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General Information
Symbol
Dmel\vg83b27
Species
D. melanogaster
Name
FlyBase ID
FBal0017976
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
deletion
Linked to:
EcoRI-BglII restriction fragment
Comment:

Approximate boundaries of vg83b27, a deletion which removes most of intron 2 but does not alter the surrounding exons.

Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology

Polytene chromosomes normal.

Nature of the lesion
Statement
Reference

Deletion of the vg "boundary enhancer".

Intronic deletion.

Deletion of intron 2.

Deletion of a major part of intron 2.

Deletion of DNA.

Deletes intronic regulatory element within intron 2.

Deletion of vg sequences. 5' end of the deletion maps at least 500bp from the 3' end of the upstream exon and the 3' end maps quite close to the 5' end of the downstream exon.

3kb deletion from + 5 to + 8; '+' values to right (distal); 0 coordinate assigned to insert position of vg21; Williams, Atkin and Bell (1990)

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

vg83b27 mutants do not develop a wing pouch.

Mature mutant wing discs lack the wing primordium.

Homozygous clones induced in mid- to late second instar larvae are able to contribute normally to the wing pouch. However, homozygous clones induced at earlier times show a progressive decrease in their ability to do so. Homozygous clones induced during the first larval instar and given a competitive growth advantage using the Minute technique appear to be excluded from the wing pouch, forming non-wing tissue at its expense.

Mutants show no growth of wing blade tissue, although some hinge structures are still present.

Homozygotes lack halteres and wings are almost always absent.

Homozygous flies have virtually no wings.

Mutant adults show an almost complete lack of wings.

vgnull/vg83b27 adults show an almost complete lack of wing structures.

Significant cell death is not seen in the third larval instar wing disc (similar to wild type).

The formation of ectopic wing-like outgrowths caused by expression of vgScer\UAS.cZa under the control of Scer\GAL4ptc-559.1 is more strongly reduced in a vg83b27 homozygous background than in a vg83b27 heterozygous background.

vgWR2 produces a vg phenotype in combination with vg83b27.

Wing phenotype is unaffected by temperature.

Flies have knobbed wings and halteres.

Homozygotes display high rate of resistance to fluorodeoxyuridine (FUdR), an inhibitor of thymidylate synthetase and slight resistance to aminopterin.

Wing and haltere structures eliminated: cell death of larval imaginal discs.

Homozygotes show strong wing and haltere reductions. They are wild type with respect to the postscutellar bristle and female fertility phenotypes. Transheterozygotes with vg1 exhibit wing bubbles and unequal wing deficiencies. The complementation behaviour is unlikely to be mediated by a transvection-like process.

Homozygous viable, but with an extreme vg phenotype.

Homozygotes have a vgnw wing phenotype. Postscutellars are wild-type, and halteres are missing.

homozygous wing phenotype like that of vgnw halteres absent postscutellar bristles present homozygous viable female-fertile?

