Allele Dmel\Dl^RevF10

General Information
Symbol	Dmel\Dl^RevF10	Species	D. melanogaster
Name		FlyBase ID	FBal0029366
Feature type	allele	Associated gene	Dmel\Dl
Also Known As	Dl^rev10, Delta^revF10, Dl^rev10e

Allele class	loss of function allele, amorphic allele - genetic evidence
Mutagen
Recent Updates
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FB2013_03
FB2013_02
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Nature of the Allele
Allele class	amorphic allele - genetic evidence (Heitzler and Simpson, 1991, Kunisch et al., 1994, Tomlinson and Struhl, 1999, Sun and Deng, 2005) loss of function allele (Doherty et al., 1996, Micchelli et al., 1997, Seugnet et al., 1997, Dominguez et al., 2004, Kamimura et al., 2004, Kamimura et al., 2004)
Mutagen
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype	Dl^j8C3 (Haenlin, 1998.9.18)
Nature of the lesion	Statement Reference Excision of the promoter region, transcription start site and first exon. (Heitzler and Simpson, 1991) Revertant of a P-element insertion located between -20 and -30 upstream of the Dl transcription start site. Deletion of approximately 760bp removing most of the first exon and the proximal part of the promoter (including the TATA box). (Haenlin et al., 1990)
Cytology
Phenotypic Data
Phenotypic Class
	mitotic cell cycle defective \| somatic clone (Baonza and Freeman, 2005) planar polarity defective \| somatic clone (Tomlinson and Struhl, 1999, Fanto and Mlodzik, 1999) visible \| dominant (Kamimura et al., 2004) visible \| recessive (Zeng et al., 1998) visible \| somatic clone (Dansereau et al., 2002)
Phenotype Manifest In
	adult midgut (Ohlstein and Spradling, 2007) anterior scutellar bristle (Kamimura et al., 2004) chemosensory ventral triple row bristle (Doherty et al., 1996) dMP2 neuron \| ectopic \| germline clone (Rusconi and Corbin, 1999) dorsal mesothoracic disc & sensory mother cell \| dorsal/ventral compartment boundary (Pavlopoulos et al., 2001) dorsal triple row (Doherty et al., 1996) embryonic/larval central nervous system (Seugnet et al., 1997) embryonic/larval central nervous system \| germline clone (Seugnet et al., 1997) embryonic/larval dorsal vessel \| precursor (Jagla et al., 1997) embryonic/larval peripheral nervous system (Seugnet et al., 1997) embryonic/larval peripheral nervous system \| germline clone (Seugnet et al., 1997) eye disc & neuron (Ligoxygakis et al., 1998) eye disc \| somatic clone (Baonza and Freeman, 2005) eye photoreceptor cell \| somatic clone (Tomlinson and Struhl, 1999, Overstreet et al., 2004, Fanto and Mlodzik, 1999) female germline stem cell \| germline clone (Ward et al., 2006) follicle cell \| cell non-autonomous \| germline clone (Deng et al., 2001) follicle cell \| somatic clone (Poulton et al., 2011) joint (Rauskolb and Irvine, 1999) larval outer optic anlage \| somatic clone (Wang et al., 2011) macrochaeta \| ectopic (Zeng et al., 1998) mechanosensory ventral triple row bristle (Doherty et al., 1996) medial triple row (Doherty et al., 1996) morphogenetic furrow \| somatic clone (Baonza and Freeman, 2001) neuroblast NB5-2 (Seugnet et al., 1997) neuroblast NB5-2 \| germline clone (Seugnet et al., 1997) neuroblast NB7-4 (Seugnet et al., 1997) neuroblast NB7-4 \| germline clone (Seugnet et al., 1997) ommatidium \| somatic clone (Tomlinson and Struhl, 2001, Tomlinson and Struhl, 1999, Fanto and Mlodzik, 1999) photoreceptor cell R2 \| somatic clone (Tomlinson and Struhl, 2001) photoreceptor cell R3 (Fanto and Mlodzik, 1999) photoreceptor cell R4 \| somatic clone (Fanto and Mlodzik, 1999) photoreceptor cell R5 \| somatic clone (Tomlinson and Struhl, 2001) photoreceptor cell R8 (Ligoxygakis et al., 1998) photoreceptor cell R8 \| somatic clone (Tomlinson and Struhl, 2001, Overstreet et al., 2004) posterior scutellar bristle (Kamimura et al., 2004) scutum & macrochaeta \| somatic clone (Zeng et al., 1998) segment \| germline clone (Seugnet et al., 1997) segment border muscle \| precursor (Jagla et al., 1998) sensory mother cell & mesothoracic tergum & third instar larva \| ectopic \| somatic clone (Pitsouli and Delidakis, 2005) trichome of the posterior wing margin (Doherty et al., 1996) ventral triple row (Doherty et al., 1996) vMP2 neuron \| ectopic \| germline clone (Rusconi and Corbin, 1999) wing \| somatic clone (Zeng et al., 1998) wing vein (Doherty et al., 1996) wing vein \| somatic clone (Dansereau et al., 2002, Zeng et al., 1998) wing vein L3 (Kamimura et al., 2004)
