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General Information
Symbol
Dmel\gish04895
Species
D. melanogaster
Name
FlyBase ID
FBal0028445
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

Insertion in the second exon.

Insertion components
P{PZ}gish04895
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 1 )
Disease
Interaction
References
ameliorates  epilepsy
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

gish04895 homozygous adult males have small testes and along with the gish04895/gishMI08417 or gish04895/gishKG03891 transheterozygous mutants show decreased average number of germline stem cells in the testes already at post-eclosion day 1 and as the flies age the GSCs continue to be lost at a rapid rate, this however is not due to excessive apoptosis and neither do the mutant GSCs lose their adhesion to the hub cells as shown in gish04895/gishMI08417 males. No significant difference in the number of GSCs is observed in gish04895 heterozygotes, as compared to wild-type controls.

The germline stem cells in the heat-shock induced gish04895 mutant germline clones in testes of adult male flies are rapidly lost as the flies age.

gish04895/+ mutant flies exhibit a seizure-resistant phenotype, exhibiting a seizure threshold of about twice that of wild type. No other phenotypes are apparent; electrophysiology, behavior and morphology are all wild type.

Heterozygotes show a significant impairment in 30 minute and 3 hour memory after olfactory conditioning compared to wild-type flies, but do not show significant impairment in 3 minute memory.

Heterozygotes and homozygotes show normal shock and odour avoidance.

The nuclei of onion stage spermatids are scattered over the cyst in its basal third in homozygous males (the nuclei form a bundle at this stage in wild-type males). The actin cones also fail to form a bundle in the mutant spermatids. In late individualisation stage spermatids, the normal association between mitochondrial derivative and axoneme is lost in mutant males. In addition, although the major mitochondrial derivative has a horseshoe-shaped morphology characteristic of this stage, the minor mitochondrial derivative displays a morphology characteristic of the early individualisation stage.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressor of
Statement
Reference

gish[+]/gish04895 is a suppressor | partially of bang sensitive | semidominant phenotype of parabss1

NOT Suppressor of
Statement
Reference

gish[+]/gish04895 is a non-suppressor of bang sensitive phenotype of parabss1

gish[+]/gish04895 is a non-suppressor of bang sensitive phenotype of eas2

Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference

The bang sensitive phenotype seen in parabss1/+ mutant females can be partially suppressed with one copy of gish04895. Fewer flies exhibit bang-sensitivity and the threshold for seizure-like activity is partially rescued. A gish04895/+ background does not suppress the bang sensitive phenotypes in parabss1/parabss1 or parabss1/Y mutant flies.

The bang sensitive phenotype seen in eas2/+ mutant females is not suppressed by one copy of gish04895. The penetrance and threshold for seizure initiation are similar to in eas2 mutants alone.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments

The age-progressive loss of germline stem cells in adult male testes observed in gish04895 homozygotes as well as gish04895/gishKG03891 or gish04895/gishMI08417 transheterozygotes is fully rescued by combination with either gishgish.F. Similarly, combination with gishnos.F also rescues the phenotype in gish04895 homozygotes and both gish04895/gishKG03891 and gish04895/gishMI08417 mutants can be rescued by expression of gishScer\UAS.P\T.F under the control of Scer\GAL4nos.UTR.T:Hsim\VP16 (but not Scer\GAL4C587).

Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer

A. Spradling.

Comments
Comments

Complements Df(3R)sbd105, indicating that the insertion does not cause the male sterility, or has been mismapped.

Precise excision of the P-element completely suppresses the bang-sensitive phenotype and restores viability, but does not appear to revert the male sterility phenotype.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (7)
Reported As
Name Synonyms
Secondary FlyBase IDs
    References (11)