FB2025_01 , released February 20, 2025
Allele: Dmel\rho7M43
Open Close
General Information
Symbol
Dmel\rho7M43
Species
D. melanogaster
Name
FlyBase ID
FBal0017858
Feature type
allele
Associated gene
Dmel\rho
Associated Insertion(s)
Carried in Construct
Also Known As
rho7M, ve4, rhomboid7M43, rho4
Key Links
Allele class

amorphic allele - genetic evidence

Mutagen
Nature of the Allele
Allele class

amorphic allele - genetic evidence

(Kerber et al., 1998, Panzer et al., 1992, Klambt et al., 1991)
Mutagen
ethyl methanesulfonate
(Tearle and Nusslein-Volhard, 1987)
Progenitor genotype
Cytology
Description
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In

      abdominal lateral pentascolopidial chordotonal organ lch5

      (Elstob et al., 2001)

      anal pad

      (Tearle and Nusslein-Volhard, 1987)

      cephalopharyngeal skeleton

      (Tearle and Nusslein-Volhard, 1987)

      dorsal appendage | maternal effect | somatic clone

      (Zartman et al., 2009)

      embryonic/larval oenocyte

      (Elstob et al., 2001)

      embryonic anterior Malpighian tubule

      (Kerber et al., 1998)

      embryonic cardioblast (with rhoL68)

      (Schwarz et al., 2018)

      embryonic central brain

      (Page, 2003)

      embryonic Malpighian tubule

      (Harbecke and Lengyel, 1995)

      embryonic pericardial cell (with rhoL68)

      (Schwarz et al., 2018)

      embryonic posterior Malpighian tubule

      (Kerber et al., 1998)

      embryonic primordium of adult salivary gland

      (Panzer et al., 1992)

      embryonic ventral epidermis

      (Kim and Crews, 1993)

      embryonic ventral nerve cord

      (Tearle and Nusslein-Volhard, 1987)

      genital disc primordium

      (Chen et al., 2005)

      heart primordium (with rhoL68)

      (Schwarz et al., 2018)

      Keilin organ

      (Simcox, 1997, Tearle and Nusslein-Volhard, 1987)

      larval anterior commissure

      (Klambt et al., 1991)

      larval posterior commissure

      (Klambt et al., 1991)

      larval sense organ & antennal segment

      (Tearle and Nusslein-Volhard, 1987)

      larval sense organ & maxillary segment

      (Tearle and Nusslein-Volhard, 1987)

      larval thoracic perisympathetic organ

      (Zhou et al., 1997)

      lateral cervical tract

      (Page, 2003)

      mesoderm

      (Zhou et al., 1997)

      midline glial cell

      (Klambt et al., 1991)

      muscle cell of abdominal 2 dorsal acute muscle 1

      (Buff et al., 1998)

      muscle cell of abdominal 3 dorsal acute muscle 1

      (Buff et al., 1998)

      muscle cell of abdominal 4 dorsal acute muscle 1

      (Buff et al., 1998)

      muscle cell of abdominal 5 dorsal acute muscle 1

      (Buff et al., 1998)

      muscle cell of abdominal 6 dorsal acute muscle 1

      (Buff et al., 1998)

      muscle cell of abdominal 7 dorsal acute muscle 1

      (Buff et al., 1998)

      neuroblast

      (Skeath, 1998)

      photoreceptor cell R8

      (Birkholz et al., 2009)

      presumptive embryonic/larval tracheal system

      (Brodu and Casanova, 2006)

      symmetrical commissure

      (Xiao et al., 1996)

      tracheal primordium

      (Brodu and Casanova, 2006)

      ventral denticle belt

      (Xiao et al., 1996, Tearle and Nusslein-Volhard, 1987)

      ventral denticle belt | ventral

      (Simcox, 1997)
      Detailed Description
      Statement
      Reference

      rhoL68/rho7M43 mutant embryos exhibit cardioblast (CB) patterning defects, and a significantly decreased number of cardioblasts, namely of generic cardioblasts, leading to a lower generic CBs:ostial CBs ratio than the typical 4:2 ratio. These embryos also exhibit a significant decrease in the number of odd pericardial cells, although even pericardial cells are unaffected, as compared to controls.

