Allele Dmel\Trl⁶²

General Information
Symbol	Dmel\Trl⁶²	Species	D. melanogaster
Name		FlyBase ID	FBal0029713
Feature type	allele	Associated gene	Dmel\Trl

Map ( GBrowse )	Untitled Document
Allele class
Mutagen	Delta2-3
Recent Updates
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FB2013_03
FB2013_02
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Nature of the Allele
Allele class
Mutagen	Delta2-3 (Dorn et al., 1993)
Mutations Mapped to the Genome

Type Location Additional Notes References transposable element insertion site 3L:14,751,032..14,751,032 3L:14,751,032..14,751,032 (Dorn et al., 1993, Greenberg et al., 2004)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Transposable element inserted 5' of the transcription start site. (Granok et al., 2001)
Caused by insertion	P{hsneo.ry⁺}Trl⁶² (Dorn et al., 1993, Greenberg et al., 2004)
Cytology
Phenotypic Data
Phenotypic Class
	enhancer of variegation (Farkas et al., 1994) enhancer of variegation, with In(1)w^m4 (Sass and Henikoff, 1998) enhancer of variegation, with In(2R)bw^VDe2 (Sass and Henikoff, 1998) enhancer of variegation \| dominant, with In(1)w^m4h (Dorn et al., 1993) lethal \| larval stage \| recessive (Bhat et al., 1996, Farkas et al., 1994) lethal \| male (with Trl^13C) (Greenberg et al., 2004) lethal \| recessive (Katokhin et al., 2001, Granok et al., 2001) viable \| female \| reduced (with Trl^13C) (Greenberg et al., 2004) visible \| homeotic (Gerasimova and Corces, 1998)
Phenotype Manifest In

