Allele Dmel\faf^BX4

General Information
Symbol	Dmel\faf^BX4	Species	D. melanogaster
Name		FlyBase ID	FBal0028189
Feature type	allele	Associated gene	Dmel\faf

Allele class	amorphic allele - genetic evidence, loss of function allele
Mutagen	X ray
Recent Updates
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FB2013_03
FB2013_02
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Nature of the Allele
Allele class	amorphic allele - genetic evidence (Fischer-Vize et al., 1992) loss of function allele (Isaksson et al., 1997)
Mutagen	X ray (Fischer-Vize et al., 1992)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Inversion breakpoint in exon 3. (Chen and Fischer, 2000) Inversion breakpoint falls in coding region of faf. (Fischer-Vize et al., 1992)
Caused by aberration	In(3R)faf^BX4 (Chen and Fischer, 2000, Fischer-Vize et al., 1992, Chen et al., 2002)
Cytology
Phenotypic Data
Phenotypic Class
	female sterile \| recessive (Fischer-Vize et al., 1992) viable (Isaksson et al., 1997) visible (Huang and Fischer-Vize, 1996) visible (with faf^FO8) (Chen et al., 2000, Chen and Fischer, 2000) visible \| recessive (Fischer-Vize et al., 1992)
Phenotype Manifest In
	eye (Huang et al., 1995) eye (with faf^FO8) (Chen and Fischer, 2000) eye photoreceptor cell (Li et al., 1997) eye photoreceptor cell (with faf^FO8) (Chen et al., 2000) eye photoreceptor cell & ommatidium \| ectopic \| somatic clone (Overstreet et al., 2004) eye photoreceptor cell \| supernumerary (with faf^FO8) (Chen and Fischer, 2000) ommatidium (Huang and Fischer-Vize, 1996) ommatidium (with faf^FO8) (Chen et al., 2000, Chen and Fischer, 2000) photoreceptor cell R1 \| ectopic (Isaksson et al., 1997) photoreceptor cell R2 \| ectopic (Isaksson et al., 1997) photoreceptor cell R3 \| ectopic (Isaksson et al., 1997) photoreceptor cell R4 \| ectopic (Isaksson et al., 1997) photoreceptor cell R5 \| ectopic (Isaksson et al., 1997) photoreceptor cell R6 \| ectopic (Isaksson et al., 1997)
Detailed Description
	Statement Reference faf^BX4 homozygous somatic clones in the eye lead to the formation of ommatidia containing one additional photoreceptor cell. For an ommatidium to show this phenotype, it is sufficient for 2 of the photoreceptors R2 to R5 to be mutant, but no particular cell of this group need be. (Overstreet et al., 2004) The external surface of the eye is irregular in faf^FO8/faf^BX4 flies and most facets contain more than the wild-type number of photoreceptor cells. (Chen and Fischer, 2000) BrdU incorporation resembles wild type in faf^FO8/faf^BX4 third larval instar eye discs. (Chen et al., 2000) faf^BX4/faf^FO8 flies have a rough eye phenotype, due to the ommatidia containing more than the normal number of photoreceptor cells. (Chen et al., 2000) Most facets of homozygotes contain more than the normal complement of 8 photoreceptor cells, many facets have extra R7 cells and a small proportion have missing R7 cells. (Li et al., 1997) Mutation causes no significant alteration to the variegated eye phenotype of In(1)w^m4h. (Henchoz et al., 1996) Average fraction of wild type facets is ~4.5%. (Huang et al., 1995) Eyes roughened due to abnormalities in the hexagonal facet array: most facets include 1-3 extra outer photoreceptor cells which are probably mystery cells. Ovaries of homozygous females are normal in appearance but the embryos produced never form syncytial blastoderm and all fail to hatch. (Fischer-Vize et al., 1992)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement Reference faf^BX4 has visible phenotype, enhanceable by aop^yan-1/aop[+] (Isaksson et al., 1997)
