A263V | tsl-PA; A263V | tsl-PB; A263V | tsl-PC
Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.
The pole-hole phenotype in early embryonic stage and the patterning defects in late embryonic stage (i.e. head and telson defects in cuticle preparations) displayed by tsl4/tsl3 transheterozygotes are not suppressed by gclrev390 heterozygosity or homozygosity.
The decreased primordial germ cells in gclrev390 homozygous early embryos (i.e. mitotic cycles 12/13) is fully suppressed by tsl4/tsl3 transheterozygosity, but not by tsl4 heterozygosity. tsl4/tsl3, gclrev390/+ and tsl4/tsl3, gclrev390/gclrev390 double mutants do not present any obvious defects in either centrosome positioning or cell division of primordial germ cells in the early embryo, as compared to controls.
Four copies of the bcd gene (bcd+t8.7 or bcd+t8) are able to rescue some anterior structures in about 40% of tsl4 derived embryos. However not all embryos with rescued labrum and dorsal bridge have a perfectly aligned head skeleton. Occasionally embryos survive long enough to hatch and move around.