Allele Dmel\ey^D

General Information
Symbol	Dmel\ey^D	Species	D. melanogaster
Name	Dominant	FlyBase ID	FBal0028184
Feature type	allele	Associated gene	Dmel\ey

Allele class
Mutagen	X ray
Recent Updates
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FB2013_03
FB2013_02
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Nature of the Allele
Allele class
Mutagen	X ray
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference A piece of the 2nd chromosome causes Dp(2;4)ey^D by inserting into exon 5 of the ey gene replacing 320bp of ey sequence. This causes a truncated ey protein (346 aa) to be produced. Its 32 C terminal amino acids are coded by the inserted sequences of the second chromosome. There is also a 327bp insertion close to the distal end of the duplication - within the ey gene. (Kronhamn et al., 2002)
Caused by aberration	Dp(2;4)ey^D (Kronhamn et al., 2002, Hochman et al., 1964)
Cytology
Phenotypic Data
Phenotypic Class
	increased cell death \| larval stage (Jacobsson et al., 2009) lethal \| pupal stage (Jacobsson et al., 2009) lethal \| recessive (Kronhamn et al., 2002, Hochman et al., 1964, Renfranz and Benzer, 1989) visible (Jiao et al., 2001)
Phenotype Manifest In
	adult head (Jiao et al., 2001, Jacobsson et al., 2009) eye (Hochman et al., 1964, Jacobsson et al., 2009) eye (with ey²) (Jang et al., 2003, Kronhamn et al., 2002) eye (with ey^R) (Kronhamn et al., 2002) eye-antennal disc (Kronhamn et al., 2002, Jacobsson et al., 2009) head (Kronhamn et al., 2002)
Detailed Description
	Statement Reference Homozygous ey[D] mutant escaper adults are headless. Eye-antennal discs dissected from ey[D] homozygous larvae exhibit massive cell death. (Jacobsson et al., 2009) ey^D/ey² animals have no eyes and is nearly completely penetrant. (Jang et al., 2003) About half of ey^D homozygous pharate adults exhibit a headless phenotype, missing all or most structures derived from the eye-antennal disc. ey²/ey^D and ey^R/ey^D animals have small eyes. (Kronhamn et al., 2002) Homozygotes show a headless phenotype. (Jiao et al., 2001) Geotaxis score no different from Canton S in a Hirsch-type geotaxis maze. (McMillan and McGuire, 1992) Adult eyes vary in size from full to nothing, eye disc varies from rudimentary to full-sized, due to precursor cell defects. (Renfranz and Benzer, 1989) Eyes are greatly reduced in size. (Hochman et al., 1964) Eye¹ and ey^D show an additive effect with respect to eye phenotype; Eye¹/+; ey^D/+ shows more marked eyelessness than either single heterozygote. (Edmondson, 1952) RK2. Eyes of heterozygotes small, outline irregular, displaced toward top and rear. Head large, often with duplicated antennae or ocelli. Fully dominant in triplo-4 flies (Sturtevant, 1936). Eye size of B; ey^D/+ males larger than of B alone. Produces extreme phenotype in combination with D. D/+; ey^D/+ almost completely lethal (Sobels, Kruijt and Spronk, 1951). Homozygous lethal; two lethal crises, one during first or second larval instar and the other just prior to or during pupal stage. Cell degeneration observed in optic discs of homozygous second-instar larval (Ransom, 1979). Larvae which are unable to pupate rescuable by injection of α-ecdysone (Arking, 1969). Homozygotes reaching pupal stage lack adult derivatives of eye-antennal discs; adult derivatives are formed by ey^D/ey^D eye-antennal discs transplanted into wild-type hosts; brain present but number of cortical cells severely reduced (Arking, Putnam, and Schubiger, 1975).
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
Statement Reference ey^D has increased cell death \| larval stage phenotype, suppressible by Scer\GAL4^toy2/BacA\p35^Scer\UAS.cHa (Jacobsson et al., 2009) ey^D has lethal \| pupal stage phenotype, suppressible by Scer\GAL4^toy2/BacA\p35^Scer\UAS.cHa (Jacobsson et al., 2009) ey^D has lethal \| pupal stage phenotype, suppressible by toy^Scer\UAS.cCa/Scer\GAL4^toy2 (Jacobsson et al., 2009)
Phenotype Manifest In
Suppressed by
