FlyBase Allele Report: Dmel\eya[1]

General Information

Symbol

Dmel\eya¹

Species

D. melanogaster

Name

FlyBase ID

FBal0028035

Feature type

allele

Associated gene

Dmel\eya

Associated Insertion(s)

Carried in Construct

Also Known As

eya, cli^eya

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

spontaneous

Nature of the Allele

Allele class

Mutagen

spontaneous

(Bonini et al., 1998, Leiserson et al., 1994)

Progenitor genotype

Caused by aberration

In(2L)eya

Cytology

Description

1826bp deletion extending from 581bp upstream of the start site of the deletion present in the eya² mutant and extends 344bp into the 5'UTR of the eya-RB transcript. An additional 11bp that do not correspond to the published genome sequence are also present.

(Weasner et al., 2016)

1.5kb deletion upstream of the transcription start site of the type I eya transcript.

(Zimmerman et al., 2000)

Small deletion outside the coding region.

(Leiserson et al., 1994)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

deletion

2L:6,546,628..6,548,453 [-]

Comment:

An 1826 bp deletion that extends from 581 bp upstream of the start site of the eya² deletion into the 5'UTR of the eya-RB transcript.

(Weasner et al., 2016)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

A 3.5kb eya transcript is present in adult eya¹ heads at reduced levels relative to wild type.

(Bonini et al., 1993)

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal size | adult stage (with eya³)

(Tian et al., 2019)

abnormal size | adult stage (with eya⁴)

(Tian et al., 2019)

abnormal size | adult stage (with eya^cli-IID)

(Tian et al., 2019)

abnormal size | adult stage (with eya^cs)

(Tian et al., 2019)

abnormal size | adult stage (with eya^E1)

(Tian et al., 2019)

abnormal size | adult stage (with eya^E4)

(Tian et al., 2019)

decreased size | adult stage

(Nair et al., 2020)

female fertile

(Bonini et al., 1998)

increased cell death | third instar larval stage

(Bonini et al., 1993)

photoperiod response variant

(Bachleitner et al., 2007)

viable

(Tian et al., 2019, Zimmerman et al., 2000, Bonini et al., 1998, Leiserson et al., 1998)

visible (with eya^cli-IID)

(Bui et al., 2000)

visible (with eya^E1)

(Bui et al., 2000)

visible (with eya^E4)

(Bui et al., 2000)

visible (with eya^E7)

(Bui et al., 2000)

visible (with eya^E8)

(Bui et al., 2000)

visible (with eya^E11)

(Bui et al., 2000)

visible (with eya^E12)

(Bui et al., 2000)

visible (with eya^E(P)10)

(Bui et al., 2000)

visible (with eya^IR1)

(Bui et al., 2000)

visible (with eya^IR3)

(Bui et al., 2000)

visible | adult stage

(Weasner et al., 2016)

visible | adult stage, with eya^RB.enh1

(Weasner et al., 2016)

visible | adult stage, with eya^RB.enhE

(Weasner et al., 2016)

visible | adult stage (with eya³)

(Tian et al., 2019)

visible | adult stage (with eya⁴)

(Tian et al., 2019)

visible | adult stage (with eya^cli-IID)

(Tian et al., 2019)

visible | adult stage (with eya^cs)

(Tian et al., 2019)

visible | adult stage (with eya^E1)

(Tian et al., 2019)

visible | adult stage (with eya^E4)

(Tian et al., 2019)

visible | recessive

(Zimmerman et al., 2000, Bonini et al., 1998, Leiserson et al., 1998, Bonini et al., 1993)

Phenotype Manifest In

adult optic lobe

(Nair et al., 2020)

eye

(Weasner et al., 2016, Head et al., 2015, Zimmerman et al., 2000, Bonini et al., 1998, Leiserson et al., 1998, Frisch et al., 1994, Bonini et al., 1993)

eye, with eya^RB.enh1

(Weasner et al., 2016)

eye, with eya^RB.enhE

(Weasner et al., 2016)

eye (with eya³)

(Tian et al., 2019)

eye (with eya⁴)