Suppressed by
Statement Reference faf^BX4 has visible phenotype, suppressible by 14-3-3ζ^E16/14-3-3zeta[+] (Isaksson et al., 1997) faf^BX4 has visible phenotype, suppressible by Jra^IA109/Jra[+] (Isaksson et al., 1997) faf^BX4 has visible phenotype, suppressible by pnt[+]/pnt^Δ88 (Isaksson et al., 1997) faf^BX4 has visible phenotype, suppressible by pnt¹²⁷⁷ (Isaksson et al., 1997) faf^FO8/faf^BX4 has visible phenotype, suppressible by Mmus\Usp9x^-.ro (Chen et al., 2000) faf^FO8/faf^BX4 has visible phenotype, suppressible by Mmus\Usp9x^+.ro (Chen et al., 2000)
NOT Enhancer of
Statement Reference faf^BX4 is a non-enhancer of visible phenotype of Ppt1^Scer\UAS.cKa, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF (Buff et al., 2007)
NOT Suppressor of
Statement Reference faf^BX4 is a non-suppressor of visible phenotype of Ppt1^Scer\UAS.cKa, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF (Buff et al., 2007)
Other
Statement Reference faf^BX4, sev¹⁴ has visible phenotype (Isaksson et al., 1997)
Phenotype Manifest In
Enhanced by
Statement Reference faf^BX4 has photoreceptor cell R1 \| ectopic phenotype, enhanceable by aop^yan-1/aop[+] (Isaksson et al., 1997) faf^BX4 has photoreceptor cell R2 \| ectopic phenotype, enhanceable by aop^yan-1/aop[+] (Isaksson et al., 1997) faf^BX4 has photoreceptor cell R3 \| ectopic phenotype, enhanceable by aop^yan-1/aop[+] (Isaksson et al., 1997) faf^BX4 has photoreceptor cell R4 \| ectopic phenotype, enhanceable by aop^yan-1/aop[+] (Isaksson et al., 1997) faf^BX4 has photoreceptor cell R5 \| ectopic phenotype, enhanceable by aop^yan-1/aop[+] (Isaksson et al., 1997) faf^BX4 has photoreceptor cell R6 \| ectopic phenotype, enhanceable by aop^yan-1/aop[+] (Isaksson et al., 1997)
Suppressed by
Statement Reference faf^BX4 has phenotype, suppressible \| partially by Scer\GAL4^h-1J3 (Huang and Fischer-Vize, 1996) faf^BX4 has phenotype, suppressible \| partially by Scer\GAL4^h-H10 (Huang and Fischer-Vize, 1996) faf^BX4 has photoreceptor cell R1 \| ectopic phenotype, suppressible by 14-3-3ζ^E16/14-3-3zeta[+] (Isaksson et al., 1997) faf^BX4 has photoreceptor cell R1 \| ectopic phenotype, suppressible by Jra^IA109/Jra[+] (Isaksson et al., 1997) faf^BX4 has photoreceptor cell R1 \| ectopic phenotype, suppressible by pnt[+]/pnt^Δ88 (Isaksson et al., 1997) faf^BX4 has photoreceptor cell R1 \| ectopic phenotype, suppressible by pnt¹²⁷⁷ (Isaksson et al., 1997) faf^BX4 has photoreceptor cell R2 \| ectopic phenotype, suppressible by 14-3-3ζ^E16/14-3-3zeta[+] (Isaksson et al., 1997) faf^BX4 has photoreceptor cell R2 \| ectopic phenotype, suppressible by Jra^IA109/Jra[+] (Isaksson et al., 1997) faf^BX4 has photoreceptor cell R2 \| ectopic phenotype, suppressible by pnt[+]/pnt^Δ88 (Isaksson et al., 1997) faf^BX4 has photoreceptor cell R2 \| ectopic