FlyBase Allele Report: Dmel\N[264-39]

General Information

Symbol

Dmel\N^264-39

Species

D. melanogaster

Name

FlyBase ID

FBal0029934

Feature type

allele

Associated gene

Dmel\N

Associated Insertion(s)

Carried in Construct

Also Known As

Notch^264-39, N^264.39, Df(1)N^264-39

Key Links

Allele class

amorphic allele - genetic evidence

loss of function allele

Mutagen

Nature of the Allele

Allele class

amorphic allele - genetic evidence

(Mari-Beffa et al., 1991)

loss of function allele

(Hagedorn et al., 2006, de Celis et al., 1991, Lehmann et al., 1983)

Mutagen

spontaneous

X ray

(Slizynska, 1938)

Mutations Mapped to the Genome

Type

Location

Additional Notes

References

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Progenitor genotype

Cytology

Nature of the lesion

Statement

Reference

Deficient for band 3C7.

(Slizynska, 1938)

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

corpus cardiacum primordium

(Park et al., 2011)

cuticle & macrochaeta | somatic clone

(Dietrich and Campos-Ortega, 1984)

embryonic/larval corpus cardiacum

(Park et al., 2011)

embryonic epidermis

(Lehmann et al., 1983)

embryonic hindgut

(Takashima et al., 2002)

eye

(Richardson and Pichaud, 2010)

eye | somatic clone

(Dietrich and Campos-Ortega, 1984)

eye photoreceptor cell | somatic clone

(Dietrich and Campos-Ortega, 1984)

female germline stem cell

(Song et al., 2007)

germarium cap cell

(Xin et al., 2013, Song et al., 2007)

glioblast

(Jacobs et al., 1989)

hub cell | first instar larval stage

(Okegbe and DiNardo, 2011)

interommatidial bristle | somatic clone

(Dietrich and Campos-Ortega, 1984)

intestinal stem cell of posterior adult midgut epithelium | somatic clone

(Lu and Li, 2015)

macrochaeta | somatic clone

(Dietrich and Campos-Ortega, 1984)

ommatidium | somatic clone

(Dietrich and Campos-Ortega, 1984)

pigment cell | somatic clone

(Dietrich and Campos-Ortega, 1984)

posterior wing margin

(Hagedorn et al., 2006)

wing

(Haberman et al., 2010, Baker and Rubin, 1992)

wing margin

(Xie et al., 2014, Dalton et al., 2011, MacDougall et al., 2004, Sakata et al., 2004)

wing margin bristle

(Dalton et al., 2011)

wing vein

(Dalton et al., 2011)

Detailed Description

Statement

Reference

Heterozygous N^264-39 flies show mild wing margin notching in the distal region in about half of the wings.

(Xie et al., 2014)

Females heterozygous for N^264-39, compared with wild-type, show a reduction in germarium cap cells 7 days after eclosion .

(Xin et al., 2013)

Heterozygous N^264-39 wings have a mild notched wing margin, bristle loss, and slight vein thickening near the wing blades.

(Dalton et al., 2011)

Stage 13 mutant male embryos have a normal number of somatic gonadal precursor cells. However, the gonads of mutant first instar male larvae lack hub cells.

(Okegbe and DiNardo, 2011)

The number of corpora cardiaca (CC) cells is increased in mutant stage 17 embryos compared to wild type. The CC cell lineage does not develop precociously in the mutant embryos, but multiple gl-positive CC precursors are detectable in the mutant embryos at early stage 11 (in contrast to the pair of single precursor cells seen in wild-type embryos at this stage).

(Park et al., 2011)

Heterozygotes have a notched wing phenotype.

(Haberman et al., 2010)

Zygotic N^264-39 mutant embryos display a massive expansion of neuronal cells in the entire ectodermal tissue.

(Kawahashi and Hayashi, 2010)

Flies heterozygous for N^264-39 have smaller eyes than wild-type flies, but their head capsule appears of normal size.

(Richardson and Pichaud, 2010)

The number of cap cells and germline stem cells in the ovaries of 2 day old heterozygous females is significantly reduced compared to wild type.

(Song et al., 2007)

Approximately 36.36% of heterozygous N^264-39 flies exhibit a haploinsufficient phenotype of wing notching at the posterior margins.

(Hagedorn et al., 2006)

Heterozygotes show notching of the wing margin at the anterior-posterior border.

(MacDougall et al., 2004)

Heterozygotes have a nicked wing margin phenotype.

(Sakata et al., 2004)

Boundary cells of the hindgut fail to develop in mutant embryos.

(Takashima et al., 2002)

The wing-notching phenotype of N^264-39 heterozygotes is more severe in flies which also carry one or two copies of Egfr^E3 or Egfr^E1.

