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General Information
Symbol
Dmel\trh1
Species
D. melanogaster
Name
FlyBase ID
FBal0017036
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
trh5D55
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
point mutation
Nucleotide change:

T371067A

Reported nucleotide change:

T?A

Amino acid change:

I233N | trh-PA; I233N | trh-PB; I270N | trh-PC; I233N | trh-PD; I233N | trh-PE; I270N | trh-PF; I233N | trh-PG

Reported amino acid change:

I233N

Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

Amino acid replacement: I233N.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

trh1 mutant embryos do not exhibit tracheal invagination with no local apical constrictions or cell-shape changes occurring, with the epithelium remaining flat.

in trh1 mutant embryos, there is no specific accumulation of either actin or myosin at the invaginating edge.

In trh1 homozygous embryos, salivary duct cells fail to invaginate, instead remaining clustered on the surface of the embryo.

Homozygous mutant embryos have no tracheal tubules.

Mutants lack tracheal cell identity.

In homozygous embryos the Malpighian tubules empty into a single large sack that is attached to the hindgut and no ureters are evident (in contrast to wild-type embryos where the two anterior tubules join in a common ureter and the two posterior tubules join in a common ureter, so that two ureters empty into the hindgut). The sack could result from the malformation of either the proximal tubules or the ureters. The more distal segments of the tubules, although tubular, fail to elongate or undergo cell rearrangement, resulting in tubules that are less than 1/2 the length and wider than normal tubules. The hindguts are normal in shape but somewhat shorter than normal.

The filzkorper do not elongate and trachea do not form properly. The oesophagus, gastric caecae and Malpighian tubules are unaffected.

Tracheal structures are not formed in the embryo. Only small invaginations can occasionally be detected. Salivary ducts do not form and the filzkorper are defective.

Trachea absent. Majority of sensory nerves form normally, though incidence of misrouted axons is significantly increased, elongation of axons is slowed and sensory neurons which fail to send out an axon are frequent. These defects are seen most strongly in trh1, twi1 double mutants where neither trachea nor muscles are present.

embryonic lethal. Tracheae are absent and filzkorper not elongated.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Enhanced by
Statement
Reference

jing[+]/jing01094, trh1 has trachea phenotype, enhanceable by tgo1/tgo[+]

trh1 has trachea phenotype, enhanceable by jing[+]/jing01094

jing01094, trh1/trh[+] has trachea phenotype, enhanceable by tgo1/tgo[+]

NOT Enhancer of
Statement
Reference

trh1 is a non-enhancer of visceral branch phenotype of jing3

trh1 is a non-enhancer of dorsal trunk primordium phenotype of jing3

NOT Suppressor of
Statement
Reference

trh1 is a non-suppressor of visceral branch phenotype of jing3

trh1 is a non-suppressor of dorsal trunk primordium phenotype of jing3

Other
Statement
Reference
Additional Comments
Genetic Interactions
Statement
Reference

trh1, jing01094 double heterozygous embryos display a loss of about half of their trachea. When tgo1 is also added, 69% of embryos display a loss of about half of their trachea. jing01094, tgo1 double heterozygous embryos display a loss of about 1/5 of their trachea. jingrev, trh1 double heterozygotes have a wild-type tracheal system. When btlH82Δ3 is also added to trh1, tgo1 double heterozygous 69% of embryos display a loss of about half of their trachea. The addition of trh1 to jing3 homozygotes has no effect on the jing3 tracheal phenotype.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
Comments
Comments

trh alleles fall into an allelic series. From strongest to weakest: trh1 = trh8 > trh7 > trh10512 > trh6 > trh5 > trh4.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
References (20)