FlyBase Allele Report: Dmel\vas[1]

General Information

Symbol

Dmel\vas¹

Species

D. melanogaster

Name

FlyBase ID

FBal0017845

Feature type

allele

Associated gene

Dmel\vas

Associated Insertion(s)

Carried in Construct

Also Known As

vas^PD, vasa^PD, vas^PD23, vasa^PD23

Allele class

amorphic allele - genetic evidence

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

amorphic allele - genetic evidence

(Schupbach and Wieschaus, 1986)

Mutagen

ethyl methanesulfonate

(Ashburner et al., 1999.10.12, Schupbach and Wieschaus, 1991, Tearle and Nusslein-Volhard, 1987)

Mutations Mapped to the Genome

Type

Location

Additional Notes

References

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Progenitor genotype

Cytology

Nature of the lesion

Statement

Reference

Sequencing revealed that there is no amino acid replacement in the coding sequence.

(Liang et al., 1994)

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Oocytes from vas¹ females express vas protein only at the germarium stage.

(Lasko and Ashburner, 1990)

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Phenotypic Data

Phenotypic Class

female sterile

(Schupbach and Wieschaus, 1991)

female sterile (with vas³)

(Xiol et al., 2014)

grandchildless

(Schupbach and Wieschaus, 1986)

lethal | maternal effect | recessive

(Lehmann and Nusslein-Volhard, 1991)

lethal | non-rescuable maternal effect

(Schupbach and Wieschaus, 1986)

Phenotype Manifest In

Detailed Description

Statement

Reference

Embryos from vas¹ homozygous mothers fail to form pole cells and show impaired dorsal patterning (assessed by no. of ftz stripes).

(Dehghani and Lasko, 2016)

vas¹/vas^PH165 egg chambers are similar to wild-type, consisting of 15 nurse cells and an oocyte surrounded by a layer of follicle cells.

(Liu et al., 2009)

100% of eggs laid by vas¹/vas¹⁵, vas¹/vas⁶ or vas¹/vas⁷ females appear wild-type.

(Tomancak et al., 1998)

Strong abdominal defects.

(Ephrussi et al., 1991)

Absence of posterior pole plasm, polar granules and pole cells.

(Lehmann and Nusslein-Volhard, 1991)

Normal eggs. Embryos lack polar granules, pole cells and abdominal segments.

(Schupbach and Wieschaus, 1991)

Reduced posterior vas localization in the oocyte. vas protein is only detectable at the germarium stage of ovarian development. Females lay wild type numbers of eggs that develop into embryos lacking abdominal segments and pole cells.

(Lasko and Ashburner, 1990)

Homozygous embryos derived from homozygous females have no polar granules, fail to form pole cells and have deletions of abdominal structures. Posterior localization of vas and CycB transcripts is completely abolished.

(Raff et al., 1990)

Homozygous females produce embryos that fail to form pole cells, lack polar granules normally found at the posterior pole, and have deletions of abdominal segments.

(Schupbach and Wieschaus, 1986)

Embryos derived from mutant females completely lack pole cells and show deletions in the abdominal segments. The same phenotype is seen in embryos derived from homozygous or hemizygous females.

(Schupbach and Wieschaus, 1986)

maternal-effect lethal. Embryos from homozygous mothers exhibit a so-called 'grandchildless-knirps' phenotype: all eggs lack polar granules and no pole cells are formed; most of the embryos show large deletions of abdominal segments, whereby anterior parts of segment A1 become fused to posterior parts of segment A8. Telson elements are always present and relatively normal. Eggs have abnormal shape. Analysis of germ-line clones indicates that the mutation is germ-line autonomous (Schupbach and Wieschaus, 1986). Homozygous vasa males cannot be distinguished from wild-type males in viability and fertility.