(Baker and Rubin, 1992)

Cause a complex pleiotropic syndrome including perturbations of the chaetae pattern.

(de Celis et al., 1991)

Mutation causes overproduction of glioblasts.

(Jacobs et al., 1989)

Homozygous clones in the eye have a severely disturbed ommatidial pattern, visible as a scar in the eye surface. Ommatidia are larger than wild-type and interommatidial bristles are missing. Each ommatidium contains more retinula cells and fewer pigment cells than wild-type. Each ommatidium contains more receptor cells than normal, and may contain up to 13 receptor cells. Homozygous clones in the cuticle lack all bristles. Homozygous mutant clones in the eye are smaller and less frequent than the corresponding control clones.

(Dietrich and Campos-Ortega, 1984)

Extreme embryonic neurogenic phenotype.

(Lehmann et al., 1983)

Lethal in combination with N^l1N-ts1 or N^Ax-tsl at 29^oC. Homozygous lethal at 29^oC.

(Shellenbarger and Mohler, 1975)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

N^264-39 has visible | dominant phenotype, enhanceable by Scer\GAL4^bbg-C96/Taf9^{dsRNA.UAS.cUa}

(Xie et al., 2014)

N^264-39 has visible | dominant phenotype, enhanceable by Taf9¹⁹⁰/e(y)1[+]

(Xie et al., 2014)

N^264-39 has visible | dominant phenotype, enhanceable by hkl[+]/Acn¹

(Haberman et al., 2010)

N^264-39 has visible | dominant phenotype, enhanceable by Acn²⁷/Acn[+]

(Haberman et al., 2010)

N^264-39 has visible | dominant phenotype, enhanceable by Pi3K68D^{KD.UAS.Tag:MYC}/Scer\GAL4^69B

(MacDougall et al., 2004)

N^264-39 has visible | dominant phenotype, enhanceable by Df(3L)vin3/+

(MacDougall et al., 2004)

NOT Enhanced by

Statement

Reference

N^264-39 has visible | dominant phenotype, non-enhanceable by Pi3K68D^UAS.Tag:MYC/Scer\GAL4^69B

(MacDougall et al., 2004)

N^264-39 has visible | dominant phenotype, non-enhanceable by Df(3R)Pi3K92E^A/+

(MacDougall et al., 2004)

Suppressed by

Statement

Reference

N^264-39 has abnormal size | dominant phenotype, suppressible by crb^11A22/crb[+]

(Richardson and Pichaud, 2010)

N^264-39 has visible | dominant phenotype, suppressible by Scer\GAL4[-]/Nedd4^T119FS

(Sakata et al., 2004)

NOT suppressed by

Statement

Reference

N^264-39 has visible | dominant phenotype, non-suppressible by Df(3R)Pi3K92E^A/+

(MacDougall et al., 2004)

Enhancer of

Statement

Reference

N^264-39 is an enhancer of visible phenotype of Scer\GAL4^ey.PH, brm^{K804R.UAS.Tag:HA}

(Armstrong et al., 2005)

N[+]/N^264-39 is an enhancer of abnormal planar polarity phenotype of fz^hs.sev

(Fanto and Mlodzik, 1999)

N[+]/N^264-39 is an enhancer of abnormal planar polarity phenotype of dsh^hs.sev.B

(Fanto and Mlodzik, 1999)

NOT Enhancer of

Statement

Reference

N^264-39 is a non-enhancer of lethal phenotype of Nf-YA^UAS.Tag:HA, Scer\GAL4^ey.PH

(Yoshioka et al., 2007)

N[+]/N^264-39 is a non-enhancer of visible phenotype of CycE^JP

(Brumby et al., 2004)

Suppressor of

Statement

Reference

N[+]/N^264-39 is a suppressor of abnormal phototaxis phenotype of Hsap\CHMP2B^{Intron5.UAS.N.Tag:FLAG}, Scer\GAL4^GMR.PF

(Cheruiyot et al., 2014)

N[+]/N^264-39 is a suppressor of hyperplasia | somatic clone | cell non-autonomous phenotype of Vps25^N55

(Herz et al., 2009)

N[+]/N^264-39 is a suppressor of visible | somatic clone | heat sensitive phenotype of Uba1^D6

(Lee et al., 2008)

NOT Suppressor of

Statement

Reference

N^264-39 is a non-suppressor of lethal phenotype of Nf-YA^UAS.Tag:HA, Scer\GAL4^ey.PH

(Yoshioka et al., 2007)

N[+]/N^264-39 is a non-suppressor of visible phenotype of CycE^JP

(Brumby et al., 2004)

Phenotype Manifest In

Enhanced by

Statement

Reference

N^264-39 has wing margin phenotype, enhanceable by Scer\GAL4^bbg-C96/Taf9^{dsRNA.UAS.cUa}

(Xie et al., 2014)