phenotype, suppressible by pnt¹²⁷⁷ (Isaksson et al., 1997) faf^BX4 has photoreceptor cell R3 \| ectopic phenotype, suppressible by 14-3-3ζ^E16/14-3-3zeta[+] (Isaksson et al., 1997) faf^BX4 has photoreceptor cell R3 \| ectopic phenotype, suppressible by Jra^IA109/Jra[+] (Isaksson et al., 1997) faf^BX4 has photoreceptor cell R3 \| ectopic phenotype, suppressible by pnt[+]/pnt^Δ88 (Isaksson et al., 1997) faf^BX4 has photoreceptor cell R3 \| ectopic phenotype, suppressible by pnt¹²⁷⁷ (Isaksson et al., 1997) faf^BX4 has photoreceptor cell R4 \| ectopic phenotype, suppressible by 14-3-3ζ^E16/14-3-3zeta[+] (Isaksson et al., 1997) faf^BX4 has photoreceptor cell R4 \| ectopic phenotype, suppressible by Jra^IA109/Jra[+] (Isaksson et al., 1997) faf^BX4 has photoreceptor cell R4 \| ectopic phenotype, suppressible by pnt[+]/pnt^Δ88 (Isaksson et al., 1997) faf^BX4 has photoreceptor cell R4 \| ectopic phenotype, suppressible by pnt¹²⁷⁷ (Isaksson et al., 1997) faf^BX4 has photoreceptor cell R5 \| ectopic phenotype, suppressible by 14-3-3ζ^E16/14-3-3zeta[+] (Isaksson et al., 1997) faf^BX4 has photoreceptor cell R5 \| ectopic phenotype, suppressible by Jra^IA109/Jra[+] (Isaksson et al., 1997) faf^BX4 has photoreceptor cell R5 \| ectopic phenotype, suppressible by pnt[+]/pnt^Δ88 (Isaksson et al., 1997) faf^BX4 has photoreceptor cell R5 \| ectopic phenotype, suppressible by pnt¹²⁷⁷ (Isaksson et al., 1997) faf^BX4 has photoreceptor cell R6 \| ectopic phenotype, suppressible by 14-3-3ζ^E16 (Isaksson et al., 1997) faf^BX4 has photoreceptor cell R6 \| ectopic phenotype, suppressible by Jra^IA109/Jra[+] (Isaksson et al., 1997) faf^BX4 has photoreceptor cell R6 \| ectopic phenotype, suppressible by pnt¹²⁷⁷ (Isaksson et al., 1997) faf^BX4 has photoreceptor cell R6 \| ectopic phenotype, suppressible by pnt^Δ88 (Isaksson et al., 1997) faf^FO8/faf^BX4 has eye phenotype, suppressible by lqf^+t19G (Cadavid et al., 2000) faf^FO8/faf^BX4 has eye phenotype, suppressible by lqf^{ro.T:Zzzz\FLAG} (Cadavid et al., 2000) faf^FO8/faf^BX4 has ommatidium phenotype, suppressible by Mmus\Usp9x^-.ro (Chen et al., 2000) faf^FO8/faf^BX4 has ommatidium phenotype, suppressible by Mmus\Usp9x^+.ro (Chen et al., 2000)
NOT Enhancer of
Statement Reference faf^BX4 is a non-enhancer of eye phenotype of Ppt1^Scer\UAS.cKa, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF (Buff et al., 2007)
Suppressor of
Statement Reference faf^BX4 is a suppressor of synaptic vesicle phenotype of hiw^unspecified (DiAntonio et al., 2001) faf^FO8/faf^BX4 is a suppressor of synaptic vesicle phenotype of hiw^unspecified (DiAntonio et al., 2001)
NOT Suppressor of
Statement Reference faf^BX4 is a non-suppressor of eye phenotype of Ppt1^Scer\UAS.cKa, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF (Buff et al., 2007) faf^BX4 is a non-suppressor of synapse phenotype of hiw^unspecified (DiAntonio et al., 2001) faf^FO8/faf^BX4 is a non-suppressor of synapse phenotype of hiw^unspecified (DiAntonio et al., 2001)
Other
Statement Reference faf^BX4, sev¹⁴ has photoreceptor cell R7 phenotype (Isaksson et al., 1997)
Additional Comments
Genetic Interactions
