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Allele Dmel\rho^7M43

General Information
Symbol	Dmel\rho7M43	Species	D. melanogaster
Name		FlyBase ID	FBal0017858
Feature type	allele	Associated gene	Dmel\rho
Also Known As	rho7M, ve4
Allele class	amorphic allele - genetic evidence
Mutagen	ethyl methanesulfonate
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class	amorphic allele - genetic evidence (Klambt et al., 1991, Panzer et al., 1992, Kerber et al., 1998)
Mutagen	ethyl methanesulfonate (Tearle and Nusslein-Volhard, 1987)
Mutations Mapped to the Genome
Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL
Progenitor genotype
Nature of the lesion	Statement Reference
Cytology
Phenotypic Data
Phenotypic Class
	lethal | embryonic stage | recessive (Bier et al., 1990, Tearle and Nusslein-Volhard, 1987)
Phenotype Manifest In
	abdominal 2 dorsal acute muscle 1 (Buff et al., 1998) abdominal 3 dorsal acute muscle 1 (Buff et al., 1998) abdominal 4 dorsal acute muscle 1 (Buff et al., 1998) abdominal 5 dorsal acute muscle 1 (Buff et al., 1998) abdominal 6 dorsal acute muscle 1 (Buff et al., 1998) abdominal 7 dorsal acute muscle 1 (Buff et al., 1998) abdominal lateral pentascolopidial chordotonal organ lch5 (Elstob et al., 2001) adult salivary gland primordium (Panzer et al., 1992) anal pad (Tearle and Nusslein-Volhard, 1987) anterior commissure (Klambt et al., 1991) cephalopharyngeal skeleton (Tearle and Nusslein-Volhard, 1987) circumesophageal tract | precursor (Page, 2003) commissure (Xiao et al., 1996) denticle belt (Tearle and Nusslein-Volhard, 1987, Xiao et al., 1996) denticle belt | ventral (Simcox, 1997) dorsal appendage | maternal effect | somatic clone (Zartman et al., 2009) dorsal neurohemal organ (Zhou et al., 1997) embryonic/larval oenocyte (Elstob et al., 2001) embryonic anterior Malpighian tubule (Kerber et al., 1998) embryonic central brain (Page, 2003) embryonic Malpighian tubule (Harbecke and Lengyel, 1995) embryonic posterior Malpighian tubule (Kerber et al., 1998) embryonic ventral epidermis (Kim and Crews, 1993) embryonic ventral nervous system (Tearle and Nusslein-Volhard, 1987) genital disc primordium (Chen et al., 2005) Keilin's organ (Tearle and Nusslein-Volhard, 1987, Simcox, 1997) larval sense organ & antennal segment (Tearle and Nusslein-Volhard, 1987) larval sense organ & maxillary segment (Tearle and Nusslein-Volhard, 1987) mesoderm (Zhou et al., 1997) midline glial cell (Klambt et al., 1991) neuroblast (Skeath, 1998) photoreceptor cell R8 (Birkholz et al., 2009) posterior commissure (Klambt et al., 1991) presumptive embryonic/larval tracheal system (Brodu and Casanova, 2006) tracheal primordium (Brodu and Casanova, 2006)
Detailed Description
	Statement Reference R8 differentiation is defective in rho[7M43] homozygotes. (Birkholz et al., 2009) Virtually all eggs derived from egg chambers containing complete homozygous follicle cell clones appear normal and have two dorsal appendages. (Lachance et al., 2009) Only 4% of eggs derived from females with mosaic rho[7M43] egg chambers show defects in dorsal appendage size or inter-appendage distance. (Zartman et al., 2009) Tracheal invagination is alterated from its beginning at early stage 11 in rho7M43 mutant embryos. Instead of a few cells initiating invagination at a precise point and being subsequently followed by the remaining cells, a broad domain of the tracheal primordium appears to bend in a general way, forming a broad cavity. Cells on the dorsal side do not carry out their rotation movement, and an abnormal finger-like structure observed. In rho7M43 mutant embryos, neither myosin nor actin accumulates tightly around the invagination edge; instead they form aggregates. (Brodu and Casanova, 2006) In rho7M43 stage 15 embryos, the genital disc precursor cells are completely missing. (Chen et al., 2005) Metameric furrow form and are maintained