Detailed Description
	Statement Reference Clones of homozygous Dl[RevF10] mutant ovarian follicle cells tend to have larger nuclei than surrounding wild-type cells. (Poulton et al., 2011) Homozygous clones in the outer proliferation center neuroepithelium (induced at late-first or early-second instar and analysed at late-third instar) adopt rounded or irregular cell morphology rather than the normal columnar epithelial cell morphology in 87.5% of cases. (Wang et al., 2011) Homozygous intestinal stem cell (ISC) clones in the adult midgut often grow into tumours, or into a tumour plus a single enterocyte (EC). Other homozygous ISC clones differentiate into a single EC. (Ohlstein and Spradling, 2007) Dl^RevF10 mutant germline stem cells differentiate and leave the niche, resulting in an empty germarium phenotype when both germline stem cells are mutant. (Ward et al., 2006) Clones of Dl[RevF10] mutant cells in a Minute/+ background in the position of the second mitotic wave of the eye disc do not enter S phase and do not undergo mitosis. However, occasional cells that incorporate BrdU (a marker of S phase) are observed in some Dl[RevF10] clones. Also, there is a rescue of the G1 block at the borders of the Dl[RevF10] clones. (Baonza and Freeman, 2005) Dl^RevF10 third instar nota clones exhibit at least eight sensory organ precursors (SOPs) in approximately 11% of SOP positions scored. Approximately 11% of SOP positions display between four and eight ectopic SOPs, while approximately 41% exhibit between one and four SOPs, with 37% exhibiting one SOP, as in wild-type. (Pitsouli and Delidakis, 2005) Female flies heterozygous for Dl^RevF10 show thickened wing vein L3 and duplicated anterior and posterior scutellar macrochaetae. (Kamimura et al., 2004) Photoreceptor cells are absent from the centers but not the borders of Dl^RevF10 homozygous somatic clones in the eye disc. R8 photoreceptor cells cluster just inside the boundaries of these clones. (Overstreet et al., 2004) Heterozygotes show no effect on the notum macrochaetae. (Escudero et al., 2003) Large clones in the eye disc that cross the morphogenetic furrow cause its anterior displacement. (Baonza and Freeman, 2001) No extra cell divisions are seen in homozygous follicle cell clones. In egg chambers containing homozygous germline clones, defects are seen in cell cycle regulation in follicle cells surrounding the germline clones. The follicle cells retain their normal apical-basal polarity in stage 8-9 egg chambers (some epithelial defects are seen in later egg chambers). (Deng et al., 2001) Sensory organ precursors (SOPs) at the prospective wing margin of third instar larval wing discs are lost in Dl^RevF10 somatic clones, except in cases where a cell within the clone abuts wild-type wing margin cells. There may be some non-autonomous effect of these clones on SOPs: 83% of the clones have wild-type (WT) SOPs next to Dl^RevF10 non-SOPs versus 14% with Dl^RevF10 SOPs next to WT non-SOPs, and almost no pairs of adjacent WT and Dl^RevF10 mutant SOPs (3%). (Pavlopoulos et al., 2001) Somatic clones in the eye lead to defects. Ommatidia that are entirely Dl^RevF10 are grossly abnormal, as are the majority of mosaic ommatidia in which many of the cells are Dl^RevF10. However the remaining mosaic ommatidia develop a normal complement of photoreceptors. Notably almost none of these ommatidia contain mutant R8 cells and only a few contain mutant R2 or R5 cells. No ommatidia are seen that are mutant for both R1 and R6 cells. All other possible mosaic combinations of the R1/6/7 trio occur at frequencies similar to that in wild-type control clones. (Tomlinson and Struhl, 2001) Clones in the eye (located away from the dorsoventral midline) result in an excess of photoreceptors in the middle of the clone. At the margins of the clone, in ommatidia that are mosaic in the R3/R4 photoreceptors, the Dl⁺ cell always adopts the R3 fate. This can result in polarity reversals