FlyBase Allele Report: Dmel\vg[83b27]

General Information

Symbol

Dmel\vg^83b27

Species

D. melanogaster

Name

FlyBase ID

FBal0017976

Feature type

allele

Associated gene

Dmel\vg

Associated Insertion(s)

Carried in Construct

Genomic Maps

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Allele class

gain of function allele

Mutagen

Nature of the Allele

Allele class

gain of function allele

(Silber et al., 1993)

Mutagen

gamma ray

(Williams and Bell, 1988, Alexandrov and Alexandrova, 1987)

irradiation

(The Moscow Regional Drosophila melanogaster Stock Center, Dubna)

spontaneous

(The Moscow Regional Drosophila melanogaster Stock Center, Dubna)

Mutations Mapped to the Genome

Type

Location

Additional Notes

References

deletion

2R:12,887,566..12,891,420 [+]

Linked to:

EcoRI-BglII restriction fragment

Comment:

Approximate boundaries of vg^83b27, a deletion which removes most of intron 2 but does not alter the surrounding exons.

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Progenitor genotype

Cytology

Polytene chromosomes normal.

(Alexandrov and Alexandrova, 1987)

Nature of the lesion

Statement

Reference

Deletion of the vg "boundary enhancer".

(van de Bor et al., 1999)

Intronic deletion.

(Abu-Issa and Cavicchi, 1996)

Deletion of intron 2.

(Williams et al., 1994)

Deletion of a major part of intron 2.

(Silber et al., 1993)

Deletion of DNA.

(Bazin et al., 1991)

Deletes intronic regulatory element within intron 2.

(Williams et al., 1991)

Deletion of vg sequences. 5' end of the deletion maps at least 500bp from the 3' end of the upstream exon and the 3' end maps quite close to the 5' end of the downstream exon.

(Williams et al., 1990)

3kb deletion from + 5 to + 8; '+' values to right (distal); 0 coordinate assigned to insert position of vg²¹; Williams, Atkin and Bell (1990)

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

vg^83b27 mutants do not develop a wing pouch.

(Hersh et al., 2007)

Mature mutant wing discs lack the wing primordium. Homozygous clones induced in mid- to late second instar larvae are able to contribute normally to the wing pouch. However, homozygous clones induced at earlier times show a progressive decrease in their ability to do so. Homozygous clones induced during the first larval instar and given a competitive growth advantage using the Minute technique appear to be excluded from the wing pouch, forming non-wing tissue at its expense.

(Zecca and Struhl, 2007)

Mutants show no growth of wing blade tissue, although some hinge structures are still present.

(Legent et al., 2006)

Homozygotes lack halteres and wings are almost always absent.

(Coulthard et al., 2005)

Homozygous flies have virtually no wings.

(MacKay et al., 2003)

Mutant adults show an almost complete lack of wings. vg^null/vg^83b27 adults show an almost complete lack of wing structures.

(Delanoue et al., 2002)

Significant cell death is not seen in the third larval instar wing disc (similar to wild type).

(van de Bor et al., 1999)

The formation of ectopic wing-like outgrowths caused by expression of vg^Scer\UAS.cZa under the control of Scer\GAL4^ptc-559.1 is more strongly reduced in a vg^83b27 homozygous background than in a vg^83b27 heterozygous background.

(Paumard-Rigal et al., 1998)

vg^WR2 produces a vg phenotype in combination with vg^83b27.

(Simmonds and Bell, 1998)

Wing phenotype is unaffected by temperature.

(Silber et al., 1997)

Flies have knobbed wings and halteres.

(Abu-Issa and Cavicchi, 1996)

Homozygotes display high rate of resistance to fluorodeoxyuridine (FUdR), an inhibitor of thymidylate synthetase and slight resistance to aminopterin.

(Silber et al., 1993)

Not suppressed by Su(vg)B27¹, Su(vg)E4¹ or Su(vg)E6¹.

(Bazin and Silber, 1992)

Wing and haltere structures eliminated: cell death of larval imaginal discs.

