FlyBase Allele Report: Dmel\wg[l-8]

wg^l-8 embryos show segment polarity phenotype and no naked cuticle between denticle belts on the ventral epidermis (resulting in a lawn of denticles).

(Mannava and Tolwinski, 2015)

All epidermal cells secrete denticles in the mutant embryo.

(Peradziryi et al., 2011)

wg^l-8 mutant embryos have no second midgut constriction.

(Takemaru et al., 2003)

Embryos completely lack segment polarity and have a lawn of uniform, large, thick denticles covering the ventral epidermis. The embryos are much smaller than wild type. They are usually closed dorsally. There are only 8 rows of ventral epidermal cells per segment in wg^l-8 embryos (wild-type number is, on average, 12 rows per segment). The cells are larger than normal and are cuboidal. Cells in the ventral epidermis enter mitosis 16 in mutant embryos (as occurs in wild type) and mitotic figures are as readily apparent in the ventral epidermis in stage 12 mutant embryos as they are in wild-type embryos.

(Cox et al., 2000)

Patterning defects evident in the denticle belts. Many mutant embryos are dorsally closed but exhibit severe defects in dorsal patterning, with loss of dorsal hairs and the presence of abnormal cuticular structures. A proportion show a mild dorsal-open phenotype. Leading edge cells never elongate along the DV axis, instead they stretch along the AP axis. Certain lateral cells contract like leading edge cells, stretching along the AP axis.

(McEwen et al., 2000)

Posterior cells of each segment are transformed to anterior fates in homozygous embryos and secrete denticles. Most of the denticles of these embryos resemble the large type found in normal row 5. Denticle orientation is often reversed or aligned towards the ventral midline.

(Kennerdell and Carthew, 1998)

Homozygous embryos lack RP2 neurons.

(Loureiro and Peifer, 1998)

Embryos are shorter than wild-type and secrete only denticles with no naked cuticle. wg^l-8 embryos expressing arm^{S10.Scer\UAS.T:Hsap\MYC} (using Scer\GAL4^e22c) produce a cuticle phenotype similar to embryos expressing arm^{S10.Scer\UAS.T:Hsap\MYC} (using Scer\GAL4^e22c) in a wild-type background; nearly all secreted cuticle is naked. wg^l-8 embryos expressing arm^ΔN.Scer\UAS (using Scer\GAL4^e22c) secrete naked cuticle interspersed with denticles on their ventral surface, while the dorsal surface is unchanged from the wg^l-8 mutant phenotype.

(Pai et al., 1997)

Only one central invagination fold of the stomodeal invagination is observed.

(Gonzalez-Gaitan and Jäckle, 1995)

Embryos exhibit a lawn of denticle belts.

(Manoukian et al., 1995)

No reduction in rate of germ band extension, in wg, ptc, en triple mutants.

(Irvine and Wieschaus, 1994)

Similar mutant leg phenotypes, e.g. duplications and bifurcations, are produced by dsh clones, dsh, wg double heterozygotes and wg mutants. There is allele specificity in these interactions.

(Theisen et al., 1994)

Defective in gonad assembly.

(Warrior, 1994)

Mutant embryos have a lawn of row-5-type ventral denticles, with segmental polarity reversals.

(Bejsovec and Wieschaus, 1993)

hh expression in the embryo begins to fade as the germ band reaches full extension, though it does persist in a few midline cells which may be neuroblasts.

(Lee et al., 1992)

Before stage 10 wg embryos have normal hh expression, but it begins to decay at stage 10 and is gone by stage 11.

(Tabata et al., 1992)

Very strong segment polarity phenotype.

(Peifer et al., 1991)

wg^l-8 and wg^l-17 embryos show no localized increases of arm protein in the epidermis at the time the stripes would be apparent in wild type embryos, but increases were seen in domains that do not express wg.

(Riggleman et al., 1990)

The RP2 neuron is the only identifiable cell altered. The restricted phenotype may be due to improper communication between neurons.

(Patel et al., 1989)

Embryos lack head cuticle and there are no or very rudimentary filzkorper. Embryos show an aberrant pattern of cell death and lack of parasegmental and segmental boundaries.

