|Feature type||allele||Associated gene||Dmel\vap|
|Also Known As||vap1|
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|Nature of the Allele|
|Mutations Mapped to the Genome|
|Associated Sequence Data|
|Nature of the lesion|
Nucleotide substitution: G?A. The point mutation in this allele creates a stop codon. The predicted protein product is truncated just before the GAP catalytic domain.
|Phenotype Manifest In|
autophagic vacuole & neuron & adult brain
lysosome & neuron & adult brain
neuron & adult brain
14 day old vap[KS67] mutant flies show vacuoles in all parts of the brain. These increase dramatically with age
In brain sections of 1-day-old vapKS67 homozygous flies there is no apparent difference in morphology compared with wild type. However, ultrastructural analysis of these brains reveals the accumulation in neurons of autolysosomes and vacuoles containing whorls of membranous material as well as empty vacuoles; all signs of autophagic cell death that are absent in wild-type neurons. Brain sections of seven-day-old vapKS67 homozygous flies show vacuolization in the optic lobe and central brain, and ultrastructural analysis reveals dying neurons with digested cytoplasm, although nuclei remain intact. By 14-days of age, brain sections of these flies have a strong spongiform appearance. Despite signs of autophagic cell death, these neurons do not express markers of cell death, an lack the normal morphological characteristics of apoptotic cells. Glial cells in the brains of these flies remain normal. The maximum lifespan of vapKS67 homozygous flies is 25 days, compared to 90 days for wild-type controls.
Show significantly reduced ability to develop ethanol tolerance.
Flies have a number of brain defects, the exact phenotype depending on the genetic background. In the original genetic background in which it was induced, vapKS67 produces the following phenotype; large vacuoles are restricted to the peduncles, the ellipsoid body is open ventrally and the noduli are misshapen in many flies. When placed in a Canton S background, the peduncle-specific vacuoles are smaller than in the original genetic background, and the brain is more dissociated. The central complex defects are similar to the phenotype in the original genetic background.
Isolated as a neuro-anatomical mutant (Heisenberg and Bohl, 1979). Mutant shows vacuolar spaces at a certain depth along the pedunculi of the mushroom bodies as if extrinsic cells are undergoing degeneration. Intrinsic fibers appear continuous. Viability of mutants reduced (Heisenberg).
|Phenotype Manifest In|
Expression of Appl[s.Scer\UAS.T:Ivir\HA1] under the control of Scer\GAL4[elav-C155] suppresses the vacuolisation seen in vap[KS67] mutant 14 day old males.
|Complementation & Rescue Data|
|Stocks ( 0 )|
|Notes on Origin|
|External Crossreferences & Linkouts|
|Synonyms & Secondary IDs ( 2 )|
|Secondary FlyBase IDs|
|References ( 4 )|