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General Information
Symbol
Dmel\wapl2
Species
D. melanogaster
Name
FlyBase ID
FBal0018419
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
waplC204, l(1)C204, C204
Mutagen
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

970bp insertion of DNA from the Egfr gene into an exon of wapl that is common to both the large and small proteins eliminates the production of both protein isoforms.

Insertion of approximately 1.6kb DNA into wapl.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Mutants show a significant amount of chromatid stretching during mitotic anaphase compared to wild type.

Embryos derived from germline clones usually arrest prior to blastoderm formation but manage to form small patches of cuticle. Brain cells of mutant larvae show defects in heterochromatin organisation. In metaphase figures, the sister chromatids of the major autosomes and of the X chromosome are aligned parallel to each other, instead of the wild-type X- and V- shaped arrangements. The Y and 4th chromosomes are resolved into two distinct sister chromatids, in contrast to wild type. The sister chromatids of all mutant chromosomes are not completely separated but are instead connected by a thread located in the centromeric region. The mitotic index is normal. wapl2/In(1)FM7 females show a significant increase in X chromosome nondisjunction compared to control +/In(1)FM7 females. wapl2/+ females show the same level of X chromosome nondisjunction as control +/+ females.

Transmission rate of Dp(1;f)J21A through females to progeny is 28%, wapl mutation has no effect on transmission.

Lethality occurs during larval and pupal stages. Phenotype of homozygous germ line clones is maternal effect lethal.

L3 larval/pupal boundary lethal exhibit small disk phenotype; homozygous germ-line clones produce zygotes that arrest in preblastoderm stage.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
NOT suppressed by
Statement
Reference

wapl2 has phenotype, non-suppressible by Cyp4d1unspecified

Additional Comments
Genetic Interactions
Statement
Reference

Mutant phenotype cannot be rescued by P element mediated transformation of a wild type copy of Cyp4d1.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Rescued by
Comments

The lethality of wapl2 is not rescued by expression of either waplScer\UAS.S or waplScer\UAS.L under the control of Scer\GAL4arm.PS.

Images (0)
Mutant
Wild-type
Stocks (2)
Notes on Origin
Discoverer

Lefevre.

Comments
Comments

Maternal germline clonal analysis demonstrates there is a maternal effect lethal phenotype, preblastoderm defect.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (5)
References (11)