FlyBase Allele Report: Dmel\Hsp83[e6A]

General Information

Symbol

Dmel\Hsp83^e6A

Species

D. melanogaster

Name

FlyBase ID

FBal0029644

Feature type

allele

Associated gene

Dmel\Hsp83

Associated Insertion(s)

Carried in Construct

Also Known As

e6A, Hsp90^e6A

Key Links

Genomic Maps

JBrowse

Allele class

hypomorphic allele - genetic evidence

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

hypomorphic allele - genetic evidence

(van der Straten et al., 1997)

Mutagen

ethyl methanesulfonate

(van der Straten et al., 1997)

Progenitor genotype

Cytology

Description

Amino acid replacement: S592F. Nucleotide substitution: C1775T.

(van der Straten et al., 1997)

Nucleotide substitution: C1775T. Amino acid replacement: S592F.

(Cutforth and Rubin, 1994)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

3L:3,196,273..3,196,273 [+]

Nucleotide change:

C3196273T

Reported nucleotide change:

C1775T

Amino acid change:

S592F | Hsp83-PA; S592F | Hsp83-PB

Reported amino acid change:

S592F

(Cutforth and Rubin, 1994)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates tauopathy

modeled by Hsap\MAPT^{P301L.QUAS.0N4R}

(Praschberger et al., 2023)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal locomotor rhythm (with Hsp83⁰⁸⁴⁴⁵)

(Hung et al., 2009)

lethal | recessive

(Yue et al., 1999, Simon et al., 1991)

male sterile (with Hsp83^6-55)

(Bandura et al., 2013)

viable (with Hsp83^9J1)

(Milton et al., 2005)

Phenotype Manifest In

chaeta

(Milton et al., 2005)

dorsal appendage (with Hsp83^19F2)

(Pisa et al., 2009)

egg (with Hsp83^19F2)

(Pisa et al., 2009)

egg chamber (with Hsp83^19F2)

(Pisa et al., 2009)

Nebenkern

(Yue et al., 1999)

nurse cell (with Hsp83^19F2)

(Pisa et al., 2009)

primary spermatocyte cyst

(Yue et al., 1999)

spermatid

(Yue et al., 1999)

spermatocyte

(Yue et al., 1999)

spermatozoon

(Yue et al., 1999)

Detailed Description

Statement

Reference

57% of Hsp83⁰⁸⁴⁴⁵/Hsp83^e6A flies show a loss of locomotor behavioral rhythms under free-running conditions (after entrainment in light-dark cycles).

Hsp83⁰⁸⁴⁴⁵/Hsp83^e6A flies show a reduced fraction of flies with rhythmic locomotor activity in light-dark cycles (50% rhythmic) compared to control flies (88%-95% rhythmic).

Hsp83⁰⁸⁴⁴⁵/Hsp83^e6A mutants show highly variable locomotor activity patterns, including fully rhythmic, arrhythmic, and complex behavioral profiles, in contrast to wild-type flies, which show little variation among individuals.

(Hung et al., 2009)

Hsp83^19F2/Hsp83^e6A females have a series of ovarian defects. Approximately 75% of egg chambers are blocked at different developmental stages, not later than stage 9. The remaining egg chambers show a pronounced defect in the transfer of nurse cell cytoplasm to the oocyte; the nuclei of the nurse cells do not degenerate at the end of the dumping process, as occurs in wild-type egg chambers, but persist up to later stages of oogenesis. Mutant mature eggs are smaller in size than normal and have altered dorsal appendages.

(Pisa et al., 2009)

Hsp83^e6A flies show normal wing positions.

(Chartier et al., 2006)

Hsp83^e6A has no effect on an invariant bristle trait (thoracic and scutellar bristles were analysed). Hsp83^e6A has a significant effect on a variable bristle trait (the sternopleural, orbital, ocellar and vibrissa and carina bristles were analysed); there is a significant difference in trait mean compared to the background strain in which the Hsp83^e6A mutation was induced.

(Milton et al., 2005)

Viable in transheterozygous combination with Hsp83^9J1; males are sterile and females are weakly fertile. Viable in transheterozygous combination with Hsp83^13F3; males and females are sterile. Hsp83^9J1/Hsp83^e6A males show defects during spermatogenesis. The number and shape of spermatocytes within 16-cell cysts are mostly normal (5-10% are abnormal). Spermatids with variable number, size and shape of nuclei and nebenkern are seen. Needle-shaped crystals are present throughout developing spermatocytes and spermatids. Individualised sperm are present but they are not motile and are fragile. Hsp83⁰⁸⁴⁴⁵/Hsp83^e6A males show defects during spermatogenesis. Excessive numbers of primary spermatocytes are seen in many developing cysts. Spermatids with variable number, size and shape of nuclei and nebenkern are seen. Needle-shaped crystals are present throughout developing spermatocytes and spermatids. Individualised sperm are present but they are not motile and are fragile.

(Yue et al., 1999)

Mutation fails to genetically interact with sev^{S11.T:Hsap\MYC}, 'sev³⁵¹', phl¹² or phl::tor^12D.hs.sev.

