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General Information
Symbol
Dmel\eya1
Species
D. melanogaster
Name
FlyBase ID
FBal0028035
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
eya, clieya
Allele class
Mutagen
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
deletion
Comment:

An 1826 bp deletion that extends from 581 bp upstream of the start site of the eya2 deletion into the 5'UTR of the eya-RB transcript.

Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

1826bp deletion extending from 581bp upstream of the start site of the deletion present in the eya2 mutant and extends 344bp into the 5'UTR of the eya-RB transcript. An additional 11bp that do not correspond to the published genome sequence are also present.

1.5kb deletion upstream of the transcription start site of the type I eya transcript.

Small deletion outside the coding region.

Caused by aberration
Expression Data
Reporter Expression
Additional Information
Statement
Reference

A 3.5kb eya transcript is present in adult eya1 heads at reduced levels relative to wild type.

 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Adult eya1 homozygous mutants are eyeless, eya1 homozygous flies carrying either the eyaRB.enhE or the eyaRB.enh1 transgene display small eyes (partial rescue).

eya1 mutants exhibit normal light-dependent temperature preference. Similarly to wild-type, they prefer higher temperature in the light than in the dark.

eya1 flies can synchronise to dim-light/dark cycles.

Once synchronised under standard light/dark conditions, eya1 flies do not respond at all to nocturnal moonlight, and do not shift their activity patterns in response to light/moonlight cycles.

Under LD conditions eya1 flies exhibit the typical bimodal activity pattern consisting of M and E activity bouts, as in wild-type. After transfer into LL, eya1 flies become immediately arrhythmic, as in wild-type.

eya1/eyaE1, eya1/eyaE8, eya1/eyaE12 or eya1/eyacli-IID flies are eyeless or have eyes reduced to less than 1/4 normal size. eya1/eyaE4 flies have eyes that are less than 1/4 normal size. eya1/eyaE7 flies have eyes 1/4 to less than 1/2 normal size. eya1/eyaE11 flies have eyes 3/4 to less than normal size. eya1/eyaIR1 or eya1/eyaIR3 flies are eyeless or have eyes reduced to less than 1/2 normal size. eya1/eyaE(P)10 flies are eyeless.

Homozygotes completely lack eyes.

Homozygotes are eyeless. eya1/eya3 flies have eyes that are between 3/4 and less than normal size, and eya1/eya3 females are fertile. Produces eyeless flies in combination with eya2, eyaE1, eyaE5, eyaE10, eyaD1, eyaD2, eyaD3, eyaD4, eyaD5, eyaD6, eyaD7 or Df(2L)eya10. Produces flies with reduced eyes in combination with eya4, eyaE3, eyaE4, eyaE6, eyaE7, eyaE9 or eyaE11. Produces flies which are either eyeless or have reduced eyes in combination with eyaE2, eyaE8, eyaE12, eyacli-IID or eyaZ283.

Photoreceptors do not develop within homozygous clones in the eye disc.

R cell differentiation is blocked.

eya1/eyaE3 flies are viable, and have eyes which range from 1/2 to less than normal size. eya1/eya4 flies have eyes which range from 1/2 to less than normal size.

Flies lack retinal innervation of the lamina, and lamina precursor cells fail to divide.

Displays locomotor activity rhythm with an approximately 24h period.

Lacks compound eyes because the eye disc fails to differentiate. BrdU incorporation experiments show that cell division is not abnormal, but cell death is dramatically increased.

Lacks compound eyes, retains simple eyes (ocelli). Phospholipid distribution within the fly head is essentially the same as in wild type.

Geotaxis score no different from Canton S in a Hirsch-type geotaxis maze.

Transheterozygotes with eya4 have reduced eyes with fewer facets than normal and the facets are disorganised. The posterior vertical bristles of the head are truncated with frayed ends.

viable Eye facets completely absent in strong alleles; other aspects of head development appear normal; antennae and ocelli, which also arise from eye-antenna disc, present. Eye portion of disc markedly reduced; no morphogenetic furrow or putative photoreceptor-cell clusters seen; little or no staining with monoclonal antibodies that normally stain presumptive photoreceptor cells. most of them are homozygous lethal. Some alleles are temperature-sensitive.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
Statement
Reference
NOT suppressed by
Statement
Reference

eya1, eyaRB.enhE has visible | adult stage phenotype, non-suppressible by eyaRB.PSE

eya1, eyaRB.enhE has visible | adult stage phenotype, non-suppressible by eyaRB.enh2

eya1, eyaRB.enhE has visible | adult stage phenotype, non-suppressible by eyaRB.enh3

eya1, eyaRB.enhE has visible | adult stage phenotype, non-suppressible by eyaRB.enh4