(Perrimon and Mahowald, 1987)

embryonic lethal

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhanced by

Statement

Reference

wg^l-8 has segment polarity phenotype | embryonic stage phenotype, non-enhanceable by sgg^{KK-MI.UAS.Tag:Myr(Src64B),Tag:HA}/Scer\GAL4^arm.PU

(Mannava and Tolwinski, 2015)

wg^l-8 has segment polarity phenotype | embryonic stage phenotype, non-enhanceable by sgg^UAS.Tag:HA/Scer\GAL4^arm.PU

(Mannava and Tolwinski, 2015)

Suppressed by

Statement

Reference

wg^l-8 has increased cell size | recessive phenotype, suppressible by Df(3L)H99

(Cox et al., 2000)

wg^l-8 has decreased cell number | recessive phenotype, suppressible by Df(3L)H99

(Cox et al., 2000)

NOT suppressed by

Statement

Reference

wg^l-8 has segment polarity phenotype | embryonic stage phenotype, non-suppressible by sgg^UAS.Tag:HA/Scer\GAL4^arm.PU

(Mannava and Tolwinski, 2015)

wg^l-8 has segment polarity phenotype | embryonic stage phenotype, non-suppressible by sgg^{KK-MI.UAS.Tag:Myr(Src64B),Tag:HA}/Scer\GAL4^arm.PU

(Mannava and Tolwinski, 2015)

Enhancer of

Statement

Reference

wg[+]/wg^l-8 is an enhancer of visible | recessive phenotype of dally⁰⁶⁴⁶⁴

(Lin and Perrimon, 1999)

NOT Enhancer of

Statement

Reference

wg^l-8 is a non-enhancer of visible phenotype of upd1^GMR.PB

(Bach et al., 2003)

wg[+]/wg^l-8 is a non-enhancer of visible | heat sensitive phenotype of fz^I.hs

(Winter et al., 2001)

Suppressor of

Statement

Reference

wg[+]/wg^l-8 is a suppressor | partially of abnormal size phenotype of Scer\GAL4^pnr-MD237, hep^UAS.cBa

(Zhang et al., 2015)

wg[+]/wg^l-8 is a suppressor | partially of visible phenotype of Scer\GAL4^pnr-MD237, hep^UAS.cBa

(Zhang et al., 2015)

wg[+]/wg^l-8 is a suppressor of abnormal size phenotype of Scer\GAL4^GMR.PF, hep^CA.UAS

(Zhang et al., 2015)

wg[+]/wg^l-8 is a suppressor of visible phenotype of Scer\GAL4^GMR.PF, hep^CA.UAS

(Zhang et al., 2015)

wg[+]/wg^l-8 is a suppressor of increased cell number | female | adult stage | heat sensitive phenotype of Df(2R)BSC132/Mmp2^Y53N

(Wang and Page-McCaw, 2014)

wg[+]/wg^l-8 is a suppressor of abnormal neuroanatomy phenotype of spin¹⁰⁴⁰³

(Yuva-Aydemir et al., 2011)

NOT Suppressor of

Statement

Reference

wg[+]/wg^l-8 is a non-suppressor of increased occurrence of cell division | adult stage phenotype of Scer\GAL4^NP5130, Uvrag^HMS01357

(Nagy et al., 2016)

wg^l-8 is a non-suppressor of visible phenotype of upd1^GMR.PB

(Bach et al., 2003)

wg[+]/wg^l-8 is a non-suppressor of visible | heat sensitive phenotype of fz^I.hs

(Winter et al., 2001)

Phenotype Manifest In

Enhanced by

Statement

Reference

wg^l-8 has embryonic epidermis | dorsal phenotype, enhanceable by puc^A251.1F3

(McEwen et al., 2000)

wg^l-8 has embryonic/first instar larval cuticle | dorsal phenotype, enhanceable by puc^A251.1F3

(McEwen et al., 2000)

NOT Enhanced by

Statement

Reference

wg^l-8 has embryonic/larval cuticle | embryonic stage phenotype, non-enhanceable by sgg^UAS.Tag:HA/Scer\GAL4^arm.PU

(Mannava and Tolwinski, 2015)

wg^l-8 has denticle field | embryonic stage phenotype, non-enhanceable by sgg^UAS.Tag:HA/Scer\GAL4^arm.PU

(Mannava and Tolwinski, 2015)

wg^l-8 has embryonic/larval cuticle | embryonic stage phenotype, non-enhanceable by sgg^{KK-MI.UAS.Tag:Myr(Src64B),Tag:HA}/Scer\GAL4^arm.PU

