General Information
Symbol
Dmel\w+mC
Species
D. melanogaster
Name
FlyBase ID
FBal0028610
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
Act5C-Gal4, actin-GAL4, act-GAL4, Actin5c-GAL4, Hml-GAL4, per-Gal4, UAS-GMA, vgBE-lacZ, P{lacW}, vgBE-lacZ
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference
4.15kb SpeI fragment of the w gene that carries a deletion of a large part of the first intron and the regulatory region is reduced to 300bp preceding the transcription start site.
Construct: A 'mini-white' gene constructed by deletion of the HindIII-XbaI fragment from the long 5' intron. Carried by the Casper series of vectors.
Allele components
Product class / Tool use(s)
Encoded product / tool
Associated Sequence Features
Carried on aberration
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Model Data
Disease Ontology
Models ( 0 )
Disease
Evidence
References
Interactions ( 0 )
Disease
Interaction
References
Comments ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference
Males carrying w+mC in a w[+] background show comparable levels of courtship discrimination as Oregon-R males (showing higher levels of courtship towards decapitated females than towards decapitated males). groups of naive males carrying w+mC in a w[+] background do not show chaining behaviour under daylight conditions, the same behaviour as is seen in Oregon-R males.
Uninsulated w+mC insertions (in a w[-] background) exhibit a wide range of eye colours, depending on the site of transgene insertion.
piwi, aub and spn-E are suppressors of repeat-induced silencing, as seen at heterochromatic tandem arrays of insertions of the P{lacW} transposon.
Eye colour: approximately 10% of P{GMroX1} transgenic stocks show sex-specific eye pigmentation patterns; in many cases mini-w expression is very low or silent in females but in the same lines, males express mini-w, often in mosaic sectors. The addition of a Y chromosome does not overcome the silencing seen in the females. Several of the P{GMroX1} lines show pairing-sensitive repression; showing significantly less eye pigmentation when homozygous than when hemizygous.
Eye colour: Expression of w+mC can restore pigment levels in the eyes of w1118 flies. No pairing-sensitive repression is seen; flies carrying two copies of w+mC have darker eyes than than heterozygotes carrying one copy of w+mC.
Transfection of the w+mC as part of the P{Ubi-GFP.D} construct, followed by heat shock, leads to male mutants that show an increase in male-male courtship compared to wild-type flies.
Eye colour: flies carrying w+mC in a w1118 background have eye colours ranging from yellow to red, depending on the P{CaSpeR-3} insertion strain used.
Heat induced expression of Scer\GAL4hs.PB in P{UAS-lacZ.U/l5} flies does not affect eye pigment levels.
Light affects basal male courtship: males are inhibited by white light
Eyes are light yellow.
Male-male courtship occurs between flies following ectopic expression of w+mC. Male-male courtship behaviour increases with age, flies 1 week old or older show high levels of courtship compared to one day posteclosion males. Olfactory, visual and gustatory responses are not affected by heat shock. Surgical ablation of the antenna and/or maxillary palps, or the wings has no effect on male-male courtship. Under red light male-male courtship is substantially reduced, restoration of white light increases courtship. For all classes of surgically treated flies red light diminishes courting activity. Male-male courtship is not due simply to an absence of rejection behaviour (wing flicking) as heat shocked males will court a non-w+mC male exhibiting rejection behaviour. Also the courting is not due to increased sexual attractance as non-w+mC males will not court heat shocked w+mC males.
Transformants carrying one copy of w+mC have more lightly pigmented eyes than flies carrying two copies.
Partially restores pigmentation in w- eyes. Heat shock during a period late in development of flies also carrying P{GAL4::Pc-Hsp70} exhibit a uniform reduction or abolition of eye pigmentation. Heat induction earlier in development does not affect eye pigmentation.
Fully dosage compensated on the X chromosome but only partly dosage compensated on the autosomes. Around 50% of X linked inserts display hyper dosage compensation, also observed for wsp1 and wa.
The w+mC mutation shows incomplete dosage compensation when inserted in the second or third chromosome (comparison of heterozygous males and homozygous females). The w+mC mutation of P{yp1,2,mini-w} or P{otu-lacZ.R} insertions into the X chromosome shows complete or hyperdosage compensation, such that hemizygous males have either equal or greater level of pigmentation than females.
Transformant heat shocked males exhibit homosexual courtship behaviour.