Detailed Description
	Statement Reference Trl^13C/Trl⁶² females have a similar viability to homozygotes, males show a significantly reduced viability. (Greenberg et al., 2004) Most homozygotes die as third instar larvae. (Granok et al., 2001) Lethality occurs at metamorphosis. (Katokhin et al., 2001) brm² shows little if any interaction in double heterozygous combination with Trl⁶²; less than 3% of flies have held-out wings. (Vazquez et al., 1999) Enhances the position effect variegation at the w locus caused by In(1)w^m4 and enhances the position effect variegation at the bw locus caused by In(2R)bw^VDe2. Does not enhance position effect variegation at the bw locus caused by Byron or the position effect variegation of w seen in the P{wA^R}41A insertion. (Sass and Henikoff, 1998) Transheterozygotes with Trl^13C are even less viable than Trl^13C homozygotes. (Bhat et al., 1996) Moderate enhancer of position effect variegation. Heterozygotes with Trl^13C eclose late, are poorly viable and exhibit the same phenotypes as Trl^13C homozygotes. (Farkas et al., 1994) Enhances variegation of In(1)w^m4h. (Dorn et al., 1993)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement Reference Trl⁶²/Trl^13C has lethal \| male phenotype, enhanceable by msl-1¹/msl-1[+] (Greenberg et al., 2004) Trl⁶²/Trl^13C has lethal \| male phenotype, enhanceable by msl-1¹/msl-1[+]/msl-2[+]/msl-2¹/mle¹/mle[+] (Greenberg et al., 2004) Trl⁶²/Trl^13C has lethal \| male phenotype, enhanceable by msl-2[+]/msl-2¹ (Greenberg et al., 2004) Trl⁶² has visible \| homeotic phenotype, enhanceable by mod(mdg4)^T16 (Gerasimova and Corces, 1998) Trl⁶² has visible \| homeotic phenotype, enhanceable by mod(mdg4)^ul (Gerasimova and Corces, 1998)
NOT Enhanced by
Statement Reference Trl⁶²/Trl^13C has viable \| female \| reduced phenotype, non-enhanceable by msl-1¹/msl-1[+] (Greenberg et al., 2004) Trl⁶²/Trl^13C has viable \| female \| reduced phenotype, non-enhanceable by msl-1¹/msl-1[+]/msl-2[+]/msl-2¹/mle¹/mle[+] (Greenberg et al., 2004)
Suppressed by
Statement Reference Trl⁶²/Trl^13C has lethal \| male phenotype, suppressible \| partially by msl-2^Hsp83.PK (Greenberg et al., 2004)
NOT suppressed by
Statement Reference Trl⁶²/Trl^13C has viable \| female \| reduced phenotype, non-suppressible by mle¹/mle[+] (Greenberg et al., 2004) Trl⁶²/Trl^13C has viable \| female \| reduced phenotype, non-suppressible by msl-1¹/msl-1[+] (Greenberg et al., 2004)
Enhancer of
Statement Reference Trl⁶² is an enhancer of body color defective phenotype of mod(mdg4)^ul, y² (Gerasimova and Corces, 1998) Trl⁶² is an enhancer of eye color defective phenotype of w^Fab-7.5F24 (Cavalli, 1999)
Suppressor of
Statement Reference Trl⁶² is a suppressor of lethal phenotype of Ras85D^V12.sev, sev^{S11.T:Hsap\MYC} (Maixner et al., 1998)
Other
Statement Reference Ras85D^V12.sev, Trl⁶², sev^{S11.T:Hsap\MYC} has viable phenotype (Maixner et al., 1998, Maixner et al., 1998) Trl⁶²/Trl^13C, msl-2^Hsp83.PK has lethal \| partially phenotype (Greenberg et al., 2004, Greenberg et al., 2004)
Phenotype Manifest In
Enhancer of
Statement Reference Trl[+]/Trl⁶² is an enhancer of sex comb \| ectopic & mesothoracic leg phenotype of Pc³ (Strutt et al., 1997) Trl[+]/Trl⁶² is an enhancer of sex comb \| ectopic & mesothoracic leg phenotype of Pc¹⁵ (Strutt et al., 1997) Trl⁶² is an enhancer of adult cuticle \| male phenotype of mod(mdg4)^ul, y² (Gerasimova and Corces, 1998) Trl⁶² is an enhancer of mesothoracic leg phenotype of Pc³ (Strutt et al., 1997) Trl⁶² is an enhancer of mesothoracic leg phenotype of Pc¹⁵ (Strutt et al., 1997) Trl⁶² is an enhancer of pigment cell phenotype of w^Fab-7.5F24 (Cavalli, 1999)
Suppressor of
Statement Reference Trl⁶² is a suppressor \| partially of eye phenotype of CycE^JP (Brumby et al., 2002)
Additional Comments
Genetic Interactions
Statement Reference Mutant weakly suppresses the CycE^JP rough eye phenotype. (Brumby et al., 2002) Mutant heterozygotes show a weak enhancement of the eye phenotype see in P{UAS-lacZ.Abd-B.5F24} leading to lighter eyes. (Cavalli, 1999) The frequency of homeotic transformations seen in heterozygotes is increased in double heterozygous combination with mod(mdg4)^ul or mod(mdg4)^T16. Dominantly enhances the effect of mod(mdg4)^ul on the y² phenotype. (Gerasimova and Corces, 1998) The formation of extra sex combs on the second and third legs in Pc⁴/+ flies is not enhanced by Trl⁶². (Kehle et al., 1998) The lethality caused by sev^{S11.T:Hsap\MYC} in combination with Ras85D^V12.sev is suppressed by Trl⁶². (Maixner et al., 1998)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Not rescued by	Trl⁶²/Trl^13C is not rescued by Trl^hs.PG (Granok et al., 2001) Trl⁶²/Trl^R67 is not rescued by Trl^hs.PG (Granok et al., 2001) Trl⁶² is not rescued by Trl^hs.PG (Granok et al., 2001) Trl^R85/Trl⁶² is not rescued by Trl^hs.PG (Granok et al., 2001)
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

Comments
	Attempts to make germ-line clones with this allele failed. (Liaw et al., 1995) Weak enhancer. High spontaneous background rate of E(var) mutations in these experiments allows for the possibility that the P insertion is not the cause of the E(var) phenotype. (Dorn et al., 1993)
External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 6 )
Reported As
Symbol Synonym	E(var)62 (Dorn et al., 1993) E(var)62¹ GAGA^Trl62 (Laney and Biggin, 1996) Trl62 (Katokhin et al., 2001) trl⁶² (Maixner et al., 1998) Trl⁶² (Vázquez et al., 2008)
Name Synonym
Secondary FlyBase IDs