Statement Reference The addition of either homozygous faf^BX4, or faf^BX4/faf^FO8 suppresses the physiological phenotype of hiw^unspecified, leading to a more tha doubling of both quantal content and mEJP frequency. However the synaptic overgrowth phenotype is not affected. (DiAntonio et al., 2001) The faf^FO8/faf^BX4 eye phenotype is completely suppressed by one copy of lqf^+t19G or lqf^{ro.T:Zzzz\FLAG}. (Cadavid et al., 2000) The extra outer photoreceptor cell phenotype is suppressed by pnt¹²⁷⁷: faf^BX4 ; pnt¹²⁷⁷ double homozygotes have an average of 6.2 outer photoreceptor cells per ommatidium. The extra outer photoreceptor cell phenotype is dominantly suppressed by pnt^Δ88, by 14-3-3ζ^E16 and also by Jra^IA109. The extra outer photoreceptor cell phenotype is dominantly enhanced by aop^yan-1: aop^yan-1/+ ; faf^BX4/faf^BX4 flies have an average of 8.7 outer photoreceptor cells per ommatidium. Does not alter the outer photoreceptor cell phenotype of phl¹²/Y males. 60% of sev¹⁴; faf^BX4 double homozygous ommatidia have an R7 cell. (Isaksson et al., 1997) Suppression appears to be due to the presence of Scer\GAL4 protein ahead of the furrow. (Huang and Fischer-Vize, 1996)
Xenogenetic Interactions
Statement Reference Mmus\Usp9x^+.ro or Mmus\Usp9x^-.ro rescue nearly completely the eye phenotype of faf^BX4/faf^FO8 flies. (Chen et al., 2000)
Complementation & Rescue Data
Fails to complement	faf^EP381.faf- (DiAntonio et al., 2001)
Rescued by	faf^BX4 is rescued by faf^+t13 (Fischer-Vize et al., 1992, Huang et al., 1995) faf^BX4 is rescued by faf^+t14 (Fischer-Vize et al., 1992) faf^BX4 is rescued by faf^+t17 (Fischer-Vize et al., 1992) faf^BX4 is rescued by faf^{hs.T:Hsap\MYC} (Huang and Fischer-Vize, 1996) faf^BX4 is rescued by faf^{ro.T:Hsap\MYC} (Huang and Fischer-Vize, 1996) faf^FO8/faf^BX4 is rescued by faf^{ro.T:Hsap\MYC} (Chen and Fischer, 2000)
Partially rescued by	faf^BX4 is partially rescued by faf^glrs (Huang and Fischer-Vize, 1996) faf^BX4 is partially rescued by faf^{hs.T:Hsap\MYC} (Huang et al., 1995) faf^FO8/faf^BX4 is partially rescued by faf^{Δ1.ro.T:Hsap\MYC} (Chen and Fischer, 2000) faf^FO8/faf^BX4 is partially rescued by faf^{Δ2.ro.T:Hsap\MYC} (Chen and Fischer, 2000) faf^FO8/faf^BX4 is partially rescued by faf^{Δ3.ro.T:Hsap\MYC} (Chen and Fischer, 2000) faf^FO8/faf^BX4 is partially rescued by faf^{Δ4.ro.T:Hsap\MYC} (Chen and Fischer, 2000) faf^FO8/faf^BX4 is partially rescued by faf^{Δ5.ro.T:Hsap\MYC} (Chen and Fischer, 2000) faf^FO8/faf^BX4 is partially rescued by faf^{Δ6.ro.T:Hsap\MYC} (Chen and Fischer, 2000)
Not rescued by	faf^BX4 is not rescued by faf^glrl (Huang and Fischer-Vize, 1996) faf^BX4 is not rescued by faf^Scer\UAS.cHa/Scer\GAL4^h-1J3 (Huang and Fischer-Vize, 1996) faf^BX4 is not rescued by faf^Scer\UAS.cHa/Scer\GAL4^sca-4512 (Huang and Fischer-Vize, 1996) faf^BX4 is not rescued by faf^Scer\UAS.cHa/Scer\GAL4^sca-T3 (Huang and Fischer-Vize, 1996) faf^BX4 is not rescued by faf^sev.PH (Huang and Fischer-Vize, 1996) faf^BX4 is not rescued by Scer\GAL4^elav-C155/faf^Scer\UAS.cHa (Huang and Fischer-Vize, 1996)
Comments	faf^{hs.T:Hsap\MYC} and faf^{ro.T:Hsap\MYC} can nearly completely rescue the eye phenotype, faf^glrs partially rescues and, faf^glrl and faf^sev.PH fail to rescue. (Huang and Fischer-Vize, 1996)
