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General Information
Symbol
Dmel\Hsp83e6A
Species
D. melanogaster
Name
FlyBase ID
FBal0029644
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
e6A
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
point mutation
Nucleotide change:
C3196273T
Reported nucleotide change:
C1775T
Amino acid change:
S592F | Hsp83-PA; S592F | Hsp83-PB
Reported amino acid change:
S592F
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference
Amino acid replacement: S592F. Nucleotide substitution: C1775T.
Nucleotide substitution: C1775T. Amino acid replacement: S592F.
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference
57% of Hsp8308445/Hsp83e6A flies show a loss of locomotor behavioral rhythms under free-running conditions (after entrainment in light-dark cycles). Hsp8308445/Hsp83e6A flies show a reduced fraction of flies with rhythmic locomotor activity in light-dark cycles (50% rhythmic) compared to control flies (88%-95% rhythmic). Hsp8308445/Hsp83e6A mutants show highly variable locomotor activity patterns, including fully rhythmic, arrhythmic, and complex behavioral profiles, in contrast to wild-type flies, which show little variation among individuals.
Hsp8319F2/Hsp83e6A females have a series of ovarian defects. Approximately 75% of egg chambers are blocked at different developmental stages, not later than stage 9. The remaining egg chambers show a pronounced defect in the transfer of nurse cell cytoplasm to the oocyte; the nuclei of the nurse cells do not degenerate at the end of the dumping process, as occurs in wild-type egg chambers, but persist up to later stages of oogenesis. Mutant mature eggs are smaller in size than normal and have altered dorsal appendages.
Hsp83e6A flies show normal wing positions.
Hsp83e6A has no effect on an invariant bristle trait (thoracic and scutellar bristles were analysed). Hsp83e6A has a significant effect on a variable bristle trait (the sternopleural, orbital, ocellar and vibrissa and carina bristles were analysed); there is a significant difference in trait mean compared to the background strain in which the Hsp83e6A mutation was induced.
Viable in transheterozygous combination with Hsp839J1; males are sterile and females are weakly fertile. Viable in transheterozygous combination with Hsp8313F3; males and females are sterile. Hsp839J1/Hsp83e6A males show defects during spermatogenesis. The number and shape of spermatocytes within 16-cell cysts are mostly normal (5-10% are abnormal). Spermatids with variable number, size and shape of nuclei and nebenkern are seen. Needle-shaped crystals are present throughout developing spermatocytes and spermatids. Individualised sperm are present but they are not motile and are fragile. Hsp8308445/Hsp83e6A males show defects during spermatogenesis. Excessive numbers of primary spermatocytes are seen in many developing cysts. Spermatids with variable number, size and shape of nuclei and nebenkern are seen. Needle-shaped crystals are present throughout developing spermatocytes and spermatids. Individualised sperm are present but they are not motile and are fragile.
Mutation fails to genetically interact with sevS11.T:Hsap\MYC, 'sev351', phl12 or phl::tor12D.hs.sev.
External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressor of
Statement
Reference
Hsp83[+]/Hsp83e6A is a suppressor of visible | dominant | heat sensitive phenotype of Pc11
Hsp83[+]/Hsp83e6A is a suppressor of visible phenotype of Scer\GAL4Tub.PU, cswN308D.UASp
Phenotype Manifest In
Enhancer of
Statement
Reference
Hsp83[+]/Hsp83e6A is an enhancer of egg chamber phenotype of cup15
Hsp83[+]/Hsp83e6A is an enhancer of egg chamber phenotype of cup21
Suppressor of
Statement
Reference
Hsp83[+]/Hsp83e6A is a suppressor of sex comb | ectopic | heat sensitive phenotype of Pc11
Hsp83[+]/Hsp83e6A is a suppressor of wing vein | ectopic phenotype of Scer\GAL4Tub.PU, cswN308D.UASp
Other
Statement
Reference
Hsp83[+]/Hsp83e6A, cup21 has egg chamber phenotype
Hsp83[+]/Hsp83e6A, KrIf-1 has eye phenotype
Additional Comments
Genetic Interactions
Statement
Reference
The mechanical stress sensitivity of Tpisgk-1 animals is significantly suppressed if they are also mutant for Hsp83e6A.
The oogenesis defects seen in cup21 females are enhanced if they are also heterozygous for Hsp83e6A; double mutants show earlier egg chamber growth arrest and precocious degeneration of the nurse cell nuclei. The double mutant egg chambers are often misshapen, with egg chambers either unbudded or fused (seen by the presence of supernumerary nurse cell nuclei in the egg chamber). The oogenesis defects seen in cup15 females are enhanced if they are also heterozygous for Hsp83e6A.
Heterozygosity for Hsp83e6A suppresses the extra sex comb phenotype of Pc11/+ males at both 25[o]C and 29[o]C.
The mutant abdominal segmentation phenotype seen in females carrying nosΔBX in a nosBN/nosBN background is enhanced (abdominal segmentation is reduced) if the females also carry one copy of Hsp83e6A.
6+/-2% of KrIf-1/+ flies born to Hsp83e6A/+ mothers have outgrowths with ectopic vibrissae protruding from the ventral region of the eye, compared to less than 0.1% of KrIf-1/+ flies born to isogenised wild-type mothers.
Enhances the sevB4 phenotype: disrupts sevB4 protein signalling.
Xenogenetic Interactions
Statement
Reference
Expression of Hsap\PABPN117ala.Scer\UAS, under the control of Scer\GAL4Mhc.PW in a Hsp83e6A mutant background suppresses the wing posture phenotype seen in a wild-type background.
Complementation and Rescue Data
Images (0)
Mutant
Wild-type
Stocks (2)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (9)
References (19)