Allele Dmel\trc¹

General Information
Symbol	Dmel\trc¹	Species	D. melanogaster
Name		FlyBase ID	FBal0017031
Feature type	allele	Associated gene	Dmel\trc

Map ( GBrowse )
Allele class	loss of function allele
Mutagen	ethyl methanesulfonate
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class	loss of function allele (Emoto et al., 2006)
Mutagen	ethyl methanesulfonate
Mutations Mapped to the Genome

Type Location Additional Notes References point mutation 3L:19,825,441..19,825,441 comment=Position of mutation on reference sequence inferred by FlyBase curator. Difference in amino acid position due to slightly different predicted cds used by authors. evidence=experimental na_change=G19825441C pr_change=R391P\|trc-PA reported_pr_change=R395P (Geng et al., 2000)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Amino acid replacement: R395P. (Geng et al., 2000)
Cytology
Phenotypic Data
Phenotypic Class
	developmental rate defective \| pupal stage (with trc⁸) (Fei et al., 2002) neuroanatomy defective \| third instar larval stage (with Df(3L)BSC445) (Han et al., 2012) planar polarity defective \| somatic clone (Lee and Adler, 2002) visible \| somatic clone (with trc⁸) (Geng et al., 2000)
Phenotype Manifest In
	antenna (with trc⁸) (Geng et al., 2000) arista lateral (with trc⁸) (Fei et al., 2002) dendrite \| cell autonomous \| somatic clone \| supernumerary (Emoto et al., 2004) denticle (with Df(3L)kto2) (Geng et al., 2000) denticle belt (with Df(3L)kto2) (Geng et al., 2000) dorsal multidendritic neuron ddaC \| third instar larval stage (with Df(3L)BSC445) (Han et al., 2012) egg \| somatic clone (Horne-Badovinac et al., 2012) multidendritic neuron & dendrite (Emoto et al., 2004) multidendritic neuron & dendrite \| somatic clone (Emoto et al., 2004) multidendritic neuron & dendrite \| supernumerary (Emoto et al., 2004) wing hair \| somatic clone (Lee and Adler, 2002) wing hair \| somatic clone \| supernumerary (with trc⁸) (Geng et al., 2000)
Detailed Description
	Statement Reference trc[1]/Df(3L)BSC445 mutant third instar larvae show a significant increase in the proportion of dorsal midline ddaC dendrite length that is enclosed within the epidermis rather than attached to the ECM. The amount of enclosed dendrite seen in each heterozygote is similar to wild type. Most of the dendritic crossings in these mutants are between enclosed dendrites and dendrites attached to the ECM and are thus non-contacting. (Han et al., 2012) Females carrying homozygous follicle cell clones can produce round eggs. (Horne-Badovinac et al., 2012) trc[1]/+ heterozygotes exhibit wild-type dendritic tiling, with wild-type levels of dendritic crossing points per υm[2]. (Emoto et al., 2006) trc¹ mutants exhibit supernumerary terminal branching and defective dendritic tiling. Mutant larvae show an increase in terminal branch number, and the branches of ddaC neurons often overlap each other. This tiling defect can be seen independently from the overbranching defect. The crossing branches have rigid and straight trajectories. When somatic clones are made in the neurons, mutant ddaE, IdaA and ddaC dendrites display a 50% increase in the number of branches. Mutant clones also exhibit a tiling defect. In mutants, the v'ada and vdaB dendrites often invade neighbouring fields. Major branches as well as terminal branches overlap extensively. (Emoto et al., 2004) trc¹/trc⁸ mutant show a variable delay in developmental rate. trc¹/trc⁸ mutant pupae can have multiply split laterals on the aristae. Laterals are seen to split at a wide range of developmental stages. As development proceeds, the distance from the proximal base of the lateral to the proximal most branch-point increases, as does the distance between the proximal and distal branch-points. An increase in the length of the arms distal to branch-points is also seen. (Fei et al., 2002) trc¹/trc⁸ clones in the wing produce a weak multiple hair cell phenotype. Some hairs are split distally. The hairs appear clustered close together. In some cases the hairs are oriented almost orthogonally to the plane of the wing. trc¹/Df(3L)kto2 larvae have an abnormal pattern of denticles, with the wild-type fairly precise rows of denticles being replaced by a more chaotic arrangement. At the level of the individual denticle, the predominant abnormality is splitting (more than 30% of denticles in some denticle bands show this phenotype). trc¹/trc⁸ flies routinely show branching of one or more of the lateral extensions of the antenna. (Geng et al., 2000) Mutant wing and notum have rosettes of three or more short trichomes instead of single long hairs as in wild type. Useful as cell marker. Mutant cell autonomous in mitotic cells in a wild-type background, but not in a Minute background (Vinson and Adler, 1987, D. I. S. 66).
