|Feature type||allele||Associated gene||Dmel\ey|
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|Nature of the Allele|
|Mutations Mapped to the Genome|
|Associated Sequence Data|
|Nature of the lesion|
A piece of the 2nd chromosome causes Dp(2;4)eyD by inserting into exon 5 of the ey gene replacing 320bp of ey sequence. This causes a truncated ey protein (346 aa) to be produced. Its 32 C terminal amino acids are coded by the inserted sequences of the second chromosome. There is also a 327bp insertion close to the distal end of the duplication - within the ey gene.
|Caused by aberration|
|Phenotype Manifest In|
Homozygous ey[D] mutant escaper adults are headless. Eye-antennal discs dissected from ey[D] homozygous larvae exhibit massive cell death.
About half of eyD homozygous pharate adults exhibit a headless phenotype, missing all or most structures derived from the eye-antennal disc. ey2/eyD and eyR/eyD animals have small eyes.
Homozygotes show a headless phenotype.
Geotaxis score no different from Canton S in a Hirsch-type geotaxis maze.
Adult eyes vary in size from full to nothing, eye disc varies from rudimentary to full-sized, due to precursor cell defects.
Eyes are greatly reduced in size.
Eye1 and eyD show an additive effect with respect to eye phenotype; Eye1/+; eyD/+ shows more marked eyelessness than either single heterozygote.
RK2. Eyes of heterozygotes small, outline irregular, displaced toward top and rear. Head large, often with duplicated antennae or ocelli. Fully dominant in triplo-4 flies (Sturtevant, 1936). Eye size of B; eyD/+ males larger than of B alone. Produces extreme phenotype in combination with D. D/+; eyD/+ almost completely lethal (Sobels, Kruijt and Spronk, 1951). Homozygous lethal; two lethal crises, one during first or second larval instar and the other just prior to or during pupal stage. Cell degeneration observed in optic discs of homozygous second-instar larval (Ransom, 1979). Larvae which are unable to pupate rescuable by injection of α-ecdysone (Arking, 1969). Homozygotes reaching pupal stage lack adult derivatives of eye-antennal discs; adult derivatives are formed by eyD/eyD eye-antennal discs transplanted into wild-type hosts; brain present but number of cortical cells severely reduced (Arking, Putnam, and Schubiger, 1975).
eyD has increased cell death | larval stage phenotype, suppressible by Scer\GAL4toy2/BacA\p35Scer\UAS.cHa
|Phenotype Manifest In|
|NOT suppressed by|
Expression of toy[Scer\UAS.cCa] under the control of Scer\GAL4[toy1] significantly suppresses the lethality seen in ey[D] mutants, raising the fraction of homozygous adults from 0.27% to 7.3%. The morphology of compound eyes in these flies is also improved by targeted expression of toy[Scer\UAS.cCa], but never to wild-type proportions. A small eye phenotype is found in 76% of rescued flies.
Expression of BacA\p35[Scer\UAS.cHa] under the control of Scer\GAL4[toy2] significantly increases the survival of toy[hdl] homozygous mutants to the adult stage. The head structure defects in these rescued flies, apart from the compound eyes, are also improved.
|Complementation & Rescue Data|
Expression of ey[Scer\UAS.cHa] under the control of Scer\GAL4[ey.PH] significantly rescues the lethality seen in ey[D] homozygous mutants. The flies that eclose from pupae exhibit smaller than normal heads and a majority of the compound eyes are reduced in size, while the ocelli are normal. The viability of these flies is more efficient at 18[o]C compared to 25[o]C and 29[o]C. The rescue efficiencies of flies carrying both driver and effector constructs or effector construct alone (i.e only ey[Scer\UAS.cHa]) are similar.
|Stocks ( 27 )|
|Notes on Origin|
Muller, Nov. 1927.
|External Crossreferences & Linkouts|
|Synonyms & Secondary IDs ( 2 )|
|Secondary FlyBase IDs|
|References ( 27 )|
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|Recent research papers (0)|
|All research papers listed in FlyBase were published before 2011|