normally in rho7M43 homozygous embryos. (Larsen et al., 2003) Mutants exhibit a loss of midbrain. Circumesophageal connectives are frequently broken at Embryonic Stage 16. (Page, 2003) Border cells still migrate dorsally when all dorsal follicle cells are mutant for rho7M43 in females containing homozygous clones. (Duchek and Rorth, 2001) The number of chordotonal organs in the lateral cluster is reduced from 5 to 3 in stage 16 homozygous embryos and there is a complete lack of oenocytes. (Elstob et al., 2001) Homozygous embryos lack muscle DA1 but not pericardial cells. (Buff et al., 1998) Homozygous embryos have a reduced number of cells in both the anterior and posterior Malpighian tubules compared to wild-type embryos. The number of cells in the anterior Malpighian tubules is severely reduced in rho7M43 SIIN double mutant embryos. (Kerber et al., 1998) rho6/rho7M43 embryos exhibit deletion of the ventral denticles so the width of the belt is reduced. Keilin's organs are missing or defective. Wing, leg, haltere and eye/antenna rho6/rho7M43 mutant discs can be in vivo cultured. (Simcox, 1997) Stage 12 embryos display lack of dorsal median cells in 7 out of 10 segments. Stage 14 embryos exhibit loss of mesodermal cells. (Zhou et al., 1997) Embryos lack denticles and often exhibit fusion between the medial regions of alternating denticle belts. CNS commissures are fused. Scer\GAL4sim.P3.7 mediated rhoScer\UAS.cXa expression in mutant embryos greatly reduces the medial fusion of denticle belts. The CNS phenotype is not rescued. (Xiao et al., 1996) Malpighian tubule elongation is incomplete in homozygous embryos. (Harbecke and Lengyel, 1995) Nondefective in gonad assembly. (Warrior, 1994) Salivary placodes are expanded towards the ventral midline. (Panzer et al., 1992) Anterior and posterior commissure fuse. Initial development of the axon commissures appears normal but the axon tracts fail to separate at the time of midline glia migration. Midline glial cells die early. (Klambt et al., 1991) No changes in phenotype of tor13D embryos. (Strecker et al., 1991) rho7M43 embryos have a narrow ventral nervous system and denticle bands. The medial parts of the denticle bands are fused. The ventral arms of the head skeleton are fused. The anal pads, Keilin's organs, maxillary sense organs and antennal sense organs are reduced. (Tearle and Nusslein-Volhard, 1987) strong allele embryonic lethal
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement Reference rho7M43 has visible phenotype, enhanceable by ru1 (Wasserman et al., 2000)
Enhancer of
Statement Reference rho7M43 is an enhancer of visible phenotype of ru1 (Wasserman et al., 2000)
NOT Enhancer of
Statement Reference rho7M43 is a non-enhancer of lethal | maternal effect | embryonic stage phenotype of Gugunspecified (Zhang et al., 2002)
Suppressor of
Statement Reference rho7M43/rho+ is a suppressor of visible phenotype of Scer\GAL4tub.PU, cswN308D.Scer\UAS.P\T (Oishi et al., 2006) vn[+], vn1, rho7M43, rho[+] is a suppressor | partially of visible phenotype of tumΔE1E.Scer\UAS, Scer\GAL4en-e16E (Sotillos and Campuzano, 2000)
Other
Statement Reference rho7M43, ru1, vnL6 has visible phenotype (Campbell, 2002, Campbell, 2002, Campbell, 2002) rho7M43, ru1 has cell death defective | somatic clone phenotype (Wasserman et al., 2000, Wasserman et al., 2000) rho7M43, ru1 has cell lethal | somatic clone phenotype (Wasserman et al., 2000, Wasserman et al., 2000) rho7M43, ru1 has increased cell death | embryonic stage phenotype (Parker, 2006) rho7M43, ru1 has mitotic cell cycle defective | somatic clone | cell non-autonomous phenotype (Yang and Baker, 2003, Yang and Baker, 2003) rho7M43, ru1 has mitotic cell cycle defective | somatic clone phenotype (Yang and Baker, 2003, Yang and Baker, 2003)
Phenotype Manifest In
Enhanced by