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Other
Statement Reference fry²/fry[+], mats^e235, trc¹/trc[+] has visible \| dominant phenotype (He et al., 2005) fry²/fry[+], mats^e03077, trc¹/trc[+] has visible \| dominant phenotype (He et al., 2005) hpo^KC202, trc¹/trc[+] has neuroanatomy defective phenotype (Emoto et al., 2006) hpo^KC202/hpo[+], trc¹ has neuroanatomy defective phenotype (Emoto et al., 2006) hpo^MGH4, trc¹/trc[+] has neuroanatomy defective phenotype (Emoto et al., 2006) hpo^MGH4/hpo[+], trc¹ has neuroanatomy defective phenotype (Emoto et al., 2006) rictor^Δ2, trc¹/trc[+] has neuroanatomy defective phenotype (Koike-Kumagai et al., 2009) Sin1^e03756, trc¹/trc[+] has neuroanatomy defective phenotype (Koike-Kumagai et al., 2009) stan[+]/stan^E59, trc¹ has neuroanatomy defective \| third instar larval stage phenotype (Matsubara et al., 2011) Tor^ΔP, trc¹/trc[+] has neuroanatomy defective phenotype (Koike-Kumagai et al., 2009)
Phenotype Manifest In
Enhancer of
Statement Reference trc¹ is an enhancer of wing hair phenotype of ultA¹ (Lee and Adler, 2002)
Other
Statement Reference fry²/fry[+], mats^e235, trc¹/trc[+] has wing hair \| supernumerary phenotype (He et al., 2005) fry²/fry[+], mats^e03077, trc¹/trc[+] has wing hair \| supernumerary phenotype (He et al., 2005) hpo^KC202, trc¹/trc[+] has multidendritic dendrite phenotype (Emoto et al., 2006) hpo^KC202/hpo[+], trc¹ has multidendritic dendrite phenotype (Emoto et al., 2006) hpo^MGH4, trc¹/trc[+] has multidendritic dendrite phenotype (Emoto et al., 2006) hpo^MGH4/hpo[+], trc¹ has multidendritic dendrite phenotype (Emoto et al., 2006) rictor^Δ2, trc¹/trc[+] has class IV dendritic arborizing neuron phenotype (Koike-Kumagai et al., 2009) rictor^Δ2, trc¹/trc[+] has dendrite phenotype (Koike-Kumagai et al., 2009) Sin1^e03756, trc¹/trc[+] has class IV dendritic arborizing neuron phenotype (Koike-Kumagai et al., 2009) Sin1^e03756, trc¹/trc[+] has dendrite phenotype (Koike-Kumagai et al., 2009) stan[+]/stan^E59, trc¹ has dendrite \| third instar larval stage phenotype (Matsubara et al., 2011) stan[+]/stan^E59, trc¹ has dorsal multidendritic neuron ddaC \| third instar larval stage phenotype (Matsubara et al., 2011) Tor^ΔP, trc¹/trc[+] has class IV dendritic arborizing neuron phenotype (Koike-Kumagai et al., 2009) Tor^ΔP, trc¹/trc[+] has dendrite phenotype (Koike-Kumagai et al., 2009)
Additional Comments
Genetic Interactions
Statement Reference stan[E59]/trc[1] double heterozygous larvae show increased crossing of dendrites in the dendritic arbor of the ddaC class IV neurons compared to wild type (neither single heterozygote shows this phenotype). (Matsubara et al., 2011) Class IV da neurons of Sin1[e03756]/+ ; trc[1]/+, rictor[Δ2]/+ ; trc[1]/+ and Tor[ΔP]/+ ; trc[1]/+ double heterozygous larvae show defects in dendritic tiling, with a significantly higher number of dendritic branches crossing one another compared to wild type. (Koike-Kumagai et al., 2009) trc[1]/+ tutl[23]/+ double heterozygotes do not show any enhanced class I da neuron branching defects or class IV neuron dendrite self-avoidance defects compared to tutl[23]/+ single heterozygotes. (Long et al., 2009) Transheterozygotes for trc[1]/hpo[MGH4] exhibit obvious iso-neuronal as well as hetero-neuronal tiling defects, including a significant increase in dendritic crossing-points compared to single mutants. Transheterozygotes for wts[x1]/trc[1] do not show any significant dendritic phenotypes. Transheterozygotes for trc[1]/hpo[KC202] exhibit obvious iso-neuronal as well as hetero-neuronal tiling defects, including a significant increase in dendritic crossing-points compared to single mutants. (Emoto et al., 2006) fry² trc¹ mats^e235 triple heterozygotes have a weak multiple wing hair phenotype in 48% of wings. (He et al., 2005) fry¹/trc¹ larvae exhibit tiling defects, though they have normal dendritic length and branch points. (Emoto et al., 2004)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Rescued by	trc¹ is rescued by trc^{Scer\UAS.T:Zzzz\FLAG}/Scer\GAL4⁴⁷⁷ (Emoto et al., 2004)
Not rescued by	trc¹ is not rescued by trc^{K122A.Scer\UAS.T:Zzzz\FLAG}/Scer\GAL4⁴⁷⁷/trc^{K122A.Scer\UAS.T:Zzzz\FLAG} (Emoto et al., 2004) trc¹ is not rescued by trc^{S292A.T449A.Scer\UAS.T:Zzzz\FLAG}/Scer\GAL4⁴⁷⁷ (Emoto et al., 2004)
Comments
Stocks ( 4 )
Bloomington	1135 ru¹ jv¹ trc¹ ca¹/TM3, Ser¹ 5261 y¹; trc¹ P{neoFRT}80B kar² ry⁵⁰⁶/TM6C, ry^CB Sb¹ Tb¹
Kyoto	106218 ru¹ jv¹ trc¹ ca¹/TM3, Ser¹ 108130 y¹; trc¹ P{neoFRT}80B kar² ry⁵⁰⁶/TM6C, ry^CB Sb¹ Tb¹
Notes on Origin
Discoverer	A. Ferrus.