(Mannava and Tolwinski, 2015)

wg^l-8 has denticle field | embryonic stage phenotype, non-enhanceable by sgg^{KK-MI.UAS.Tag:Myr(Src64B),Tag:HA}/Scer\GAL4^arm.PU

(Mannava and Tolwinski, 2015)

Suppressed by

Statement

Reference

wg^l-8 has RP2sib neuron phenotype, suppressible by slp1^hs.PC

(Bhat et al., 2000)

wg^l-8 has neuroblast NB4-2 phenotype, suppressible by slp1^hs.PC

(Bhat et al., 2000)

wg^l-8 has larval RP2 motor neuron phenotype, suppressible by slp1^hs.PC

(Bhat et al., 2000)

wg^l-8 has larval RP2 motor neuron phenotype, suppressible by slp2^hs.PC

(Bhat et al., 2000)

NOT suppressed by

Statement

Reference

wg^l-8 has embryonic/larval cuticle | embryonic stage phenotype, non-suppressible by sgg^UAS.Tag:HA/Scer\GAL4^arm.PU

(Mannava and Tolwinski, 2015)

wg^l-8 has embryonic/larval cuticle | embryonic stage phenotype, non-suppressible by sgg^{KK-MI.UAS.Tag:Myr(Src64B),Tag:HA}/Scer\GAL4^arm.PU

(Mannava and Tolwinski, 2015)

wg^l-8 has denticle field | embryonic stage phenotype, non-suppressible by sgg^UAS.Tag:HA/Scer\GAL4^arm.PU

(Mannava and Tolwinski, 2015)

wg^l-8 has denticle field | embryonic stage phenotype, non-suppressible by sgg^{KK-MI.UAS.Tag:Myr(Src64B),Tag:HA}/Scer\GAL4^arm.PU

(Mannava and Tolwinski, 2015)

wg^l-8 has embryo | segment polarity expression pattern phenotype, non-suppressible by Df(3L)H99

(Cox et al., 2000)

wg^l-8 has embryo | segment polarity expression pattern phenotype, non-suppressible by hid⁰⁵⁰¹⁴

(Cox et al., 2000)

wg^l-8 has ventral denticle belt phenotype, non-suppressible by puc^A251.1F3

(McEwen et al., 2000)

wg^l-8 has embryonic epidermis | ventral phenotype, non-suppressible by puc^A251.1F3

(McEwen et al., 2000)

Enhancer of

Statement

Reference

wg[+]/wg^l-8 is an enhancer of wing phenotype of dally⁰⁶⁴⁶⁴

(Lin and Perrimon, 1999)

wg[+]/wg^l-8 is an enhancer of wing margin bristle phenotype of dally⁰⁶⁴⁶⁴

(Lin and Perrimon, 1999)

NOT Enhancer of

Statement

Reference

wg^l-8 is a non-enhancer of eye phenotype of upd1^GMR.PB

(Bach et al., 2003)

wg[+]/wg^l-8 is a non-enhancer of wing hair | increased number phenotype of fz^I.hs

(Winter et al., 2001)

Suppressor of

Statement

Reference

wg[+]/wg^l-8 is a suppressor | partially of scutellum phenotype of Scer\GAL4^pnr-MD237, hep^UAS.cBa

(Zhang et al., 2015)

wg[+]/wg^l-8 is a suppressor of eye phenotype of Scer\GAL4^GMR.PF, hep^CA.UAS

(Zhang et al., 2015)

wg[+]/wg^l-8 is a suppressor of stalk follicle cell | increased number | heat sensitive phenotype of Df(2R)BSC132/Mmp2^Y53N

(Wang and Page-McCaw, 2014)

wg[+]/wg^l-8 is a suppressor of stalk follicle cell | increased number | heat sensitive phenotype of Df(2R)BSC132/Mmp2^Y675N

(Wang and Page-McCaw, 2014)

wg[+]/wg^l-8 is a suppressor of eye disc phenotype of spin¹⁰⁴⁰³

(Yuva-Aydemir et al., 2011)

wg[+]/wg^l-8 is a suppressor of glial cell phenotype of spin¹⁰⁴⁰³

(Yuva-Aydemir et al., 2011)