Stocks ( 2 )
Bloomington	25107 w^; st¹ faf^BX4/TM6B, Tb¹ 25102 w^; P{UAS-faf.H}2; st¹ faf^BX4/TM6B, Tb¹
Notes on Origin
Discoverer

Comments
	Very weak faf allele. (Huang and Fischer-Vize, 1996) No interaction with P{sev-svp1} or P{sev-svp2} exists. (Begemann et al., 1995)
External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 1 )
Reported As
Symbol Synonym	faf^BX4 (Buff et al., 2007)
Name Synonym
Secondary FlyBase IDs

References ( 15 )
Research paper	Buff et al., 2007, Genetics 176(1): 209--220 Genetic modifiers of Drosophila palmitoyl-protein thioesterase 1-induced degeneration. [FBrf0201672] Overstreet et al., 2004, Development 131(21): 5355--5366 Fat facets and Liquid facets promote Delta endocytosis and Delta signaling in the signaling cells. [FBrf0180167] Chen et al., 2002, Genes Dev. 16(3): 289--294 A specific protein substrate for a deubiquitinating enzyme: liquid facets is the substrate of Fat facets. [FBrf0144879] DiAntonio et al., 2001, Nature 412(6845): 449--452 Ubiquitination-dependent mechanisms regulate synaptic growth and function. [FBrf0137327] Cadavid et al., 2000, Development 127(8): 1727--1736 The function of the Drosophila fat facets deubiquitinating enzyme in limiting photoreceptor cell number is intimately associated with endocytosis. [FBrf0127031] Chen and Fischer, 2000, Genetics 156(4): 1829--1836 In vivo structure/function analysis of the Drosophila fat facets deubiquitinating enzyme gene. [FBrf0131273] Chen et al., 2000, Dev. Genes Evol. 210(12): 603--610 On the conservation of function of the Drosophila Fat facets deubiquitinating enzyme and Fam, its mouse homolog. [FBrf0132290] Chen et al., 2000, Genetics 156(4): 1787--1795 Genetic analysis of the Drosophila DNAprim gene: The function of the 60-kd primase subunit of DNA polymerase opposes the fat facets signaling pathway in the developing eye. [FBrf0131274] Isaksson et al., 1997, Mech. Dev. 68(1,2): 59--67 The deubiquitination enzyme fat facets negatively regulates RTK/Ras/MAPK signalling during Drosophila eye development. [FBrf0099843] Li et al., 1997, Proc. Natl. Acad. Sci. U.S.A. 94(23): 12515--12520 Genetic interactions with rap1 and ras1 reveal a second function for the fat facets deubiquitinating enzyme in Drosophila eye development. [FBrf0099363] Henchoz et al., 1996, Mol. Cell. Biol. 16(10): 5717--5725 The dose of a putative ubiquitin-specific protease affects position-effect variegation in Drosophila melanogaster. [FBrf0090605] Huang and Fischer-Vize, 1996, Development 122(10): 3207--3216 Undifferentiated cells in the developing Drosophila eye influence facet assembly and require the Fat facets ubiquitin-specific protease. [FBrf0090618] Begemann et al., 1995, Development 121(1): 225--235 The Drosophila orphan nuclear receptor Seven-up requires the Ras pathway for its function in photoreceptor determination. [FBrf0076133] Huang et al., 1995, Science 270(5243): 1828--1831 Control of cell fate by a deubiquitinating enzyme encoded by the fat facets gene. [FBrf0085249] Fischer-Vize et al., 1992, Development 116: 985--1000 The fat facets gene is required for Drosophila eye and embryo development. [FBrf0055917]

Allele Dmel\fafBX4

Allele Dmel\faf^BX4