      (Schwarz et al., 2018)

      R8 differentiation is defective in rho7M43 homozygotes.

      (Birkholz et al., 2009)

      Virtually all eggs derived from egg chambers containing complete homozygous follicle cell clones appear normal and have two dorsal appendages.

      (Lachance et al., 2009)

      Only 4% of eggs derived from females with mosaic rho7M43 egg chambers show defects in dorsal appendage size or inter-appendage distance.

      (Zartman et al., 2009)

      Tracheal invagination is alterated from its beginning at early stage 11 in rho7M43 mutant embryos. Instead of a few cells initiating invagination at a precise point and being subsequently followed by the remaining cells, a broad domain of the tracheal primordium appears to bend in a general way, forming a broad cavity. Cells on the dorsal side do not carry out their rotation movement, and an abnormal finger-like structure observed.

      In rho7M43 mutant embryos, neither myosin nor actin accumulates tightly around the invagination edge; instead they form aggregates.

      (Brodu and Casanova, 2006)

      In rho7M43 stage 15 embryos, the genital disc precursor cells are completely missing.

      (Chen et al., 2005)

      Metameric furrow form and are maintained normally in rho7M43 homozygous embryos.

      (Larsen et al., 2003)

      Mutants exhibit a loss of midbrain. Circumesophageal connectives are frequently broken at Embryonic Stage 16.

      (Page, 2003)

      Border cells still migrate dorsally when all dorsal follicle cells are mutant for rho7M43 in females containing homozygous clones.

      (Duchek and Rorth, 2001)

      The number of chordotonal organs in the lateral cluster is reduced from 5 to 3 in stage 16 homozygous embryos and there is a complete lack of oenocytes.

      (Elstob et al., 2001)

      Homozygous embryos lack muscle DA1 but not pericardial cells.

      (Buff et al., 1998)

      Homozygous embryos have a reduced number of cells in both the anterior and posterior Malpighian tubules compared to wild-type embryos. The number of cells in the anterior Malpighian tubules is severely reduced in rho7M43 SIIN double mutant embryos.

      (Kerber et al., 1998)

      rho6/rho7M43 embryos exhibit deletion of the ventral denticles so the width of the belt is reduced. Keilin's organs are missing or defective. Wing, leg, haltere and eye/antenna rho6/rho7M43 mutant discs can be in vivo cultured.

      (Simcox, 1997)

      Stage 12 embryos display lack of dorsal median cells in 7 out of 10 segments. Stage 14 embryos exhibit loss of mesodermal cells.

      (Zhou et al., 1997)

      Embryos lack denticles and often exhibit fusion between the medial regions of alternating denticle belts. CNS commissures are fused. Scer\GAL4sim.P3.7 mediated rhoScer\UAS.cXa expression in mutant embryos greatly reduces the medial fusion of denticle belts. The CNS phenotype is not rescued.

      (Xiao et al., 1996)

      Malpighian tubule elongation is incomplete in homozygous embryos.

      (Harbecke and Lengyel, 1995)

      Nondefective in gonad assembly.

      (Warrior, 1994)

      Salivary placodes are expanded towards the ventral midline.

      (Panzer et al., 1992)

      Anterior and posterior commissure fuse. Initial development of the axon commissures appears normal but the axon tracts fail to separate at the time of midline glia migration. Midline glial cells die early.

      (Klambt et al., 1991)

      No changes in phenotype of tor13D embryos.

      (Strecker et al., 1991)

      rho7M43 embryos have a narrow ventral nervous system and denticle bands. The medial parts of the denticle bands are fused. The ventral arms of the head skeleton are fused. The anal pads, Keilin's organs, maxillary sense organs and antennal sense organs are reduced.