(Williams et al., 1991)

Homozygotes show strong wing and haltere reductions. They are wild type with respect to the postscutellar bristle and female fertility phenotypes. Transheterozygotes with vg¹ exhibit wing bubbles and unequal wing deficiencies. The complementation behaviour is unlikely to be mediated by a transvection-like process.

(Williams et al., 1990)

Homozygous viable, but with an extreme vg phenotype.

(Williams and Bell, 1988)

Homozygotes have a vg^nw wing phenotype. Postscutellars are wild-type, and halteres are missing.

(Alexandrov and Alexandrova, 1987)

homozygous wing phenotype like that of vg^nw halteres absent postscutellar bristles present homozygous viable female-fertile?

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

vg^83b27/vg¹ has visible phenotype, enhanceable by 1038¹⁰³⁸

(Coulthard et al., 2005)

vg^83b27/vg¹ has visible phenotype, enhanceable by 836⁸³⁶

(Coulthard et al., 2005)

vg^83b27/vg¹ has visible phenotype, enhanceable by In(2LR)789

(Coulthard et al., 2005)

vg^83b27/vg¹ has visible phenotype, enhanceable by T(2;3)550

(Coulthard et al., 2005)

vg^83b27/vg¹ has visible phenotype, enhanceable by T(2;3)75

(Coulthard et al., 2005)

vg^83b27/vg¹ has visible phenotype, enhanceable by T(2;3)805

(Coulthard et al., 2005)

vg^83b27/vg¹ has visible phenotype, enhanceable by T(2;3)835

(Coulthard et al., 2005)

vg^83b27/vg¹ has visible phenotype, enhanceable by T(2;3)84

(Coulthard et al., 2005)

vg^83b27/vg¹ has visible phenotype, enhanceable by 1297¹²⁹⁷

(Coulthard et al., 2005)

vg^83b27/vg¹ has visible phenotype, enhanceable by T(2;3)88

(Coulthard et al., 2005)

vg^83b27/vg¹ has visible phenotype, enhanceable by T(2;3)B9b

(Coulthard et al., 2005)

vg^83b27/vg¹ has visible phenotype, enhanceable by T(2;3)B9c

(Coulthard et al., 2005)

vg^83b27/vg¹ has visible phenotype, enhanceable by T(2;3)No3

(Coulthard et al., 2005)

vg^83b27/vg¹ has visible phenotype, enhanceable by T(2;3)No5

(Coulthard et al., 2005)

vg^83b27/vg¹ has visible phenotype, enhanceable by T(2;3)No6

(Coulthard et al., 2005)

vg^83b27/vg¹ has visible phenotype, enhanceable by T(2;3)P1C

(Coulthard et al., 2005)

vg^83b27/vg¹ has visible phenotype, enhanceable by T(2;3)PIIA

(Coulthard et al., 2005)

vg^83b27/vg¹ has visible phenotype, enhanceable by T(2;3)PIIB

(Coulthard et al., 2005)

vg^83b27/vg¹ has visible phenotype, enhanceable by Tp(2;3)P10.C

(Coulthard et al., 2005)

vg^83b27/vg¹ has visible phenotype, enhanceable by 1577¹⁵⁷⁷

(Coulthard et al., 2005)

vg^83b27/vg¹ has visible phenotype, enhanceable by 2335²³³⁵

(Coulthard et al., 2005)

NOT Enhanced by

Statement

Reference

vg^83b27/vg¹ has visible phenotype, non-enhanceable by z^a

(Coulthard et al., 2005)

Suppressed by

Statement

Reference

vg^83b27 has visible phenotype, suppressible | partially by E2f1^UAS.cNa/Dp^UAS.cDa/Scer\GAL4^vg.int2.1/BacA\p35^UAS.cHa

(Legent et al., 2006)

vg^null/vg^83b27 has visible phenotype, suppressible | partially by ap^UAS.cMa/Scer\GAL4^vg.boundary

(Delanoue et al., 2002)

vg^83b27 has visible | recessive phenotype, suppressible | partially by Hsap\VGLL1^UAS.cVa/Scer\GAL4^vg.int2.1

