Approximate boundaries of vg83b27, a deletion which removes most of intron 2 but does not alter the surrounding exons.
Polytene chromosomes normal.
Deletion of the vg "boundary enhancer".
Intronic deletion.
Deletion of intron 2.
Deletion of a major part of intron 2.
Deletion of DNA.
Deletes intronic regulatory element within intron 2.
Deletion of vg sequences. 5' end of the deletion maps at least 500bp from the 3' end of the upstream exon and the 3' end maps quite close to the 5' end of the downstream exon.
3kb deletion from + 5 to + 8; '+' values to right (distal); 0 coordinate assigned to insert position of vg21; Williams, Atkin and Bell (1990)
vg83b27 mutants do not develop a wing pouch.
Mature mutant wing discs lack the wing primordium.
Homozygous clones induced in mid- to late second instar larvae are able to contribute normally to the wing pouch. However, homozygous clones induced at earlier times show a progressive decrease in their ability to do so. Homozygous clones induced during the first larval instar and given a competitive growth advantage using the Minute technique appear to be excluded from the wing pouch, forming non-wing tissue at its expense.
Mutants show no growth of wing blade tissue, although some hinge structures are still present.
Homozygotes lack halteres and wings are almost always absent.
Homozygous flies have virtually no wings.
Significant cell death is not seen in the third larval instar wing disc (similar to wild type).
The formation of ectopic wing-like outgrowths caused by expression of vgScer\UAS.cZa under the control of Scer\GAL4ptc-559.1 is more strongly reduced in a vg83b27 homozygous background than in a vg83b27 heterozygous background.
Wing phenotype is unaffected by temperature.
Flies have knobbed wings and halteres.
Homozygotes display high rate of resistance to fluorodeoxyuridine (FUdR), an inhibitor of thymidylate synthetase and slight resistance to aminopterin.
Not suppressed by Su(vg)B271, Su(vg)E41 or Su(vg)E61.
Wing and haltere structures eliminated: cell death of larval imaginal discs.
Homozygotes show strong wing and haltere reductions. They are wild type with respect to the postscutellar bristle and female fertility phenotypes. Transheterozygotes with vg1 exhibit wing bubbles and unequal wing deficiencies. The complementation behaviour is unlikely to be mediated by a transvection-like process.
Homozygous viable, but with an extreme vg phenotype.
Homozygotes have a vgnw wing phenotype. Postscutellars are wild-type, and halteres are missing.
homozygous wing phenotype like that of vgnw halteres absent postscutellar bristles present homozygous viable female-fertile?
vg83b27/vg1 has visible phenotype, enhanceable by In(2LR)789
vg83b27/vg1 has visible phenotype, enhanceable by T(2;3)PIIA
vg83b27/vg1 has visible phenotype, enhanceable by T(2;3)PIIB
vg83b27/vg1 has visible phenotype, enhanceable by Tp(2;3)P10.C
vg83b27 has visible phenotype, suppressible | partially by E2f1UAS.cNa/DpUAS.cDa/Scer\GAL4vg.int2.1/BacA\p35UAS.cHa
vg83b27 has visible | recessive phenotype, suppressible | partially by Hsap\VGLL1UAS.cVa/Scer\GAL4vg.int2.1
vg83b27 has visible phenotype, non-suppressible by Scer\GAL4vg.PU/BacA\p35UAS.cHa
vg83b27/vg1 has haltere phenotype, enhanceable by In(2LR)789
vg83b27/vg1 has wing phenotype, enhanceable by In(2LR)789
vg83b27/vg1 has haltere phenotype, enhanceable by T(2;3)PIIA
vg83b27/vg1 has wing phenotype, enhanceable by T(2;3)PIIA
vg83b27/vg1 has haltere phenotype, enhanceable by T(2;3)PIIB
vg83b27/vg1 has haltere phenotype, enhanceable by Tp(2;3)P10.C
vg83b27/vg1 has wing phenotype, enhanceable by Tp(2;3)P10.C
vg83b27/vg1 has wing phenotype, non-enhanceable by T(2;3)PIIB
vg83b27 has wing blade phenotype, suppressible | partially by E2f1UAS.cNa/DpUAS.cDa/Scer\GAL4vg.int2.1/BacA\p35UAS.cHa
vg83b27 has wing phenotype, suppressible | partially by Hsap\VGLL1UAS.cVa/Scer\GAL4vg.int2.1
vg83b27 has wing phenotype, non-suppressible by Scer\GAL4vg.PU/BacA\p35UAS.cHa
Very few wgAct5C.PS; vg83b27/vg83b27 flies exhibit wg-induced transdetermination, legs with wing tissue.
Co-expression of E2fScer\UAS.cNa, DpScer\UAS.cDa and BacA\p35Scer\UAS.cHa under the control of Scer\GAL4vg.int2.1 rescues wing appendage growth in vg83b27 flies; wing blade features such as bristles, wing veins and margin are often seen in the rescued appendages.
Partial, but significant rescue of the vg83b27 lack of wing phenotype is seen when Hsap\TDUScer\UAS.cVa is expressed under the control of Scer\GAL4vg.int2.1. The rescued wings have veins and wing margins.
Expression of BacA\p35Scer\UAS.cHa under the control of Scer\GAL4vg.PU does not suppress the vg83b27 wing phenotype.
vg83b27 is rescued by vgRpL32.PZ
vg83b27 is rescued by vgαTub84B.PZa
vg83b27 is rescued by vgΔ7-8.UAS/Scer\GAL4vg.PM
vg83b27 is rescued by vgScer\UAS.cMa/Scer\GAL4vg.PM
vg83b27 is rescued by vgUAS.cZa/Scer\GAL4vg.PU
vg83b27 is partially rescued by vgΔ1-2.UAS/Scer\GAL4vg.PM
vg83b27 is partially rescued by vgΔ1-4.UAS/Scer\GAL4vg.PM
vg83b27 is partially rescued by Scer\GAL4vg.PM/vgΔ5'-5.UAS
vg83b27 is partially rescued by vgΔ5'-6.UAS/Scer\GAL4vg.PM
vg83b27 is partially rescued by vgΔ5'-7.UAS/Scer\GAL4vg.PM
vg83b27 is not rescued by vgΔ2-8.UAS/Scer\GAL4vg.PM
vg83b27 is not rescued by Scer\GAL4vg.PM/vgΔ9-10.UAS
vg83b27 is not rescued by vgΔ4-8.UAS/Scer\GAL4vg.PM
vg83b27 is not rescued by vgΔ4-10.UAS/Scer\GAL4vg.PM
vg83b27 is not rescued by vgΔ8-9.UAS/Scer\GAL4vg.PM
vg83b27 is not rescued by Scer\GAL4vg.PM/vgΔ5'-9.UAS
vg83b27 is not rescued by vgΔ2-10.UAS/Scer\GAL4vg.PM
Induced in: Middle stage spermatid.
male.
Regulatory mutant.
"irradiation" was stated as tentative. "spontaneous" was stated as tentative.