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Suppressed by

Statement

Reference

vas¹ has primordial germ cell | maternal effect phenotype, suppressible | partially by bel::vas^{vas.C.bel.EGFP}

(Dehghani and Lasko, 2016)

vas¹ has embryo | maternal effect phenotype, suppressible | partially by bel::vas^{vas.C.bel.EGFP}

(Dehghani and Lasko, 2016)

vas¹/vas³ has embryonic abdominal segment phenotype, suppressible | partially by nos^{nos.+2'ME-3X.Tag:HA}

(Bergsten et al., 2001)

vas¹ has embryonic abdominal segment phenotype, suppressible by nos^{αTub84B.UTR.Tag:HA}

(Crucs et al., 2000)

vas¹/vas³ has abdomen phenotype, suppressible by nos^N5

(Wang and Lehmann, 1991)

NOT suppressed by

Statement

Reference

vas¹/vas³ has embryonic abdominal segment phenotype, non-suppressible by nos^nos.+1.+2'

(Bergsten et al., 2001)

vas¹/vas³ has embryonic abdominal segment phenotype, non-suppressible by nos^{nos.+2RO.Tag:HA}

(Bergsten et al., 2001)

vas¹ has phenotype, non-suppressible by nos^{αTub84B.UTR.virTCE.Tag:HA}

(Crucs et al., 2000)

vas¹ has phenotype, non-suppressible by nos^nos.+1.Hsp83

(Crucs et al., 2000)

vas¹ has primordial germ cell phenotype, non-suppressible by osk^+t8

(Smith et al., 1992)

Enhancer of

Statement

Reference

vas[+]/vas¹ is an enhancer of primordial germ cell | maternal effect phenotype of Fsn^UAS.Tag:HA, Scer\GAL4^VP16.nos.UTR

(Kugler et al., 2010)

vas^PH165/vas¹ is an enhancer of oocyte phenotype of mei-P26^fs1/mei-P26¹

(Liu et al., 2009)

vas^PH165/vas¹ is an enhancer of egg chamber phenotype of mei-P26^fs1/mei-P26¹

(Liu et al., 2009)

vas^PH165/vas¹ is an enhancer of nurse cell phenotype of mei-P26^fs1/mei-P26¹

(Liu et al., 2009)

Additional Comments

Genetic Interactions

Statement

Reference

The failure to form pole cells as well as the impaired dorsal patterning (assessed by no. of ftz stripes) observed in embryos from vas¹ homozygous mothers is partially rescued in progeny from vas¹;bel::vas^{vas.C.bel.T:Avic\GFP-EGFP} females.

(Dehghani and Lasko, 2016)

vas¹/+ enhances the primordial germ cell phenotype resulting from the overexpression of Scer\GAL4^{nos.UTR.T:Hsim\VP16}>Fsn^{Scer\UAS.T:Ivir\HA1}.

(Kugler et al., 2010)

More than 50% of vas¹/vas^PH165 ; mei-P26¹/mei-P26^fs1 egg chambers are tumorous and fail to differentiate nurse cells and oocytes. The double mutant egg chambers contain mostly early-stage cystocytes as they contain either punctate spectrosomes or branched fusomes.

(Liu et al., 2009)

nos^{nos.+2'ME-3X.T:Ivir\HA1} partially rescues the abdominal segmentation defect of embryos derived from vas¹/vas³ females; 2% of the embryos develop no abdominal segments, 53% develop 1-3 abdominal segments, 43% develop 4-6 abdominal segments and 2% develop 7-8 abdominal segments. The embryos do not have anterior defects. nos^nos.+1.+2' or nos^{nos.+2RO.T:Ivir\HA1} fail to rescue the abdominal segmentation defect of embryos derived from vas¹/vas³ females; 100% of the embryos develop no abdominal segments. The embryos do not have anterior defects.

(Bergsten et al., 2001)

The lack of abdominal segments seen in embryos derived from vas¹/vas³ females is rescued if the females also carry nos^{αTub84B.UTR.T:Ivir\HA1}, but not if they carry nos^{αTub84B.UTR.virTCE.T:Ivir\HA1} or nos^nos.+1.Hsp83.

