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General Information
Symbol
Dmel\CycAhs.PK
Species
D. melanogaster
Name
heat shock construct of Knoblich
FlyBase ID
FBal0029120
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
hs-cycA, HS-cyclin A
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference
NcoI-NheI fragment of CycA cDNA is driven from an Hsp70 promoter.
Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference
Overexpression of CycAhs.PK results in an extra mitotic division in 4% of egg chambers.
Unlike wild-type, CycAC8LR1 mutants do not show an increased proportion of metaphase figures after exposure to radiation.
When CycAhs.PK is over-expressed in adult females germline cysts with an aberrant number of cells are produced. Five days after heat-shock treatment cysts containing 32 instead of 16 cells are seen in 3% of egg chambers. The oocyte contains 5 ring canals (as compared to 4 in wild-type). Very rarely, cysts with an intermediate number of cells (in between 16 and 32) are seen.
Heat induced expression during third larval instar results in a rough eye phenotype, this correlates with the induction of many cells into S phase in eye discs. Wild type Rca1 cannot suppress entry into S phase.
Expression of CycAhs.PK in embryos using a 30 minute heat shock induces premature S phase in epidermal cells and endocycling tissues. Expression of CycAhs.PK using a 10 minute heat shock does not induce S phase in epidermal cells of wild-type embryos carrying a single copy of CycAhs.PK.
Ectopic S phase entry in the morphogenetic furrow. ruxhs.PT inhibits the ectopic S phase entry at the morphogenetic furrow but not in anterior or posterior cells.
Heat induced expression induces entry into S phase in cells of the amnioserosa.
External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference
Many cells of heatshocked embryos carrying cdc2AF.hs and CycAhs.PK fail to delay mitosis after irradiation.
Simultaneous over-expression of CycAhs.PK and cdc2AF.hs is sufficient to induce mitosis throughout the entire embryo. This phenotype is suppressed by the addition of ruxhs.PT.
A single copy of CycAhs.PK is sufficient to cause homozygous lethality in grp06034 embryos, even without heatshock.
Heat induced coexpression of CycAhs.PK and Cdk1AF.hs induces proliferation in quiescent pole cells. Ectopic mitoses are followed by cell division in pole cells. Heat induced coexpression of CycAhs.PK and Cdk1hs.PS fails to induce proliferation in quiescent pole cells.
Expression of CycAhs.PK using a 10 minute heat shock induces S phase in epidermal cells of ruxunspecified embryos carrying a single copy of CycAhs.PK. S phase is widely induced in the internal tissues that endocycle in wild-type embryos in CycEAR95 embryos carrying two copies of CycAhs.PK, within 1 hour of heat shock. Induction of S phase is also seen in epidermal cells, although not until 180 minutes after heat shock. The efficiency of S phase induction in embryos is dramatically increased if CycAhs.PK is co-expressed with cdc2AF.hs using heat shock.
CycAhs.PK flies exhibit ectopic S phase entry in the morphogenetic furrow, ruxhs.PT inhibits the ectopic S phase entry at the morphogenetic furrow but not in anterior or posterior cells.
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
CycAhs.PK
Name Synonyms
heat shock construct of Knoblich
Secondary FlyBase IDs
    References (19)