Allele Dmel\w+mW.hs
| General Information | |||
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| Symbol | Dmel\w+mW.hs | Species | D. melanogaster |
| Name | FlyBase ID | FBal0028611 | |
| Feature type | allele | Associated gene | Dmel\w |
| Also Known As | whs | ||
| Allele class | |||
| Mutagen | in vitro construct - minigene, in vitro construct - deletion, in vitro construct - regulatory fusion | ||
Recent Updates
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| Description |
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| FB2013_03 | |||
| FB2013_02 | |||
| All updates | Click here to see a list of all updates to this record from FB2010_08 and on. | ||
Nature of the Allele
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| Allele class | |||
| Mutagen | |||
| Mutations Mapped to the Genome | |||
Type Location Additional Notes References | |||
| Associated Sequence Data | |||
| DDBJ
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EMBL / GenBank | DNA sequence Protein sequence Name | ||
| UniProtKB/Swiss-Prot | |||
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| Progenitor genotype | |||
| Nature of the lesion | Statement Reference A mini-w gene is expressed under the control of an Hsp70 promoter. w gene expression is under the influence of FLIP recombinase. Expression of a fragment of w genomic DNA with a 2.2kb deletion of 3' flanking sequences and a reduction in the size of the largest intron to 356bp is driven by a 388bp fragment of an Hsp70 gene. Construct: A 'mini-white' gene constructed by deletion of the XbaI-HindII fragment from the long 5' intron. Carried by the W6 and W8 series of vectors. | ||
| Carried in construct | |||
| Carried on aberration | |||
| Cytology | |||
Phenotypic Data
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Phenotypic Class
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Phenotype Manifest In
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Detailed Description
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Statement Reference Eye colour: red even in the absence of heatshock. All flies carry the w1118 mutation, all observed pigmentation is due to expression of the w transgene. P{hsFLP} P{FRT(whs)} heat induced expression during third instar causes mosaic pattern in the anterior and posterior adult eye. A stripe exhibiting little loss of w pigmentation lies slightly anterior to the morphogenetic furrow, this is due to these cells exhibiting a slightly feeble response to heat shock (as seen from P{hsp70-lacZ} expression pattern). P{FRT(whs)} P{hsFLP} bearing flies heat shocked 6 hours after puparium formation exhibit the same level and pattern of pigmentation as individuals not heat shocked. w+mW.hs transcription at this stage is without phenotypic consequence. The coding region of w+mW.hs located in the chromosome, P{FRT(whs)}, or extrachromosomally can respond to the mechanism of dosage compensation, expressed more strongly in males than in females. Some lines of P{FRT(whs)} exhibit position effect variegation; enhanced in XO males and suppressed by Su(var)205502. P{hsFLP} and P{70FLP} induced excision 6 hours after puparium formation alleviates the variegating position effects. Lines carrying P{FRT(whs)} near the heterochromatin require daily heat shocks from 18 hours after puparium formation for 5-6 days to give the most reversion. P{70FLP} mediated excision of some heterochromatic insertion lines 6-30 hours after egg laying gives rise to entirely white-eyed adults, other lines exhibit a reduction in the rate of excision so show areas of pigment. Induction during third larval instar causes loss in the anterior portion of the eye. Transformant heat shocked males exhibit homosexual courtship behaviour. Wild type eye pigmentation. The eye pigmentation of Df(1)w67c2 homozygotes carrying w+mW.hs is reduced if the flies are also carrying two copies of LTSV\RBZhs.w and are heat shocked for 1 hour at 37oC every 12 hours throughout development. Eye colour: red, as homozygote. Eye colour: orange eyes that darken and become slightly mottled with age, as heterozygote. | |||
External Data
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Interactions
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Phenotypic Class
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Other | |||
Statement Reference | |||
Phenotype Manifest In
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Suppressed by | |||
Statement Reference w+mW.hs has phenotype, suppressible by Su(var)205502 | |||
Other | |||
Statement Reference | |||
Additional Comments
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Genetic Interactions
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Statement Reference The progeny of male flies homozygous for w+mW.hs (inserted into centric heterochromatin of the second chromosome-39C4) mated with AGO2414 homozygous females have different eye colour, but a relatively uniform pigmentation across the eye, with little evidence of variegation, indicating heterochromatic silencing. | |||
Xenogenetic Interactions
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Statement Reference | |||
Complementation & Rescue Data
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| Comments | |||
Stocks
( 6999 ) | |||
| Bloomington | 5076 33807 4921 6323 6927 7148 1774 1782 1874 2683 2703 | ||
| Kyoto | 108022 | ||
Notes on Origin
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| Discoverer | |||
Comments
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Carried in episome eFRT-w.G. The w gene is turned on in the P{FRT(whs)} construct and Scer\FLP1-mediated expression produces a functional extrachromosomal w gene that can be expressed in non-dividing cells but is lost in dividing cells. Carried in episome eFRT-w.G. The presence of Su(var)2051, Su(var)2052 or additional heterochromatin provided by an attached X suppress position effect variegation (PEV) of lines with insertions in centromeric regions or in the fourth chromosome. Insertions near the telomeres 2L, 2R or 3R show no suppression. Variegating transgenes show a reduction in accessibility to restriction enzyme digestion. This suggests that the chromatin packaging of an insert showing PEV is altered. Pericentric transgenes are packaged in a more regular array than euchromatic transgenes. | |||
External Crossreferences & Linkouts
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| Other Crossreferences | |||
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Synonyms & Secondary IDs
( 4 ) | |||
| Reported As | |||
| Symbol Synonym | hsp70-white w+mW.hs w+mW | ||
| Name Synonym | |||
| Secondary FlyBase IDs | |||
References
( 340 ) | |||
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Recent research papers ( 1 ) | |||
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Recent reviews (0)
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| All reviews listed in FlyBase were published before 2011 | |||
Recent Updates
External Crossreferences & Linkouts