and inverted chirality. Ommatidia containing the normal number of photoreceptor cells in which the R3/R4 pair are mutant for Dl^RevF10 show an R3/R3 phenotype. (Fanto and Mlodzik, 1999) Homozygous Dl^RevF10 Ser^RX106 double mutant clones in the leg result in a failure to form joints. In most cases, the failure to form joints is an autonomous property of the mutant cells in the clone. (Rauskolb and Irvine, 1999) Homozygous clones induced early during eye development are associated with large abnormal patches of mutant tissue, with only rare mosaic ommatidia containing the normal number of 8 photoreceptors forming at the interface with wild-type tissue. Homozygous clones induced later during eye development can give rise to mosaic ommatidia with the normal number of photoreceptor cells. In those cases in which the R3/R4 pair is mosaic for Dl^RevF10, the mutant cell invariably develops as R4 and the wild-type cell develops as R3, irrespective of the location of the ommatidium within the eye, resulting in incorrect chiralities. In ommatidia in which both the R3 and R4 cells are mutant for Dl, the ommatidia can be symmetrical, or asymmetrical with the incorrect chiral form. The R8 cell body normally achieves an asymmetric position on the R3 rather than the R4 side of the ommatidium in wild-type flies. Frequent uncoupling of the R8 position from the R3/R4 asymmetry is seen in Dl^RevF10 mosaics. In these cases, R8 cells are usually found between R5/R6, occasionally found between R1/R6 and in rare cases are found between other cells. (Tomlinson and Struhl, 1999) The segmental border muscle progenitors do not segregate at all or do not divide properly. (Jagla et al., 1998) Neural differentiation does not occur in Dl^RevF10 Ser^RX106 double mutant clones in the eye disc, except near the clone margins. Where neural differentiation occurs near the clone margins, excessive numbers of R8 cells are seen. This phenotype resembles that seen for clones homozygous for Dl^- alone. (Ligoxygakis et al., 1998) Homozygous clones on the adult scutum have a tuft of densely packed bristles in the interior of the clone. Homozygous clones induced in the sensory organ lineage produce largely normal external bristle structures, with only 5% of homozygous macrochaetae having double shafts. Homozygous clones in the wing lead to modest expansions of vein tissue into the intervein area. (Zeng et al., 1998) Clonal cells in the eye disc are associated with neural hypertrophy involving clusters of R8 cells near the clone margin. Clones in the adult retina show ommatidia with excess photoreceptor cells only being found clone to the clone boundaries. Centre of the clone is undifferentiated or absent. (Baker and Yu, 1997) Homozygous embryos have an increased number of cardiac precursor cells. (Jagla et al., 1997) In mutant embryos carrying Dl^m39 segregation of neuroblasts 5-2 and 7-4 is normal at early stages. Embryos derived from germline clones exhibit disturbed segmentation in some segments and some 5-2 equivalence groups are fused. Segregation of the 5-2 and 7-4 neuroblast occurs in an irregular manner at later stages. Scer\GAL4^{arm.T:Hsim\VP16}-mediated expression of Dl^Scer\UAS.cHa fails to rescue the CNS and PNS differentiation defects: CNS shows an abnormal axonal scaffold and the PNS contains less cells that wild type. Each proneural cluster is neuralized from the very beginning of neurogenesis. Replacement of Dl zygotic expression using Scer\GAL4^{arm.T:Hsim\VP16}-mediated expression of Ser^Scer\UAS.cSa allows some rescue but the CNS is reduced at later stages. P precursors of the larvae PNS segregate as a group of cells, Scer\GAL4^{arm.T:Hsim\VP16}-mediated expression of Dl^Scer\UAS.cHa allows normal delamination of the individual cells. (Seugnet et al., 1997) Clonal analysis reveals that Dl is required for wing margin formation in the ventral but not the dorsal compartment. Sensory bristles on the anterior wing margin and the noninnervated hairs of the posterior wing margin are absent in clones (dorsal and ventral) that give rise to these structures. Clones also cause hypertrophy of wing veins. (Doherty et al., 1996) Mutant clones fail to form bristles. (Heitzler and Simpson, 1991)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement Reference Dl^RevF10 has visible phenotype, enhanceable by Scer\GAL4^dpp.blk1/Hs3st-B^{dsRNA.IR.Scer\UAS} (Kamimura et al., 2004)