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement
Reference

vg83b27/vg1 has visible phenotype, enhanceable by 10381038

vg83b27/vg1 has visible phenotype, enhanceable by 836836

vg83b27/vg1 has visible phenotype, enhanceable by In(2LR)789

vg83b27/vg1 has visible phenotype, enhanceable by T(2;3)550

vg83b27/vg1 has visible phenotype, enhanceable by T(2;3)75

vg83b27/vg1 has visible phenotype, enhanceable by T(2;3)805

vg83b27/vg1 has visible phenotype, enhanceable by T(2;3)835

vg83b27/vg1 has visible phenotype, enhanceable by T(2;3)84

vg83b27/vg1 has visible phenotype, enhanceable by 12971297

vg83b27/vg1 has visible phenotype, enhanceable by T(2;3)88

vg83b27/vg1 has visible phenotype, enhanceable by T(2;3)B9b

vg83b27/vg1 has visible phenotype, enhanceable by T(2;3)B9c

vg83b27/vg1 has visible phenotype, enhanceable by T(2;3)No3

vg83b27/vg1 has visible phenotype, enhanceable by T(2;3)No5

vg83b27/vg1 has visible phenotype, enhanceable by T(2;3)No6

vg83b27/vg1 has visible phenotype, enhanceable by T(2;3)P1C

vg83b27/vg1 has visible phenotype, enhanceable by T(2;3)PIIA

vg83b27/vg1 has visible phenotype, enhanceable by T(2;3)PIIB

vg83b27/vg1 has visible phenotype, enhanceable by Tp(2;3)P10.C

vg83b27/vg1 has visible phenotype, enhanceable by 15771577

vg83b27/vg1 has visible phenotype, enhanceable by 23352335

NOT Enhanced by
Statement
Reference

vg83b27/vg1 has visible phenotype, non-enhanceable by za

Suppressed by
NOT suppressed by
Statement
Reference

vg83b27/vg1 has visible phenotype, non-suppressible by za

NOT Enhancer of
Statement
Reference

vg[+]/vg83b27 is a non-enhancer of visible phenotype of Bx3

NOT Suppressor of
Statement
Reference

vg[+]/vg83b27 is a non-suppressor of visible phenotype of Bx3

Phenotype Manifest In
Enhanced by
Statement
Reference

vg83b27/vg1 has haltere phenotype, enhanceable by 10381038

vg83b27/vg1 has haltere phenotype, enhanceable by 23352335

vg83b27/vg1 has wing phenotype, enhanceable by 23352335

vg83b27/vg1 has haltere phenotype, enhanceable by 836836

vg83b27/vg1 has wing phenotype, enhanceable by 836836

vg83b27/vg1 has haltere phenotype, enhanceable by In(2LR)789

vg83b27/vg1 has wing phenotype, enhanceable by In(2LR)789

vg83b27/vg1 has haltere phenotype, enhanceable by T(2;3)550

vg83b27/vg1 has wing phenotype, enhanceable by 10381038

vg83b27/vg1 has wing phenotype, enhanceable by T(2;3)550

vg83b27/vg1 has haltere phenotype, enhanceable by T(2;3)75

vg83b27/vg1 has haltere phenotype, enhanceable by T(2;3)805

vg83b27/vg1 has wing phenotype, enhanceable by T(2;3)805

vg83b27/vg1 has haltere phenotype, enhanceable by T(2;3)835

vg83b27/vg1 has wing phenotype, enhanceable by T(2;3)835

vg83b27/vg1 has wing phenotype, enhanceable by T(2;3)84

vg83b27/vg1 has haltere phenotype, enhanceable by T(2;3)88

vg83b27/vg1 has wing phenotype, enhanceable by T(2;3)88

vg83b27/vg1 has haltere phenotype, enhanceable by T(2;3)B9b

vg83b27/vg1 has wing phenotype, enhanceable by T(2;3)B9b

vg83b27/vg1 has haltere phenotype, enhanceable by T(2;3)B9c

vg83b27/vg1 has wing phenotype, enhanceable by T(2;3)B9c

vg83b27/vg1 has haltere phenotype, enhanceable by 12971297

vg83b27/vg1 has haltere phenotype, enhanceable by T(2;3)No3

vg83b27/vg1 has wing phenotype, enhanceable by T(2;3)No3

vg83b27/vg1 has haltere phenotype, enhanceable by T(2;3)No5

vg83b27/vg1 has wing phenotype, enhanceable by T(2;3)No5

vg83b27/vg1 has haltere phenotype, enhanceable by T(2;3)No6

vg83b27/vg1 has wing phenotype, enhanceable by T(2;3)No6

vg83b27/vg1 has wing phenotype, enhanceable by 12971297

vg83b27/vg1 has haltere phenotype, enhanceable by T(2;3)P1C

vg83b27/vg1 has wing phenotype, enhanceable by T(2;3)P1C

vg83b27/vg1 has haltere phenotype, enhanceable by T(2;3)PIIA

vg83b27/vg1 has wing phenotype, enhanceable by T(2;3)PIIA

vg83b27/vg1 has haltere phenotype, enhanceable by T(2;3)PIIB

vg83b27/vg1 has haltere phenotype, enhanceable by Tp(2;3)P10.C

vg83b27/vg1 has wing phenotype, enhanceable by Tp(2;3)P10.C

vg83b27/vg1 has haltere phenotype, enhanceable by 15771577

vg83b27/vg1 has wing phenotype, enhanceable by 15771577

NOT Enhanced by
Statement
Reference

vg83b27/vg1 has wing phenotype, non-enhanceable by T(2;3)PIIB

vg83b27/vg1 has wing phenotype, non-enhanceable by za

Suppressed by
NOT suppressed by
Statement
Reference

vg83b27/vg1 has wing phenotype, non-suppressible by za

NOT Enhancer of
Statement
Reference

vg[+]/vg83b27 is a non-enhancer of wing phenotype of Bx3

NOT Suppressor of
Statement
Reference

vg[+]/vg83b27 is a non-suppressor of wing phenotype of Bx3

Additional Comments
Genetic Interactions
Statement
Reference

za has no effect on the complementation seen between vg1 and vg83b27.

The scalloped wing phenotype seen in Bx3 homozygotes is unaffected by vg83b27/+.

Very few wgAct5C.PS; vg83b27/vg83b27 flies exhibit wg-induced transdetermination, legs with wing tissue.

Xenogenetic Interactions
Statement
Reference

Co-expression of E2fScer\UAS.cNa, DpScer\UAS.cDa and BacA\p35Scer\UAS.cHa under the control of Scer\GAL4vg.int2.1 rescues wing appendage growth in vg83b27 flies; wing blade features such as bristles, wing veins and margin are often seen in the rescued appendages.

Partial, but significant rescue of the vg83b27 lack of wing phenotype is seen when Hsap\TDUScer\UAS.cVa is expressed under the control of Scer\GAL4vg.int2.1. The rescued wings have veins and wing margins.

Expression of BacA\p35Scer\UAS.cHa under the control of Scer\GAL4vg.PU does not suppress the vg83b27 wing phenotype.

Complementation and Rescue Data
Comments

vg1/vg83b27 transheterozygotes have less severe wing defects than either homozygote and halteres are usually present in the transheterozygotes (they are absent in either homozygote).

Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer

Induced in: Middle stage spermatid.

Comments
Comments

Regulatory mutant.

"irradiation" was stated as tentative. "spontaneous" was stated as tentative.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
References (30)