(Vaudin et al., 1999)

NOT suppressed by

Statement

Reference

vg^83b27/vg¹ has visible phenotype, non-suppressible by z^a

(Coulthard et al., 2005)

vg^83b27 has visible phenotype, non-suppressible by Scer\GAL4^vg.PU/BacA\p35^UAS.cHa

(van de Bor et al., 1999)

NOT Enhancer of

Statement

Reference

vg[+]/vg^83b27 is a non-enhancer of visible phenotype of Bx³

(Delanoue et al., 2002)

NOT Suppressor of

Statement

Reference

vg[+]/vg^83b27 is a non-suppressor of visible phenotype of Bx³

(Delanoue et al., 2002)

Phenotype Manifest In

Enhanced by

Statement

Reference

vg^83b27/vg¹ has haltere phenotype, enhanceable by 1038¹⁰³⁸

(Coulthard et al., 2005)

vg^83b27/vg¹ has haltere phenotype, enhanceable by 2335²³³⁵

(Coulthard et al., 2005)

vg^83b27/vg¹ has wing phenotype, enhanceable by 2335²³³⁵

(Coulthard et al., 2005)

vg^83b27/vg¹ has haltere phenotype, enhanceable by 836⁸³⁶

(Coulthard et al., 2005)

vg^83b27/vg¹ has wing phenotype, enhanceable by 836⁸³⁶

(Coulthard et al., 2005)

vg^83b27/vg¹ has haltere phenotype, enhanceable by In(2LR)789

(Coulthard et al., 2005)

vg^83b27/vg¹ has wing phenotype, enhanceable by In(2LR)789

(Coulthard et al., 2005)

vg^83b27/vg¹ has haltere phenotype, enhanceable by T(2;3)550

(Coulthard et al., 2005)

vg^83b27/vg¹ has wing phenotype, enhanceable by 1038¹⁰³⁸

(Coulthard et al., 2005)

vg^83b27/vg¹ has wing phenotype, enhanceable by T(2;3)550

(Coulthard et al., 2005)

vg^83b27/vg¹ has haltere phenotype, enhanceable by T(2;3)75

(Coulthard et al., 2005)

vg^83b27/vg¹ has haltere phenotype, enhanceable by T(2;3)805

(Coulthard et al., 2005)

vg^83b27/vg¹ has wing phenotype, enhanceable by T(2;3)805

(Coulthard et al., 2005)

vg^83b27/vg¹ has haltere phenotype, enhanceable by T(2;3)835

(Coulthard et al., 2005)

vg^83b27/vg¹ has wing phenotype, enhanceable by T(2;3)835

(Coulthard et al., 2005)

vg^83b27/vg¹ has wing phenotype, enhanceable by T(2;3)84

(Coulthard et al., 2005)

vg^83b27/vg¹ has haltere phenotype, enhanceable by T(2;3)88

(Coulthard et al., 2005)

vg^83b27/vg¹ has wing phenotype, enhanceable by T(2;3)88

(Coulthard et al., 2005)

vg^83b27/vg¹ has haltere phenotype, enhanceable by T(2;3)B9b

(Coulthard et al., 2005)

vg^83b27/vg¹ has wing phenotype, enhanceable by T(2;3)B9b

(Coulthard et al., 2005)

vg^83b27/vg¹ has haltere phenotype, enhanceable by T(2;3)B9c

(Coulthard et al., 2005)

vg^83b27/vg¹ has wing phenotype, enhanceable by T(2;3)B9c

(Coulthard et al., 2005)

vg^83b27/vg¹ has haltere phenotype, enhanceable by 1297¹²⁹⁷

(Coulthard et al., 2005)

vg^83b27/vg¹ has haltere phenotype, enhanceable by T(2;3)No3

(Coulthard et al., 2005)

vg^83b27/vg¹ has wing phenotype, enhanceable by T(2;3)No3

(Coulthard et al., 2005)

vg^83b27/vg¹ has haltere phenotype, enhanceable by T(2;3)No5

(Coulthard et al., 2005)