      (Tearle and Nusslein-Volhard, 1987)

      strong allele embryonic lethal

      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Enhanced by
      Statement
      Reference

      rho7M43 has visible phenotype, enhanceable by ru1

      (Wasserman et al., 2000)
      Enhancer of
      Statement
      Reference

      rho7M43 is an enhancer of visible phenotype of ru1

      (Wasserman et al., 2000)
      NOT Enhancer of
      Statement
      Reference

      rho7M43 is a non-enhancer of lethal | embryonic stage | maternal effect phenotype of Gugunspecified

      (Zhang et al., 2002)
      Suppressor of
      Statement
      Reference

      rho7M43/rho[+] is a suppressor of visible phenotype of Scer\GAL4Tub.PU, cswN308D.UASp

      (Oishi et al., 2006)

      vn[+], vn1, rho7M43, rho[+] is a suppressor | partially of visible phenotype of Scer\GAL4en-e16E, tumΔE1E.UAS

      (Sotillos and Campuzano, 2000)
      Other
      Phenotype Manifest In
      Enhanced by
      Enhancer of
      Suppressor of
      Statement
      Reference

      rho7M43/rho[+] is a suppressor of eye phenotype of Scer\GAL4GMR.PS, cazKK107486

      (Shimamura et al., 2014)

      rho7M43/rho[+] is a suppressor of ommatidium phenotype of Scer\GAL4GMR.PS, cazKK107486

      (Shimamura et al., 2014)

      rho7M43/rho[+] is a suppressor of interommatidial bristle phenotype of Scer\GAL4GMR.PS, cazKK107486

      (Shimamura et al., 2014)

      rho7M43/rho[+] is a suppressor of cone cell phenotype of Scer\GAL4GMR.PS, cazKK107486

      (Shimamura et al., 2014)

      rho7M43/rho[+] is a suppressor of wing vein | ectopic phenotype of Scer\GAL4Tub.PU, cswN308D.UASp

      (Oishi et al., 2006)

      vn[+], vn1, rho7M43, rho[+] is a suppressor | partially of wing phenotype of Scer\GAL4en-e16E, tumΔE1E.UAS

      (Sotillos and Campuzano, 2000)

      rho7M43/rho[+] is a suppressor of phenotype of Src42ASu(Raf)1-1

      (Zhang et al., 1999)
      NOT Suppressor of
      Statement
      Reference

      rho7M43/ru1 is a non-suppressor of photoreceptor cell R1 phenotype of raspT802

      (Steinhauer et al., 2013)

      rho7M43/ru1 is a non-suppressor of photoreceptor cell R2 phenotype of raspT802

      (Steinhauer et al., 2013)

      rho7M43/ru1 is a non-suppressor of photoreceptor cell R3 phenotype of raspT802

      (Steinhauer et al., 2013)

      rho7M43/ru1 is a non-suppressor of photoreceptor cell R4 phenotype of raspT802

      (Steinhauer et al., 2013)

      rho7M43/ru1 is a non-suppressor of photoreceptor cell R5 phenotype of raspT802

      (Steinhauer et al., 2013)

      rho7M43/ru1 is a non-suppressor of photoreceptor cell R6 phenotype of raspT802

      (Steinhauer et al., 2013)

      rho7M43/ru1 is a non-suppressor of photoreceptor cell R7 phenotype of raspT802

      (Steinhauer et al., 2013)
      Other
      Statement
      Reference

      btlLG19, rho7M43 has presumptive embryonic/larval tracheal system phenotype

      (Brodu and Casanova, 2006)

      btlLG19, rho7M43 has tracheal primordium phenotype

      (Brodu and Casanova, 2006)

      rho7M43, ru1 has ommatidial precursor cluster | somatic clone phenotype

      (Brown et al., 2006)

      Gug14967, aosΔ7, rho7M43, ru1 has eye photoreceptor cell | somatic clone phenotype

