Y279N | tsl-PA; Y279N | tsl-PB; Y279N | tsl-PC
Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.
The pole-hole phenotype in early embryonic stage and the patterning defects in late embryonic stage (i.e. head and telson defects in cuticle preparations) displayed by tsl3/tsl4 transheterozygotes are not suppressed by gclrev390 heterozygosity or homozygosity.
The decreased primordial germ cells in gclrev390 homozygous early embryos (i.e. mitotic cycles 12/13) is fully suppressed by tsl3/tsl4 transheterozygosity, but not by tsl3 heterozygosity. tsl3/tsl4, gclrev390/+ and tsl3/tsl4, gclrev390/gclrev390 double mutants do not present any obvious defects in either centrosome positioning or cell division of primordial germ cells in the early embryo, as compared to controls.
Embryos derived from bcd6 nosL7 tsl3 triple mutant females have uniform yolk stalk diameters (in contrast to wild type where there are three domains of varying yolk stalk diameter along the anterior-posterior axis of the embryo). The shallow cellularisation front of the anterior domain and the greater depth of the pre-cephalic furrow domain are also lost. Other aspects of cellularisation are normal in these embryos. Nuclear spacing is uniform along the anterior-posterior axis in embryos derived from bcd6 nosL7 tsl3 triple mutant females (in contrast to wild type where there is an anterior domain of lower nuclear density). The larger diameter of the actin caps seen in the anterior of the wild-type embryo in cycle 11 and 12 is not seen in embryos derived from bcd6 nosL7 tsl3 triple mutant females.