Prevents the posterior accumulation of G-iα65A protein.
Absence of posterior pole plasm, polar granules and pole cells.
Embryos lack pole cells. Large amounts of vas protein are expressed in early stages of oogenesis. vas protein localizes to the posterior pole at the same stage of egg development as wild type, the protein disappears by early gastrulation.
Hemizygous vls2embryos derived from homozygous females have no polar granules, fail to form pole cells and have deletions of abdominal structures. vas and CycB transcripts are localized at the posterior.
Eggs derived from homozygous females form a syncytial blastoderm but 80-90% of the embryos fail to cellularise. Those that do cellularise show variable cellularisation defects. The embryos show a "grandchildless-knirps" phenotype; they lack polar granules and pole cells and show typical maternal kni-like abdominal segment deletions.
Homozygous females produce embryos that fail to form pole cells, lack polar granules normally found at the posterior pole, and have deletions of abdominal segments. There are also frequent defects in cellularisation at the blastoderm stage.
Embryos derived from mutant females completely lack pole cells and show deletions in the abdominal segments. The same phenotype is seen in embryos derived from homozygous or hemizygous females.