|Feature type||allele||Associated gene||Dmel\Hsp83|
|Also Known As||e6A|
|Map ( GBrowse )|
|Allele class||hypomorphic allele - genetic evidence|
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|Nature of the Allele|
|Mutations Mapped to the Genome|
|Associated Sequence Data|
|Nature of the lesion|
Amino acid replacement: S592F. Nucleotide substitution: C1775T.
Nucleotide substitution: C1775T. Amino acid replacement: S592F.
|Phenotype Manifest In|
57% of Hsp83/Hsp83[e6A] flies show a loss of locomotor behavioral rhythms under free-running conditions (after entrainment in light-dark cycles). Hsp83/Hsp83[e6A] flies show a reduced fraction of flies with rhythmic locomotor activity in light-dark cycles (50% rhythmic) compared to control flies (88%-95% rhythmic). Hsp83/Hsp83[e6A] mutants show highly variable locomotor activity patterns, including fully rhythmic, arrhythmic, and complex behavioral profiles, in contrast to wild-type flies, which show little variation among individuals.
Hsp83[19F2]/Hsp83[e6A] females have a series of ovarian defects. Approximately 75% of egg chambers are blocked at different developmental stages, not later than stage 9. The remaining egg chambers show a pronounced defect in the transfer of nurse cell cytoplasm to the oocyte; the nuclei of the nurse cells do not degenerate at the end of the dumping process, as occurs in wild-type egg chambers, but persist up to later stages of oogenesis. Mutant mature eggs are smaller in size than normal and have altered dorsal appendages.
Hsp83e6A has no effect on an invariant bristle trait (thoracic and scutellar bristles were analysed). Hsp83e6A has a significant effect on a variable bristle trait (the sternopleural, orbital, ocellar and vibrissa and carina bristles were analysed); there is a significant difference in trait mean compared to the background strain in which the Hsp83e6A mutation was induced.
Viable in transheterozygous combination with Hsp839J1; males are sterile and females are weakly fertile. Viable in transheterozygous combination with Hsp8313F3; males and females are sterile. Hsp839J1/Hsp83e6A males show defects during spermatogenesis. The number and shape of spermatocytes within 16-cell cysts are mostly normal (5-10% are abnormal). Spermatids with variable number, size and shape of nuclei and nebenkern are seen. Needle-shaped crystals are present throughout developing spermatocytes and spermatids. Individualised sperm are present but they are not motile and are fragile. Hsp8308445/Hsp83e6A males show defects during spermatogenesis. Excessive numbers of primary spermatocytes are seen in many developing cysts. Spermatids with variable number, size and shape of nuclei and nebenkern are seen. Needle-shaped crystals are present throughout developing spermatocytes and spermatids. Individualised sperm are present but they are not motile and are fragile.
|Phenotype Manifest In|
Hsp83[+]/Hsp83e6A is an enhancer | maternal effect of embryonic abdominal segment | maternal effect phenotype of nosΔBX, nosBN
Hsp83[+]/Hsp83e6A is a suppressor of wing vein | ectopic phenotype of Scer\GAL4tub.PU, cswN308D.Scer\UAS.P\T
The mechanical stress sensitivity of Tpi[sgk-1] animals is significantly suppressed if they are also mutant for Hsp83[e6A].
The oogenesis defects seen in cup females are enhanced if they are also heterozygous for Hsp83[e6A]; double mutants show earlier egg chamber growth arrest and precocious degeneration of the nurse cell nuclei. The double mutant egg chambers are often misshapen, with egg chambers either unbudded or fused (seen by the presence of supernumerary nurse cell nuclei in the egg chamber). The oogenesis defects seen in cup females are enhanced if they are also heterozygous for Hsp83[e6A].
Heterozygosity for Hsp83[e6A] suppresses the extra sex comb phenotype of Pc/+ males at both 25[o]C and 29[o]C.
The mutant abdominal segmentation phenotype seen in females carrying nosΔBX in a nosBN/nosBN background is enhanced (abdominal segmentation is reduced) if the females also carry one copy of Hsp83e6A.
6+/-2% of KrIf-1/+ flies born to Hsp83e6A/+ mothers have outgrowths with ectopic vibrissae protruding from the ventral region of the eye, compared to less than 0.1% of KrIf-1/+ flies born to isogenised wild-type mothers.
|Complementation & Rescue Data|
|Fails to complement|
|Stocks ( 2 )|
|Notes on Origin|
|External Crossreferences & Linkouts|
|Synonyms & Secondary IDs ( 9 )|
|Secondary FlyBase IDs|
|References ( 18 )|
|Personal communication to FlyBase|