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General Information
Symbol
Dmel\tsl3
Species
D. melanogaster
Name
FlyBase ID
FBal0017197
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
tsl146
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Nucleotide change:

T21778579A

Amino acid change:

Y279N | tsl-PA; Y279N | tsl-PB; Y279N | tsl-PC

Reported amino acid change:

Y279N

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

Amino acid replacement: Y279N.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Late embryos from tsl3/tslΔ transheterozygous mothers exhibit a fully penetrant "terminal class mutant" phenotype, in which some terminal structures are missing (including head structures, abdominal segment 8, telson, and filzkorper), as compared to controls.

tsl3/tsl4 transheterozygotes present a pole-hole phenotype during early embryonic stage and present patterning defects at late embryonic stage, as shown by head and telson defects in cuticle preparations, as compared to controls.

Little or no tll expression is detected in the posterior of syncytial or cellular blastoderm embryos, at the anterior the early tll cap does not appear and an abnormal anterior tll stripe appears by the late syncytial blastoderm.

Embryos derived from homozygous females lack the labrum anteriorly, and the head is reduced in size. Posteriorly, A8 and the telson are deleted.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
NOT suppressed by
Statement
Reference
Suppressor of
Statement
Reference
NOT Suppressor of
Statement
Reference

tsl[+]/tsl3 is a non-suppressor of decreased cell number | recessive | embryonic stage phenotype of gclrev390

Other
Phenotype Manifest In
NOT suppressed by
Statement
Reference

tsl4/tsl3 has embryonic telson phenotype, non-suppressible by gclrev390/gclrev390

tsl4/tsl3 has embryonic head phenotype, non-suppressible by gclrev390/gcl[+]

tsl4/tsl3 has embryonic telson phenotype, non-suppressible by gclrev390/gcl[+]

tsl4/tsl3 has primordial germ cell | embryonic stage phenotype, non-suppressible by gclrev390/gcl[+]

tsl4/tsl3 has embryonic head phenotype, non-suppressible by gclrev390/gclrev390

Suppressor of
Statement
Reference
NOT Suppressor of
Statement
Reference

tsl[+]/tsl3 is a non-suppressor of germline cell | embryonic stage phenotype of gclrev390

Other
Additional Comments
Genetic Interactions
Statement
Reference

The pole-hole phenotype in early embryonic stage and the patterning defects in late embryonic stage (i.e. head and telson defects in cuticle preparations) displayed by tsl3/tsl4 transheterozygotes are not suppressed by gclrev390 heterozygosity or homozygosity.

The decreased primordial germ cells in gclrev390 homozygous early embryos (i.e. mitotic cycles 12/13) is fully suppressed by tsl3/tsl4 transheterozygosity, but not by tsl3 heterozygosity. tsl3/tsl4, gclrev390/+ and tsl3/tsl4, gclrev390/gclrev390 double mutants do not present any obvious defects in either centrosome positioning or cell division of primordial germ cells in the early embryo, as compared to controls.

Anisotropic epithelial constriction still occurs in bcd6 nosL7 tsl3 triple mutant embryos that lack anterior-posterior polarity.

Anterior-posterior polarization of cells in the extending germband is disrupted in embryos from bcd6 nosL7 tsl3 homozygous mothers.

Embryos derived from bcd6 nosL7 tsl3 triple mutant females have uniform yolk stalk diameters (in contrast to wild type where there are three domains of varying yolk stalk diameter along the anterior-posterior axis of the embryo). The shallow cellularisation front of the anterior domain and the greater depth of the pre-cephalic furrow domain are also lost. Other aspects of cellularisation are normal in these embryos. Nuclear spacing is uniform along the anterior-posterior axis in embryos derived from bcd6 nosL7 tsl3 triple mutant females (in contrast to wild type where there is an anterior domain of lower nuclear density). The larger diameter of the actin caps seen in the anterior of the wild-type embryo in cycle 11 and 12 is not seen in embryos derived from bcd6 nosL7 tsl3 triple mutant females.

bcd6 nosL7 tsl3 embryos develop a cuticle but no segmental pattern. Injection of wild type posterior pole plasm induces formation of anterior abdominal segments towards the end of the embryo.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
Comments
Comments

A strong allele of tsl.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (4)
References (15)