Statement Reference rho7M43, ru1 has photoreceptor cell | somatic clone phenotype, enhanceable by sensE2 (Frankfort and Mardon, 2004) rho7M43 has cone cell | somatic clone phenotype, enhanceable by ru1 (Wasserman et al., 2000) rho7M43 has eye | somatic clone phenotype, enhanceable by ru1 (Wasserman et al., 2000) rho7M43 has photoreceptor cell | somatic clone phenotype, enhanceable by ru1 (Wasserman et al., 2000) rho7M43 has photoreceptor cell R1 | somatic clone phenotype, enhanceable by ru1 (Wasserman et al., 2000) rho7M43 has photoreceptor cell R2 | somatic clone phenotype, enhanceable by ru1 (Wasserman et al., 2000) rho7M43 has photoreceptor cell R3 | somatic clone phenotype, enhanceable by ru1 (Wasserman et al., 2000) rho7M43 has photoreceptor cell R4 | somatic clone phenotype, enhanceable by ru1 (Wasserman et al., 2000) rho7M43 has photoreceptor cell R5 | somatic clone phenotype, enhanceable by ru1 (Wasserman et al., 2000) rho7M43 has photoreceptor cell R6 | somatic clone phenotype, enhanceable by ru1 (Wasserman et al., 2000) rho7M43 has photoreceptor cell R7 | somatic clone phenotype, enhanceable by ru1 (Wasserman et al., 2000) rho7M43 has photoreceptor cell R8 | somatic clone | cell non-autonomous phenotype, enhanceable by ru1 (Wasserman et al., 2000)
Enhancer of
Statement Reference rho7M43/rho7M43 is an enhancer of eye phenotype of ru1 (Birkholz et al., 2009, Birkholz et al., 2009) rho7M43/rho7M43 is an enhancer of ommatidium phenotype of ru1 (Birkholz et al., 2009, Birkholz et al., 2009) rho7M43 is an enhancer of cone cell | somatic clone phenotype of ru1 (Wasserman et al., 2000) rho7M43 is an enhancer of eye | somatic clone phenotype of ru1 (Wasserman et al., 2000) rho7M43 is an enhancer of photoreceptor cell | somatic clone phenotype of ru1 (Wasserman et al., 2000) rho7M43 is an enhancer of photoreceptor cell R1 | somatic clone phenotype of ru1 (Wasserman et al., 2000) rho7M43 is an enhancer of photoreceptor cell R2 | somatic clone phenotype of ru1 (Wasserman et al., 2000) rho7M43 is an enhancer of photoreceptor cell R3 | somatic clone phenotype of ru1 (Wasserman et al., 2000) rho7M43 is an enhancer of photoreceptor cell R4 | somatic clone phenotype of ru1 (Wasserman et al., 2000) rho7M43 is an enhancer of photoreceptor cell R5 | somatic clone phenotype of ru1 (Wasserman et al., 2000) rho7M43 is an enhancer of photoreceptor cell R6 | somatic clone phenotype of ru1 (Wasserman et al., 2000) rho7M43 is an enhancer of photoreceptor cell R7 | somatic clone phenotype of ru1 (Wasserman et al., 2000) rho7M43 is an enhancer of photoreceptor cell R8 | somatic clone | cell non-autonomous phenotype of ru1 (Wasserman et al., 2000)
Suppressor of
Statement Reference rho7M43/rho[+] is a suppressor of phenotype of Src42ASu(phl)1-1 (Zhang et al., 1999) rho7M43/rho+ is a suppressor of wing vein | ectopic phenotype of Scer\GAL4tub.PU, cswN308D.Scer\UAS.P\T (Oishi et al., 2006) vn[+], vn1, rho7M43, rho[+] is a suppressor | partially of wing phenotype of tumΔE1E.Scer\UAS, Scer\GAL4en-e16E (Sotillos and Campuzano, 2000)
Other
Statement Reference btlLG19, rho7M43 has presumptive embryonic/larval tracheal system phenotype (Brodu and Casanova, 2006) btlLG19, rho7M43 has tracheal primordium phenotype (Brodu and Casanova, 2006) Gug14967, aosΔ7, rho7M43, ru1 has eye photoreceptor cell | somatic clone phenotype (Charroux et al., 2006, Charroux et al., 2006, Charroux et al., 2006, Charroux et al., 2006) rho7M43, ru1, vnL6 has tarsal segment phenotype (Campbell, 2002, Campbell, 2002, Campbell, 2002) rho7M43, ru1 has ommatidial precursor cluster | somatic clone phenotype (Brown et al., 2006) rho7M43, ru1 has photoreceptor cell R1 | somatic clone phenotype (Frankfort and Mardon, 2004, Frankfort and Mardon, 2004) rho7M43, ru1 has photoreceptor cell R2 | somatic clone phenotype (Yang and Baker, 2003, Yang and Baker, 2003, Frankfort and Mardon, 2004, Frankfort and Mardon, 2004) rho7M43, ru1 has photoreceptor cell R3 | somatic clone phenotype (Yang and Baker, 2003, Yang and Baker, 2003, Frankfort and Mardon, 2004, Frankfort and Mardon, 2004) rho7M43, ru1 has photoreceptor cell R4 | somatic clone phenotype (Yang and Baker, 2003, Yang and Baker, 2003, Frankfort and Mardon, 2004, Frankfort and Mardon, 2004) rho7M43, ru1 has photoreceptor cell R5 | somatic clone phenotype (Yang and Baker, 2003, Yang and Baker, 2003, Frankfort and Mardon, 2004, Frankfort and Mardon, 2004) rho7M43, ru1 has photoreceptor cell R6 | somatic clone phenotype (Frankfort and Mardon, 2004, Frankfort and Mardon, 2004) rho7M43, ru1 has photoreceptor cell R7 | somatic clone phenotype (Frankfort and Mardon, 2004, Frankfort and Mardon, 2004) rho7M43, vnddd-4 has neuroblast MP2 phenotype (Skeath, 1998, Skeath, 1998) rho7M43, vnddd-4 has neuroblast NB1-1 phenotype (Skeath, 1998, Skeath, 1998) rho7M43, vnddd-4 has neuroblast NB3-2 phenotype (Skeath, 1998, Skeath, 1998) rho7M43, vnddd-4 has neuroblast NB5-2 phenotype (Skeath, 1998, Skeath, 1998) rho7M43, vnddd-4 has neuroblast NB7-1 phenotype (Skeath, 1998, Skeath, 1998) rho7M43, vnddd-4 has neuroblast phenotype (Skeath, 1998, Skeath, 1998) rho7M43, vnddd-4 has RP2 motor neuron phenotype (Skeath, 1998, Skeath, 1998) rho7M43, vnddd-4 has RP2sib neuron phenotype (Skeath, 1998, Skeath, 1998)
Additional Comments
Genetic Interactions
Statement Reference ru[1]/+, rho[7M43]/+ mutants have normal cardiac function. (Yu et al., 2010) Tracheal invagination initiates in rho7M43 btlLG19 double mutants, but rotation of dorsal cells does not occur, as in rho7M43 single mutants. However, a finger-like structure is never formed, instead, the tracheal cells remain as a cavity. (Brodu and Casanova, 2006) ru1 rho7M43 Minute clones in the eye disc fail to form the arcs and rosettes of cells in the morphogenetic furrow that are seen in wild-type eye discs. Small clusters of cells can very occasionally be observed in the furrow but these appear at much lower levels than in wild type. (Brown et al., 2006) The extra photoreceptor cell phenotype of argosΔ7 clones is enhanced in argosΔ7 Gug14967 clones, with a greater percentage of ommatidia showing supernumerary photoreceptor cells. In contrast, ru1 rho7M43 Gug14967 argosΔ7 mutant clones lack photoreceptor cells. (Charroux et al., 2006) ru1, rho7M43 embryos show an increased level of apoptosis. (Parker, 2006) In rho7M43; ru1 double mutant clones in the eye disc, only R8 photoreceptor cells differentiate In rho7M43; ru1; sensE2 triple mutant clones, no photoreceptors differentiate except for a few photoreceptors near the clonal boundary, presumably rescued non-autonomously by neighboring wild-type cell. No rescue of photoreceptor development is seen when these triple mutant clones are made in a rox63 homozygous background. (Frankfort and Mardon, 2004) 69% of cuticles from rho7M43; ru1 double homozygous embryos have at least one denticle belt fusion, compared with 30% in rhounspecified homozygotes. Other aspects of the cuticle phenotype in these double mutants are no stronger than those in rhounspecified embryos. (Note, while the authors do not name an rho allele for this analysis, they do claim to have used a null allele.) Denticle belt fusions in the cuticles of rho7M43; ru1 double homozygous embryos are suppressed by Egfr::tort4021E.hs.sev or Ras85DV12.Scer\UAS with Scer\GAL4prd.RG1, and enhanced by EgfrDN.Scer\UAS (P{UAS-Egfr.DN}29-77-1), Ras85DN17.Scer\UAS or phlK497M.Scer\UAS with Scer\GAL4prd.RG1. The penetrance of denticle belt fusions in the cuticles of rho7M43; ru1 double homozygous embryos is reduced from just under 60% to less than 20% by Df(3L)H99/Df(3L)H99. (Urban et al., 2004) In rho7M43; ru1 double mutant somatic clones in the 3rd instar eye disc, photoreceptors R2-R5 fail to undergo G1 arrest or differentiation. (Yang and Baker, 2003) Large ru1 rho7M43 vnL6 triple mutant clones in the leg