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General Information
Symbol
Dmel\tsl4
Species
D. melanogaster
Name
FlyBase ID
FBal0017198
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
tsl691
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Nucleotide change:

C21778626T

Amino acid change:

A263V | tsl-PA; A263V | tsl-PB; A263V | tsl-PC

Reported amino acid change:

A263V

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

Amino acid replacement: A263V.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Late embryos from tsl4/tslΔ transheterozygous mothers exhibit a fully penetrant "terminal class mutant" phenotype, in which some terminal structures are missing (including head structures, abdominal segment 8, telson, and filzkorper), and exhibit a highly penetrant posterior-ventral cuticle hole phenotype, as compared to controls.

tsl4/tsl3 transheterozygotes present a pole-hole phenotype during early embryonic stage and present patterning defects at late embryonic stage, as shown by head and telson defects in cuticle preparations, as compared to controls.

Pupariation is delayed in tsl4/Df(3R)cakiX-313 mutant larvae.

Pupariation is delayed in homozygous tsl4 mutant larvae.

Pupariation is delayed in tsl4/tslX-307 mutant larvae.

Embryos derived from tor12D/+ ; tsl4/tsl4 females have the same phenotype as embryos derived from tor12D/+ females, indicating that tsl functions upstream of tor.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
NOT suppressed by
Statement
Reference
Suppressor of
Statement
Reference
NOT Suppressor of
Statement
Reference

tsl4/tsl[+] is a non-suppressor of decreased cell number | recessive | embryonic stage phenotype of gclrev390

Other
Phenotype Manifest In
Suppressed by
Statement
Reference

tsl4 has filzkorper phenotype, suppressible | partially by Dp(3;3)bicS

tsl4 has embryo | anterior phenotype, suppressible | partially by bcd+t8.7

tsl4 has embryo | anterior phenotype, suppressible | partially by bcd+t8

NOT suppressed by
Statement
Reference

tsl4/tsl3 has primordial germ cell | embryonic stage phenotype, non-suppressible by gclrev390/gcl[+]

tsl4/tsl3 has embryonic head phenotype, non-suppressible by gclrev390/gclrev390

tsl4/tsl3 has embryonic telson phenotype, non-suppressible by gclrev390/gclrev390

tsl4/tsl3 has embryonic head phenotype, non-suppressible by gclrev390/gcl[+]

tsl4/tsl3 has embryonic telson phenotype, non-suppressible by gclrev390/gcl[+]

Suppressor of
Statement
Reference
NOT Suppressor of
Statement
Reference

tsl4/tsl[+] is a non-suppressor of germline cell | embryonic stage phenotype of gclrev390

Other
Additional Comments
Genetic Interactions
Statement
Reference

The pole-hole phenotype in early embryonic stage and the patterning defects in late embryonic stage (i.e. head and telson defects in cuticle preparations) displayed by tsl4/tsl3 transheterozygotes are not suppressed by gclrev390 heterozygosity or homozygosity.

The decreased primordial germ cells in gclrev390 homozygous early embryos (i.e. mitotic cycles 12/13) is fully suppressed by tsl4/tsl3 transheterozygosity, but not by tsl4 heterozygosity. tsl4/tsl3, gclrev390/+ and tsl4/tsl3, gclrev390/gclrev390 double mutants do not present any obvious defects in either centrosome positioning or cell division of primordial germ cells in the early embryo, as compared to controls.

Four copies of the bcd gene (bcd+t8.7 or bcd+t8) are able to rescue some anterior structures in about 40% of tsl4 derived embryos. However not all embryos with rescued labrum and dorsal bridge have a perfectly aligned head skeleton. Occasionally embryos survive long enough to hatch and move around.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (4)
Notes on Origin
Discoverer
Comments
Comments

Clonal analysis indicates that tsl expression is required only in subpopulations of follicle cells located at the poles of the oocyte.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (5)
References (21)