹⁴ is an enhancer of phenotype of dpp¹⁰⁰ (Raftery et al., 1995) tld¹⁴ is an enhancer of phenotype of dpp¹⁰¹ (Raftery et al., 1995) tld¹⁴ is an enhancer of phenotype of dpp¹⁰² (Raftery et al., 1995) tld¹⁴ is an enhancer of phenotype of dpp¹⁰³ (Raftery et al., 1995) tld¹⁴ is an enhancer of phenotype of dpp^d-ho (Raftery et al., 1995) tld¹⁴ is an enhancer of phenotype of dpp^e90 (Raftery et al., 1995) tld¹⁴ is an enhancer of phenotype of dpp^hr4 (Raftery et al., 1995) tld¹⁴ is an enhancer of phenotype of dpp^hr27 (Raftery et al., 1995) tld¹⁴ is an enhancer of phenotype of dpp^hr89 (Raftery et al., 1995) tld¹⁴ is an enhancer of phenotype of dpp^hr92 (Raftery et al., 1995) tld¹⁴ is an enhancer of phenotype of dpp^hr93 (Raftery et al., 1995)
Additional Comments
Genetic Interactions
Statement Reference Strong enhancer of all dpp alleles, except dpp^e87 and dpp^hr56. (Raftery et al., 1995)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Fails to complement	tld^MBT (Riede, 2001)
Comments
Stocks ( 1 )
Bloomington	34041 st¹ e¹ tld¹⁴/TM3, Sb¹
Notes on Origin
Discoverer

Comments
	Strong tld allele. Allelic series for tld antagonistic effect on dpp: tld^B3 > tld¹⁴ = tld⁶ > tld¹ > tld^6P41 > tld^9Q19. (Childs and O'Connor, 1994)
External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 4 )
Reported As
Symbol Synonym	10E95 (Riede, 2001) tld^10E95 (Marques et al., 1997, Childs and O'Connor, 1994, Meyer and Aberle, 2006) tld^10E (Yin and Frasch, 1998, Belvin et al., 1995, Finelli et al., 1994, Ferguson and Anderson, 1992, Arora and Nusslein-Volhard, 1992, Ray et al., 1991, Tearle and Nusslein-Volhard, 1987, ) tld¹⁴
Name Synonym
Secondary FlyBase IDs

References ( 12 )
Research paper	Meyer and Aberle, 2006, Development 133(20): 4035--4044 At the next stop sign turn right: the metalloprotease Tolloid-related 1 controls defasciculation of motor axons in Drosophila. [FBrf0193012] Riede, 2001, D. I. S. 84: 103--114 Gene combinations inducing neoplasms in Drosophila. [FBrf0150723] Yin and Frasch, 1998, Dev. Genet. 22(3): 187--200 Regulation and function of tinman during dorsal mesoderm induction and heart specification in Drosophila. [FBrf0102646] Marques et al., 1997, Cell 91(3): 417--426 Production of a DPP activity gradient in the early Drosophila embryo through the opposing actions of the SOG and TLD proteins. [FBrf0099382] Belvin et al., 1995, Genes Dev. 9(7): 783--793 Cactus protein degradation mediates Drosophila dorsal-ventral signaling. [FBrf0079887] Raftery et al., 1995, Genetics 139(1): 241--254 Genetic screens to identify elements of the decapentaplegic signaling pathway in Drosophila. [FBrf0079237] Childs and O'Connor, 1994, Dev. Biol. 162(1): 209--220 Two domains of the tolloid protein contribute to its unusual genetic interaction with decapentaplegic. [FBrf0068447] Finelli et al., 1994, Development 120(4): 861--870 Mutational analysis of the Drosophila tolloid gene, a human BMP-1 homolog. [FBrf0073067] Arora and Nusslein-Volhard, 1992, Development 114: 1003--1024 Altered mitotic domains reveal fate map changes in Drosophila embryos mutant for zygotic dorsoventral patterning genes. [FBrf0055835] Ferguson and Anderson, 1992, Development 114: 583--597 Localized enhancement and repression of the activity of the TGF- family member, decapentaplegic, is necessary for dorsal-ventral pattern formation in the Drosophila embryo. [FBrf0055881] Ray et al., 1991, Development 113(1): 35--54 The control of cell fate along the dorsal-ventral axis of the Drosophila embryo. [FBrf0053782]
Stock list	Tearle and Nusslein-Volhard, 1987, D. I. S. 66: 209--269 Tubingen mutants and stock list. [FBrf0045941]

Allele Dmel\tld14

Allele Dmel\tld¹⁴