Open Close
General Information
Symbol
Dmel\Ubx9.22
Species
D. melanogaster
Name
FlyBase ID
FBal0017483
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
Ubx9-22, Ubx922
Mutagen
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
deletion
Comment:

Position of mutation on reference sequence inferred by FlyBase curator based on author's statement. See Figure 2 of FBrf0046283. Mutation is a deletion of 1580bp which removes the splice acceptor site and much of the homeobox.

Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Nature of the lesion
Statement
Reference

Deletion and associated frameshift producing a truncated protein that lacks the homeodomain and C-terminal sequences.

Deletion of 1.6kb removing part of intron 3 and the common 3' exon up to and including the first 48 codons of the homeodomain. Inactivates all Ubx isoforms.

Deletion of 16kb within the Ubx transcript.

Deletion in the homeobox.

1.5kb deletion including the homeobox.

Deletion in the Ubx coding region.

1587bp deletion removing the final 1.4kb of the 50kb long intron, the splice acceptor site of the 3' exon and 48 codons of the homeobox. A sequence of 6 nucleotides is inserted between the deletion breakpoints.

Deletion in the 3' Ubx exon.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Ubx1/Ubx9.22 transheterozygous embryos show a significant increase in the proportion of mitotic neuroblast daughter cells, but not mitotic neuroblasts, only in the A1 ventral nerve cord segment, as compared to controls.

The anterior pair of Malpighian tubules do not migrate past the developing midgut in Ubx9.22 mutant embryos.

Homozygous Ubx9.22 mutant embryos fail to develop the 2-3 most anterior pairs of alary muscles.

Ubx9.22 clones within the haltere do not grow larger than their twin spots.

The P compartment of Ubxpbx-1/Ubx9.22 haltere discs is increased in size relative to the disc, such that the P:A size ration is 1.45. This effect is not seen in Ubx9.22/+ discs.

In stage-15 Ubx9.22 mutant embryos the lymph gland is expanded posteriorly into the abdominal segments.

Homozygous embryos frequently show a range of defects in dorsal vessel development, including incomplete dorsal migration of the cardiac precursors and minor morphological defects of the dorsal vessel, but there is still a clear distinction between the heart and the aorta in these animals.

The aorta does not differentiate normally, particularly in the anterior region, in mutant embryos. Pericardiac cells are also disorganised in several positions, particularly in the anterior segments.

When heterozygous with UbxHC71-1.HC166D.Dup.Rec.ry+ the notum reduction phenotype is much more penetrant and severe. These animals also show an enlargement of the haltere, with anterior bristles, characteristic of a weak bx transformation.

Haltere enlarged, average bristle number 0.9 (+-0.9 (SD); N=138).

No gross morphological defects are seen in the central nervous system of Ubx9.22 Abd-BM1 double mutant embryos.

Embryos lack the precursor for ventral muscle 26 in A1 and A2.

Transformation of capitellum to wing.

Elevated levels of ae transcript are found in parasegment 6.

Homozygous embryos have a set of extra leg discs, a rudimentary wing disc and two new sets of spiracles.

The phenotype of fu41 Ubx9.22 double mutants can be interpreted as an addition of the corresponding single mutant phenotypes.

Does not affect the frequency of the trx bithorax-variegated phenotype in heterozygous combination with Df(3R)red-P52.

Transvection positive with Ubxbx-34e.

A2-A4 denticle belts are transformed into thoracic belts, and A5-A8 denticle belts are modified, having much more marked thoracic features in hemizygous embryos.

Homozygotes complete embryogenesis and occasionally survive as third instar larvae. They have the characteristic Ubx phenotype of transformed denticle belts and three pairs of anterior spiracles. In Ubx61d/Ubxbxd-100 larvae A1 is transformed to T3, and a pair of presumptive leg neuromeres is formed in this transformed segment. In Ubx9.22/Ubxbxd-100 adults the commissures of the CNS in parasegment 6 are transformed to resemble those of parasegment 5.

The frequency of variegated bithorax transformations seen in Df(3R)red-P52 heterozygotes is not increased in flies also heterozygous for Ubx9.22.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement
Reference

Ubx9.22 has visible phenotype, enhanceable by Df(2L)PJ17

Phenotype Manifest In
Enhanced by
Statement
Reference

Ubx9.22 has haltere phenotype, enhanceable by Df(2L)PJ17

Ubx9.22 has macrochaeta | ectopic phenotype, enhanceable by Df(2L)PJ17

Ubx9.22 has phenotype, enhanceable by fl(2)d2

Ubx9.22 has phenotype, enhanceable by vir3

Ubx9.22 has haltere sensillum phenotype, enhanceable by crn3

Ubx9.22 has haltere sensillum phenotype, enhanceable by crn4

Ubx9.22 has haltere sensillum phenotype, enhanceable by kz26

Ubx9.22 has haltere sensillum phenotype, enhanceable by Df(4)G

Ubx9.22 has haltere sensillum phenotype, enhanceable by Hrb27C[+]/Hrb27C10280

Suppressed by
Additional Comments
Genetic Interactions
Statement
Reference

Expression of tkvScer\UAS.cNa under the control of Scer\GAL4Act5C in a Ubxpbx-1/Ubx9.22 background reduces the size of the P compartment of haltere discs relative to that observed in Ubxpbx-1/Ubx9.22 discs (from 1.45 to 0.83), although this ratio is still greater than that of wild-type discs.

The addition of Df(2L)PJ17 to Ubx9.22 flies enhances the haltere bristle phenotype seen in Ubx9.22 alone. An average of 3.5 bristles per haltere are seen in Df(2L)PJ17,Ubx9.22 flies, as opposed to none in wild-type and 0.9 per haltere in Ubx9.22 flies alone.

Hrb27C10280 enhances the haltere phenotype of Ubx9.22/+ such that halteres are enlarged, and average bristle number 2.6 (+-1.5 (SD); N=46).

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (7)
Notes on Origin
Discoverer
Comments
Comments

Ubx9.22 homozygous germ line clones are viable and show no maternal effects.

External Crossreferences and Linkouts ( 2 )
Crossreferences
GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
Synonyms and Secondary IDs (4)
References (55)