vg^83b27/vg¹ has wing phenotype, enhanceable by T(2;3)No5

(Coulthard et al., 2005)

vg^83b27/vg¹ has haltere phenotype, enhanceable by T(2;3)No6

(Coulthard et al., 2005)

vg^83b27/vg¹ has wing phenotype, enhanceable by T(2;3)No6

(Coulthard et al., 2005)

vg^83b27/vg¹ has wing phenotype, enhanceable by 1297¹²⁹⁷

(Coulthard et al., 2005)

vg^83b27/vg¹ has haltere phenotype, enhanceable by T(2;3)P1C

(Coulthard et al., 2005)

vg^83b27/vg¹ has wing phenotype, enhanceable by T(2;3)P1C

(Coulthard et al., 2005)

vg^83b27/vg¹ has haltere phenotype, enhanceable by T(2;3)PIIA

(Coulthard et al., 2005)

vg^83b27/vg¹ has wing phenotype, enhanceable by T(2;3)PIIA

(Coulthard et al., 2005)

vg^83b27/vg¹ has haltere phenotype, enhanceable by T(2;3)PIIB

(Coulthard et al., 2005)

vg^83b27/vg¹ has haltere phenotype, enhanceable by Tp(2;3)P10.C

(Coulthard et al., 2005)

vg^83b27/vg¹ has wing phenotype, enhanceable by Tp(2;3)P10.C

(Coulthard et al., 2005)

vg^83b27/vg¹ has haltere phenotype, enhanceable by 1577¹⁵⁷⁷

(Coulthard et al., 2005)

vg^83b27/vg¹ has wing phenotype, enhanceable by 1577¹⁵⁷⁷

(Coulthard et al., 2005)

NOT Enhanced by

Statement

Reference

vg^83b27/vg¹ has wing phenotype, non-enhanceable by T(2;3)PIIB

(Coulthard et al., 2005)

vg^83b27/vg¹ has wing phenotype, non-enhanceable by z^a

(Coulthard et al., 2005)

Suppressed by

Statement

Reference

vg^83b27 has wing blade phenotype, suppressible | partially by E2f1^UAS.cNa/Dp^UAS.cDa/Scer\GAL4^vg.int2.1/BacA\p35^UAS.cHa

(Legent et al., 2006)

vg^null/vg^83b27 has wing phenotype, suppressible | partially by ap^UAS.cMa/Scer\GAL4^vg.boundary

(Delanoue et al., 2002)

vg^83b27 has wing phenotype, suppressible | partially by Hsap\VGLL1^UAS.cVa/Scer\GAL4^vg.int2.1

(Vaudin et al., 1999)

NOT suppressed by

Statement

Reference

vg^83b27/vg¹ has wing phenotype, non-suppressible by z^a

(Coulthard et al., 2005)

vg^83b27 has wing phenotype, non-suppressible by Scer\GAL4^vg.PU/BacA\p35^UAS.cHa

(van de Bor et al., 1999)

NOT Enhancer of

Statement

Reference

vg[+]/vg^83b27 is a non-enhancer of wing phenotype of Bx³

(Delanoue et al., 2002)

NOT Suppressor of

Statement

Reference

vg[+]/vg^83b27 is a non-suppressor of wing phenotype of Bx³

(Delanoue et al., 2002)

Additional Comments

Genetic Interactions

Statement

Reference

z^a has no effect on the complementation seen between vg¹ and vg^83b27.

(Coulthard et al., 2005)

The scalloped wing phenotype seen in Bx³ homozygotes is unaffected by vg^83b27/+.

(Delanoue et al., 2002)

Very few wg^Act5C.PS; vg^83b27/vg^83b27 flies exhibit wg-induced transdetermination, legs with wing tissue.

(Maves and Schubiger, 1998)

Xenogenetic Interactions

Statement

Reference

Co-expression of E2f^Scer\UAS.cNa, Dp^Scer\UAS.cDa and BacA\p35^Scer\UAS.cHa under the control of Scer\GAL4^vg.int2.1 rescues wing appendage growth in vg^83b27 flies; wing blade features such as bristles, wing veins and margin are often seen in the rescued appendages.