      (Charroux et al., 2006)

      rho7M43, ru1 has photoreceptor cell R7 | somatic clone phenotype

      (Frankfort and Mardon, 2004)

      rho7M43, ru1 has photoreceptor cell R1 | somatic clone phenotype

      (Frankfort and Mardon, 2004)

      rho7M43, ru1 has photoreceptor cell R6 | somatic clone phenotype

      (Frankfort and Mardon, 2004)

      rho7M43, ru1 has photoreceptor cell R3 | somatic clone phenotype

      (Frankfort and Mardon, 2004, Yang and Baker, 2003)

      rho7M43, ru1 has photoreceptor cell R2 | somatic clone phenotype

      (Frankfort and Mardon, 2004, Yang and Baker, 2003)

      rho7M43, ru1 has photoreceptor cell R4 | somatic clone phenotype

      (Frankfort and Mardon, 2004, Yang and Baker, 2003)

      rho7M43, ru1 has photoreceptor cell R5 | somatic clone phenotype

      (Frankfort and Mardon, 2004, Yang and Baker, 2003)

      rho7M43, ru1, vnL6 has tarsal segment phenotype

      (Campbell, 2002)

      rho7M43, vnddd-4 has neuroblast phenotype

      (Skeath, 1998)

      rho7M43, vnddd-4 has larval RP2 motor neuron phenotype

      (Skeath, 1998)

      rho7M43, vnddd-4 has RP2sib neuron phenotype

      (Skeath, 1998)

      rho7M43, vnddd-4 has neuroblast MP2 phenotype

      (Skeath, 1998)

      rho7M43, vnddd-4 has neuroblast NB7-1 phenotype

      (Skeath, 1998)

      rho7M43, vnddd-4 has neuroblast NB3-2 phenotype

      (Skeath, 1998)

      rho7M43, vnddd-4 has neuroblast NB5-2 phenotype

      (Skeath, 1998)

      rho7M43, vnddd-4 has neuroblast NB1-1 phenotype

      (Skeath, 1998)
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      A rho7M43 heterozygous background significantly suppresses the cazKK107486-induced rough eye phenotype and rescues the fusion of cone cells in pupal retinae.

      (Shimamura et al., 2014)

      A ru1 rho7M43 double mutant background fails to suppress the R1-R7 photoreceptor differentiation defects seen in raspT802 mutant eye disc clones.

      (Steinhauer et al., 2013)

      ru1/+, rho7M43/+ mutants have normal cardiac function.

      (Yu et al., 2010)

      Tracheal invagination initiates in rho7M43 btlLG19 double mutants, but rotation of dorsal cells does not occur, as in rho7M43 single mutants. However, a finger-like structure is never formed, instead, the tracheal cells remain as a cavity.

      (Brodu and Casanova, 2006)

      ru1 rho7M43 Minute clones in the eye disc fail to form the arcs and rosettes of cells in the morphogenetic furrow that are seen in wild-type eye discs. Small clusters of cells can very occasionally be observed in the furrow but these appear at much lower levels than in wild type.

      (Brown et al., 2006)

      The extra photoreceptor cell phenotype of argosΔ7 clones is enhanced in argosΔ7 Gug14967 clones, with a greater percentage of ommatidia showing supernumerary photoreceptor cells. In contrast, ru1 rho7M43 Gug14967 argosΔ7 mutant clones lack photoreceptor cells.

      (Charroux et al., 2006)

      ru1, rho7M43 embryos show an increased level of apoptosis.

      (Parker, 2006)

      In rho7M43; ru1 double mutant clones in the eye disc, only R8 photoreceptor cells differentiate In rho7M43; ru1; sensE2 triple mutant clones, no photoreceptors differentiate except for a few photoreceptors near the clonal boundary, presumably rescued non-autonomously by neighboring wild-type cell. No rescue of photoreceptor development is seen when these triple mutant clones are made in a rox63 homozygous background.