NOT Suppressor of

Statement

Reference

wg[+]/wg^l-8 is a non-suppressor of embryonic/larval neuromuscular junction | larval stage phenotype of DAAM^Ex68

(Migh et al., 2018)

wg[+]/wg^l-8 is a non-suppressor of NMJ bouton | larval stage phenotype of DAAM^Ex68

(Migh et al., 2018)

wg[+]/wg^l-8 is a non-suppressor of larval LL1 motor neuron | larval stage phenotype of DAAM^Ex68

(Migh et al., 2018)

wg[+]/wg^l-8 is a non-suppressor of adult midgut epithelium phenotype of Scer\GAL4^NP5130, Uvrag^HMS01357

(Nagy et al., 2016)

wg^l-8/wg^l-8 is a non-suppressor of embryonic/larval cuticle | embryonic stage phenotype of Scer\GAL4^arm.PU, sgg^{UAS.Tag:Myr(Src64B),Tag:HA}

(Mannava and Tolwinski, 2015)

wg^l-8/wg^l-8 is a non-suppressor of denticle field | embryonic stage phenotype of Scer\GAL4^arm.PU, sgg^{UAS.Tag:Myr(Src64B),Tag:HA}

(Mannava and Tolwinski, 2015)

wg^l-8 is a non-suppressor of arista | increased number phenotype of obk¹

(Atallah et al., 2004)

wg^l-8 is a non-suppressor of eye phenotype of upd1^GMR.PB

(Bach et al., 2003)

wg[+]/wg^l-8 is a non-suppressor of wing hair | increased number phenotype of fz^I.hs

(Winter et al., 2001)

Other

Statement

Reference

ovo^svb-108, wg[+]/wg^l-8 has dorsal fine hair phenotype

(Frankel et al., 2010)

Additional Comments

Genetic Interactions

Statement

Reference

wg^l-8 heterozygosity does not suppress the increased mitotic index in the adult midgut epithelium induced by the adulthood-only expression of Uvrag^HMS01357 under the control of Scer\GAL4^esg-NP5130 (and Gal80[ts], for the temporal control of expression).

(Nagy et al., 2016)

The segment polarity phenotype and the lack of naked cuticle between denticle belts on the ventral epidermis characteristic for zygotically mutant wg^l-8 embryos is not modified by Scer\GAL4^arm.PU-driven expression of either sgg^{Scer\UAS.T:Ivir\HA1} or sgg^{KK-MI.Scer\UAS.T:Myr-Src64B,T:Ivir\HA1}.

The naked cuticle phenotype (loss of denticle belts on the ventral epidermis) observed in embryos expressing sgg^{Scer\UAS.T:Myr-Src64B,T:Ivir\HA1} under the control of Scer\GAL4^arm.PU is not affected by combination with wg^l-8 homozygosity (which on its own leads to loss of naked cuticle and ectopic denticles).

(Mannava and Tolwinski, 2015)

he increase in average cell number in the stalks between egg chambers which is seen in Mmp2^Y53N/Df(2R)BSC132 females after being shifted to the restrictive temperature is dominantly suppressed if the females also carry a single copy of wg^l-8.

(Wang and Page-McCaw, 2014)

ovo^svb-108/Y ; wg^l-8/+ larvae raised at 25[o]C have significantly fewer quaternary trichomes compared to controls.

(Frankel et al., 2010)

Loss of the second midgut constriction in wg^l-8 mutant embryos is suppressed by Cby^dsRNA.cTa. Loss of the first midgut constriction in Cby^dsRNA.cTa injected embryos is partially suppressed by wg^l-8.

(Takemaru et al., 2003)

Expression of slp1^hs.PC in wg^l-8 embryos rescues the RP2/sib lineage in as many as 85% of hemisegments. Rescue of NB4-2 is seen in approximately 43% of hemisegments. Expression of slp2^hs.PC in wg^l-8 or wg^l-17 embryos rescues the RP2 lineage in approximately 50% of hemisegments.