(Legent et al., 2006)

Partial, but significant rescue of the vg^83b27 lack of wing phenotype is seen when Hsap\TDU^Scer\UAS.cVa is expressed under the control of Scer\GAL4^vg.int2.1. The rescued wings have veins and wing margins.

(Vaudin et al., 1999)

Expression of BacA\p35^Scer\UAS.cHa under the control of Scer\GAL4^vg.PU does not suppress the vg^83b27 wing phenotype.

(van de Bor et al., 1999)

Complementation and Rescue Data

Partially complements

vg¹

(Coulthard et al., 2005)

vg¹²

(Williams et al., 1990)

vg^nw

(Williams et al., 1990)

Rescued by

vg^83b27 is rescued by vg^RpL32.PZ

(Zecca and Struhl, 2007)

vg^83b27 is rescued by vg^αTub84B.PZa

(Zecca and Struhl, 2007)

vg^83b27 is rescued by vg^Δ7-8.UAS/Scer\GAL4^vg.PM

(MacKay et al., 2003)

vg^83b27 is rescued by vg^Scer\UAS.cMa/Scer\GAL4^vg.PM

(MacKay et al., 2003)

vg^83b27 is rescued by vg^UAS.cZa/Scer\GAL4^vg.PU

(van de Bor et al., 1999)

Partially rescued by

vg^83b27 is partially rescued by vg^Δ1-2.UAS/Scer\GAL4^vg.PM

(MacKay et al., 2003)

vg^83b27 is partially rescued by vg^Δ1-4.UAS/Scer\GAL4^vg.PM

(MacKay et al., 2003)

vg^83b27 is partially rescued by Scer\GAL4^vg.PM/vg^Δ5'-5.UAS

(MacKay et al., 2003)

vg^83b27 is partially rescued by vg^Δ5'-6.UAS/Scer\GAL4^vg.PM

(MacKay et al., 2003)

vg^83b27 is partially rescued by vg^Δ5'-7.UAS/Scer\GAL4^vg.PM

(MacKay et al., 2003)

Not rescued by

vg^83b27 is not rescued by vg^Δ2-8.UAS/Scer\GAL4^vg.PM

(MacKay et al., 2003)

vg^83b27 is not rescued by Scer\GAL4^vg.PM/vg^Δ9-10.UAS

(MacKay et al., 2003)

vg^83b27 is not rescued by vg^Δ4-8.UAS/Scer\GAL4^vg.PM

(MacKay et al., 2003)

vg^83b27 is not rescued by vg^Δ4-10.UAS/Scer\GAL4^vg.PM

(MacKay et al., 2003)

vg^83b27 is not rescued by vg^Δ8-9.UAS/Scer\GAL4^vg.PM

(MacKay et al., 2003)

vg^83b27 is not rescued by Scer\GAL4^vg.PM/vg^Δ5'-9.UAS

(MacKay et al., 2003)

vg^83b27 is not rescued by vg^Δ2-10.UAS/Scer\GAL4^vg.PM

(MacKay et al., 2003)

Comments

vg¹/vg^83b27 transheterozygotes have less severe wing defects than either homozygote and halteres are usually present in the transheterozygotes (they are absent in either homozygote).

(Coulthard et al., 2005)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Induced in: Middle stage spermatid.

(Alexandrov and Alexandrova, 1987)

Comments

male.

(Alexandrov and Alexandrova, 1987)

Regulatory mutant.

(Williams et al., 1991)

"irradiation" was stated as tentative. "spontaneous" was stated as tentative.

(The Moscow Regional Drosophila melanogaster Stock Center, Dubna)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

vg^83b27

(Hersh et al., 2007, Zecca and Struhl, 2007, Zecca and Struhl, 2007, Legent et al., 2006, Delanoue et al., 2002, van de Bor et al., 1999)

vg^b

(Zecca and Struhl, 2007)

Name Synonyms

Secondary FlyBase IDs

References (30)

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