FB2025_01 , released February 20, 2025
Allele: Dmel\wap2
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General Information
Symbol
Dmel\wap2
Species
D. melanogaster
Name
FlyBase ID
FBal0018410
Feature type
allele
Associated gene
Dmel\wap
Associated Insertion(s)
Carried in Construct
Also Known As
Q217, wapQ217
Key Links
Genomic Maps

Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
ethyl methanesulfonate
(Schalet and Lefevre, 1973)
Progenitor genotype
Cytology
Description

Amino acid replacement: ?319term.

(Morriss et al., 2013)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
point_mutation
X:22,453,307..22,453,307 [-]
Nucleotide change:

C22453307T

Amino acid change:

Q319term | wap-PA; Q319term | wap-PB; Q319term | wap-PC; Q319term | wap-PD

Reported amino acid change:

?319term

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

(Morriss et al., 2013)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      wap2/wap9 mutant flies are characterised by three phenotypes: 1) In ~10% of adult flies there are supernumerary crossveins located between the second and third longitudinal wing veins 2) the tergal depressor of the trochanter (TDT) (a prominent tubular jump muscle in the thorax) is missing 3) mutants show semi-lethality.

      wap2 mutants initiate founder cell specification and myoblast fusion of the tergal depressor of the trochanter (TDT) jump muscle, but this is not sustained and the nascent jump muscles don't continue development.

      wap2/Df(1)BSC708 mutant flies are viable and have normal vein vein patterning and an intact tergal depressor of the trochanter (TDT) jump muscle.

      wap2/Df(1)LB6 mutant flies are viable and have normal vein vein patterning and an intact tergal depressor of the trochanter (TDT) jump muscle.

      wap2/Df(1)R8A mutant flies are viable and have normal vein vein patterning and an intact tergal depressor of the trochanter (TDT) jump muscle.

      wap2/Df(1)Exel6255 mutant flies are semi-lethal and exhibit an additional crossvein between the second and third longitudinal vein. The jump muscle is absent in almost all flies. In cases where the muscle is present, it exhibits highly abnormal morphology and a >60% reduction in the number of fibres compared to wild type.

      (Morriss et al., 2013)

      Lethality occurs during pupal or adult stages. Homozygous germline clones display no maternal effect. Embryos derived from heterozygous mothers exhibit no CNS or PNS defects.

      (Perrimon et al., 1989)

      Most flies die just before eclosion, those which do hatch usually have their wings slightly set apart, a somewhat darker than normal thorax and one or more extra crossveins. Hemizygous females have the same phenotype as hemizygous males and homozygous females. Approximately 50% of the expected number of wap2/Df(1)su(f)5 females survive at 24-25oC, whereas only about 1% hatch at 29-30oC. The mutant phenotype is more extreme at the higher temperature.

      (Schalet, 1972)

      no maternal effect in homozygous germ-line clones

      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Fails to complement

      wap1

      (Schalet and Lefevre, 1973)
      Partially rescued by

      wap2 is partially rescued by wap+tMa

      (Morriss et al., 2013)
      Comments

      Expression of wap+tMa partially rescues the phenotypes seen in wap2/Y mutant flies. The pupal lethality is not rescued, but the loss of the jump muscle is rescued, although it is still reduced in size compared to wild type.

      (Morriss et al., 2013)
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer

      Lifschytz.

      Comments
      Comments

      Maternal germline clonal analysis demonstrates there is no maternal effect.

      (Perrimon et al., 1989)
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (4)
      References (9)