Southern blotting shows a breakpoint in the +14.6 - +16.3 interval, DNA right of +16.3 is still present demonstrating that the 3' breakpoint is not one end of a deficiency. The relationship of the wgl-16 lesion to the cytology of the Df(2L)wg-CX3 chromosome is not clear; they might be related parts of a complex rearrangement, or might be independent events in the same chromosomal region. The wgl-16 mutation is associated with an insertion of unknown DNA just 3' to the transcription unit (FBrf0046100; FBrf0066940).
Insertion of DNA in the 3' control region.
17kb insertion into the 3' regulatory region.
Deletion that brings other sequences close to the transcription terminus, thereby reducing the production of mRNA and protein.
break between +10.8 and 15.0 kb
Flies carrying Stat92E85C9 Minute clones in a wgl-16/+ background show a significantly increased rate of eclosion compared to those with a wild-type background. Further, eclosed wgl-16/+; Stat92E85C9 Minute flies show less severe phenotypes compared to eclosed Stat92E85C9 Minute flies.
Many of the phenotypes caused by wgl-16/wgl-17 can be rescued by expression of Wnt4Scer\UAS.cGa under the control of Scer\GAL4ptc-559.1; wing rescue is seen at high frequency, halteres appear well developed, leg morphology is often rescued, but partial rescue of the antenna is only occasionally seen.
Expression of wgScer\UAS.cLa under the control of Scer\GAL4cad-em459 in wgl-16/wgSp-1 females sometimes results in recovery of the vaginal plates and tergite eight, but not the vulva. The analia are much reduced in some cases and some females have no genital and anal structures. Expression of wgScer\UAS.cLa under the control of Scer\GAL4cad-em459 in wgl-16/wgSp-1 males results in recovery of genital structures such as the claspers and penis apparatus, although the penis apparatus is sometimes still reduced. Analia are almost normal or reduced.