(Bhat et al., 2000)

Addition of W⁰⁵⁰¹⁴ or Df(3L)H99 does not result in any pronounced improvement of the wg^l-8 segment polarity defect; all cells still secrete a uniform lawn of denticles and the cuticle remains smaller than wild type in double mutant embryos. However, the number of denticles is more than doubled in wg^l-8 ; Df(3L)H99 embryos compared to wg^l-8 single mutants, and the denticles secreted by the double mutants are much smaller than in wg^l-8 single mutants. The effect of a reduction in W⁺ dose on wg^l-8 is additive The number of rows of ventral epidermis cells is increased to 12-14 per segment in wg^l-8 ; Df(3L)H99 embryos and the cells are much smaller than in wg^l-8 single mutants. Most of the cells are cuboidal, they create a pattern of block-like pseudosegments and all cells secrete denticles in the double mutant embryos.

(Cox et al., 2000)

wg^l-8,puc^A251.1F3 double mutants show a novel loss of dorsal cuticle phenotype. Dorsal closure is never initiated. Lateral epidermal cells elongate along the AP axis and resemble leading edge cells. Massive cell degeneration is seen in the lateral and dorsal epidermis of late embryos.

(McEwen et al., 2000)

Wings on wg^l-8/+; dally⁰⁶⁴⁶⁴/dally⁰⁶⁴⁶⁴ animals show a stronger wing notch phenotype than dally⁰⁶⁴⁶⁴/dally⁰⁶⁴⁶⁴ alone at a higher penetrance (3% to 8%).

(Lin and Perrimon, 1999)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

5351

wg^l-8/CyO; P{ftz-lacC}1

Kyoto

107019

wg^l-8 cn¹ bw¹ speck¹/CyO

Notes on Origin

Discoverer

Nusslein-Volhard.

Comments

Pairwise complementation analysis of wg¹, wg^l-8, wg^H and an allele of spd reveals a complex complementation pattern.

(Tiong and Nash, 1990)

The wg phenotype is suppressed by mutations in nkd and enhanced by mutations in hh.

(Bejsovec and Wieschaus, 1993)

Strong wg allele.

(Bhat, 1996)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (6)

Reported As

Symbol Synonym

wg^1-8

(Nagy et al., 2016, Cheng et al., 2014, Doumpas et al., 2013, Takemaru et al., 2003, Kennerdell and Carthew, 1998)

wg^1g22

(Winter et al., 2001)

wg^IG22

(Tan et al., 2024, Schaefer et al., 2018, Suresh et al., 2017, Mannava and Tolwinski, 2015, Zhang et al., 2015, Wang and Page-McCaw, 2014, Peradziryi et al., 2011, Frankel et al., 2010, Yan and Lin, 2007, Estrada et al., 2006, Cox and Baylies, 2005, Tolwinski and Wieschaus, 2004, Bach et al., 2003, Tolwinski et al., 2003, Zhou et al., 2001, Alcedo et al., 2000, Bhat et al., 2000, Cox et al., 2000, Halfon et al., 2000, McEwen et al., 2000, Lin and Perrimon, 1999, Carmena et al., 1998, Fuss and Hoch, 1998, Loureiro and Peifer, 1998, Richter et al., 1998, Bhat and Schedl, 1997, Buratovich et al., 1997, Pai et al., 1997, van de Wetering et al., 1997, Bhat, 1996, Goriely et al., 1996, Hoch and Pankratz, 1996, Rulifson et al., 1996, Manoukian et al., 1995, Pankratz and Hoch, 1995, Schwartz et al., 1995, Yoffe et al., 1995, Irvine and Wieschaus, 1994, Peifer et al., 1994, Peifer et al., 1994, Warrior, 1994, Bejsovec and Wieschaus, 1993, Li and Noll, 1993, Li et al., 1993, van den Heuvel et al., 1993, van den Heuvel et al., 1993, Lee et al., 1992, Tabata et al., 1992, Peifer et al., 1991, Riggleman et al., 1990, Patel et al., 1989, Frasch and Levine, 1987, Perrimon and Mahowald, 1987)

wg^IG23

(Gonzalez-Gaitan and Jäckle, 1995)

wg^IG

(Hing et al., 1994, Theisen et al., 1994, Klingler and Gergen, 1993, Tearle and Nusslein-Volhard, 1987)

wg^l-8

(Kanaoka et al., 2023, Kim et al., 2021, Bosch et al., 2020, Bosch and Perrimon, 2019.9.30, Migh et al., 2018, Yuva-Aydemir et al., 2011)

Name Synonyms

Secondary FlyBase IDs

References (74)

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