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 1 )
Reported As
Symbol Synonym	trc¹ (Fei et al., 2002, He et al., 2005, Horne-Badovinac et al., 2012, Emoto et al., 2006, Long et al., 2009, Koike-Kumagai et al., 2009, Matsubara et al., 2011, Han et al., 2012)
Name Synonym
Secondary FlyBase IDs

References ( 13 )
Research paper	Han et al., 2012, Neuron 73(1): 64--78 Integrins Regulate Repulsion-Mediated Dendritic Patterning of Drosophila Sensory Neurons by Restricting Dendrites in a 2D Space. [FBrf0217239] Horne-Badovinac et al., 2012, G3 (Bethesda) 2(3): 371--378 A screen for round egg mutants in Drosophila identifies tricornered, furry, and misshapen as regulators of egg chamber elongation. [FBrf0217749] Matsubara et al., 2011, Genes Dev. 25(18): 1982--1996 The seven-pass transmembrane cadherin Flamingo controls dendritic self-avoidance via its binding to a LIM domain protein, Espinas, in Drosophila sensory neurons. [FBrf0215818] Koike-Kumagai et al., 2009, EMBO J. 28(24): 3879--3892 The target of rapamycin complex 2 controls dendritic tiling of Drosophila sensory neurons through the Tricornered kinase signalling pathway. [FBrf0209531] Long et al., 2009, Development 136(20): 3475--3484 Dendrite branching and self-avoidance are controlled by Turtle, a conserved IgSF protein in Drosophila. [FBrf0208798] Emoto et al., 2006, Nature 443(7108): 210--213 The tumour suppressor Hippo acts with the NDR kinases in dendritic tiling and maintenance. [FBrf0195286] He et al., 2005, Mol. Biol. Cell 16(9): 4139--4152 Drosophila mob family proteins interact with the related tricornered (trc) and warts (wts) kinases. [FBrf0188034] Emoto et al., 2004, Cell 119(2): 245--256 Control of dendritic branching and tiling by the Tricornered-kinase/Furry signaling pathway in Drosophila sensory neurons. [FBrf0180024] Fei et al., 2002, J. Cell Sci. 115(19): 3797--3806 The growth of Drosophila bristles and laterals is not restricted to the tip or base. [FBrf0152120] Lee and Adler, 2002, Genetics 160(4): 1535--1547 The function of the frizzled pathway in the Drosophila wing is dependent on inturned and fuzzy. [FBrf0147064] Geng et al., 2000, Genetics 156(4): 1817--1828 The tricornered Gene, which is required for the integrity of epidermal cell extensions, encodes the Drosophila nuclear DBF2-related kinase. [FBrf0131297] Dickinson and Thatcher, 1997, Cell Motility Cytoskel. 38(1): 9--21 Morphogenesis of denticles and hairs in Drosophila embryos: involvement of actin-associated proteins that also affect adult structures. [FBrf0098231]
Personal communication to FlyBase	Martin, 1995.12.5, [title not yet available] [title not yet available] [FBrf0084563]

Allele Dmel\trc1

Allele Dmel\trc¹