Allele Dmel\Hsp83^e1D

General Information
Symbol	Dmel\Hsp83^e1D	Species	D. melanogaster
Name		FlyBase ID	FBal0029641
Feature type	allele	Associated gene	Dmel\Hsp83
Also Known As	e1D

Map ( GBrowse )
Allele class	hypomorphic allele - genetic evidence
Mutagen	ethyl methanesulfonate
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class	hypomorphic allele - genetic evidence (van der Straten et al., 1997)
Mutagen	ethyl methanesulfonate (van der Straten et al., 1997)
Mutations Mapped to the Genome

Type Location Additional Notes References point mutation 3L:3,194,611..3,194,611 reported_pr_change=S38L pr_change=S38L\|Hsp83-PA reported_na_change=C113T evidence=experimental na_change=C3194611T (Cutforth and Rubin, 1994)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Amino acid replacement: S38L. Nucleotide substitution: C113T. (van der Straten et al., 1997) Nucleotide substitution: C113T. Amino acid replacement: S38L. (Cutforth and Rubin, 1994)
Cytology
Phenotypic Data
Phenotypic Class
	lethal \| recessive (Simon et al., 1991, Yue et al., 1999) viable (with Hsp83^9J1) (Milton et al., 2005) viable (with Hsp83^13F3) (Milton et al., 2005) viable (with Hsp83^19F2) (Milton et al., 2005)
Phenotype Manifest In
	chaeta (Milton et al., 2005) Nebenkern (Yue et al., 1999) spermatid (Yue et al., 1999) spermatocyte (Yue et al., 1999) spermatozoon (Yue et al., 1999)
Detailed Description
	Statement Reference Hsp83^e1D has no effect on an invariant bristle trait (thoracic and scutellar bristles were analysed). Hsp83^e1D has a significant effect on a variable bristle trait (the sternopleural, orbital, ocellar and vibrissa and carina bristles were analysed); there is a significant difference in trait mean compared to the background strain in which the Hsp83^e1D mutation was induced. (Milton et al., 2005) Viable in transheterozygous combination with either Hsp83^13F3 or Hsp83^9J1; males are sterile and females are weakly fertile. Hsp83^e1D/Hsp83^13F3 and Hsp83^e1D/Hsp83^9J1 males show defects during spermatogenesis. The number and shape of spermatocytes within 16-cell cysts are mostly normal (5-10% are abnormal). Spermatids with variable number, size and shape of nuclei and nebenkern are seen. Needle-shaped crystals are present throughout developing spermatocytes and spermatids. Individualised sperm are present but they are not motile and are fragile. (Yue et al., 1999) Mutation fails to genetically interact with sev^{S11.T:Hsap\MYC}, 'sev³⁵¹', phl¹² or phl::tor^12D.hs.sev. (van der Straten et al., 1997) No effect the rough eye phenotype (formation of extra R7 cells) caused by P{sev-svp2} and no effect on the P{ro-svp1} rough eye phenotype (loss of one or more outer photoreceptors from many of the ommatidia). (Kramer et al., 1995)
External Data
Linkouts
Interactions
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement Reference 15+/-4% of Kr^If-1/+ flies born to Hsp83^e1D/+ mothers have outgrowths with ectopic vibrissae protruding from the ventral region of the eye, compared to less than 0.1% of Kr^If-1/+ flies born to isogenised wild-type mothers. (Sollars et al., 2003) Enhances the sev^B4 phenotype: disrupts sev^B4 protein signalling. (Simon et al., 1991)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Complements	Hsp83^9J1 (van der Straten et al., 1997) Hsp83^13F3 (van der Straten et al., 1997)
Fails to complement	Hsp83⁰⁸⁴⁴⁵ (Yue et al., 1999) Hsp83^e3A (van der Straten et al., 1997, Yue et al., 1999) Hsp83^e4A (van der Straten et al., 1997, Yue et al., 1999) Hsp83^e6A (van der Straten et al., 1997, Yue et al., 1999) Hsp83^e6D (van der Straten et al., 1997, Yue et al., 1999) Hsp83^P582 (Yue et al., 1999)
Rescued by	Hsp83^e1D/Hsp83^e6A is rescued by Hsp83^T:Avic\GFP (Tariq et al., 2009)
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

Comments
	No interaction with P{sev-svp1} or P{sev-svp2} exists. (Begemann et al., 1995)
External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 5 )
Reported As
Symbol Synonym	e1D (Rutherford and Lindquist, 1998, Tariq et al., 2009) E(sev)3A^e1D Hsap83^S38L (Kimura et al., 1997) Hsp83^e1D S38L (Yue et al., 1999)
Name Synonym
Secondary FlyBase IDs

References ( 11 )
Research paper	Tariq et al., 2009, Proc. Natl. Acad. Sci. U.S.A. 106(4): 1157--1162 Trithorax requires Hsp90 for maintenance of active chromatin at sites of gene expression. [FBrf0215368] Milton et al., 2005, Genetics 171(1): 119--130 Effect of E(sev) and Su(Raf) HsP83 mutants and trans-heterozygotes on bristle trait means and variation in Drosophila melanogaster. [FBrf0187651] Sollars et al., 2003, Nat. Genet. 33(1): 70--74 Evidence for an epigenetic mechanism by which Hsp90 acts as a capacitor for morphological evolution. [FBrf0156004] Yue et al., 1999, Genetics 151(3): 1065--1079 Genetic analysis of viable hsp90 alleles reveals a critical role in Drosophila spermatogenesis. [FBrf0108102] Rutherford and Lindquist, 1998, Nature 396(6709): 336--342 Hsp90 as a capacitor for morphological evolution. [FBrf0105920] Kimura et al., 1997, Genes Dev. 11(14): 1775--1785 Cdc37 is a molecular chaperone with specific functions in signal transduction. [FBrf0095430] van der Straten et al., 1997, EMBO J. 16(8): 1961--1969 The heat shock protein 83 (Hsp83) is required for Raf-mediated signalling in Drosophila. [FBrf0093811] Begemann et al., 1995, Development 121(1): 225--235 The Drosophila orphan nuclear receptor Seven-up requires the Ras pathway for its function in photoreceptor determination. [FBrf0076133] Kramer et al., 1995, Development 121(5): 1361--1372 Cell fate control in the Drosophila retina by the orphan receptor seven-up: its role in the decisions mediated by the ras signaling pathway. [FBrf0078827] Cutforth and Rubin, 1994, Cell 77(7): 1027--1036 Mutations in Hsp83 and cdc37 impair signaling by the sevenless receptor tyrosine kinase in Drosophila. [FBrf0072892] Simon et al., 1991, Cell 67(23): 701--716 Ras1 and a putative guanine nucleotide exchange factor perform crucial steps in signaling by the sevenless protein tyrosine kinase. [FBrf0053392]

Allele Dmel\Hsp83e1D

Allele Dmel\Hsp83^e1D