References ( 19 )
Research paper	Vázquez et al., 2008, Genetics 180(2): 835--843 γTub23C Interacts Genetically With Brahma Chromatin-Remodeling Complexes in Drosophila melanogaster. [FBrf0206007] Greenberg et al., 2004, Genetics 166(1): 279--289 The Drosophila GAGA factor is required for dosage compensation in males and for the formation of the male-specific-lethal complex chromatin entry site at 12DE. [FBrf0174708] Erhardt et al., 2003, Dev. Genes Evol. 213(7): 336--344 Polycomb-group proteins are involved in silencing processes caused by a transgenic element from the murine imprinted H19/Igf2 region in Drosophila. [FBrf0160509] Brumby et al., 2002, EMBO J. 21(13): 3377--3389 Drosophila cyclin E interacts with components of the Brahma complex. [FBrf0151282] Granok et al., 2001, Genet. Res. (Camb.) 78(1): 13--21 A heat-shock-activated cDNA encoding GAGA factor rescues some lethal mutations in the Drosophila melanogaster Trithorax-like gene. [FBrf0138396] Katokhin et al., 2001, Genetika, Moscow 37(4): 467--474 [Molecular genetic analysis of Trithorax-like gene encoded transcriptional factor GAGA in Drosophila melanogaster]. [FBrf0136955] Espinas et al., 2000, EMBO Rep. 1(3): 253--259 The GAGA factor of Drosophila interacts with SAP18, a Sin3-associated polypeptide. [FBrf0133277] Vazquez et al., 1999, Development 126(4): 733--742 The trithorax group gene osa encodes an ARID-domain protein that genetically interacts with the brahma chromatin-remodeling factor to regulate transcription. [FBrf0107412] Gerasimova and Corces, 1998, Cell 92(4): 511--521 Polycomb and trithorax group proteins mediate the function of a chromatin insulator. [FBrf0100803] Kehle et al., 1998, Science 282(5395): 1897--1900 dMi-2, a hunchback-interacting protein that functions in polycomb repression. [FBrf0106056] Maixner et al., 1998, Dev. Genet. 23(4): 347--361 A screen for mutations that prevent lethality caused by expression of activated Sevenless and Ras1 in the Drosophila embryo. [FBrf0105879] Sass and Henikoff, 1998, Genetics 148(2): 733--741 Comparative analysis of position-effect variegation mutations in Drosophila melanogaster delineates the targets of modifiers. [FBrf0102004] Strutt et al., 1997, EMBO J. 16(12): 3621--3632 Co-localization of Polycomb protein and GAGA factor on regulatory elements responsible for the maintenance of homeotic gene expression. [FBrf0096385] Bhat et al., 1996, Development 122(4): 1113--1124 The GAGA factor is required in the early Drosophila embryo not only for transcriptional regulation but also for nuclear division. [FBrf0086911] Laney and Biggin, 1996, Development 122(7): 2303--2311 Redundant control of Ultrabithorax by zeste involves functional levels of zeste protein binding at the Ultrabithorax promoter. [FBrf0088370] Liaw et al., 1995, Genes Dev. 9(24): 3163--3176 The torso response element binds GAGA and NTF-1/Elf-1, and regulates tailless by relief of repression. [FBrf0085458] Farkas et al., 1994, Nature 371(6500): 806--808 The Trithorax-like gene encodes the Drosophila GAGA factor. [FBrf0076985] Dorn et al., 1993, Genetics 133(2): 279--290 P transposon-induced dominant enhancer mutations of position-effect variegation in Drosophila melanogaster. [FBrf0058558]
Review	Cavalli, 1999, Science 286(5441): 955--958 Epigenetic inheritance of active chromatin after removal of the main transactivator. [FBrf0112149]

Allele Dmel\Trl62

Allele Dmel\Trl⁶²