NOT Enhanced by
Statement Reference Dl^RevF10 has visible \| somatic clone phenotype, non-enhanceable by heph⁰³⁴²⁹ (Dansereau et al., 2002)
NOT suppressed by
Statement Reference Dl^RevF10 has mitotic cell cycle defective \| somatic clone phenotype, non-suppressible by E2f^Scer\UAS.cNa/Dp^Scer\UAS.cDa/Scer\GAL4^GMR.PF/CycA^Scer\UAS.cWa/Scer\GAL4^GMR.PF (Baonza and Freeman, 2005) Dl^RevF10 has mitotic cell cycle defective \| somatic clone phenotype, non-suppressible by Ras85D^ΔC40B (Baonza and Freeman, 2005) Dl^RevF10 has visible \| somatic clone phenotype, non-suppressible by heph⁰³⁴²⁹ (Dansereau et al., 2002)
Enhancer of
Statement Reference Dl^RevF10/Dl[+] is an enhancer of planar polarity defective phenotype of dsh^hs.sev.B (Fanto and Mlodzik, 1999) Dl^RevF10/Dl[+] is an enhancer of planar polarity defective phenotype of fz^hs.sev (Fanto and Mlodzik, 1999) Dl^RevF10/Dl[+] is an enhancer of visible \| recessive phenotype of dx¹⁵² (Fuwa et al., 2006) Dl^RevF10/Dl[+] is an enhancer of visible phenotype of ed^slH8/ed^1X5 (Escudero et al., 2003) Dl^RevF10 is an enhancer of visible phenotype of Myt1^GMR.PP (Price et al., 2002)
NOT Enhancer of
Statement Reference Dl^RevF10/Dl[+] is a non-enhancer of planar polarity defective phenotype of Scer\GAL4^hs.2sev, nmo^Scer\UAS.cUa (Mirkovic et al., 2011) Dl^RevF10/Dl[+] is a non-enhancer of visible phenotype of Scer\GAL4^en-e16E, kermit^GS2053 (Djiane and Mlodzik, 2010) Dl^RevF10 is a non-enhancer of cell polarity defective phenotype of pk^pk.sev (Jenny et al., 2003) Dl^RevF10 is a non-enhancer of cell polarity defective phenotype of Scer\GAL4^hs.2sev, pk^{sple.Scer\UAS} (Jenny et al., 2003) Dl^RevF10 is a non-enhancer of visible phenotype of os^GMR.PB (Mukherjee et al., 2006)
NOT Suppressor of
Statement Reference Dl^RevF10/Dl[+] is a non-suppressor of planar polarity defective phenotype of Scer\GAL4^hs.2sev, nmo^Scer\UAS.cUa (Mirkovic et al., 2011) Dl^RevF10/Dl[+] is a non-suppressor of visible phenotype of Scer\GAL4^en-e16E, kermit^GS2053 (Djiane and Mlodzik, 2010) Dl^RevF10 is a non-suppressor of cell polarity defective phenotype of pk^pk.sev (Jenny et al., 2003) Dl^RevF10 is a non-suppressor of cell polarity defective phenotype of Scer\GAL4^hs.2sev, pk^{sple.Scer\UAS} (Jenny et al., 2003) Dl^RevF10 is a non-suppressor of visible phenotype of os^GMR.PB (Mukherjee et al., 2006)
Other
Statement Reference Dl^RevF10, N^Mcd5, Ser^RX82 has visible \| somatic clone phenotype (Ramain et al., 2001, Ramain et al., 2001, Ramain et al., 2001) Dl^RevF10, Ras85D^ΔC40B has neuroanatomy defective \| somatic clone phenotype (Baonza and Freeman, 2005) Dl^RevF10, Ser^RX82 has visible \| recessive phenotype (Zeng et al., 1998, Zeng et al., 1998)
Phenotype Manifest In
Enhanced by
Statement Reference Dl^RevF10 has anterior scutellar bristle phenotype, enhanceable by Scer\GAL4^dpp.blk1/Hs3st-B^{dsRNA.IR.Scer\UAS} (Kamimura et al., 2004) Dl^RevF10 has posterior scutellar bristle phenotype, enhanceable by Scer\GAL4^dpp.blk1/Hs3st-B^{dsRNA.IR.Scer\UAS} (Kamimura et al., 2004) Dl^RevF10 has sensory mother cell & mesothoracic tergum & third instar larva \| ectopic \| somatic clone phenotype, enhanceable by mib1^EY09780 (Pitsouli and Delidakis, 2005) Dl^RevF10 has sensory mother cell & mesothoracic tergum & third instar larva \| ectopic \| somatic clone phenotype, enhanceable by neur¹ (Pitsouli and Delidakis, 2005) Dl^RevF10 has sensory mother cell & mesothoracic tergum & third instar larva \| ectopic \| somatic clone phenotype, enhanceable by Ser^RX106 (Pitsouli and Delidakis, 2005) Dl^RevF10 has wing vein L3 phenotype, enhanceable by Scer\GAL4^dpp.blk1/Hs3st-B^{dsRNA.IR.Scer\UAS} (Kamimura et al., 2004)
NOT Enhanced by
Statement Reference Dl^RevF10 has wing vein \| somatic clone phenotype, non-enhanceable by heph⁰³⁴²⁹ (Dansereau et al., 2002)
Suppressed by
