Allele Dmel\ds^33k

General Information
Symbol	Dmel\ds^33k	Species	D. melanogaster
Name		FlyBase ID	FBal0028155
Feature type	allele	Created / Updated	2006-08-22/2006-08-22
Associated gene	Dmel\ds

Allele class	loss of function
Mutagen	spontaneous, X ray
Nature of the Allele
Allele class	loss of function (Clark et al., 1995)
Mutagen	spontaneous (Clark et al., 1995) X ray
Mapped Features and Mutations
Type Symbol & Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Break at 21D falls within the large intron of ds. Predicted product will be a secreted and truncated protein. (Clark et al., 1995)
Assay mode
Caused by aberration	In(2LR)bw^V1 (Clark et al., 1995, Adler et al., 1998, Araujo et al., 2003, Strutt and Strutt, 2002)
Cytology
Phenotypic Data
Phenotypic Class
	visible (with ds^38k) (Rodriguez, 2004) visible \| recessive (Adler et al., 1998) tissue polarity (with ds^UAO71) (Strutt and Strutt, 2002)
Phenotype Manifest In
	wing hair (with ds^UAO71) (Strutt and Strutt, 2002) wing (with ds^38k) (Rodriguez, 2004) tegula \| ectopic (with ds^38k) (Rodriguez, 2004) humeral sclerite \| ectopic (with ds^38k) (Rodriguez, 2004) wing hinge (with ds^38k) (Rodriguez, 2004) costal vein \| ectopic (with ds^38k) (Rodriguez, 2004) wing margin \| anterior \| ectopic (with ds^38k) (Rodriguez, 2004) wing \| posterior (with ds^38k) (Rodriguez, 2004) wing hinge \| posterior (with ds^38k) (Rodriguez, 2004) scutum \| ectopic (with ds^38k) (Rodriguez, 2004) scutellum \| ectopic (with ds^38k) (Rodriguez, 2004) leg (Clark et al., 1995) wing (Clark et al., 1995, Adler et al., 1998) thorax (Clark et al., 1995) eye (Clark et al., 1995) anterior crossvein (Clark et al., 1995) glial cell & larval optic lobe (Dearborn and Kunes, 2004) axon & larval optic lobe (Dearborn and Kunes, 2004) wing hair (Adler et al., 1998) wing \| proximal (Adler et al., 1998)
Detailed Description
	Statement Reference ds^33k/+ has phenotype like ds^W/+; scutellar bristles wide apart; wings shorter and wider than normal; crossveins close together. ds^33k/ds^W has extreme phenotype like ds^d. RK3A. ds^33k has not been separated from the recessive lethal factor of In(2LR)bw^V1 and, therefore, has not been observed in homozygous condition. In addition to 100% penetrant defects in leg, wing and thorax, rough patches in the eye appear at low frequency. Anterior crossvein is displaced posteriorly. Combinations of ds^33k and ds⁵⁵ with each other or with Df(2L)S2 or Df(2L)ast1 eclose with very low frequency and show very strong deformations of thorax, legs and wings. Imaginal discs of ds^M213/ds^M213 or ds^M213/ds^33k are within the size range of normal discs, showing no tendency to overgrowth. (Clark et al., 1995) ds^33k/ds^UAO71 flies have reduced viability, with only approximately half the expected number of adults emerging. Most of the adults that emerge quickly get stuck in the food and die. These flies also have a severely distorted body shape. ds^33k/ds^UAO71 flies have a wing tissue polarity phenotype similar to that of ds^UAO71 homozygotes and hemizygotes. There is a region at the anterior margin with a swirl of partially reversed polarity hairs. In the anterior/central proximal part of the wing blade there are regions of completely reversed polarity (hairs pointing proximally). These regions are separated from regions of relatively normal polarity by a region of swirling. The polarity disruptions are more severe on the ventral surface of the wing than the dorsal surface. Prehairs initiate in the vicinity of the distal vertex of the wing cells (as in wild-type) in wing regions of ds^33k/ds^UAO71 flies that do not show substantial polarity alterations. In most regions with abnormal polarity, prehairs initiate at an alternative location along the cell periphery. In the regions with reversed polarity, prehairs initiate at the proximal-most vertex, while in regions where hair polarity is close to random and in the regions at the centre of swirls, prehairs initiate in the central regions of the cells. Approximately half the wings of ds^33k/ds¹ flies have a very weak tissue polarity phenotype, consisting of a couple of multiple hair cells and/or a small region of polarity disruption in the proximal part of the wing. (Adler et al., 1998) The wings of ds^33k/ds^UAO71 animals have a trichome swirling pattern that is distinct from that due to fz¹⁵/fz²⁵. (Strutt and Strutt, 2002) Scaffold axons project aberrantly in the larval optic lobes if ds^33k mutants. Glia line these aberrant pathways and accumulate in abnormal destinations such as the lobula cortex. (Dearborn and Kunes, 2004) Occasionally in ds^38k/ds^33k escapers (3 out of 72 heminota scored), the wing is replaced by a winglet and, posterior to it, a lateral protuberance that looks like an scutum and scutellum. The winglet is composed of proximal anterior structures arranged in a mirror-image duplication. The smallest examples of these winglets consist of a mirror image duplication of anterior hinge structures - the tegula and humeral sclerite. Larger examples also have a rudimentary wing blade consisting of a mirror image duplication of anterior structures - the costa and anterior wing margin. (Rodriguez, 2004)
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
Statement Reference ds^33k has visible \| recessive phenotype, suppressible by fz^I.hs (Adler et al., 1998)
NOT Enhancer of
Statement Reference ds^33k/ds[+] is a non-enhancer of visible \| dominant phenotype of sog^EP7 (Araujo et al., 2003) ds^33k/ds[+] is a non-enhancer of visible \| dominant phenotype of sog^EP11 (Araujo et al., 2003)
NOT Suppressor of
Statement Reference ds^33k/ds[+] is a non-suppressor of visible \| dominant phenotype of sog^EP7 (Araujo et al., 2003) ds^33k/ds[+] is a non-suppressor of visible \| dominant phenotype of sog^EP11 (Araujo et al., 2003)
Phenotype Manifest In
Suppressed by
Statement Reference ds^33k has wing phenotype, suppressible by fz^I.hs (Adler et al., 1998) ds^33k has wing hair phenotype, suppressible by fz^I.hs (Adler et al., 1998)
Enhancer of
Statement Reference ds^33k/ds¹ is an enhancer of phenotype of fz^unspecified (Adler et al., 1998) ds^33k is an enhancer of phenotype of fz^unspecified (Adler et al., 1998) ds^33k/ds¹ is an enhancer of phenotype of fz²⁴ (Adler et al., 1998) ds^33k is an enhancer of phenotype of fz²⁴ (Adler et al., 1998)
NOT Enhancer of
Statement Reference ds^33k/ds[+] is a non-enhancer of wing vein phenotype of sog^EP7 (Araujo et al., 2003) ds^33k/ds[+] is a non-enhancer of wing vein phenotype of sog^EP11 (Araujo et al., 2003)
NOT Suppressor of
Statement Reference ds^33k/ds[+] is a non-suppressor of wing vein phenotype of sog^EP7 (Araujo et al., 2003) ds^33k/ds[+] is a non-suppressor of wing vein phenotype of sog^EP11 (Araujo et al., 2003)
Additional Comments
Genetic Interactions
Statement Reference The distinctive ds polarity phenotype of ds^33k/ds^UAO71 flies (regions of reversed polarity on the wing) is suppressed by expression of fz^I.hs 24 hours after white prepupa formation using a 1 hour heat shock. The appendage shape abnormalities and wing vein placement abnormalities are not suppressed. The fraction of the wing that shows abnormal polarity in fz²⁴ flies is increased if the fly also carries ds¹/ds^33k. The domineering nonautonomy of fz¹ clones is enhanced by ds^UAO71/ds^33k. (Adler et al., 1998)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Fails to complement	ds^UAO71 (Adler et al., 1998)
Comments
Stocks ( 37 )
Bloomington	1580 In(2LR)bw^V1, ds^33k bw^V1/SM5 390 S²/In(2LR)bw^V1, ds^33k bw^V1 4525 Ruf¹/In(2LR)bw^V1, ds^33k bw^V1 224 Df(2R)cn87e/In(2LR)bw^V1, ds^33k b¹ bw^V1 351 mr¹ bs²/In(2LR)bw^V1, ds^33k bw^V1 3170 In(2LR)bw^V1, ds^33k l(2)DTS18WF¹ bw^V1/CyO
Kyoto	106399 In(2LR)bw^V1, ds^33k bw^V1/SM5 105885 S²/In(2LR)bw^V1, ds^33k bw^V1 105798 Df(2R)cn87e/In(2LR)bw^V1, ds^33k b¹ bw^V1 101501 In(2L)Cy, Cy¹ / In(2LR)bw^V1, ds^33k bw^V1 105865 mr¹ bs²/In(2LR)bw^V1, ds^33k bw^V1 106891 In(2LR)bw^V1, ds^33k l(2)DTS18WF¹ bw^V1/CyO
	More stocks available...
Notes on Origin
Discoverer	Bridges, 28th Nov. 1933. Bridges.