(Crucs et al., 2000)

Embryos derived from vas¹/vas³ females carrying nos^nos.+2.Hsp83 or nos^{nos.+2-3X.T:Ivir\HA1} do not produce any abdominal segments. 98% of embryos derived from vas¹/vas³ females carrying nos^{nos.+2'.Hsp83} produce 7-8 abdominal segments and 58% of these embryos show loss of head or thorax structures. Embryos derived from vas¹/vas³ females carrying nos^{nos.+2'-2X.T:Ivir\HA1} develop 0-3 abdominal segments in 16% of cases, 4-6 abdominal segments in 51% of cases and 7-8 abdominal segments in 34% of cases. Embryos derived from vas¹/vas³ females carrying nos^{nos.+2'-3X.T:Ivir\HA1} develop 0-3 abdominal segments in 72% of cases and 4-6 abdominal segments in 28% of cases.

(Bergsten and Gavis, 1999)

vas¹;6xP{osk⁺108} embryos exhibit a vas cuticular phenotype, deletion of most abdominal segments. Embryos also fail to form pole cells.

(Smith et al., 1992)

Does not interact with RpII140^wimp maternal effect.

(Parkhurst and Ish-Horowicz, 1991)

Injection of nos^N5 RNA into vas¹/vas³ embryos completely rescues the abdominal phenotype.

(Wang and Lehmann, 1991)

tor^hb1+hb2 transcripts fully rescue posterior and anterior development in vas¹ embryos.

(Wharton and Struhl, 1991)

In double mutants vas¹, fs(1)N¹² all abdominal segments are transformed to the eighth abdominal segment.

(Degelmann et al., 1990)

vas¹ mutant embryos can develop into larvae and adults with a normal abdominal pattern, provided that they lack functional maternal transcripts of hb.

(Struhl, 1989)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

vas¹ is rescued by vas^Δ15-75.EGFP

(Dehghani and Lasko, 2016)

vas¹ is rescued by vas^Δ15-75.EGFP

(Dehghani and Lasko, 2016)

vas¹/vas³ is rescued by Scer\GAL4^VP16.nos.UTR/vas^UAS.GFP

(Xiol et al., 2014)

vas^PH165/vas¹ is rescued by vas^cKa.GFP

(Kugler et al., 2010)

vas^PH165/vas¹ is rescued by vas^5xAla.GFP

(Kugler et al., 2010)

vas¹/vas³ is rescued by vas^αTub67C.GFP

(Breitwieser et al., 1996)

Partially rescued by

vas¹ is partially rescued by vas^W660E.EGFP

(Dehghani and Lasko, 2016)

vas¹ is partially rescued by vas^W660E.EGFP

(Dehghani and Lasko, 2016)

vas¹ is partially rescued by vas^W660F.EGFP

(Dehghani and Lasko, 2016)

vas¹ is partially rescued by vas^W660F.EGFP

(Dehghani and Lasko, 2016)

Not rescued by

vas¹/vas³ is not rescued by Scer\GAL4^VP16.nos.UTR/vas^DQAD.UAS.GFP

(Xiol et al., 2014)

Comments

The loss of pole cells and patterning defects (assessed by no. of ftz stripes) characteristic for embryos from vas¹ homozygous mothers is rescued in progeny from vas¹;vas^{Δ15-75.T:Avic\GFP-EGFP} females, almost fully rescued in the progeny from vas¹;vas^{Δ15-75.T:Avic\GFP-EGFP} females and partially rescued in embryos from vas¹;vas^{Δ15-75.T:Avic\GFP-EGFP} mothers: the loss of pole cells is not restored but the patterning defects are ameliorated.

(Dehghani and Lasko, 2016)

Expression of vas^{DQAD.Scer\UAS.T:Avic\GFP} using Scer\GAL4^{nos.UTR.T:Hsim\VP16} fails to rescue the infertility phenotype of vas¹/vas³ mutants.

When vas^{Scer\UAS.T:Avic\GFP} is expressed using Scer\GAL4^{nos.UTR.T:Hsim\VP16} in a vas¹/vas³ mutant background, it rescues infertility. The rescued embryos show normal segmentation patterns, very similar to those hatched from eggs laid by wild-type females.

