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General Information
Symbol
Dmel\vls1
Species
D. melanogaster
Name
FlyBase ID
FBal0018013
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
vlsRB71, vlsRB
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
point mutation
Nucleotide change:

G20064859A

Reported nucleotide change:

G?A

Amino acid change:

W228term | vls-PA

Reported amino acid change:

W228term

Comment:

TGG to TAG

Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

Nucleotide substitution: G?A.

Amino acid replacement: W228term.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

The karyosome is fragmented into two fuzzy spots in mutant embryos, instead of forming a single compact dot as occurs in wild-type nuclei.

vls1;6xP{osk+108} embryos exhibit a vls phenotype.

Does not interact with RpII140wimp maternal effect.

Homozygous vls1 embryos derived from homozygous females have no polar granules, fail to form pole cells and have deletions of abdominal structures. vas and CycB transcripts are localized at the posterior.

Eggs derived from homozygous females form a syncytial blastoderm but 80-90% of the embryos fail to cellularise. Those that do cellularise show variable cellularisation defects. The embryos show a "grandchildless-knirps" phenotype; they lack polar granules and pole cells and show typical maternal kni-like abdominal segment deletions.

maternal-effect lethal Embryos from homozygous mothers exhibit a so-called "grandchildless-knirps" phenotype: all eggs lack polar granules and no pole cells are formed; most of the embryos show variable deletions of abdominal segments; whereby segment A4 is deleted most frequently, larger deletions may remove segments A2 through A7; in extreme cases anterior parts of segment A1 become fused to posterior parts of segment A8, but telson elements are always present and relatively normal. In addition, 80-90% of the embryos fail to cellularize normally at the blastoderm stage and die without forming cuticle, or only fragmented pieces of cuticle. Analysis of germ-line clones indicates that the mutation is germ-line autonomous (Schupbach and Wieschaus, 1986).

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference

The formation of ectopic anterior pole cells seen in oskbcd.UTR embryos is suppressed in vls3 blastoderm embryos.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (3)
Notes on Origin
Discoverer
Comments
Comments

Although tud protein is present in mutant embryo extracts, its localization in the embryo is altered.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (6)
References (23)