N^264-39 has wing margin phenotype, enhanceable by Taf9¹⁹⁰/e(y)1[+]

(Xie et al., 2014)

N^264-39 has germarium cap cell phenotype, enhanceable by enok¹/enok[+]

(Xin et al., 2013)

N^264-39 has wing margin phenotype, enhanceable by Ndfip^KK108770/Scer\GAL4^Bx-MS1096

(Dalton et al., 2011)

N^264-39 has wing vein phenotype, enhanceable by Ndfip^KK108770/Scer\GAL4^Bx-MS1096

(Dalton et al., 2011)

N^264-39 has wing phenotype, enhanceable by hkl[+]/Acn¹

(Haberman et al., 2010)

N^264-39 has wing phenotype, enhanceable by Acn²⁷/Acn[+]

(Haberman et al., 2010)

N^264-39 has posterior wing margin phenotype, enhanceable by aux^I670K/aux[+]

(Hagedorn et al., 2006)

N^264-39 has wing margin phenotype, enhanceable by Pi3K68D^{KD.UAS.Tag:MYC}/Scer\GAL4^69B

(MacDougall et al., 2004)

N^264-39 has wing margin phenotype, enhanceable by Df(3L)vin3/+

(MacDougall et al., 2004)

NOT Enhanced by

Statement

Reference

N^264-39 has wing margin phenotype, non-enhanceable by Pi3K68D^UAS.Tag:MYC/Scer\GAL4^69B

(MacDougall et al., 2004)

N^264-39 has wing margin phenotype, non-enhanceable by Df(3R)Pi3K92E^A/+

(MacDougall et al., 2004)

Suppressed by

Statement

Reference

N^264-39 has wing margin phenotype, suppressible by Ndfip^UAS.Tag:HA/Scer\GAL4^Bx-MS1096

(Dalton et al., 2011)

N^264-39 has wing margin bristle phenotype, suppressible by Ndfip^UAS.Tag:HA/Scer\GAL4^Bx-MS1096

(Dalton et al., 2011)

N^264-39 has wing vein phenotype, suppressible by Ndfip^UAS.Tag:HA/Scer\GAL4^Bx-MS1096

(Dalton et al., 2011)

N^264-39 has eye phenotype, suppressible by crb^11A22/crb[+]

(Richardson and Pichaud, 2010)

N^264-39 has wing margin phenotype, suppressible by Scer\GAL4[-]/Nedd4^T119FS

(Sakata et al., 2004)

NOT suppressed by

Statement

Reference

N^264-39 has intestinal stem cell of posterior adult midgut epithelium | somatic clone phenotype, non-suppressible by Scer\GAL4^NP5130/Scer\GAL80^ts.αTub84B/E(spl)mβ-HLH^UAS.cdCa

(Lu and Li, 2015)

N^264-39 has intestinal stem cell of posterior adult midgut epithelium | somatic clone phenotype, non-suppressible by Scer\GAL4^NP5130/Scer\GAL80^ts.αTub84B/E(spl)mα-BFM^UAS.cAa

(Lu and Li, 2015)

N^264-39 has wing margin phenotype, non-suppressible by Df(3R)Pi3K92E^A/+

(MacDougall et al., 2004)

Enhancer of

Statement

Reference

N[+]/N^264-39 is an enhancer of wing margin phenotype of N¹

(Xie et al., 2014)

N^264-39 is an enhancer of eye phenotype of Scer\GAL4^ey.PH, brm^{K804R.UAS.Tag:HA}

(Armstrong et al., 2005)

N[+]/N^264-39 is an enhancer of photoreceptor cell R4 phenotype of fz^hs.sev

(Fanto and Mlodzik, 1999)

N[+]/N^264-39 is an enhancer of ommatidium phenotype of fz^hs.sev

(Fanto and Mlodzik, 1999)

N[+]/N^264-39 is an enhancer of photoreceptor cell R4 phenotype of dsh^hs.sev.B

(Fanto and Mlodzik, 1999)

N[+]/N^264-39 is an enhancer of ommatidium phenotype of dsh^hs.sev.B

(Fanto and Mlodzik, 1999)

NOT Enhancer of

Statement

Reference

N[+]/N^264-39 is a non-enhancer of eye phenotype of CycE^JP

(Brumby et al., 2004)

Suppressor of

Statement

Reference

N[+]/N^264-39 is a suppressor of eye phenotype of Hsap\CHMP2B^{Intron5.UAS.N.Tag:FLAG}, Scer\GAL4^ey.PH

(Cheruiyot et al., 2014)

N[+]/N^264-39 is a suppressor of ommatidium phenotype of Hsap\CHMP2B^{Intron5.UAS.N.Tag:FLAG}, Scer\GAL4^ey.PH

(Cheruiyot et al., 2014)

