Allele Dmel\Hsp83^e6A

General Information
Symbol	Dmel\Hsp83^e6A	Species	D. melanogaster
Name		FlyBase ID	FBal0029644
Feature type	allele	Associated gene	Dmel\Hsp83
Also Known As	e6A

Map ( GBrowse )
Allele class	hypomorphic allele - genetic evidence
Mutagen	ethyl methanesulfonate
Recent Updates
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FB2013_03
FB2013_02
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Nature of the Allele
Allele class	hypomorphic allele - genetic evidence (van der Straten et al., 1997)
Mutagen	ethyl methanesulfonate (van der Straten et al., 1997)
Mutations Mapped to the Genome

Type Location Additional Notes References point mutation 3L:3,196,273..3,196,273 evidence=experimental pr_change=S592F\|Hsp83-PA reported_na_change=C1775T reported_pr_change=S592F na_change=C3196273T (Cutforth and Rubin, 1994)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Amino acid replacement: S592F. Nucleotide substitution: C1775T. (van der Straten et al., 1997) Nucleotide substitution: C1775T. Amino acid replacement: S592F. (Cutforth and Rubin, 1994)
Cytology
Phenotypic Data
Phenotypic Class
	lethal \| recessive (Simon et al., 1991, Yue et al., 1999) locomotor rhythm defective (with Hsp83⁰⁸⁴⁴⁵) (Hung et al., 2009) viable (with Hsp83^9J1) (Milton et al., 2005)
Phenotype Manifest In
	chaeta (Milton et al., 2005) dorsal appendage (with Hsp83^19F2) (Pisa et al., 2009) egg (with Hsp83^19F2) (Pisa et al., 2009) egg chamber (with Hsp83^19F2) (Pisa et al., 2009) Nebenkern (Yue et al., 1999) nurse cell (with Hsp83^19F2) (Pisa et al., 2009) primary spermatocyte cyst (Yue et al., 1999) spermatid (Yue et al., 1999) spermatocyte (Yue et al., 1999) spermatozoon (Yue et al., 1999)
Detailed Description
	Statement Reference 57% of Hsp83[08445]/Hsp83[e6A] flies show a loss of locomotor behavioral rhythms under free-running conditions (after entrainment in light-dark cycles). Hsp83[08445]/Hsp83[e6A] flies show a reduced fraction of flies with rhythmic locomotor activity in light-dark cycles (50% rhythmic) compared to control flies (88%-95% rhythmic). Hsp83[08445]/Hsp83[e6A] mutants show highly variable locomotor activity patterns, including fully rhythmic, arrhythmic, and complex behavioral profiles, in contrast to wild-type flies, which show little variation among individuals. (Hung et al., 2009) Hsp83[19F2]/Hsp83[e6A] females have a series of ovarian defects. Approximately 75% of egg chambers are blocked at different developmental stages, not later than stage 9. The remaining egg chambers show a pronounced defect in the transfer of nurse cell cytoplasm to the oocyte; the nuclei of the nurse cells do not degenerate at the end of the dumping process, as occurs in wild-type egg chambers, but persist up to later stages of oogenesis. Mutant mature eggs are smaller in size than normal and have altered dorsal appendages. (Pisa et al., 2009) Hsp83^e6A flies show normal wing positions. (Chartier et al., 2006) Hsp83^e6A has no effect on an invariant bristle trait (thoracic and scutellar bristles were analysed). Hsp83^e6A has a significant effect on a variable bristle trait (the sternopleural, orbital, ocellar and vibrissa and carina bristles were analysed); there is a significant difference in trait mean compared to the background strain in which the Hsp83^e6A mutation was induced. (Milton et al., 2005) Viable in transheterozygous combination with Hsp83^9J1; males are sterile and females are weakly fertile. Viable in transheterozygous combination with Hsp83^13F3; males and females are sterile. Hsp83^9J1/Hsp83^e6A males show defects during spermatogenesis. The number and shape of spermatocytes within 16-cell cysts are mostly normal (5-10% are abnormal). Spermatids with variable number, size and shape of nuclei and nebenkern are seen. Needle-shaped crystals are present throughout developing spermatocytes and spermatids. Individualised sperm are present but they are not motile and are fragile. Hsp83⁰⁸⁴⁴⁵/Hsp83^e6A males show defects during spermatogenesis. Excessive numbers of primary spermatocytes are seen in many developing cysts. Spermatids with variable number, size and shape of nuclei and nebenkern are seen. Needle-shaped crystals are present throughout developing spermatocytes and spermatids. Individualised sperm are present but they are not motile and are fragile. (Yue et al., 1999) Mutation fails to genetically interact with sev^{S11.T:Hsap\MYC}, 'sev³⁵¹', phl¹² or phl::tor^12D.hs.sev. (van der Straten et al., 1997)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressor of