Statement Reference Dl^RevF10, Dl^Scer\UAS.cLa, Ser^RX106 has sensory mother cell & mesothoracic tergum & third instar larva \| ectopic \| somatic clone phenotype, suppressible by Scer\GAL4^{mat.αTub67C.T:Hsim\VP16} (Pitsouli and Delidakis, 2005) Dl^RevF10, Ser^RX106 has sensory mother cell & mesothoracic tergum & third instar larva \| ectopic \| somatic clone phenotype, suppressible by Ser^Scer\UAS.cGa/Scer\GAL4^{mat.αTub67C.T:Hsim\VP16} (Pitsouli and Delidakis, 2005) Dl^RevF10 has sensory mother cell & mesothoracic tergum & third instar larva \| ectopic \| somatic clone phenotype, suppressible \| partially by Scer\GAL4^{mat.αTub67C.T:Hsim\VP16}/fng^Scer\UAS.cKa (Pitsouli and Delidakis, 2005) Dl^RevF10 has sensory mother cell & mesothoracic tergum & third instar larva \| ectopic \| somatic clone phenotype, suppressible by Ser^RX106/Ser^Scer\UAS.cGa/Scer\GAL4^{mat.αTub67C.T:Hsim\VP16} (Pitsouli and Delidakis, 2005) Dl^RevF10 has sensory mother cell & mesothoracic tergum & third instar larva \| ectopic \| somatic clone phenotype, suppressible by Ser^Scer\UAS.cGa/Scer\GAL4^{mat.αTub67C.T:Hsim\VP16} (Pitsouli and Delidakis, 2005)
NOT suppressed by
Statement Reference Dl^RevF10, Ser^RX106 has external sensory organ phenotype, non-suppressible \| somatic clone by AP-1μ^SHE-11 (Benhra et al., 2011) Dl^RevF10 has eye disc \| somatic clone phenotype, non-suppressible by E2f^Scer\UAS.cNa/Dp^Scer\UAS.cDa/Scer\GAL4^GMR.PF/CycA^Scer\UAS.cWa/Scer\GAL4^GMR.PF (Baonza and Freeman, 2005) Dl^RevF10 has eye disc \| somatic clone phenotype, non-suppressible by Ras85D^ΔC40B (Baonza and Freeman, 2005) Dl^RevF10 has wing vein \| somatic clone phenotype, non-suppressible by heph⁰³⁴²⁹ (Dansereau et al., 2002)
Enhancer of
Statement Reference Dl^RevF10/Dl[+] is an enhancer of macrochaeta \| ectopic phenotype of ed^slH8/ed^1X5 (Escudero et al., 2003) Dl^RevF10/Dl[+] is an enhancer of ocellus phenotype of dx¹⁵² (Fuwa et al., 2006) Dl^RevF10/Dl[+] is an enhancer of ommatidium phenotype of dsh^hs.sev.B (Fanto and Mlodzik, 1999) Dl^RevF10/Dl[+] is an enhancer of ommatidium phenotype of fz^hs.sev (Fanto and Mlodzik, 1999) Dl^RevF10/Dl[+] is an enhancer of photoreceptor cell R4 phenotype of dsh^hs.sev.B (Fanto and Mlodzik, 1999) Dl^RevF10/Dl[+] is an enhancer of photoreceptor cell R4 phenotype of fz^hs.sev (Fanto and Mlodzik, 1999) Dl^RevF10/Dl[+] is an enhancer of wing vein phenotype of dx¹⁵² (Fuwa et al., 2006) Dl^RevF10 is an enhancer of eye phenotype of Myt1^GMR.PP (Price et al., 2002) Dl^RevF10 is an enhancer of photoreceptor cell R3 \| ectopic phenotype of Scer\GAL4^sev.PM181, stan^Scer\UAS.cUa (Das et al., 2002) Dl^RevF10 is an enhancer of photoreceptor cell R4 phenotype of Scer\GAL4^sev.PM181, stan^Scer\UAS.cUa (Das et al., 2002) Dl^RevF10 is an enhancer of sensory mother cell \| ectopic \| somatic clone phenotype of neur¹ (Pitsouli and Delidakis, 2005) Scer\GAL4^sev.PM181/Dl^RevF10 is an enhancer of ommatidium phenotype of stan^Scer\UAS.cUa (Das et al., 2002)
NOT Enhancer of
Statement Reference Dl^RevF10/Dl[+] is a non-enhancer of ommatidium phenotype of Scer\GAL4^hs.2sev, nmo^Scer\UAS.cUa (Mirkovic et al., 2011) Dl^RevF10/Dl[+] is a non-enhancer of wing hair phenotype of Scer\GAL4^en-e16E, kermit^GS2053 (Djiane and Mlodzik, 2010) Dl^RevF10 is a non-enhancer of eye phenotype of os^GMR.PB (Mukherjee et al., 2006) Dl^RevF10 is a non-enhancer of ommatidium phenotype of pk^pk.sev (Jenny et al., 2003) Dl^RevF10 is a non-enhancer of ommatidium phenotype of Scer\GAL4^hs.2sev, pk^{sple.Scer\UAS} (Jenny et al., 2003)
Suppressor of
Statement Reference Ser^RX106/Dl^RevF10 is a suppressor of tormogen cell \| supernumerary \| somatic clone phenotype of AP-1μ^SHE-11 (Benhra et al., 2011)
NOT Suppressor of
Statement Reference Dl^RevF10/Dl[+] is a non-suppressor of ommatidium phenotype of Scer\GAL4^hs.2sev, nmo^Scer\UAS.cUa (Mirkovic et al., 2011) Dl^RevF10/Dl[+] is a non-suppressor of wing hair phenotype of Scer\GAL4^en-e16E, kermit^GS2053 (Djiane and Mlodzik, 2010) Dl^RevF10 is a non-suppressor of eye phenotype of os^GMR.PB (Mukherjee et al., 2006) Dl^RevF10 is a non-suppressor of ommatidium phenotype of pk^pk.sev (Jenny et al., 2003) Dl^RevF10 is a non-suppressor of ommatidium phenotype of Scer\GAL4^hs.2sev, pk^{sple.Scer\UAS} (Jenny et al., 2003)
Other
