Open Close
General Information
Symbol
Dmel\wgl-8
Species
D. melanogaster
Name
FlyBase ID
FBal0018500
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
wgIG22, wg1-8, wgIG
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Larval muscle 4 neuromuscular junctions in wgl-8 heterozygotes do not show significant changes in synaptic bouton number, as compared to controls.

wgl-8 heterozygosity does not significantly affect the mitotic index in the adult midgut epithelium, as compared to controls.

wgl-8 embryos show segment polarity phenotype and no naked cuticle between denticle belts on the ventral epidermis (resulting in a lawn of denticles).

All epidermal cells secrete denticles in the mutant embryo.

wgl-8 mutant embryos have no second midgut constriction.

Embryos completely lack segment polarity and have a lawn of uniform, large, thick denticles covering the ventral epidermis. The embryos are much smaller than wild type. They are usually closed dorsally. There are only 8 rows of ventral epidermal cells per segment in wgl-8 embryos (wild-type number is, on average, 12 rows per segment). The cells are larger than normal and are cuboidal. Cells in the ventral epidermis enter mitosis 16 in mutant embryos (as occurs in wild type) and mitotic figures are as readily apparent in the ventral epidermis in stage 12 mutant embryos as they are in wild-type embryos.

Patterning defects evident in the denticle belts. Many mutant embryos are dorsally closed but exhibit severe defects in dorsal patterning, with loss of dorsal hairs and the presence of abnormal cuticular structures. A proportion show a mild dorsal-open phenotype. Leading edge cells never elongate along the DV axis, instead they stretch along the AP axis. Certain lateral cells contract like leading edge cells, stretching along the AP axis.

Posterior cells of each segment are transformed to anterior fates in homozygous embryos and secrete denticles. Most of the denticles of these embryos resemble the large type found in normal row 5. Denticle orientation is often reversed or aligned towards the ventral midline.

Homozygous embryos lack RP2 neurons.

Embryos are shorter than wild-type and secrete only denticles with no naked cuticle. wgl-8 embryos expressing armS10.Scer\UAS.T:Hsap\MYC (using Scer\GAL4e22c) produce a cuticle phenotype similar to embryos expressing armS10.Scer\UAS.T:Hsap\MYC (using Scer\GAL4e22c) in a wild-type background; nearly all secreted cuticle is naked. wgl-8 embryos expressing armΔN.Scer\UAS (using Scer\GAL4e22c) secrete naked cuticle interspersed with denticles on their ventral surface, while the dorsal surface is unchanged from the wgl-8 mutant phenotype.

Only one central invagination fold of the stomodeal invagination is observed.

Embryos exhibit a lawn of denticle belts.

No reduction in rate of germ band extension, in wg, ptc, en triple mutants.

Similar mutant leg phenotypes, e.g. duplications and bifurcations, are produced by dsh clones, dsh, wg double heterozygotes and wg mutants. There is allele specificity in these interactions.

Defective in gonad assembly.

Mutant embryos have a lawn of row-5-type ventral denticles, with segmental polarity reversals.

hh expression in the embryo begins to fade as the germ band reaches full extension, though it does persist in a few midline cells which may be neuroblasts.

Before stage 10 wg embryos have normal hh expression, but it begins to decay at stage 10 and is gone by stage 11.

Very strong segment polarity phenotype.

wgl-8 and wgl-17 embryos show no localized increases of arm protein in the epidermis at the time the stripes would be apparent in wild type embryos, but increases were seen in domains that do not express wg.

The RP2 neuron is the only identifiable cell altered. The restricted phenotype may be due to improper communication between neurons.

Embryos lack head cuticle and there are no or very rudimentary filzkorper. Embryos show an aberrant pattern of cell death and lack of parasegmental and segmental boundaries.

embryonic lethal

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
NOT Enhanced by
Suppressed by
Statement
Reference
NOT suppressed by
Enhancer of
Statement
Reference

wg[+]/wgl-8 is an enhancer of visible | recessive phenotype of dally06464

NOT Enhancer of
Statement
Reference

wgl-8 is a non-enhancer of visible phenotype of upd1GMR.PB

wg[+]/wgl-8 is a non-enhancer of visible | heat sensitive phenotype of fzI.hs

Suppressor of
Statement
Reference

wg[+]/wgl-8 is a suppressor | partially of visible phenotype of Scer\GAL4pnr-MD237, hepUAS.cBa

wg[+]/wgl-8 is a suppressor of increased cell number | female | adult stage | heat sensitive phenotype of Df(2R)BSC132/Mmp2Y53N

wg[+]/wgl-8 is a suppressor of neuroanatomy defective phenotype of spin10403

NOT Suppressor of
Statement
Reference

wgl-8 is a non-suppressor of visible phenotype of upd1GMR.PB

wg[+]/wgl-8 is a non-suppressor of visible | heat sensitive phenotype of fzI.hs

Phenotype Manifest In
Enhanced by
Statement
Reference
NOT Enhanced by
Suppressed by
Statement
Reference

