Deletion of the 5' end of W.
W05014/WrvX1 pupal eye discs lack any evidence of "early stage" cell death (this normally occurs between 18-24 hours after puparium formation). The ommatidia in these pupal retinas are disorganised compared to wild type due to the excess of cells present. The retinas often appear to be attached to what seems to be the antennal disc in the pupae.
WrvX1/Df(3L)H99 mutant animals exhibit ectopic branching on their aristae. The antennae of these animals are larger than wild-type, the aristae have a hairy appearance. While the number and position of anterior branches remain similar to wild-type, the posterior aristae show fewer long lateral branches along the dorsal posterior axis. These extra branches are longer than typical dorsal branches yet shorter than anterior and posterior branches. In addition, the ectopic branches are located very close to each other and often stemmed from the same, or an adjacent position on the central core, unlike the regular spacing seen in wild-type. Also mutant aristae do not terminate in the typical forked pattern but split into 4-5 small branches arranged in a spiral fashion about the central axis.
WrvX1 mutant embryos have increased numbers of midline glial cells at stage 17.
The ventral crustacean cardioactive peptide immunoreactive (vCCAP-IR) neurons of the ventral nervous system die on schedule in heterozygous flies.
Homozygotes display significantly reduced viability. Pronounced defect in the morphogenetic movements of head involution which results, in part, from a failure of the dorsal fold to migrate anteriorly. Otherwise the segmented cuticle is normal and individuals reach advanced stages. There is less apoptosis when compared to wild type, most noticeably in the head region prior to completion of head involution.
hidrvX1/Df(3L)H99 is a suppressor | partially of increased cell death | third instar larval stage phenotype of Scer\GAL4GMR.PU, p53GUS.PB
hidrvX1/W[+] is a suppressor of increased cell death phenotype of Scer\GAL4GMR.PF, rprUTR.UAS.Tag:HA
hidrvX1/Df(3L)H99 is a suppressor of increased cell death | embryonic stage phenotype of bcdunspecified
hidrvX1 has midline glial cell phenotype, enhanceable by rlSem/rlSem
hidrvX1/W[+] is a suppressor of eye phenotype of Scer\GAL4GMR.PF, rprUTR.UAS.Tag:HA
hidrvX1/hidrvX1 is a suppressor of midline glial cell phenotype of EgfrDN.UAS, Scer\GAL4sli.PS
hidrvX1/hidrvX1 is a suppressor of midline glial cell phenotype of spi1
hidrvX1/Df(3L)H99 is a non-suppressor of eye disc | posterior | third instar larval stage phenotype of Scer\GAL4GMR.PU, p53GUS.PB
hidrvX1/Df(3L)H99 is a non-suppressor of larval head phenotype of bcdunspecified
hidrvX1/Df(3L)H99 is a non-suppressor of larval thorax phenotype of bcdunspecified
hidrvX1/Df(3L)H99, bcdunspecified has larval abdominal segment phenotype
A WrvX1/Df(3L)H99 background significantly suppresses the cell death seen when p53GUS.PB is expressed in third instar eye discs under the control of Scer\GAL4GMR.PU.
A WrvX1 heterozygous background suppresses the degree of rprUTR.Scer\UAS.T:Ivir\HA1-induced eye ablation.
The increased cell death that occurs in the presumptive head and thorax regions of bcdunspecified embryos can be suppressed by mutation of W, as observed in bcdunspecified, Df(3L)H99/WrvX1 double mutants. bcdunspecified, Df(3L)H99/WrvX1 double mutant larvae have an expanded first abdominal segment that is transformed into a posterior A8 identity, but still lack the head and thorax. Therefore, the transformed abdominal segment must usually undergo cell death in bcdunspecified single mutants, due to the mis-specificity of the cells within it.
The increase in midline glial cells seen in WrvX1 mutant embryos is slightly enhanced by rlSem/rlSem (from 5.8 (n=182) to 6.6 (n=91)). The loss of midline glial (MG) cells in EgfrDN.Scer\UAS; Scer\GAL4sli.PS embryos is suppressed by WrvX1/WrvX1. Approximately twice as many MG cells survive as in wild-type (average of 6.1 per segment n=201, compared to average = 2.8 for wild-type). The loss of midline glial (MG) cells in spi1 homozygous embryos is suppressed by WrvX1/WrvX1. Approximately twice as many MG cells survive as in wild-type (average of 5.7 per segment n=133, compared to average = 2.8 for wild-type).