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General Information
Symbol
Dmel\Hsp83e6D
Species
D. melanogaster
Name
FlyBase ID
FBal0029645
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
e6D
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
point mutation
Nucleotide change:
G3195447A
Reported nucleotide change:
G949A
Amino acid change:
E317K | Hsp83-PA; E317K | Hsp83-PB
Reported amino acid change:
E317K
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference
Amino acid replacement: E317K. Nucleotide substitution: G949A.
Nucleotide substitution: G949A. Amino acid replacement: E317K.
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference
When challenged with a large phase advance in their environmental temperature cycle in DD, Hsp83e6D/Hsp8308445 mutant flies exhibit a significant reduction in the number of 'transition days' associated with the process of phase-resetting daily locomotor activity.
67% of Hsp8308445/Hsp83e6D flies show a loss of locomotor behavioral rhythms under free-running conditions (after entrainment in light-dark cycles). Hsp8308445/Hsp83e6D flies show a reduced fraction of flies with rhythmic locomotor activity in light-dark cycles (49% rhythmic) compared to control flies (88%-95% rhythmic). Hsp8308445/Hsp83e6D mutants show highly variable locomotor activity patterns, including fully rhythmic, arrhythmic, and complex behavioral profiles, in contrast to wild-type flies, which show little variation among individuals.
Hsp83e6D/Hsp8308445 females are reasonably healthy and produce large numbers of eggs. Very few of the embryos derived from these eggs cellularise. The 1-2% of the embryos that eventually secrete cuticle have on average 3 abdominal segments rather than the normal 8.
Hsp83e6D has no effect on an invariant bristle trait (thoracic and scutellar bristles were analysed). Hsp83e6D has a significant effect on a variable bristle trait (the sternopleural, orbital, ocellar and vibrissa and carina bristles were analysed); there is a significant difference in trait mean compared to the background strain in which the Hsp83e6D mutation was induced.
Viable in transheterozygous combination with either Hsp839J1; males are sterile and females are weakly fertile. Hsp8308445/Hsp83e6D and Hsp83e6D/Hsp839J1 males show defects during spermatogenesis. Excessive numbers of primary spermatocytes are seen in many developing cysts. Spermatids with variable number, size and shape of nuclei and nebenkern are seen. Needle-shaped crystals are present throughout developing spermatocytes and spermatids. Individualised sperm are present but they are not motile and are fragile.
Mutation show strong genetic interaction with sevS11.T:Hsap\MYC, 'sev351', phl12 or phl::tor12D.hs.sev.
External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressor of
Statement
Reference
Hsp83e6D/Hsp83[+] is a suppressor of visible phenotype of Scer\GAL4Tub.PU, cswN308D.UASp
Phenotype Manifest In
Enhancer of
Statement
Reference
Hsp83e6D/Hsp83[+] is an enhancer of arista | supernumerary phenotype of obk1
Suppressor of
Statement
Reference
Hsp83e6D is a suppressor of wing vein phenotype of EgfrE1
Hsp83e6D is a suppressor of eye phenotype of Raf98
Hsp83e6D/Hsp83[+] is a suppressor of wing vein | ectopic phenotype of Scer\GAL4Tub.PU, cswN308D.UASp
Other
Statement
Reference
Additional Comments
Genetic Interactions
Statement
Reference
A Hsp83e6D background suppresses the abnormal wing vein morphology found in EgfrE1 heterozygous flies. The presence of Hsp83e6D reduces the rough eye morphology seen in Raf98 flies.
The mutant abdominal segmentation phenotype seen in females carrying nosΔBX in a nosBN/nosBN background is enhanced (abdominal segmentation is reduced) if the females also carry one copy of Hsp83e6D.
13+/-4% of KrIf-1/+ flies born to Hsp83e6D/+ mothers have outgrowths with ectopic vibrissae protruding from the ventral region of the eye, compared to less than 0.1% of KrIf-1/+ flies born to isogenised wild-type mothers.
Enhances the sevB4 phenotype: disrupts sevB4 protein signalling.
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Images (0)
Mutant
Wild-type
Stocks (2)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (8)
Reported As
Name Synonyms
Secondary FlyBase IDs
    References (18)