      (Frankfort and Mardon, 2004)

      69% of cuticles from rho7M43; ru1 double homozygous embryos have at least one denticle belt fusion, compared with 30% in rhounspecified homozygotes. Other aspects of the cuticle phenotype in these double mutants are no stronger than those in rhounspecified embryos. (Note, while the authors do not name an rho allele for this analysis, they do claim to have used a null allele.) Denticle belt fusions in the cuticles of rho7M43; ru1 double homozygous embryos are suppressed by Egfr::tort4021E.hs.sev or Ras85DV12.Scer\UAS with Scer\GAL4prd.RG1, and enhanced by EgfrDN.Scer\UAS (P{UAS-Egfr.DN}29-77-1), Ras85DN17.Scer\UAS or phlK497M.Scer\UAS with Scer\GAL4prd.RG1. The penetrance of denticle belt fusions in the cuticles of rho7M43; ru1 double homozygous embryos is reduced from just under 60% to less than 20% by Df(3L)H99/Df(3L)H99.

      (Urban et al., 2004)

      In rho7M43; ru1 double mutant somatic clones in the 3rd instar eye disc, photoreceptors R2-R5 fail to undergo G1 arrest or differentiation.

      (Yang and Baker, 2003)

      Large ru1 rho7M43 vnL6 triple mutant clones in the leg can result in truncations of the tarsus region (for example only three tarsal segments may be present). Mosaic legs which have wild-type tissue at the distal tip show rescue of tarsal development.

      (Campbell, 2002)

      Weakly enhances the eye phenotype produced by activated arm constructs. (either armS44Y.GMR or armS56F.GMR).

      (Freeman and Bienz, 2001)

      Clones of doubly mutant for rho7M43 and ru1 do not survive into adult eyes. When mosaic ommatidia are studied, no mutant R8 photoreceptor cells are seen, while mutant photoreceptor cells R1-7 are seen in between a third and a half of mosaic ommatidia. When imaginal discs are examined, an absence of non-R8 photoreceptor cells are seen, and also a complete loss of cone cells. In these clones an increased level of apoptosis is also seen. Apoptotic cells are seen in two main zones, one just ahead of the advancing morphogenetic furrow, and one towards the posterior of the clone.

      (Wasserman et al., 2000)

      Dominantly suppresses the ability of Src42ASu(phl)1-1 to suppress the lethality of phl1/Y flies.

      (Zhang et al., 1999)

      rho7M43, vnddd-4 double mutant embryos show embryonic CNS neuroblast phenotypes that are indistinguishable from those cause by Egfr mutants. Only two neuroblast columns form, RP2 motorneuron almost never forms, half of the MP2s are missing. The 5-2 and MP2 phenotypes are slightly stronger than for Egfrf2.

      (Skeath, 1998)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Fails to complement

      rho6

      (Zartman et al., 2009)

      rho6

      (Bier et al., 1990)

      rho5

      (Bier et al., 1990)
      Partially rescued by

      rho7M43 is partially rescued by Scer\GAL4rho.654/rhoUAS.cGa

      (Li-Kroeger et al., 2008)

      rho7M43 is partially rescued by rhoUAS.cXa/Scer\GAL4sim.P3.7

      (Xiao et al., 1996)

      rho7M43 is partially rescued by rhoRyn

      (Bier et al., 1990)
      Comments

      rhoScer\UAS.cGa driven by Scer\GAL4rho.654 rescues the oenocyte defect but not the secondary sensory organ precursor cell defects associated with rho7M43.

      (Li-Kroeger et al., 2008)

      rho7M43 is both partially rescued by two copies of rhoRyn : 40% of such embryos hatch as viable larvae, with rescued cuticular phenotype. Rescued larvae do not survive to adulthood.

      (Bier et al., 1990)
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer

      Jurgens.

      Associated with: ru1.

      (Page, 2003)
      Comments
      Comments

      Less than 10% of wild type number of ventral epidermal cells expressing P{lacZ}BP28 are evident in mutant embryos. oc expression is greatly reduced along the midline.

      (Kim and Crews, 1993)
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (16)
      References (67)