Enhancer of
Statement
Reference
Suppressor of
Statement
Reference

eya1 is a suppressor of viable phenotype of DNaseIIlo

Phenotype Manifest In
Suppressed by
Statement
Reference

eya1, eyaRB.enhE has eye phenotype, suppressible by eyaRB.enh1

eya1, eyaRB.enh1 has eye | adult stage phenotype, suppressible by eyaRB.enhE

NOT suppressed by
Statement
Reference

eya1, eyaRB.enhE has eye phenotype, non-suppressible by eyaRB.enh4

eya1, eyaRB.enhE has eye phenotype, non-suppressible by eyaRB.PSE

eya1, eyaRB.enhE has eye phenotype, non-suppressible by eyaRB.enh2

eya1, eyaRB.enhE has eye phenotype, non-suppressible by eyaRB.enh3

Enhancer of
Statement
Reference

eya1 is an enhancer of pacemaker neuron phenotype of cryb

Other
Statement
Reference
Additional Comments
Genetic Interactions
Statement
Reference

The small eye phenotype characteristic for eya1 homozygotes carrying the eyaRB.enhE transgene, i.e. partial rescue of the eyeless phenotype of the eya1 mutants, is improved further by combination with eyaRB.enh1 (which on its own also partially rescues the no-eye phenotype of eya1 mutants) but not by combination with any of the following: eyaRB.enh2, eyaRB.enh3, eyaRB.enh4 or eyaRB.PSE (none of which on its own can rescue the eyeless phenotype of eya1 adults).

Flies carrying homozygous eya1 and DNaseIIlo mutations die at the pupal stage.

The main difference between cryb and eya1;cryb double mutants is the absence of the compound eyes in the eya1;cryb flies.

Under LD conditions eya1;cryb double mutant flies exhibit the typical bimodal activity pattern consisting of M and E activity bouts, as in wild-type. After transfer into LL conditions, eya1;cryb flies remain rhythmic and free-run with a period close to 24 hours (whereas wild-type flies become immediately arrhythmic).

eya1;cryb double mutants do not exhibit internal desynchronisation under LL conditions, and free-run with a wild-type-like period.

When eyScer\UAS.cHa is driven by Scer\GAL4dpp.blk1 in a so1 or eya1 background, no eye structures appear but legs are reduced/deformed.

The lack of photoreceptor phenotype of homozygous eye discs is not rescued by tkvQ253D.Scer\UAS.cNb, sggS9A.Scer\UAS, hhScer\UAS.cAa or Ras85DV12.Scer\UAS expressed under the control of Scer\GAL4ey.PH.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Fails to complement
Partially rescued by

eya1 is partially rescued by eyaRB.enh1

eya1 is partially rescued by eyaRB.enh1

eya1 is partially rescued by eyaRB.enhE

eya1 is partially rescued by eyaRB.enhE

eya1 is partially rescued by eyaRB.enh1+E

eya1 is partially rescued by eyaRB.enh1+E

eya1 is partially rescued by eyaRB.enh1+s+2

eya1 is partially rescued by eyaRB.enh1+s+2

Not rescued by
Comments

The no-eye phenotype of adult eya1 homozygous mutants can be fully rescued by combination with eyaRB.enh1+E+2 and partially restored by combination with any of the following: eyaRB.enh1, eyaRB.enhE, eyaRB.enh1+E or eyaRB.enh1+s+2 but cannot be rescued by any of these transgenes: eyaRB.enh2, eyaRB.enh3, eyaRB.enh4 or eyaRB.PSE.

More than 40 alleles obtained by X-ray and EMS mutagenesis.

Images (1)
Stocks (4)
Notes on Origin
Discoverer
Comments
Comments

Has been used by several groups in subtractive hybridization protocols for the isolation of eye-specific cDNA's.

eya1 and eya2 form an intragenic complementation group that interacts with eya3 and eya4.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (6)
References (48)