can result in truncations of the tarsus region (for example only three tarsal segments may be present). Mosaic legs which have wild-type tissue at the distal tip show rescue of tarsal development. (Campbell, 2002) Weakly enhances the eye phenotype produced by activated arm constructs. (either armS44Y.GMR or armS56F.GMR). (Freeman and Bienz, 2001) Clones of doubly mutant for rho7M43 and ru1 do not survive into adult eyes. When mosaic ommatidia are studied, no mutant R8 photoreceptor cells are seen, while mutant photoreceptor cells R1-7 are seen in between a third and a half of mosaic ommatidia. When imaginal discs are examined, an absence of non-R8 photoreceptor cells are seen, and also a complete loss of cone cells. In these clones an increased level of apoptosis is also seen. Apoptotic cells are seen in two main zones, one just ahead of the advancing morphogenetic furrow, and one towards the posterior of the clone. (Wasserman et al., 2000) Dominantly suppresses the ability of Src42ASu(phl)1-1 to suppress the lethality of phl1/Y flies. (Zhang et al., 1999) rho7M43, vnddd-4 double mutant embryos show embryonic CNS neuroblast phenotypes that are indistinguishable from those cause by Egfr mutants. Only two neuroblast columns form, RP2 motorneuron almost never forms, half of the MP2s are missing. The 5-2 and MP2 phenotypes are slightly stronger than for Egfrf2. (Skeath, 1998)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Fails to complement	rho5 (Bier et al., 1990) rho6 (Bier et al., 1990, Zartman et al., 2009)
Partially rescued by	rho7M43 is partially rescued by rhoRyn (Bier et al., 1990) rho7M43 is partially rescued by rhoScer\UAS.cXa/Scer\GAL4sim.P3.7 (Xiao et al., 1996) rho7M43 is partially rescued by Scer\GAL4rho.654/rhoScer\UAS.cGa (Li-Kroeger et al., 2008)
Comments	rho[Scer\UAS.cGa] driven by Scer\GAL4[rho.654] rescues the oenocyte defect but not the secondary sensory organ precursor cell defects associated with rho[7M43]. (Li-Kroeger et al., 2008) rho7M43 is both partially rescued by two copies of rhoRyn: 40% of such embryos hatch as viable larvae, with rescued cuticular phenotype. Rescued larvae do not survive to adulthood. (Bier et al., 1990)
Stocks ( 1 )
Bloomington	1471 ru1 rho7M43 h1 th1 st1 cu1 sr1 es ca1/TM3, Sb1
Notes on Origin
Discoverer	Jurgens.
	Associated with: ru1. (Page, 2003)
Comments
	Less than 10% of wild type number of ventral epidermal cells expressing P{lacZ}BP28 are evident in mutant embryos. oc expression is greatly reduced along the midline. (Kim and Crews, 1993)
External Crossreferences & Linkouts
Other Crossreferences
Linkouts
Synonyms & Secondary IDs ( 14 )
Reported As
Symbol Synonym	rho17M43 (Yang and Baker, 2003) rho-17M43 (Wasserman et al., 2000) rho7M3 (Brodu and Casanova, 2006) rho7M43 (Atkey et al., 2006, Charroux et al., 2006, Brown et al., 2006, Lachance et al., 2009, Li-Kroeger et al., 2008, Yu et al., 2010, Moses et al., 2011, Oishi et al., 2006) rho7m43 (Parker, 2006) rho7M (Fusse and Hoch, 2002, von Ohlen and Doe, 2000, Wessells et al., 1999, Spencer et al., 1998, Chen et al., 1998, Buff et al., 1998, Billin and Poole, 1995, Warrior, 1994, Tearle and Nusslein-Volhard, 1987, Zartman et al., 2009) rho7m (Zhang et al., 1999) rhove-4   rhomboid7M43 (Page, 2003) rhomboid7M (Skeath, 1998) ve4 (Harbecke and Lengyel, 1995) ve4 (Jack and Myette, 1999, Kerber et al., 1998, Kim and Crews, 1993, Mayer and Nusslein-Volhard, 1988, ) ve7M43   ve7M (Sotillos and Campuzano, 2000)
Name Synonym	rhomboid7M43 (Atkey et al., 2006)
Secondary FlyBase IDs
References ( 53 )
Generate a list of	All references relating to this allele ( 53 )
List References by type	Research paper  ( 52 ) Stock list  ( 1 )
Recent research papers ( 1 )
	Moses et al., 2011, Dev. Biol. 360(1): 208--215 Phosphorylation of Ind by MAP kinase enhances Ind-dependent transcriptional repression. [FBrf0216621] Show all research papers ...