Statement Reference Hsp83[+]/Hsp83^e6A is a suppressor of visible \| dominant \| heat sensitive phenotype of Pc¹¹ (Tariq et al., 2009) Hsp83[+]/Hsp83^e6A is a suppressor of visible phenotype of Scer\GAL4^tub.PU, csw^{N308D.Scer\UAS.P\T} (Oishi et al., 2006) Hsp83^e6A is a suppressor of bang sensitive phenotype of Tpi^sgk-1/Tpi^sgk-1 (Hrizo and Palladino, 2010) Hsp83^e6A is a suppressor of visible phenotype of Hsap\PABPN1^{17ala.Scer\UAS}, Scer\GAL4^Mhc.PW (Chartier et al., 2006)
Phenotype Manifest In
Enhancer of
Statement Reference Hsp83[+]/Hsp83^e6A is an enhancer \| maternal effect of embryonic abdominal segment \| maternal effect phenotype of nos^ΔBX, nos^BN (Song et al., 2007) Hsp83[+]/Hsp83^e6A is an enhancer of egg chamber phenotype of cup¹⁵ (Pisa et al., 2009) Hsp83[+]/Hsp83^e6A is an enhancer of egg chamber phenotype of cup²¹ (Pisa et al., 2009)
Suppressor of
Statement Reference Hsp83[+]/Hsp83^e6A is a suppressor of sex comb \| ectopic \| heat sensitive phenotype of Pc¹¹ (Tariq et al., 2009) Hsp83[+]/Hsp83^e6A is a suppressor of wing vein \| ectopic phenotype of Scer\GAL4^tub.PU, csw^{N308D.Scer\UAS.P\T} (Oishi et al., 2006) Hsp83^e6A is a suppressor of wing phenotype of Hsap\PABPN1^{17ala.Scer\UAS}, Scer\GAL4^Mhc.PW (Chartier et al., 2006)
Other
Statement Reference Hsp83[+]/Hsp83^e6A, cup²¹ has egg chamber phenotype (Pisa et al., 2009) Hsp83[+]/Hsp83^e6A, Kr^If-1 has eye phenotype (Sollars et al., 2003) Hsp83^e6A, Kr^If-1 has eye phenotype (Sollars et al., 2003)
Additional Comments
Genetic Interactions
Statement Reference The mechanical stress sensitivity of Tpi[sgk-1] animals is significantly suppressed if they are also mutant for Hsp83[e6A]. (Hrizo and Palladino, 2010) The oogenesis defects seen in cup[21] females are enhanced if they are also heterozygous for Hsp83[e6A]; double mutants show earlier egg chamber growth arrest and precocious degeneration of the nurse cell nuclei. The double mutant egg chambers are often misshapen, with egg chambers either unbudded or fused (seen by the presence of supernumerary nurse cell nuclei in the egg chamber). The oogenesis defects seen in cup[15] females are enhanced if they are also heterozygous for Hsp83[e6A]. (Pisa et al., 2009) Heterozygosity for Hsp83[e6A] suppresses the extra sex comb phenotype of Pc[11]/+ males at both 25[o]C and 29[o]C. (Tariq et al., 2009) The mutant abdominal segmentation phenotype seen in females carrying nos^ΔBX in a nos^BN/nos^BN background is enhanced (abdominal segmentation is reduced) if the females also carry one copy of Hsp83^e6A. (Song et al., 2007) 6+/-2% of Kr^If-1/+ flies born to Hsp83^e6A/+ mothers have outgrowths with ectopic vibrissae protruding from the ventral region of the eye, compared to less than 0.1% of Kr^If-1/+ flies born to isogenised wild-type mothers. (Sollars et al., 2003) Enhances the sev^B4 phenotype: disrupts sev^B4 protein signalling. (Simon et al., 1991)
Xenogenetic Interactions
Statement Reference Expression of Hsap\PABPN1^{17ala.Scer\UAS}, under the control of Scer\GAL4^Mhc.PW in a Hsp83^e6A mutant background suppresses the wing posture phenotype seen in a wild-type background. (Chartier et al., 2006)
Complementation & Rescue Data
Complements	Hsp83^9J1 (van der Straten et al., 1997) Hsp83^13F3 (van der Straten et al., 1997)
Partially complements	Hsp83⁰⁸⁴⁴⁵ (Yue et al., 1999)
Fails to complement	Hsp83^e1D (van der Straten et al., 1997, Yue et al., 1999) Hsp83^e3A (van der Straten et al., 1997, Yue et al., 1999) Hsp83^e4A (van der Straten et al., 1997, Yue et al., 1999) Hsp83^e6D (van der Straten et al., 1997, Yue et al., 1999) Hsp83^P582 (Yue et al., 1999)
Rescued by	Hsp83^e1D/Hsp83^e6A is rescued by Hsp83^T:Avic\GFP (Tariq et al., 2009)
Comments
Stocks ( 2 )
Bloomington	36576 w^* Hsp83^e6A/TM6B, Tb¹
Kyoto	108372 w^*; Hsp83^e6A/TM6B, Tb¹
Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 9 )
Reported As
Symbol Synonym	e6A (Rutherford and Lindquist, 1998, Tariq et al., 2009) E6A (Hrizo and Palladino, 2010) E(sev)3A^e6A Hsap83^S592F (Kimura et al., 1997) Hsp83^e6a (Shaw et al., 2002) Hsp83^e6A (Chartier et al., 2006, Christensen et al., 2008.12.28, Pisa et al., 2009, Oishi et al., 2006) hsp83^e6A (Hung et al., 2009) Hsp83^S592F (Song et al., 2007) S592F (Yue et al., 1999)
Name Synonym
Secondary FlyBase IDs

