Allele Dmel\cp309^s2172

General Information
Symbol	Dmel\cp309^s2172	Species	D. melanogaster
Name		FlyBase ID	FBal0028398
Feature type	allele	Associated gene	Dmel\cp309
Also Known As	l(3)s2172^s2172

Map ( GBrowse )	Untitled Document
Allele class
Mutagen	P-element activity
Recent Updates
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FB2013_03
FB2013_02
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Nature of the Allele
Allele class
Mutagen	P-element activity
Mutations Mapped to the Genome

Type Location Additional Notes References transposable element insertion site 3L:15,072,574..15,072,574 (BDGP Project Members, 1994-1999, Martinez-Campos et al., 2004, Spradling et al., 1999)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference
Caused by insertion	P{lacW}cp309^s2172 (BDGP Project Members, 1994-1999, Martinez-Campos et al., 2004, Spradling et al., 1999)
Cytology
Phenotypic Data
Phenotypic Class
	auditory perception defective (Martinez-Campos et al., 2004) lethal \| embryonic stage \| maternal effect \| poor (Perrimon et al., 1996) lethal \| larval stage \| recessive (Perrimon et al., 1996) lethal \| recessive (Spradling et al., 1999, Martinez-Campos et al., 2004) uncoordinated (Martinez-Campos et al., 2004) viable (with Df(3L)Brd15) (Martinez-Campos et al., 2004)
Phenotype Manifest In
	antennal sense organ & chordotonal organ (Martinez-Campos et al., 2004) centrosome & larval brain (Martinez-Campos et al., 2004) embryonic head & embryonic/first instar larval cuticle \| maternal effect (Perrimon et al., 1996) primary spermatocyte & centriole (Martinez-Campos et al., 2004) primary spermatocyte & spindle (Martinez-Campos et al., 2004)
Detailed Description
	Statement Reference Homozygous cp309^s2172 flies are viable, but exhibit a severe `uncoordinated' phenotype and die shortly after eclosion because they immediately get stuck in the food. The size and distribution of centrioles in wild-type and cp309^s2172 mutant interphase larval brain cells is indistinguishable. The same is true for testes cells. cp309^s2172 brain cells do not exhibit a higher mitotic index than control cells. Heterozygous cp309^s2172/+ flies have normal responses to a standard sound stimulus from electrodes placed in the antenna and head, while most (16/26) homozygous cp309^s2172 flies have no sound-evoked responses, and the amplitude of the response is also greatly reduced in those cp309^s2172 flies that do respond. In cp309^s2172/+ heterozygous antennae, the ciliary outer segments of the mechanosensory chordotonal neurons in the second antennal segment and of the chemosensory neurons in the third antennal segment are clearly visible. By contrast, in cp309^s2172 homozygous mutant antennae the sensory cilia in both the chordotonal organs and the olfactory bristles are absent or severely shortened and kinked, and the chordotonal cilia of the second segment fail to attach to the cuticle that connects the second and third antennal segments. In cp309^s2172 mutant testes, morphologically normal primary spermatocytes are formed that each contain two pairs of large, orthogonally arranged centrioles. However, as the spermatocytes mature, the centrioles often lose their orthogonal arrangement and partially fragment. cp309^s2172 mutant spermatocytes often form multipolar meiosis I spindles, with each spindle pole organised by at least one centriole. Although meiosis is highly abnormal in cp309^s2172 mutant spermatocytes, at least some cells develop into relatively normal looking sperm. However, the distribution of centrioles and nuclei in the cysts is often disorganised. Moreover, although the cp309^s2172 mutant sperm contain flagella, these are often nonmotile, even though electron microscopy reveals the sperm tails to be structurally normal. (Martinez-Campos et al., 2004) Germline clones produce eggs with patterning defects: heads are open. (Perrimon et al., 1996)
External Data
Linkouts
Interactions
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement Reference
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 1 )
Bloomington	12089 w¹¹¹⁸; P{lacW}cp309^s2172/TM3, Sb¹
Notes on Origin
Discoverer	M. Scott.
	Separable from: a lethal mutation on the chromosome. The original "l(3)s2172" chromosome is homozygous lethal, but is viable over a deficiency that removes cp309, indicating that the P{lacW}cp309^s2172 insertion in cp309 is viable and that there is a separate lethality on the chromosome. (Martinez-Campos et al., 2004)
Comments
	Complements: l(3)j2A2^j2A2. (BDGP Project Members, 1994-1999)
External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 4 )
Reported As
Symbol Synonym	cp309^s2172 d-plp²¹⁷² (Martinez-Campos et al., 2004) l(3)s2172 (Spradling et al., 1999) l(3)s2172^s2172 (Perrimon et al., 1996, BDGP Project Members, 1994-1999, )
Name Synonym
Secondary FlyBase IDs

References ( 5 )
Research paper	Martinez-Campos et al., 2004, J. Cell Biol. 165(5): 673--683 The Drosophila pericentrin-like protein is essential for cilia/flagella function, but appears to be dispensable for mitosis. [FBrf0179327] Spradling et al., 1999, Genetics 153(1): 135--177 The Berkeley Drosophila genome project gene disruption project. Single P-element insertions mutating 25% of vital Drosophila genes. [FBrf0111489] Perrimon et al., 1996, Genetics 144(4): 1681--1692 Zygotic lethal mutations with maternal effect phenotypes in Drosophila melanogaster. [FBrf0091142]
Personal communication to FlyBase	Meister and Braun, 1995.10, lacZ expression patterns for P{} insertions at Bloomington. lacZ expression patterns for P{} insertions at Bloomington. [FBrf0083714] BDGP Project Members, 1994-1999, BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. (Computer file) BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. (Computer file) [FBrf0067338]

Allele Dmel\cp309s2172

Allele Dmel\cp309^s2172