Open Close
General Information
Symbol
Dmel\Hsp8308445
Species
D. melanogaster
Name
FlyBase ID
FBal0028548
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
scratch, Hsp9008445
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference
P{PZ} insertion into the intron, approximately 60bp from the junction of the first exon and the intron.
Insertion components
P{PZ}Hsp8308445
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 1 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference
Primary spermatocytes of Hsp8308445 mutants exhibit crystalline aggregates which are usually absent in wild-type testes.
77% of Hsp8308445/Hsp8308445, 67% of Hsp8308445/Hsp83e6D, 76% of Hsp8308445/Hsp83j5C2 and 57% of Hsp8308445/Hsp83e6A flies show a loss of locomotor behavioral rhythms under free-running conditions (after entrainment in light-dark cycles). 84% of homozygous flies show a loss of locomotor behavioral rhythms under free-running conditions (after entrainment in temperature cycles). Mutant flies show a reduced fraction of flies with rhythmic locomotor activity in light-dark cycles compared to control flies (88%-95% rhythmic); Hsp8308445/Hsp8308445 (57% rhythmic), Hsp8308445/Hsp83e6D (49% rhythmic), Hsp8308445/Hsp83j5C2 (47% rhythmic), Hsp8308445/Hsp83e6A (50% rhythmic). Homozygous flies show a reduced fraction of flies with rhythmic locomotor activity (74% in temperature cycles compared to control flies (97% rhythmic). Hsp8308445/Hsp8308445, Hsp8308445/Hsp83e6D, Hsp8308445/Hsp83j5C2 and Hsp8308445/Hsp83e6A mutants show highly variable locomotor activity patterns, including fully rhythmic, arrhythmic, and complex behavioral profiles, in contrast to wild-type flies, which show little variation among individuals.
Hsp83e6D/Hsp8308445 females are reasonably healthy and produce large numbers of eggs. Very few of the embryos derived from these eggs cellularise. The 1-2% of the embryos that eventually secrete cuticle have on average 3 abdominal segments rather than the normal 8.
Hsp8308445 males have crystals in the spermatocytes in the presence of the Ste+ allele.
Mutant females show increased mortality after 12 hours of sleep deprivation. This lethality is not prevented by pre-heating the flies before sleep deprivation. Heterozygotes and homozygotes show a sleep rebound (homeostatic response) corresponding to fivefold that of wild-type flies in response to 12 hours of sleep deprivation.
Homozygous and Hsp8308445/Hsp83P582 males show defects during spermatogenesis. The number and shape of spermatocytes within 16-cell cysts are mostly normal (5-10% are abnormal). Spermatids with variable number, size and shape of nuclei and nebenkern are seen. Needle-shaped crystals are present throughout developing spermatocytes and spermatids. Individualised sperm are present but they are not motile and are fragile. Bundles containing aberrant axonemes are seen in homozygous testes and variable numbers of mitochondrial derivatives (1-10) are seen. The packing and spacing of the spermatid bundles is disrupted. The membranes of the axoneme are often incomplete. Hsp8308445/Hsp83e3A males show defects during spermatogenesis. The number and shape of spermatocytes within 16-cell cysts are mostly normal (5-10% are abnormal). Spermatids with variable number, size and shape of nuclei and nebenkern are seen. Crystal structures are seen in some developmental stages. Individualised sperm are present but they are not motile and are fragile. Hsp8308445/Hsp83e6D and Hsp8308445/Hsp83e6A males show defects during spermatogenesis. Excessive numbers of primary spermatocytes are seen in many developing cysts. Spermatids with variable number, size and shape of nuclei and nebenkern are seen. Needle-shaped crystals are present throughout developing spermatocytes and spermatids. Individualised sperm are present but they are not motile and are fragile. Hsp8308445/Hsp83e4A males show defects during spermatogenesis. Excessive numbers of primary spermatocytes are seen in many developing cysts. Spermatids with variable number, size and shape of nuclei and nebenkern are seen. Individualised sperm are present but they are not motile and are fragile.
Needle shaped crystals accumulate through developing germline. Spermatids contain nuclei and nebenkern of variable size. Phenotype resembles that of X0 males.
Needle-shaped crystals accumulate throughout developing male germ line. Spermatids contain nuclei and Nebenkerne of variable size. Phenotype resembles that of X0 males.
External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Suppressor of
Statement
Reference
Hsp8308445/Hsp83[+] is a suppressor of arista | supernumerary phenotype of obk1
Additional Comments
Genetic Interactions
Statement
Reference
The mechanical stress sensitivity of Tpisgk-1 animals is significantly suppressed if they are also mutant for Hsp8308445.
Hsp8308445 males have needle-shaped crystals in the spermatocytes in the presence of the Steunspecified allele.
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Hsp8308445 complements Hsp83e6D, Hsp83e3A, Hsp83e6A, Hsp83e4A and Hsp83P582 for viability but not for fertility. Hsp8308445 complements Hsp83P582 for viability and female fertility but not for male fertility.
Not sterile over Df(3L)HR370 or Df(3L)M21.
Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
A. Spradling.
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (12)
Reported As
Name Synonyms
Secondary FlyBase IDs
    References (18)