References ( 18 )
Research paper	Hrizo and Palladino, 2010, Neurobiol. Disease 40(3): 676--683 Hsp70- and Hsp90-mediated proteasomal degradation underlies TPI(sugarkill) pathogenesis in Drosophila. [FBrf0212050] Hung et al., 2009, J. Biol. Rhythms 24(3): 183--192 HSP90, a Capacitor of Behavioral Variation. [FBrf0207993] Pisa et al., 2009, Gene 432(1-2): 67--74 The molecular chaperone Hsp90 is a component of the cap-binding complex and interacts with the translational repressor Cup during Drosophila oogenesis. [FBrf0206831] Tariq et al., 2009, Proc. Natl. Acad. Sci. U.S.A. 106(4): 1157--1162 Trithorax requires Hsp90 for maintenance of active chromatin at sites of gene expression. [FBrf0215368] Song et al., 2007, Genetics 176(4): 2213--2222 The molecular chaperone Hsp90 is required for mRNA localization in Drosophila melanogaster embryos. [FBrf0202066] Chartier et al., 2006, EMBO J. 25(10): 2253--2262 A Drosophila model of oculopharyngeal muscular dystrophy reveals intrinsic toxicity of PABPN1. [FBrf0190356] Oishi et al., 2006, Hum. Mol. Genet. 15(4): 543--553 Transgenic Drosophila models of Noonan syndrome causing PTPN11 gain-of-function mutations. [FBrf0190828] Milton et al., 2005, Genetics 171(1): 119--130 Effect of E(sev) and Su(Raf) HsP83 mutants and trans-heterozygotes on bristle trait means and variation in Drosophila melanogaster. [FBrf0187651] Atallah et al., 2004, Evol. Dev. 6(2): 114--122 The environmental and genetic regulation of obake expressivity: morphogenetic fields as evolvable systems. [FBrf0178980] Sollars et al., 2003, Nat. Genet. 33(1): 70--74 Evidence for an epigenetic mechanism by which Hsp90 acts as a capacitor for morphological evolution. [FBrf0156004] Shaw et al., 2002, Nature 417(6886): 287--291 Stress response genes protect against lethal effects of sleep deprivation in Drosophila. [FBrf0149122] Yue et al., 1999, Genetics 151(3): 1065--1079 Genetic analysis of viable hsp90 alleles reveals a critical role in Drosophila spermatogenesis. [FBrf0108102] Rutherford and Lindquist, 1998, Nature 396(6709): 336--342 Hsp90 as a capacitor for morphological evolution. [FBrf0105920] Kimura et al., 1997, Genes Dev. 11(14): 1775--1785 Cdc37 is a molecular chaperone with specific functions in signal transduction. [FBrf0095430] van der Straten et al., 1997, EMBO J. 16(8): 1961--1969 The heat shock protein 83 (Hsp83) is required for Raf-mediated signalling in Drosophila. [FBrf0093811] Cutforth and Rubin, 1994, Cell 77(7): 1027--1036 Mutations in Hsp83 and cdc37 impair signaling by the sevenless receptor tyrosine kinase in Drosophila. [FBrf0072892] Simon et al., 1991, Cell 67(23): 701--716 Ras1 and a putative guanine nucleotide exchange factor perform crucial steps in signaling by the sevenless protein tyrosine kinase. [FBrf0053392]
Personal communication to FlyBase	Christensen et al., 2008.12.28, Isolation and characterization of Df(3L)BSC672. Isolation and characterization of Df(3L)BSC672. [FBrf0206420]

Allele Dmel\Hsp83e6A

Allele Dmel\Hsp83^e6A