usp2 mutants die at the first larval molt.
uspQ288A usp2 animals progress normally through to apolysis of the the epidermis away from the cuticle (albeit with a briefly longer 3rd instar feeding stage compared to normal larvae). Subsequently, uspQ288A usp2 animals do not form a normal puparium - it is dorso-ventrally flattened, the partially everted anterior spiracles point directly forward, the cuticle is only faintly sclerotized, the 'puparial' cuticle is transparent rather than brown, and head eversion is incomplete. Most animals stop development during the early pupal stage and eventually die.
uspN325A usp2 animals progress normally through to apolysis of the the epidermis away from the cuticle. Subsequently, uspN325A usp2 animals do not show most of the morphogenetic events of puparium formation: anterior spiracles do not evert to their full extension or proper angle, the cuticle does not become barrel-shaped and instead remains soft and untanned. These mutants then die within a day or two.
uspQ288A.L366A usp2 animals progress normally through to apolysis of the the epidermis away from the cuticle. Subsequently, uspQ288A.L366A usp2 animals do not show most of the morphogenetic events of puparium formation: anterior spiracles do not evert to their full extension or proper angle, the cuticle does not become barrel-shaped and instead remains soft and untanned. These mutants then die within a day or two.
The dendritic arbors of homozygous ddaC dendritic arborisation neuron MARCM clones have a reduced number of branches compared to controls.
Homozygous clones in the margin region of the wing disc show precocious differentiation of neurons. Homozygous clones in the margin region of wing discs cultured in vitro in the absence of 20-hydroxyecdysone undergo neurogenesis and initiate axonal outgrowth, in contrast to wild-type tissue which arrests development under these conditions.
Expression of usphs.PO using a single 30 minute 37oC heat pulse during the first larval instar stage is sufficient to rescue more than 70% of usp2 animals to the third instar stage. These rescued animals fail to wander near the end of the third instar stage, maintain their larval shape, become motionless at the surface of the food, fail to respond to touch stimulus and do not evert their anterior spiracles. After several hours in this abnormal "stationary stage", the animals begin to apolyze from their third instar cuticle, retracting from both the anterior and posterior ends. Apolysis is complete by 24 hours after becoming stationary. A supernumerary cuticle covers the posterior two-thirds of the animal. This cuticle is thick, well infiltrated with tracheae and segmentally ridged along the body. Most of these rescued animals die by 72 hours after the stationary phase. Imaginal discs appear normal in rescued third instar larvae and begin to evert following the stationary phase, but arrest their development at a point normally seen 1 hour after puparium formation. The gastric caecae retract, although this occurs gradually over 24 hours. A slight compaction of the larval midgut is seen, but the larval cells do not die and the adult midgut does not form. The number of midgut imaginal cells does not change significantly in the 24 hours following the stationary phase. Larval salivary gland development is normal until the end of the third instar stage, however destruction of the larval salivary gland does not occur and the gland persists until the animal dies.
Most of the eggs laid by usp2 mosaic females mutant in the germline are fertilised.