wgl-8 heterozygosity does not suppress the increased mitotic index in the adult midgut epithelium induced by the adulthood-only expression of UvragHMS01357 under the control of Scer\GAL4esg-NP5130 (and Gal80[ts], for the temporal control of expression).
The segment polarity phenotype and the lack of naked cuticle between denticle belts on the ventral epidermis characteristic for zygotically mutant wgl-8 embryos is not modified by Scer\GAL4arm.PU-driven expression of either sggScer\UAS.T:Ivir\HA1 or sggKK-MI.Scer\UAS.T:Myr-Src64B,T:Ivir\HA1.
The naked cuticle phenotype (loss of denticle belts on the ventral epidermis) observed in embryos expressing sggScer\UAS.T:Myr-Src64B,T:Ivir\HA1 under the control of Scer\GAL4arm.PU is not affected by combination with wgl-8 homozygosity (which on its own leads to loss of naked cuticle and ectopic denticles).
he increase in average cell number in the stalks between egg chambers which is seen in Mmp2Y53N/Df(2R)BSC132 females after being shifted to the restrictive temperature is dominantly suppressed if the females also carry a single copy of wgl-8.
Expression of slp1hs.PC in wgl-8 embryos rescues the RP2/sib lineage in as many as 85% of hemisegments. Rescue of NB4-2 is seen in approximately 43% of hemisegments. Expression of slp2hs.PC in wgl-8 or wgl-17 embryos rescues the RP2 lineage in approximately 50% of hemisegments.
Addition of W05014 or Df(3L)H99 does not result in any pronounced improvement of the wgl-8 segment polarity defect; all cells still secrete a uniform lawn of denticles and the cuticle remains smaller than wild type in double mutant embryos. However, the number of denticles is more than doubled in wgl-8 ; Df(3L)H99 embryos compared to wgl-8 single mutants, and the denticles secreted by the double mutants are much smaller than in wgl-8 single mutants. The effect of a reduction in W+ dose on wgl-8 is additive The number of rows of ventral epidermis cells is increased to 12-14 per segment in wgl-8 ; Df(3L)H99 embryos and the cells are much smaller than in wgl-8 single mutants. Most of the cells are cuboidal, they create a pattern of block-like pseudosegments and all cells secrete denticles in the double mutant embryos.
wgl-8,pucA251.1F3 double mutants show a novel loss of dorsal cuticle phenotype. Dorsal closure is never initiated. Lateral epidermal cells elongate along the AP axis and resemble leading edge cells. Massive cell degeneration is seen in the lateral and dorsal epidermis of late embryos.