wgl-8 has RP2sib neuron phenotype, suppressible by slp1hs.PC

wgl-8 has neuroblast NB4-2 phenotype, suppressible by slp1hs.PC

wgl-8 has RP2 motor neuron phenotype, suppressible by slp1hs.PC

wgl-8 has RP2 motor neuron phenotype, suppressible by slp2hs.PC

NOT suppressed by
Enhancer of
Statement
Reference

wg[+]/wgl-8 is an enhancer of wing phenotype of dally06464

wg[+]/wgl-8 is an enhancer of wing margin bristle phenotype of dally06464

NOT Enhancer of
Statement
Reference

wgl-8 is a non-enhancer of eye phenotype of upd1GMR.PB

wg[+]/wgl-8 is a non-enhancer of wing hair | supernumerary phenotype of fzI.hs

Suppressor of
Statement
Reference

wg[+]/wgl-8 is a suppressor | partially of scutellum phenotype of Scer\GAL4pnr-MD237, hepUAS.cBa

wg[+]/wgl-8 is a suppressor of stalk follicle cell | supernumerary | heat sensitive phenotype of Df(2R)BSC132/Mmp2Y53N

wg[+]/wgl-8 is a suppressor of stalk follicle cell | supernumerary | heat sensitive phenotype of Df(2R)BSC132/Mmp2Y675N

wg[+]/wgl-8 is a suppressor of eye disc phenotype of spin10403

wg[+]/wgl-8 is a suppressor of glial cell phenotype of spin10403

NOT Suppressor of
Statement
Reference

wg[+]/wgl-8 is a non-suppressor of NMJ bouton | larval stage phenotype of DAAMEx68

wg[+]/wgl-8 is a non-suppressor of LL1 motor neuron | larval stage phenotype of DAAMEx68

wgl-8 is a non-suppressor of arista | supernumerary phenotype of obk1

wgl-8 is a non-suppressor of eye phenotype of upd1GMR.PB

wg[+]/wgl-8 is a non-suppressor of wing hair | supernumerary phenotype of fzI.hs

Other
Statement
Reference
Additional Comments
Genetic Interactions
Statement
Reference

wgl-8 heterozygosity does not suppress the increased mitotic index in the adult midgut epithelium induced by the adulthood-only expression of UvragHMS01357 under the control of Scer\GAL4esg-NP5130 (and Gal80[ts], for the temporal control of expression).

The segment polarity phenotype and the lack of naked cuticle between denticle belts on the ventral epidermis characteristic for zygotically mutant wgl-8 embryos is not modified by Scer\GAL4arm.PU-driven expression of either sggScer\UAS.T:Ivir\HA1 or sggKK-MI.Scer\UAS.T:Myr-Src64B,T:Ivir\HA1.

The naked cuticle phenotype (loss of denticle belts on the ventral epidermis) observed in embryos expressing sggScer\UAS.T:Myr-Src64B,T:Ivir\HA1 under the control of Scer\GAL4arm.PU is not affected by combination with wgl-8 homozygosity (which on its own leads to loss of naked cuticle and ectopic denticles).

he increase in average cell number in the stalks between egg chambers which is seen in Mmp2Y53N/Df(2R)BSC132 females after being shifted to the restrictive temperature is dominantly suppressed if the females also carry a single copy of wgl-8.

ovosvb-108/Y ; wgl-8/+ larvae raised at 25[o]C have significantly fewer quaternary trichomes compared to controls.

Loss of the second midgut constriction in wgl-8 mutant embryos is suppressed by CbydsRNA.cTa. Loss of the first midgut constriction in CbydsRNA.cTa injected embryos is partially suppressed by wgl-8.

Expression of slp1hs.PC in wgl-8 embryos rescues the RP2/sib lineage in as many as 85% of hemisegments. Rescue of NB4-2 is seen in approximately 43% of hemisegments. Expression of slp2hs.PC in wgl-8 or wgl-17 embryos rescues the RP2 lineage in approximately 50% of hemisegments.

Addition of W05014 or Df(3L)H99 does not result in any pronounced improvement of the wgl-8 segment polarity defect; all cells still secrete a uniform lawn of denticles and the cuticle remains smaller than wild type in double mutant embryos. However, the number of denticles is more than doubled in wgl-8 ; Df(3L)H99 embryos compared to wgl-8 single mutants, and the denticles secreted by the double mutants are much smaller than in wgl-8 single mutants. The effect of a reduction in W+ dose on wgl-8 is additive The number of rows of ventral epidermis cells is increased to 12-14 per segment in wgl-8 ; Df(3L)H99 embryos and the cells are much smaller than in wgl-8 single mutants. Most of the cells are cuboidal, they create a pattern of block-like pseudosegments and all cells secrete denticles in the double mutant embryos.

wgl-8,pucA251.1F3 double mutants show a novel loss of dorsal cuticle phenotype. Dorsal closure is never initiated. Lateral epidermal cells elongate along the AP axis and resemble leading edge cells. Massive cell degeneration is seen in the lateral and dorsal epidermis of late embryos.

Wings on wgl-8/+; dally06464/dally06464 animals show a stronger wing notch phenotype than dally06464/dally06464 alone at a higher penetrance (3% to 8%).

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (2)
Notes on Origin
Discoverer

Nusslein-Volhard.

Comments
Comments

Pairwise complementation analysis of wg1, wgl-8, wgH and an allele of spd reveals a complex complementation pattern.

The wg phenotype is suppressed by mutations in nkd and enhanced by mutations in hh.

Strong wg allele.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (6)
Reported As
Symbol Synonym
Name Synonyms
Secondary FlyBase IDs
    References (70)