(van der Straten et al., 1997)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Hsp83^e6A is a suppressor | partially of abnormal neurophysiology | adult stage phenotype of Hsap\MAPT^{P301L.QUAS.0N4R}, Ncra\QF^QF2w.nSyb

(Praschberger et al., 2023)

Hsp83^e6A is a suppressor of bang sensitive phenotype of Tpi^sgk-1

(Hrizo and Palladino, 2010)

Hsp83[+]/Hsp83^e6A is a suppressor of visible | dominant | heat sensitive phenotype of Pc¹¹

(Tariq et al., 2009)

Hsp83^e6A is a suppressor of visible phenotype of Hsap\PABPN1^17ala.UAS, Scer\GAL4^Mhc.PW

(Chartier et al., 2006)

Hsp83[+]/Hsp83^e6A is a suppressor of visible phenotype of Scer\GAL4^Tub.PU, csw^N308D.UASp

(Oishi et al., 2006)

Phenotype Manifest In

Enhancer of

Statement

Reference

Hsp83[+]/Hsp83^e6A is an enhancer of egg chamber phenotype of cup¹⁵

(Pisa et al., 2009)

Hsp83[+]/Hsp83^e6A is an enhancer of egg chamber phenotype of cup²¹

(Pisa et al., 2009)

Hsp83[+]/Hsp83^e6A is an enhancer | maternal effect of embryonic abdominal segment | maternal effect phenotype of nanos^ΔBX, nanos^BN

(Song et al., 2007)

Suppressor of

Statement

Reference

Hsp83^e6A is a suppressor | partially of retina | adult stage phenotype of Hsap\MAPT^{P301L.QUAS.0N4R}, Ncra\QF^QF2w.nSyb

(Praschberger et al., 2023)

Hsp83[+]/Hsp83^e6A is a suppressor of sex comb | ectopic | heat sensitive phenotype of Pc¹¹

(Tariq et al., 2009)

Hsp83^e6A is a suppressor of wing phenotype of Hsap\PABPN1^17ala.UAS, Scer\GAL4^Mhc.PW

(Chartier et al., 2006)

Hsp83[+]/Hsp83^e6A is a suppressor of wing vein | ectopic phenotype of Scer\GAL4^Tub.PU, csw^N308D.UASp

(Oishi et al., 2006)

Other

Statement

Reference

Hsp83[+]/Hsp83^e6A, cup²¹ has egg chamber phenotype

(Pisa et al., 2009)

Hsp83^e6A, Kr^If-1 has eye phenotype

(Sollars et al., 2003)

Hsp83[+]/Hsp83^e6A, Kr^If-1 has eye phenotype

(Sollars et al., 2003)

Additional Comments

Genetic Interactions

Statement

Reference

The mechanical stress sensitivity of Tpi^sgk-1 animals is significantly suppressed if they are also mutant for Hsp83^e6A.

(Hrizo and Palladino, 2010)

The oogenesis defects seen in cup²¹ females are enhanced if they are also heterozygous for Hsp83^e6A; double mutants show earlier egg chamber growth arrest and precocious degeneration of the nurse cell nuclei. The double mutant egg chambers are often misshapen, with egg chambers either unbudded or fused (seen by the presence of supernumerary nurse cell nuclei in the egg chamber).

The oogenesis defects seen in cup¹⁵ females are enhanced if they are also heterozygous for Hsp83^e6A.

(Pisa et al., 2009)

Heterozygosity for Hsp83^e6A suppresses the extra sex comb phenotype of Pc¹¹/+ males at both 25[o]C and 29[o]C.

(Tariq et al., 2009)

The mutant abdominal segmentation phenotype seen in females carrying nos^ΔBX in a nos^BN/nos^BN background is enhanced (abdominal segmentation is reduced) if the females also carry one copy of Hsp83^e6A.

(Song et al., 2007)

6+/-2% of Kr^If-1/+ flies born to Hsp83^e6A/+ mothers have outgrowths with ectopic vibrissae protruding from the ventral region of the eye, compared to less than 0.1% of Kr^If-1/+ flies born to isogenised wild-type mothers.

(Sollars et al., 2003)

Enhances the sev^B4 phenotype: disrupts sev^B4 protein signalling.

(Simon et al., 1991)

Xenogenetic Interactions

Statement

Reference

Expression of Hsap\PABPN1^{17ala.Scer\UAS}, under the control of Scer\GAL4^Mhc.PW in a Hsp83^e6A mutant background suppresses the wing posture phenotype seen in a wild-type background.

(Chartier et al., 2006)

Complementation and Rescue Data

Complements

Hsp83^9J1

(van der Straten et al., 1997)

Hsp83^13F3

(van der Straten et al., 1997)

Partially complements

Hsp83⁰⁸⁴⁴⁵

(Yue et al., 1999)

Fails to complement

(Yue et al., 1999)

(Yue et al., 1999)

(Yue et al., 1999)

(Yue et al., 1999)

(Yue et al., 1999)

(van der Straten et al., 1997)

Hsp83^e4A

(van der Straten et al., 1997)

Hsp83^e3A

(van der Straten et al., 1997)

Hsp83^e1D

(van der Straten et al., 1997)

Rescued by

Hsp83^e1D/Hsp83^e6A is rescued by Hsp83^GFP

(Tariq et al., 2009)

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

36576

w^*; Hsp83^e6A/TM6B, Tb¹

Kyoto

108372

w^*; Hsp83^e6A/TM6B, Tb¹

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (10)

Reported As

Symbol Synonym

E(sev)3A^e6A

E6A

(Hrizo and Palladino, 2010)

Hsap83^S592F

(Kimura et al., 1997)

Hsp83^S592F

(Song et al., 2007)

Hsp83^e6A

(Snir et al., 2024, Praschberger et al., 2023, Bandura et al., 2013, Pisa et al., 2009, Christensen et al., 2008.12.28, Chartier et al., 2006, Oishi et al., 2006)

Hsp83^e6a

(Shaw et al., 2002)

Hsp90^e6A

(Lee et al., 2023, Lauwers et al., 2018)

S592F

(Yue et al., 1999)

e6A

(Bandura et al., 2013, Tariq et al., 2009, Rutherford and Lindquist, 1998)

hsp83^e6A

(Huang and Wang, 2018, Hung et al., 2009)

Name Synonyms

Secondary FlyBase IDs

References (24)

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