Statement Reference Dl::N^{ΔECN.Scer\UAS}, Dl^RevF10, Scer\GAL4^byn-Gal4 has embryonic hindgut phenotype (Takashima et al., 2002, Takashima et al., 2002) Dl::N^{ΔECN.Scer\UAS}, Dl^RevF10, Scer\GAL4^byn-Gal4 has embryonic large intestine phenotype (Takashima et al., 2002, Takashima et al., 2002) Dl^RevF10, N^Mcd5, Ser^RX82 has microchaeta \| somatic clone phenotype (Ramain et al., 2001, Ramain et al., 2001, Ramain et al., 2001) Dl^RevF10, Ras85D^ΔC40B has eye disc \| somatic clone phenotype (Baonza and Freeman, 2005) Dl^RevF10, Scer\GAL4^{mat.αTub67C.T:Hsim\VP16}, Ser^RX106, Ser^Scer\UAS.cGa has sensory mother cell & mesothoracic tergum & third instar larva \| ectopic \| somatic clone phenotype (Pitsouli and Delidakis, 2005) Dl^RevF10, Ser[+]/Ser^RX82, eyg^M3-12/eyg[+] has eye phenotype (Chao et al., 2004) Dl^RevF10, Ser^RX82 has female germline stem cell \| germline clone phenotype (Ward et al., 2006) Dl^RevF10, Ser^RX82 has macrochaeta phenotype (Zeng et al., 1998, Zeng et al., 1998) Dl^RevF10, Ser^RX82 has scutum & macrochaeta phenotype (Zeng et al., 1998, Zeng et al., 1998) Dl^RevF10, Ser^RX82 has wing vein phenotype (Zeng et al., 1998, Zeng et al., 1998) Dl^RevF10, Ser^RX106 has external sensory organ \| somatic clone phenotype (Benhra et al., 2011) Dl^RevF10, Ser^RX106 has wing disc \| somatic clone phenotype (Rauskolb et al., 1999, Rauskolb et al., 1999) Dl^RevF10/Dl[+], dx¹⁵² has prothoracic tarsal segment 2 phenotype (Fuwa et al., 2006) Dl^RevF10/Dl[+], dx¹⁵² has prothoracic tarsal segment 3 phenotype (Fuwa et al., 2006) Dl^RevF10/Dl[+], Ser[+]/Ser^RX82, eyg^M3-12 has eye phenotype (Chao et al., 2004) Dl^RevF10/Dl[+], Ser^RX82, eyg^M3-12/eyg[+] has eye phenotype (Chao et al., 2004)
Additional Comments
Genetic Interactions
Statement Reference Dl[RevF10], Ser[RX106] double mutant clones in the adult thorax exhibit external sensory cell loss and an excess of neurons. A Dl[RevF10] Ser[RX106] double mutant background suppresses the extra socket cell phenotype seen in AP-47[SHE-11] thorax clones, instead producing the external sensory cell loss and excess neurons seen in Dl[RevF10] Ser[RX106] double mutants. (Benhra et al., 2011) Dl[RevF10]/+ enhances the wing vein thickening phenotype of dx[152] hemizygotes. The second and third tarsal segments of the forelegs are fused in 41% of the double mutants, a phenotype that is not seen in either dx[152] hemizygotes or Dl[RevF10]/+ animals. The double mutants show fusion of the ocelli in 37% of cases, compared to the 2% penetrance seen in dx[152] hemizygotes. (Fuwa et al., 2006) Dl^RevF10, Ser^RX82 mutant germline stem cells differentiate and leave the niche, resulting in an empty germarium phenotype when both germline stem cells are mutant. (Ward et al., 2006) Neural differentiation and the second mitotic wave are blocked in clones of cells in the eye disc simultaneously mutant for Dl[RevF10] and Ras85D[ΔC40B] in a Minute/+ background: mutant cells in the position of the second mitotic wave do not enter S phase and do not undergo mitosis, as in Dl[RevF10] clones. Co-overexpression of CycA[Scer\UAS.cWa], E2f[Scer\UAS.cNa] and Dp[Scer\UAS.cDa] (using Scer\GAL4[GMR.PF]) does not significantly rescue the S phase phenotype seen in Dl[RevF10] clones in a Minute/+ background. (Baonza and Freeman, 2005) Dl^RevF10 Ser^RX106 third instar nota clones exhibit at least eight sensory organ precursors (SOPs) in approximately 70.5% of SOP positions scored. Approximately 21.5% of SOP positions display between four and eight ectopic SOPs, while approximately 6% exhibit between one and four SOPs, with only 2% exhibiting one SOP, as in wild-type. Addition of the Dl^Scer\UAS.cLa transgene, under the control of Scer\GAL4^{mat.αTub67C.T:Hsim\VP16} partially rescues the ectopic SOP phenotype, with approximately 8.5% of SOP positions exhibiting between four and eight SOPs. Addition of the Ser^Scer\UAS.cGa transgene, under the control of Scer\GAL4^{mat.αTub67C.T:Hsim\VP16}, partially rescues the ectopic SOP phenotype, with approximately 26% of SOP positions exhibiting between one and four SOPs. Dl^RevF10 fng^Scer\UAS.cKa third instar nota clones (under the regulation of Scer\GAL4^{mat.