Statement Reference ey^D/ey² has eye phenotype, suppressible \| partially by Scer\GAL4^dpp.blk1/eyg^{Scer\UAS.lune} (Jang et al., 2003) ey^D has adult head phenotype, suppressible by Scer\GAL4^toy2/BacA\p35^Scer\UAS.cHa (Jacobsson et al., 2009) ey^D has adult head phenotype, suppressible by toy^Scer\UAS.cCa/Scer\GAL4^toy2 (Jacobsson et al., 2009) ey^D has eye-antennal disc phenotype, suppressible by Scer\GAL4^ey.PH/BacA\p35^Scer\UAS.cHa (Kronhamn et al., 2002) ey^D has eye-antennal disc phenotype, suppressible by toy^Scer\UAS.cCa/Scer\GAL4^toy2 (Jacobsson et al., 2009) ey^D has eye phenotype, suppressible by Scer\GAL4^toy2/BacA\p35^Scer\UAS.cHa (Jacobsson et al., 2009) ey^D has eye phenotype, suppressible by toy^Scer\UAS.cCa/Scer\GAL4^toy2 (Jacobsson et al., 2009) ey^D has head phenotype, suppressible by Scer\GAL4^ey.PH/BacA\p35^Scer\UAS.cHa (Kronhamn et al., 2002)
NOT suppressed by
Statement Reference ey^D has phenotype, non-suppressible by su(Hw)² (Lindsley and Grell, 1968)
Other
Statement Reference BacA\p35^Scer\UAS.cHa, Scer\GAL4^ey.PH, ey^D has eye phenotype (Kronhamn et al., 2002, Kronhamn et al., 2002)
Additional Comments
Genetic Interactions
Statement Reference Expression of toy[Scer\UAS.cCa] under the control of Scer\GAL4[toy1] significantly suppresses the lethality seen in ey[D] mutants, raising the fraction of homozygous adults from 0.27% to 7.3%. The morphology of compound eyes in these flies is also improved by targeted expression of toy[Scer\UAS.cCa], but never to wild-type proportions. A small eye phenotype is found in 76% of rescued flies. (Jacobsson et al., 2009) The addition of BacA\p35^Scer\UAS.cHa driven by Scer\GAL4^ey.PH more than half of the flies are rescued to viable adults that are eyeless, but have developed most other head structures derived from the eye-antennal discs, usually including all three ocelli. (Kronhamn et al., 2002)
Xenogenetic Interactions
Statement Reference Expression of BacA\p35[Scer\UAS.cHa] under the control of Scer\GAL4[toy2] significantly increases the survival of toy[hdl] homozygous mutants to the adult stage. The head structure defects in these rescued flies, apart from the compound eyes, are also improved. (Jacobsson et al., 2009) The addition of BacA\p35^Scer\UAS.cHa driven by Scer\GAL4^ey.PH rescues more than half of the flies to viable adults that are eyeless, but have developed most other head structures derived from the eye-antennal discs, usually including all three ocelli. (Kronhamn et al., 2002)
Complementation & Rescue Data
Rescued by	ey^D is rescued by ey^Scer\UAS.cHa/Scer\GAL4[-] (Jacobsson et al., 2009) ey^D is rescued by ey^Scer\UAS.cHa/Scer\GAL4^ey.PH (Jacobsson et al., 2009)
Comments	Expression of ey[Scer\UAS.cHa] under the control of Scer\GAL4[ey.PH] significantly rescues the lethality seen in ey[D] homozygous mutants. The flies that eclose from pupae exhibit smaller than normal heads and a majority of the compound eyes are reduced in size, while the ocelli are normal. The viability of these flies is more efficient at 18[o]C compared to 25[o]C and 29[o]C. The rescue efficiencies of flies carrying both driver and effector constructs or effector construct alone (i.e only ey[Scer\UAS.cHa]) are similar. (Jacobsson et al., 2009) Dp(2;4)ey^D chromosome is also associated with a dominant gain of function mutation, Alp^eyD (FBal0027957), which is responsible for the leg phenotype previously associated with ey^D. (Cohen, 1995.4.19)
Stocks ( 27 )
Bloomington	13697 y¹; P{SUPor-P}KG01127/ey^D 14895 y¹; P{SUPor-P}pan^KG01431/ey^D 4227 ar¹/Dp(2;4)ey^D, Alp^eyD: ey^D 662 sv^de/Dp(2;4)ey^D, Alp^eyD: ey^D 1122 Scn¹/Dp(2;4)ey^D, Alp^eyD: ey^D 4760 pan³/Dp(2;4)ey^D, Alp^eyD: ey^D 4043 pan^13a/Dp(2;4)ey^D, Alp^eyD: ey^D 4343 ci^Ce-2/Dp(2;4)ey^D, Alp^eyD: ey^D
Kyoto	106212 Scn¹/Dp(2;4)ey^D, Alp^eyD: ey^D 107860 pan²/Dp(2;4)ey^D, Alp^eyD: ey^D 107565 pan^13a/Dp(2;4)ey^D, Alp^eyD: ey^D 103322 Df(4)M101-62f / Dp(2;4)ey^D, Alp^eyD ; ey^D
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Notes on Origin
Discoverer	Muller, Nov. 1927.

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 2 )
Reported As
Symbol Synonym	ey^D (Jacobsson et al., 2009)
Name Synonym	Dominant
Secondary FlyBase IDs

References ( 27 )

Generate a list of	All references relating to this allele ( 27 )

List References by type	Research paper ( 22 ) Personal communication to FlyBase ( 2 ) Book ( 2 ) Stock list ( 1 )
Recent research papers (0)
	All research papers listed in FlyBase were published before 2011 Show research papers ...

Allele Dmel\eyD

Allele Dmel\ey^D