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General Information
Symbol
Dmel\Gem3rL562
Species
D. melanogaster
Name
FlyBase ID
FBal0028393
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Key Links
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

Insertion located immediately downstream of the translation start site.

Insertion 108bp downstream of the transcription start site.

Insertion components
P{PZ}Gem3rL562
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

15% of homozygous larvae survive to day 8 after egg laying (a period when the control larvae have already pupated) but fail to pupate. A small fraction of homozygous larvae survive to day 25 after egg laying.

Homozygous larvae are thinner and shorter than control larvae at 4 days after egg laying. The mouth hooks of these animals are more similar to those of wild-type second instar larvae, whereas control animals at this time have entered the third larval instar stage.

Homozygous second-third instar larvae show impaired motor function, showing a smaller distance travelled in the 20 seconds after movement was stimulated with a needle than control larvae.

Homozygotes survive to the third instar larval stage, while Gem3rL562/Gem3e03688 animals survive to the prepupal stage. Both genotypes undergo a prolonged wandering third instar larval stage.

Gem3rL562/Gem3e03688 animals show normal body wall contraction rates throughout the first and second larval instars, but at the third instar larval stage the body wall contraction rate is significantly decreased in the mutant animals compared to controls. The mutant third instar larvae show no apparent disruption in the gross structure of the body wall musculature or in sarcomere organisation.

The puparium made by Gem3rL562/Gem3e03688 animals fails to evert spiracles and has a large axial ratio (length/width).

Gem3rL562/Gem3e03688 larvae show a synaptic overgrowth phenotype at the neuromuscular junction (NMJ) and have a significantly increased synaptic area when it is normalised to muscle size.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
NOT suppressed by
Statement
Reference
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments

Expression of Gem3Scer\UAS.T:Avic\GFP-CFP under the control of Scer\GAL41032.hx rescues the mobility, spiracle eversion and abnormal axial ratio phenotypes seen in Gem3[rL562]/Gem3[e03688] mutants.

Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer

G. Rubin.

Comments
Comments

Reversion analysis proved that the P{PZ} is responsible for the lethal phenotype.

Precise excision of the insertion reverts the lethal phenotype.

Complements: simj01814. Complements: cpo01824. Complements: l(3)L0539L0539. Complements: simjj4A5.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (6)
Reported As
Name Synonyms
Secondary FlyBase IDs
    References (6)