gish04895 homozygous adult males have small testes and along with the gish04895/gishMI08417 or gish04895/gishKG03891 transheterozygous mutants show decreased average number of germline stem cells in the testes already at post-eclosion day 1 and as the flies age the GSCs continue to be lost at a rapid rate, this however is not due to excessive apoptosis and neither do the mutant GSCs lose their adhesion to the hub cells as shown in gish04895/gishMI08417 males. No significant difference in the number of GSCs is observed in gish04895 heterozygotes, as compared to wild-type controls.
The germline stem cells in the heat-shock induced gish04895 mutant germline clones in testes of adult male flies are rapidly lost as the flies age.
gish04895/+ mutant flies exhibit a seizure-resistant phenotype, exhibiting a seizure threshold of about twice that of wild type. No other phenotypes are apparent; electrophysiology, behavior and morphology are all wild type.
Heterozygotes show a significant impairment in 30 minute and 3 hour memory after olfactory conditioning compared to wild-type flies, but do not show significant impairment in 3 minute memory.
Heterozygotes and homozygotes show normal shock and odour avoidance.
The nuclei of onion stage spermatids are scattered over the cyst in its basal third in homozygous males (the nuclei form a bundle at this stage in wild-type males). The actin cones also fail to form a bundle in the mutant spermatids. In late individualisation stage spermatids, the normal association between mitochondrial derivative and axoneme is lost in mutant males. In addition, although the major mitochondrial derivative has a horseshoe-shaped morphology characteristic of this stage, the minor mitochondrial derivative displays a morphology characteristic of the early individualisation stage.