(Tian et al., 2019)

eye (with eya^cli-IID)

(Tian et al., 2019, Bui et al., 2000)

eye (with eya^cs)

(Tian et al., 2019)

eye (with eya^E1)

(Tian et al., 2019, Bui et al., 2000, Bui et al., 2000)

eye (with eya^E4)

(Tian et al., 2019, Bui et al., 2000)

eye (with eya^E7)

(Bui et al., 2000)

eye (with eya^E8)

(Bui et al., 2000)

eye (with eya^E11)

(Bui et al., 2000)

eye (with eya^E12)

(Bui et al., 2000)

eye (with eya^E(P)10)

(Bui et al., 2000)

eye (with eya^IR1)

(Bui et al., 2000)

eye (with eya^IR3)

(Bui et al., 2000)

eye disc | somatic clone

(Hazelett et al., 1998)

eye disc | third instar larval stage

(Bonini et al., 1993)

lamina

(Huang and Kunes, 1996)

lamina forming neuroblast

(Huang and Kunes, 1996)

photoreceptor cell & axon

(Huang and Kunes, 1996)

photoreceptor neuron

(Huang et al., 1998)

photoreceptor neuron | somatic clone

(Hazelett et al., 1998)

Detailed Description

Statement

Reference

Some of eya¹/eya^cs adults are eyeless.

eya¹/eya³ and eya¹/eya⁴ adults do not exhibit any differences in the number of ommatidia in the eye while adults containing either genotype exhibit significantly higher number of ommatidia in the eye than eya¹/eya^E1, eya¹/eya^E4 and eya¹/eya^cli-IID adults.

Some eya^cs/eya¹ adults exhibit less severe eye phenotypes than eya^cs/eya² adults, and they exhibit more severe eye phenotypes than eya^cs/In(2LR)ETD2.2, eya^cs/eya^cs and eya^cs/eya^E(P)10 adults.

(Tian et al., 2019)

Adult eya¹ homozygous mutants are eyeless, eya¹ homozygous flies carrying either the eya^RB.enhE or the eya^RB.enh1 transgene display small eyes (partial rescue).

(Weasner et al., 2016)

eya¹ mutants exhibit normal light-dependent temperature preference. Similarly to wild-type, they prefer higher temperature in the light than in the dark.

(Head et al., 2015)

eya¹ flies can synchronise to dim-light/dark cycles.

Once synchronised under standard light/dark conditions, eya¹ flies do not respond at all to nocturnal moonlight, and do not shift their activity patterns in response to light/moonlight cycles.

(Bachleitner et al., 2007)

Under LD conditions eya¹ flies exhibit the typical bimodal activity pattern consisting of M and E activity bouts, as in wild-type. After transfer into LL, eya¹ flies become immediately arrhythmic, as in wild-type.

(Rieger et al., 2006)

eya¹/eya^E1, eya¹/eya^E8, eya¹/eya^E12 or eya¹/eya^cli-IID flies are eyeless or have eyes reduced to less than 1/4 normal size. eya¹/eya^E4 flies have eyes that are less than 1/4 normal size. eya¹/eya^E7 flies have eyes 1/4 to less than 1/2 normal size. eya¹/eya^E11 flies have eyes 3/4 to less than normal size. eya¹/eya^IR1 or eya¹/eya^IR3 flies are eyeless or have eyes reduced to less than 1/2 normal size. eya¹/eya^E(P)10 flies are eyeless.

(Bui et al., 2000)

Homozygotes completely lack eyes.

(Zimmerman et al., 2000)

Homozygotes are eyeless. eya¹/eya³ flies have eyes that are between 3/4 and less than normal size, and eya¹/eya³ females are fertile. Produces eyeless flies in combination with eya², eya^E1, eya^E5, eya^E10, eya^D1, eya^D2, eya^D3, eya^D4, eya^D5, eya^D6, eya^D7 or Df(2L)eya¹⁰. Produces flies with reduced eyes in combination with eya⁴, eya^E3, eya^E4, eya^E6, eya^E7, eya^E9 or eya^E11. Produces flies which are either eyeless or have reduced eyes in combination with eya^E2, eya^E8, eya^E12, eya^cli-IID or eya^Z283.