(Xiol et al., 2014)

Maternal expression of vas^{5xAla.T:Avic\GFP} rescues the viability and primordial germ cell formation defect in vas¹/vas^PH165 embryos.

Maternal expression of vas^{cKa.T:Avic\GFP} rescues the viability and primordial germ cell formation defect in vas¹/vas^PH165 embryos.

(Kugler et al., 2010)

vas^{αTub67C.T:Avic\GFP} rescues the abdominal and germ-line defects of vas¹/vas³ mutant embryos.

(Breitwieser et al., 1996)

Images (0)

Mutant

Wild-type

Stocks (7)

Bloomington

284

vas¹ cn¹ bw¹/CyO

2813

vas¹ cn¹ exu¹ bw¹/CyO

2620

vas¹ tor¹ cn¹ exu¹ bw¹/CyO

1574

vas¹ tor¹ cn¹ bw¹ speck¹/CyO

1735

vas¹ cn¹ tud¹ bw¹ speck¹/CyO

Kyoto

105832

vas¹ cn¹ bw¹/CyO

106477

vas¹ cn¹ tud¹ bw¹ speck¹/CyO

Notes on Origin

Discoverer

Comments

Germline mosaic analysis shows that vas is required in the germline.

(Schupbach and Wieschaus, 1986)

Cytoplasmic transplantation of wild type plasm into the abdominal region restores normal abdominal development.

(Lehmann and Nusslein-Volhard, 1991)

Although tud protein is present in mutant embryo extracts, its localization in the embryo is altered.

(Bardsley et al., 1993)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (6)

Reported As

Symbol Synonym

vas¹

(Dold et al., 2020, Dehghani and Lasko, 2016, Dehghani and Lasko, 2015, Liu et al., 2009, Moore et al., 2009, Christensen and Cook, 2007.3.22, Christensen and Cook, 2007.5.8)

vas^PD23

(Harris and Macdonald, 2001, Kashikawa et al., 1999, Schupbach and Wieschaus, 1991, Hay et al., 1990, Jack and McGinnis, 1990)

vas^PD

(Durdevic and Ephrussi, 2019, Little et al., 2015, Xiol et al., 2014, Morais-de-Sá et al., 2013, Kugler et al., 2010, Munro et al., 2006, Cinnamon et al., 2004, Forrest et al., 2004, Johnstone and Lasko, 2004, Forrest and Gavis, 2003, Bergsten et al., 2001, Clark et al., 2000, Crucs et al., 2000, Shulman et al., 2000, Bergsten and Gavis, 1999, Tomancak et al., 1998, Chen et al., 1997, Markussen et al., 1997, Dahanukar and Wharton, 1996, Gavis et al., 1996, Nakamura et al., 1996, Markussen et al., 1995, Rongo et al., 1995, Wolfgang and Forte, 1995, Clark et al., 1994, Ding et al., 1994, Gavis and Lehmann, 1994, Jongens et al., 1994, Liang et al., 1994, Bardsley et al., 1993, Jongens et al., 1992, Struhl et al., 1992, Ephrussi et al., 1991, Kim-Ha et al., 1991, Lehmann and Nusslein-Volhard, 1991, Riddihough and Ish-Horowicz, 1991, St. Johnston et al., 1991, Wharton and Struhl, 1991, Degelmann et al., 1990, Lasko and Ashburner, 1990, Lehmann, 1988, Tearle and Nusslein-Volhard, 1987, Schupbach and Wieschaus, 1986, Schupbach and Wieschaus, 1986)

vasPD

(Breitwieser et al., 1996)

vasa^PD23

(Kennerdell et al., 2002, Rittenhouse and Berg, 1995, Parkhurst and Ish-Horowicz, 1991, Raff et al., 1990, Tautz, 1988)

vasa^PD

(Johnstone et al., 2005, Newmark et al., 1997, Wilson et al., 1996, St. Johnston, 1993, Wang and Lehmann, 1991, Struhl, 1989, Driever and Nusslein-Volhard, 1988)

Name Synonyms

Secondary FlyBase IDs

References (73)

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