Expression of kuzScer\UAS.cFa in a Appld/+; Scer\GAL4Mef2.247.Switch heterozygous background exacerbates the short term memory deficit found in this background. These flies exhibit this exacerbated defect regardless of whether the flies are fed with RU for 48 hours before conditioning. Appld/+; Scer\GAL4Mef2.247.Switch/kuzScer\UAS.cFa flies fed with RU and testing immediately after conditioning exhibit wild-type learning. The ability to avoid electric shocks as well as their olfactory acuity to each odour after electric shock exposure is unaffected.
Adult specific expression of BaceScer\UAS.cCSa driven by Scer\GAL4Mef2.247.Switch (activated in adult flies fed RU486) significantly enhances short term memory defects in an olfactory aversive conditioning paradigm compared to Appld/+ flies or to controls not fed RU486; there are no significant effects on learning, olfactory acuity or shock reactivity.
Adult specific expression of BaceScer\UAS.cCSa driven by Scer\GAL4c739 (using the TARGET system) significantly enhances short term memory defects in an olfactory aversive conditioning paradigm compared to Appld/+ flies or to genetic or temperature controls; there are no significant effects on learning, olfactory acuity or shock reactivity.
Expression of ApplScer\UAS.cCSa under the control of Scer\GAL4Appl.G1a partially suppresses the vacuolisation seen in 4 day old Appld SNF4Aγloe double mutant males, returning the phenotype to the level seen in SNF4Aγloe single mutants.
The loss of anterior scutellar macrochaetae that is seen in Appld flies is partially suppressed by APP-BP1Ex62/+, but is not suppressed by APP-BP1Ex183/+. The loss of posterior scutellar macrochaetae that is seen in Appld flies is not suppressed by APP-BP1Ex62/+ or APP-BP1Ex183/+.
Overexpression of Fas2Scer\UAS.cAa in both presynaptic and postsynaptic cells (under the control of Scer\GAL4C164 and Scer\GAL4C57) in Appld mutants leads to a wild-type number of boutons, in contrast to the two-fold increase when Appl levels are normal.
Expression of hepCA.Scer\UAS, under the control of Scer\GAL4P2.4.Pdf, in an Appld mutant background still induces the significant axonal extension seen when the transgene is expressed in a wild-type background.
Expression of Applsd.Scer\UAS in RU-fed flies suppresses the short-term memory defect found in Appld/+; Scer\GAL4Mef2.247.Switch flies. In contrast, in the absence of RU-feeding, Appld/+; Scer\GAL4Mef2.247.Switch/Applsd.Scer\UAS flies exhibit scores that are indistinguishable from controls and significantly lower than that found in wild-type flies.