(Bonini et al., 1998)

Photoreceptors do not develop within homozygous clones in the eye disc.

(Hazelett et al., 1998)

R cell differentiation is blocked.

(Huang et al., 1998)

eya¹/eya^E3 flies are viable, and have eyes which range from 1/2 to less than normal size. eya¹/eya⁴ flies have eyes which range from 1/2 to less than normal size.

(Leiserson et al., 1998)

Flies lack retinal innervation of the lamina, and lamina precursor cells fail to divide.

(Huang and Kunes, 1996)

Displays locomotor activity rhythm with an approximately 24h period.

(Vosshall and Young, 1995)

Lacks compound eyes because the eye disc fails to differentiate. BrdU incorporation experiments show that cell division is not abnormal, but cell death is dramatically increased.

(Bonini et al., 1993)

Lacks compound eyes, retains simple eyes (ocelli). Phospholipid distribution within the fly head is essentially the same as in wild type.

(Stark et al., 1993)

Geotaxis score no different from Canton S in a Hirsch-type geotaxis maze.

(McMillan and McGuire, 1992)

Transheterozygotes with eya⁴ have reduced eyes with fewer facets than normal and the facets are disorganised. The posterior vertical bristles of the head are truncated with frayed ends.

(Eissenberg and Ryerse, 1991)

viable Eye facets completely absent in strong alleles; other aspects of head development appear normal; antennae and ocelli, which also arise from eye-antenna disc, present. Eye portion of disc markedly reduced; no morphogenetic furrow or putative photoreceptor-cell clusters seen; little or no staining with monoclonal antibodies that normally stain presumptive photoreceptor cells. most of them are homozygous lethal. Some alleles are temperature-sensitive.

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

eya^cs/eya¹ has visible | adult stage phenotype, enhanceable by Pc¹/Pc[+]

(Tian et al., 2019)

Suppressed by

Statement

Reference

eya^cs/eya¹ has visible | adult stage phenotype, suppressible | partially by E(z)[+]/E(z)⁶⁰

(Tian et al., 2019)

eya^cs/eya¹ has abnormal size | adult stage phenotype, suppressible | partially by E(z)[+]/E(z)⁶⁰

(Tian et al., 2019)

eya^cs/eya¹ has visible | adult stage phenotype, suppressible | partially by Pc[+]/Pc⁴

(Tian et al., 2019)

eya^cs/eya¹ has abnormal size | adult stage phenotype, suppressible | partially by Pc[+]/Pc⁴

(Tian et al., 2019)

eya¹, eya^RB.enhE has visible | adult stage phenotype, suppressible by eya^RB.enh1

(Weasner et al., 2016)

eya¹, eya^RB.enh1 has visible | adult stage phenotype, suppressible by eya^RB.enhE

(Weasner et al., 2016)

NOT suppressed by

Statement

Reference

eya¹, eya^RB.enhE has visible | adult stage phenotype, non-suppressible by eya^RB.enh2

(Weasner et al., 2016)

eya¹, eya^RB.enhE has visible | adult stage phenotype, non-suppressible by eya^RB.enh3

(Weasner et al., 2016)

eya¹, eya^RB.enhE has visible | adult stage phenotype, non-suppressible by eya^RB.enh4

(Weasner et al., 2016)

eya¹, eya^RB.enhE has visible | adult stage phenotype, non-suppressible by eya^RB.PSE

(Weasner et al., 2016)

Enhancer of

Statement

Reference

eya¹ is an enhancer of abnormal circadian rhythm phenotype of cry^b

(Rieger et al., 2006)

Suppressor of

Statement

Reference

eya¹ is a suppressor of viable phenotype of DNaseII^lo

(Liu et al., 2012)

Phenotype Manifest In

Enhanced by

Statement

Reference

eya^cs/eya¹ has eye phenotype, enhanceable by Pc¹/Pc[+]