Variegated expression of w occurs when the minigene is present in repeat arrays. Variegation is particularly strong for repeated transposons at a euchromatic site near heterochromatin, but also results from repeats at a site distant from heterochromatin. Inactivation strengthens with increasing copy number. The variegated phenotypes are subject to the effects of genetic modifiers of PEV.
Degree of pigmentation varies from very pale yellow to dark red, depending on genomic site of insertion.
When introduced into w- flies the eye colour can vary from pale yellow to red depending on site of integration.
External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement
Reference
w+mC has eye color defective phenotype, enhanceable by e(y)11
Suppressor of
Statement
Reference
w+mC is a suppressor of eye color defective phenotype of g50e
Phenotype Manifest In
Enhanced by
Statement
Reference
w+mC has pigment cell phenotype, enhanceable by e(y)11
w+mC has phenotype, enhanceable by Wowγb
w+mC has phenotype, enhanceable by Wowγe
w+mC has phenotype, enhanceable by Wowems1
w+mC has phenotype, enhanceable by Wowhd1
NOT Enhanced by
Statement
Reference
w+mC has phenotype, non-enhanceable by Low1
NOT suppressed by
Statement
Reference
w+mC has phenotype, non-suppressible by Low1
Suppressor of
Statement
Reference
w+mC is a suppressor of pigment cell phenotype of g50e
Additional Comments
Genetic Interactions
Statement
Reference
Transfection of w+mC is capable of increasing the amount of pigment in the eyes of we(g), g2/Y mutant flies, depending on the level of its expression. Strong expression of w+mC is capable of restoring pigment to g2/Y single mutant levels, thus rescuing the we(g) effect. One copy of the w+mC transgene is sufficient to increase the amount of red pigment in the eyes of both male and female w+, g50e flies.
The eye pigmentation of flies carrying w+mC in a w1118 flies is reduced by e(y)11 in a number of different P{CaSpeR-3} insertion strains.
The eye colour of flies carrying w+mC on the X chromosome is unaffected by a single copy of Low1.
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Rescues
Partially rescues
w+mC partially rescues w1118
w+mC partially rescues w1
Comments
w+mC rescues the low courtship vigor index seen in daylight in w1118 males in response to a receptive virgin female. w1118 males rescued with w+mC court decapitated virgin females vigorously, independent of light conditions. The reduced sexual discrimination of w1118 males in the dark (they show similar courtship levels towards decapitated males and decapitated females) is not rescued by w+mC. In daylight, w1118 males rescued by w+mC show a clear courtship preference for decapitated females over decapitated males, although the level of courtship towards decapitated males shown by the rescued males is higher than the level seen in Oregon-R males under these conditions. Groups of naive w1118 males rescued by w+mC show chaining behaviour under daylight conditions. This behaviour can be rescued by Dp(1;Y)w+y+.
One copy of w+mC restores courtship disinhibition after repeated ethanol exposure (observed as intermale courtship) in w1118 males.
Transfection of w+mC into w1 mutant flies increases pigment levels in the eye from <1% to between 21 and 97%, depending on the insertion of the w+mC construct.
Images (0)
Mutant
Wild-type
Stocks (10,105)
Notes on Origin
Discoverer
Comments
Comments
Transgene expresses subnormal amounts of eye pigment.
Used as a reporter in experiments with a chromatin insulator from the chicken Β-globin domain.
Carried in cosmid vector cosP{cosPer}.
129 different lines carrying P{CaSpeR} have been generated by transformation, in order to investigate the effect of location of the P{CaSpeR} element, gene dosage and sex of the transformed flies on w+mC expression. lzb suppresses the expression of w+mC, the degree of suppression depending on the location and dosage of w+mC.
Shows greater (full) dosage compensation in X chromosomal than in autosomal sites.
The w+mC allele has been used as a semidominant marker in the introgression of marked segments of D.mauritiana chromosomes into D.simulans.
Carried in plasmids "P{hs-per}" and "P{hs-tim-GFP}".
Ufo1 suppresses w eye phenotype.
External Crossreferences and Linkouts ( 10 )
Crossreferences
GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
Synonyms and Secondary IDs (4)
Reported As
Symbol Synonym
w+mC
wR4
Name Synonyms
Secondary FlyBase IDs
  • FBal0046767
References (2,588)