FB2025_01 , released February 20, 2025
Allele: Dmel\tub2
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General Information
Symbol
Dmel\tub2
Species
D. melanogaster
Name
FlyBase ID
FBal0017243
Feature type
allele
Associated gene
Dmel\tub
Associated Insertion(s)
Carried in Construct
Also Known As
tub238, tube2
Key Links
Genomic Maps

Allele class

amorphic allele - genetic evidence

Mutagen
Nature of the Allele
Allele class

amorphic allele - genetic evidence

(Ray et al., 1991)
Mutagen
ethyl methanesulfonate
(Hecht and Anderson, 1993)
Progenitor genotype
Cytology
Description

Nucleotide substitution: G418A. Amino acid replacement: E140K.

(Letsou et al., 1993)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
point_mutation
3R:4,388,347..4,388,347 [+]
Nucleotide change:

G4388347A

Reported nucleotide change:

G418A

Amino acid change:

E140K | tub-PC

Reported amino acid change:

E140K

(Letsou et al., 1993)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      tub2/tubR5.6 females produce embryos that are severely dorsalised.

      (Clemmons and Wasserman, 2013)

      The ability of homozygous larvae to encapsulate L.boulardi eggs is significantly reduced compared to that of control larvae.

      (Sorrentino et al., 2004)

      The concentration of circulating hemocytes in homozygous larvae is reduced compared to controls.

      (Remillieux-Leschelle et al., 2002)

      Pupal lethal when heterozygous with the MBT chromosome.

      (Riede, 2001)

      Hemocyte density in the hemolymph of homozygous larvae is significantly lower than in the hemolymph of control larvae.

      (Qiu et al., 1998)

      Level of Drs induction of bacterially challenged tub2/tub1 mutants is lower than in wild type. Pattern of response of CecA1 and CecA2 parallels that of Drs. Dpt and Dro remain fully inducible and pattern of expression of AttA and Def in intermediate. Infection of tub2/tub3 mutants with A.fumigatus causes 3% survival 3 days postinfection, infection with E.coli causes 71% survival 3 days postinfection.

      (Lemaitre et al., 1996)

      Strongly dorsalised embryos. Transheterozygotes with tub1 are completely dorsalised, the phenotype of tub2 Tl3t/tub1 is unchanged from the transheterozygote. Transheterozygotes with tub5 are weakly dorsalised, the phenotype of tub2 Tl3t/tub5 is lateralised. Transheterozygotes with tub4 are moderately dorsalised, the phenotype of tub2 Tl3t/tub1 is unchanged from the transheterozygote.

      (Hecht and Anderson, 1993)

      Injected dl- perivitelline fluid had no effect on embryos.

      (Stein and Nusslein-Volhard, 1992)

      tub1/tub2 heterozygous embryos gastrulate symmetrically and secrete a twisted and hollow tube of dorsal cuticle.

      (Letsou et al., 1991)

      Dorsalized embryos: all cuticle cells along the dorsoventral axis behave like dorsal cells of the wild type embryo. zen expression pattern refines at stage 5, dpp pattern does not refine at all, twi and sna are expressed during late stages of embryogenesis.

      (Ray et al., 1991)

      Embryos derived from homozygous females are dorsalised.

      (Carroll et al., 1987)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Suppressor of
      Statement
      Reference

      tub2 is a suppressor of partially lethal - majority die | recessive phenotype of cact7

      (Qiu et al., 1998)
      Other
      Statement
      Reference

      srn88, tub2 has lethal | pupal stage phenotype

      (Riede, 2001)
      Phenotype Manifest In
      Enhancer of
      Suppressor of
      Statement
      Reference

      tub2/tub2 is a suppressor of embryonic/larval hemocyte phenotype of mxcG43

      (Remillieux-Leschelle et al., 2002)
      NOT Suppressor of
      Statement
      Reference

      tub2/tub2 is a non-suppressor of lamellocyte phenotype of mxcG43

      (Remillieux-Leschelle et al., 2002)

      tub2/tub2 is a non-suppressor of embryonic/larval plasmatocyte phenotype of mxcG43

      (Remillieux-Leschelle et al., 2002)
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      The variable dorsalized embryonic phenotype of wekEX14 mutants is enhanced by tub2. While 51% of wekEX14 embryos show an extreme dorsalized phenotype, this penetrance is increased to 96% in wekEX14; tub2/+ embryos.

      (Chen et al., 2006)

      The increased concentration of circulating hemocytes seen in mxcG43 larvae is suppressed by tub2/tub2, but the numbers of podocytes and lamellocytes are still increased in the double mutants compared to wild type.

      (Remillieux-Leschelle et al., 2002)

      The zygotic semi-lethality of cact7 homozygotes is strongly suppressed by tub2.

      (Qiu et al., 1998)

      Double mutant combinations with eaD1 are strongly dorsalizing.

      (Chasan et al., 1992)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (2)
      Notes on Origin
      Discoverer
      Comments
      Comments

      Same mutation was introduced into a cDNA and used in RNA injection experiments, proving that the lesion is responsible for the tub maternal effect phenotype.

      (Letsou et al., 1993)
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      References (39)