αTub67C.T:Hsim\VP16}) exhibit at least eight sensory organ precursors (SOPs) in approximately 47% of SOP positions scored. Approximately 12.5% of SOP positions display between four and eight ectopic SOPs, while approximately 28% exhibit between one and four SOPs, with only 12.5% exhibiting one SOP, as in wild-type. Dl^RevF10 Ser^Scer\UAS.cGa third instar nota clones (under the regulation of Scer\GAL4^{mat.αTub67C.T:Hsim\VP16}) do not exhibit lateral inhibition defects in sensory organ precursor development. neur¹ Dl^RevF10 third instar nota clones exhibit at least eight sensory organ precursors (SOPs) in approximately 50% of SOP positions scored. Approximately 35% of SOP positions display between four and eight ectopic SOPs, while approximately 15% exhibit between one and four SOPs. No SOP positions appeared wild-type. Therefore neur enhances the Dl lateral inhibition phenotype. mib1^EY09780 Dl^RevF10 third instar nota clones exhibit at least eight sensory organ precursors (SOPs) in approximately 40% of SOP positions scored. Approximately 45% of SOP positions display between four and eight ectopic SOPs, while approximately 5% exhibit between one and four SOPs, with 10% exhibiting one SOP, as in wild-type. Addition of the Dl^Scer\UAS.cLa transgene, to Dl^RevF10 Ser^RX106 double mutant third instar nota clones, under the control of Scer\GAL4^{mat.αTub67C.T:Hsim\VP16}, partially rescues the ectopic SOP phenotype, with approximately 8.5% of SOP positions exhibiting between four and eight SOPs. (Pitsouli and Delidakis, 2005) Dl^RevF10, Ser^RX82 transheterozygotes have normal sized eyes, but eyg^M3-12; Dl^RevF10, Ser^RX82 triple heterozygous flies have small eyes. (Chao et al., 2004) Dl^RevF10/+ increases the number of extra macrochaetae on the notum seen in ed^1X5/ed^slH8 adults. (Escudero et al., 2003) Clones mutant for both heph⁰³⁴²⁹ and Dl^RevF10 have an autonomous thick veins phenotype and are associated with non-autonomous vein differentiation in neighbouring tissue, as occurs in Dl^RevF10 single mutant clones. (Dansereau et al., 2002) The proportion of symmetrical (R3/R3) ommatidia in the eyes of stan^Scer\UAS.cUa; Scer\GAL4^sev.PM181 flies is significantly enhanced by heterozygosity for Dl^RevF10. (Das et al., 2002) Expression of Dl::N^{ΔECN.Scer\UAS} under the control of Scer\GAL4^byn-Gal4 in a Dl^RevF10 mutant background results in boundary cell differentiation throughout the entire large intestine. (Takashima et al., 2002) Dl^RevF10,Ser^RX82 double mutant somatic clones in the follicle cells does not lead to any detectable phenotype. (Lopez-Schier and St. Johnston, 2001) N^Mcd5 Dl^RevF10 Ser^RX82 triple mutant clones do not develop microchaetae but produce epidermis. Macrochaetae in N^Mcd5 Dl^RevF10 double mutant clones develop as single bristles rather than as a neurogenic tuft. (Ramain et al., 2001) Dominantly enhances the eye-polarity phenotype of fz^hs.sev; most fz^hs.sev ommatidia show R3/R3-type symmetry in a Dl^RevF10 heterozygous background. Dominantly enhances the eye-polarity phenotype of dsh^hs.sev.B. (Fanto and Mlodzik, 1999) Dl^RevF10,Ser^RX106 double mutant somatic clones in wing discs fail to respect the dorsal ventral boundary. (Rauskolb et al., 1999) The number of ftz expressing MP2 neurons increases compared to wild-type (About 15 on each side of the midline, as compared to 2 in wild-type) in Dl^RevF10,Ser^RX82 homozygous embryos derived from Dl^RevF10,Ser^RX82 homozygous female germ-line clones (lacking both maternal and zygotic function). This is the same phenotype as seen in other Dl alleles alone. (Rusconi and Corbin, 1999) Dl^RevF10 Ser^RX82 double mutant clones in the eye behave in the same way as Dl^RevF10 single mutant clones. (Tomlinson and Struhl, 1999) Dl^RevF10 Ser^RX82 double homozygous clones on the adult scutum produce epidermal cells but not external bristle structures. Dl^RevF10 Ser^RX82 double homozygous clones induced in the sensory organ lineage frequently have bristles with double shafts (approximately 44% of homozygous macrochaetae have double shafts). Loss of external sensory structures (balding) is also seen. Dl^RevF10 Ser^RX82 double homozygous clones in the wing produce larger and more frequent thickening of the veins compared to single mutant Dl^RevF10 homozygous clones. (Zeng et al., 1998)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Rescued by	Dl^RevF10 is rescued by Dl^m39 (Kunisch et al., 1994)