(Tian et al., 2019)

Suppressed by

Statement

Reference

eya^cs/eya¹ has eye phenotype, suppressible | partially by E(z)[+]/E(z)⁶⁰

(Tian et al., 2019)

eya^cs/eya¹ has eye phenotype, suppressible | partially by Pc[+]/Pc⁴

(Tian et al., 2019)

eya¹, eya^RB.enhE has eye phenotype, suppressible by eya^RB.enh1

(Weasner et al., 2016)

eya¹, eya^RB.enh1 has eye | adult stage phenotype, suppressible by eya^RB.enhE

(Weasner et al., 2016)

NOT suppressed by

Statement

Reference

eya¹, eya^RB.enhE has eye phenotype, non-suppressible by eya^RB.enh2

(Weasner et al., 2016)

eya¹, eya^RB.enhE has eye phenotype, non-suppressible by eya^RB.enh3

(Weasner et al., 2016)

eya¹, eya^RB.enhE has eye phenotype, non-suppressible by eya^RB.enh4

(Weasner et al., 2016)

eya¹, eya^RB.enhE has eye phenotype, non-suppressible by eya^RB.PSE

(Weasner et al., 2016)

Enhancer of

Statement

Reference

eya¹ is an enhancer of pacemaker neuron phenotype of cry^b

(Rieger et al., 2006)

Other

Statement

Reference

cry^b, eya¹ has eye phenotype

(Rieger et al., 2006)

Scer\GAL4^dpp.blk1, ey^UAS.cHa, eya¹ has leg phenotype

(Halder et al., 1998)

Additional Comments

Genetic Interactions

Statement

Reference

The small eye phenotype characteristic for eya¹ homozygotes carrying the eya^RB.enhE transgene, i.e. partial rescue of the eyeless phenotype of the eya¹ mutants, is improved further by combination with eya^RB.enh1 (which on its own also partially rescues the no-eye phenotype of eya¹ mutants) but not by combination with any of the following: eya^RB.enh2, eya^RB.enh3, eya^RB.enh4 or eya^RB.PSE (none of which on its own can rescue the eyeless phenotype of eya¹ adults).

(Weasner et al., 2016)

Flies carrying homozygous eya¹ and DNaseII^lo mutations die at the pupal stage.

(Liu et al., 2012)

The main difference between cry^b and eya¹;cry^b double mutants is the absence of the compound eyes in the eya¹;cry^b flies.

Under LD conditions eya¹;cry^b double mutant flies exhibit the typical bimodal activity pattern consisting of M and E activity bouts, as in wild-type. After transfer into LL conditions, eya¹;cry^b flies remain rhythmic and free-run with a period close to 24 hours (whereas wild-type flies become immediately arrhythmic).

eya¹;cry^b double mutants do not exhibit internal desynchronisation under LL conditions, and free-run with a wild-type-like period.

(Rieger et al., 2006)

When ey^Scer\UAS.cHa is driven by Scer\GAL4^dpp.blk1 in a so¹ or eya¹ background, no eye structures appear but legs are reduced/deformed.

(Halder et al., 1998)

The lack of photoreceptor phenotype of homozygous eye discs is not rescued by tkv^{Q253D.Scer\UAS.cNb}, sgg^S9A.Scer\UAS, hh^Scer\UAS.cAa or Ras85D^V12.Scer\UAS expressed under the control of Scer\GAL4^ey.PH.

(Hazelett et al., 1998)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Partially complements

eya^X9

(Bonini et al., 1993)

eya^X8

(Bonini et al., 1993)

eya^X3

(Bonini et al., 1993)

eya^X1

(Bonini et al., 1993)

eya^X16

(Bonini et al., 1993)

eya^X15

(Bonini et al., 1993)

eya^X11

(Bonini et al., 1993)

eya^X10

(Bonini et al., 1993)

eya^P7

(Bonini et al., 1993)

eya^P6

(Bonini et al., 1993)

eya^P5

(Bonini et al., 1993)

eya^P4

(Bonini et al., 1993)

eya^P3

(Bonini et al., 1993)