Comments
	Strong allele. (Clark et al., 1995) Analysis of ds^33k/ds^UAO71 clones shows that ds displays domineering cell nonautonomy. (Adler et al., 1998)
Synonyms & Secondary IDs ( 2 )
Reported As
Symbol Synonym	ds^33K (Adler et al., 1998) ds^33k
Name Synonym
Secondary FlyBase IDs

References ( 9 )
Research paper	Dearborn and Kunes, 2004, Development 131(10): 2291--2303 An axon scaffold induced by retinal axons directs glia to destinations in the Drosophila optic lobe. [FBrf0174580] Rodriguez, 2004, Development 131(13): 3195--3206 The dachsous gene, a member of the cadherin family, is required for Wg-dependent pattern formation in the Drosophila wing disc. [FBrf0179415] Araujo et al., 2003, Development 130(16): 3851--3864 Integrins modulate Sog activity in the Drosophila wing. [FBrf0160375] Strutt and Strutt, 2002, Dev. Cell 3(6): 851--863 Nonautonomous planar polarity patterning in Drosophila: dishevelled-independent functions of frizzled. [FBrf0155678] Adler et al., 1998, Development 125(5): 959--968 Mutations in the cadherin superfamily member gene dachsous cause a tissue polarity phenotype by altering frizzled signaling. [FBrf0101864] Clark et al., 1995, Genes Dev. 9(12): 1530--1542 Dachsous encodes a member of the cadherin superfamily that controls imaginal disc morphogenesis in Drosophila. [FBrf0081626] Lederman-Klein, 1953, D. I. S. 27: 57 [New mutants report.] [FBrf0095554] Bridges, 1935, D. I. S. 4: 5--13 Drosophila melanogaster. Symbols, characteristics of mutants, valuations. Corrections and supplement to list in D. I. S. 3: 5--19. [FBrf0063326]
Book	Lindsley and Grell, 1968, Publs Carnegie Instn 627: 469pp Genetic variations of Drosophila melanogaster. [FBrf0020044]

Allele Dmel\ds33k

Allele Dmel\ds^33k