Not rescued by	Dl^RevF10 is not rescued by Dl^Scer\UAS.cHa/Scer\GAL4^{arm.T:Hsim\VP16} (Seugnet et al., 1997)
Comments	Neurogenic phenotype, rescued by Dl^m39. (Kunisch et al., 1994)
Stocks ( 1 )
Bloomington	6300 Dl^RevF10 e^* Ser^RX82 P{neoFRT}82B/TM6B, Tb¹
Notes on Origin
Discoverer
	Revertant. (Haenlin, 1998.9.18)
External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 14 )
Reported As
Symbol Synonym	Delta^rev10 (Ohlstein and Spradling, 2007) Delta^revF10 (Seugnet et al., 1997, Poulton et al., 2011) Delta^RevF10 (Ward et al., 2006) Dl^F10 (Kunisch et al., 1994) Dl^rev10 (Sun and Deng, 2005, Pitsouli and Delidakis, 2005, Okajima, 2005, Overstreet et al., 2004, Chao et al., 2004, Shcherbata et al., 2004, Schaeffer et al., 2004, Escudero et al., 2003, Pavlopoulos et al., 2001, Dansereau et al., 2002, Deng et al., 2001, Tomlinson and Struhl, 2001, Lopez-Schier and St. Johnston, 2001, Lee et al., 2000, Tomlinson and Struhl, 1999, Rusconi and Corbin, 1999, Rauskolb et al., 1999, Rauskolb and Irvine, 1999, Rusconi and Corbin, 1998, Ligoxygakis et al., 1998, Micchelli et al., 1997, Baker and Yu, 1997, Doherty et al., 1996, Childress et al., 2006, Kamimura et al., 2004, Kamimura et al., 2004, Lei et al., 2003, Mirkovic et al., 2011, Glittenberg et al., 2006, Baonza and Freeman, 2005, Jaekel and Klein, 2006, Mao and Freeman, 2009, Fuwa et al., 2006, Wang et al., 2011) Dl^REV10 (Hori et al., 2004, Takashima et al., 2002) Dl^Rev10 (Baonza and Freeman, 2001, Sasaki et al., 2007, Okegbe and DiNardo, 2011, Thomas and van Meyel, 2007, Baonza and Freeman, 2005, Benhra et al., 2011, Banks et al., 2011) Dl^rev10e (Shcherbata et al., 2004, Windler and Bilder, 2010) Dl^rev (Xie et al., 2012) Dl^revF10 (Das et al., 2002, Fanto and Mlodzik, 1999, Haenlin, 1998.9.18, Lecourtois and Schweisguth, 1998, Seugnet et al., 1997, Childress et al., 2006, Djiane and Mlodzik, 2010, Leonardi et al., 2011) Dl^RevF10 (Ward et al., 2006, Ohlstein and Spradling, 2007, Kandachar et al., 2008, Babaoglan et al., 2009, Nicholson et al., 2011, Melicharek et al., 2008) RevF10 (Mukherjee et al., 2006) unnamed (Okajima et al., 2005)
Name Synonym	Delta^Rev10 (Benhra et al., 2011)
Secondary FlyBase IDs
	FBal0044741
References ( 77 )

Generate a list of	All references relating to this allele ( 77 )

List References by type	Research paper ( 71 ) Supplementary material ( 5 ) Personal communication to FlyBase ( 1 )
Recent research papers ( 9 )
	Couturier et al., 2012, Nat. Cell Biol. 14(2): 131--139 Endocytosis by Numb breaks Notch symmetry at cytokinesis. [FBrf0217937] Xie et al., 2012, Dev. Biol. 363(2): 399--412 Drosophila Epsin's role in Notch ligand cells requires three Epsin protein functions: The lipid binding function of the ENTH domain, a single Ubiquitin interaction motif, and a subset of the C-terminal protein binding modules. [FBrf0217494] Banks et al., 2011, PLoS ONE 6(3): e18259 The functions of auxilin and rab11 in Drosophila suggest that the fundamental role of ligand endocytosis in notch signaling cells is not recycling. [FBrf0213386] Benhra et al., 2011, Curr. Biol. 21(1): 87--95 AP-1 Controls the Trafficking of Notch and Sanpodo toward E-Cadherin Junctions in Sensory Organ Precursors. [FBrf0212697] Leonardi et al., 2011, Development 138(16): 3569--3578 Multiple O-glucosylation sites on Notch function as a buffer against temperature-dependent loss of signaling. [FBrf0214548] Nicholson et al., 2011, Development 138(2): 251--260 Notch-dependent expression of the archipelago ubiquitin ligase subunit in the Drosophila eye. [FBrf0212669] Okegbe and DiNardo, 2011, Development 138(7): 1259--1267 The endoderm specifies the mesodermal niche for the germline in Drosophila via Delta-Notch signaling. [FBrf0213233] Poulton et al., 2011, Development 138(9): 1737--1745 The microRNA pathway regulates the temporal pattern of Notch signaling in Drosophila follicle cells. [FBrf0213494] Wang et al., 2011, Dev. Biol. 350(2): 414--428 Notch signaling regulates neuroepithelial stem cell maintenance and neuroblast formation in Drosophila optic lobe development. [FBrf0212909] Show all research papers ...

Allele Dmel\DlRevF10

Allele Dmel\Dl^RevF10