eya^P2

(Bonini et al., 1993)

eya^P1

(Bonini et al., 1993)

eya^E9

(Bonini et al., 1993)

eya^E8

(Bonini et al., 1993)

eya^E7

(Bonini et al., 1993)

eya^E6

(Bonini et al., 1993)

eya^E5

(Bonini et al., 1993)

eya^E4

(Bonini et al., 1993)

eya^E3

(Bonini et al., 1993)

eya^E2

(Bonini et al., 1993)

eya^E1

(Bonini et al., 1993)

eya^E12

(Bonini et al., 1993)

eya^E11

(Bonini et al., 1993)

eya^E10

(Bonini et al., 1993)

eya^X2

(Bonini et al., 1993)

Fails to complement

eya^D1

(Bonini et al., 1998)

Rescued by

eya¹ is rescued by eya^RB.enh1+E+2

(Weasner et al., 2016)

eya¹ is rescued by Scer\GAL4^unspecified/eya^UAS.cBa

(Zimmerman et al., 2000)

eya¹ is rescued by Scer\GAL4^unspecified/eya^UAS.cBb

(Zimmerman et al., 2000)

Partially rescued by

eya¹ is partially rescued by eya^RB.enh1

(Weasner et al., 2016)

eya¹ is partially rescued by eya^RB.enhE

(Weasner et al., 2016)

eya¹ is partially rescued by eya^RB.enh1+E

(Weasner et al., 2016)

eya¹ is partially rescued by eya^RB.enh1+s+2

(Weasner et al., 2016)

Not rescued by

eya¹ is not rescued by eya^RB.enh2

(Weasner et al., 2016)

eya¹ is not rescued by eya^RB.enh3

(Weasner et al., 2016)

eya¹ is not rescued by eya^RB.enh4

(Weasner et al., 2016)

eya¹ is not rescued by eya^RB.PSE

(Weasner et al., 2016)

Comments

The no-eye phenotype of adult eya¹ homozygous mutants can be fully rescued by combination with eya^RB.enh1+E+2 and partially restored by combination with any of the following: eya^RB.enh1, eya^RB.enhE, eya^RB.enh1+E or eya^RB.enh1+s+2 but cannot be rescued by any of these transgenes: eya^RB.enh2, eya^RB.enh3, eya^RB.enh4 or eya^RB.PSE.

(Weasner et al., 2016)

More than 40 alleles obtained by X-ray and EMS mutagenesis.

Images (1)

Holtzman, S. and Kaufman, T. (2013)

Mutant

Wild-type

Stocks (4)

Bloomington

3631

In(2L)eya, eya¹

6336

w^*; In(2L)eya, eya¹ P{neoFRT}40A/CyO

Kyoto

101489

eya¹

107140

In(2L)eya, eya¹

Notes on Origin

Discoverer

Comments

Has been used by several groups in subtractive hybridization protocols for the isolation of eye-specific cDNA's.

eya¹ and eya² form an intragenic complementation group that interacts with eya³ and eya⁴.

(Leiserson et al., 1994)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (6)

Reported As

Symbol Synonym

cli^eya-1

(McNabb and Truman, 2008)

cli^eya

(Schlichting et al., 2019, Bachleitner et al., 2007, Rieger et al., 2006)

eya

(Treisman and Heberlein, 1998, Huang and Kunes, 1996, Pearn et al., 1996, Shah and Hyde, 1995, Zhu et al., 1993, Buckles et al., 1992, Cassill et al., 1991, Eissenberg and Ryerse, 1991, Schneuwly et al., 1991, Lee et al., 1990)

eya1

(Rebay et al., 2000)

eya¹

(Nair et al., 2020, Tian et al., 2019, Weasner et al., 2016, Head et al., 2015, Rosenbaum et al., 2014, Liu et al., 2012, Rosenbaum et al., 2011, Mecklenburg et al., 2010, McNabb and Truman, 2008, Bachleitner et al., 2007, Chotard et al., 2005)

Name Synonyms

eyes absent

Secondary FlyBase IDs

References (50)

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