N[+]/N^264-39 is a suppressor of eye | somatic clone phenotype of Utx¹

(Herz et al., 2010)

N^264-39 is a suppressor of adult head | somatic clone phenotype of lsn^5F3-8

(Herz et al., 2009)

N[+]/N^264-39 is a suppressor of eye | somatic clone phenotype of Vps25^N55

(Herz et al., 2009)

N[+]/N^264-39 is a suppressor of adult head | somatic clone phenotype of Vps25^N55

(Herz et al., 2009)

N^264-39 is a suppressor of eye | somatic clone phenotype of lsn^5F3-8

(Herz et al., 2009)

N[+]/N^264-39 is a suppressor of eye | somatic clone | heat sensitive phenotype of Uba1^D6

(Lee et al., 2008)

NOT Suppressor of

Statement

Reference

N[+]/N^264-39 is a non-suppressor of eye | somatic clone phenotype of Vps36^L5212

(Herz et al., 2009)

N[+]/N^264-39 is a non-suppressor of eye phenotype of CycE^JP

(Brumby et al., 2004)

Additional Comments

Genetic Interactions

Statement

Reference

Combination of Scer\GAL4^bbg-C96>e(y)1^{dsRNA.Scer\UAS.cUa} and N^264-39/+ results in a synergistic enhancement of the notched wing phenotype in terms of both penetrance and expressivity.

Although e(y)1¹⁹⁰ heterozygous flies do not show any visible wing defects, e(y)1¹⁹⁰/+ greatly enhances the wing margin notching phenotype of N^264-39/+ in both expressivity and penetrance.

(Xie et al., 2014)

The germarium cap cell loss caused by N^264-39/+ is significantly enhanced by enok¹/+.

(Xin et al., 2013)

Scer\GAL4^Bx-MS1096-mediated expression of Ndfip^{Scer\UAS.T:Ivir\HA1} suppresses the N^264-39/+ wing phenotypes.

Scer\GAL4^Bx-MS1096-mediated expression of Ndfip^KK108770 enhances the wing margin notching and slight vein thickening near the wing blade seen in N^264-39/+ wings.

(Dalton et al., 2011)

The severity of the notched wing phenotype seen in N^264-39/+ flies is enhanced by Acn²⁷/+ and by Acn¹/+.

(Haberman et al., 2010)

Heterozygosity for N^264-39 suppresses the over-representation phenotype of Utx¹ mosaics.

(Herz et al., 2010)

Heterozygous crb^11A22 suppresses the N^264-39/+ small eye phenotype. The suppression is achieved without altering the size of the facet lenses.

(Richardson and Pichaud, 2010)

Heterozygosity for N^264-39 suppresses the overgrowth of the adult eye and head that occurs in animals with somatic clones of Vps25^N55 generated in the eye-antennal disc.

Heterozygosity for N^264-39 does not suppresses the rough-eye phenotype that occurs in animals with somatic clones of Vps36^L5212 generated in the eye-antennal disc.

Heterozygosity for N^264-39 suppresses the overgrowth of the adult eye and head that occurs in animals with somatic clones of lsn^5F3-8 generated in the eye-antennal disc.

(Herz et al., 2009)

The eye overgrowth phenotype seen in Uba1^D6 mosaics raised at 29[o]C is dominantly suppressed by N^264-39.

(Lee et al., 2008)

A heterozygous aux^I670K background increases the severity of both the penetrance and the phenotype of N^264-39 heterozygous mutants.

(Hagedorn et al., 2006)

The wing notching phenotype seen in N^264-39/+ flies is enhanced by expression of Pi3K68D^{KD.Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^69B, but is not enhanced by expression of Pi3K68D^{Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^69B.

Df(3L)vin3/+ enhances the wing notching phenotype seen in N^264-39/+ flies.

Df(3R)Pi3K92E^A/+ has no effect on the wing notching phenotype seen in N^264-39/+ flies.

(MacDougall et al., 2004)

The nicked wing phenotype seen in N^264-39/+ flies is suppressed by Nedd4^T119FS/+.

(Sakata et al., 2004)

Dominantly enhances the eye-polarity phenotype of fz^hs.sev; most fz^hs.sev ommatidia show R3/R3-type symmetry in a N^264-39 heterozygous background. Dominantly enhances the eye-polarity phenotype of dsh^hs.sev.B.

(Fanto and Mlodzik, 1999)

Xenogenetic Interactions

Statement

Reference

A N^264-39 heterozygous background suppresses the eye deformities, eye curvature, patterning of the photoreceptor nuclei later, and phototactic behavior seen in flies expressing Hsap\CHMP2B^{Intron5.Scer\UAS.N.T:Zzzz\FLAG} under the control of Scer\GAL4^ey.PH.

(Cheruiyot et al., 2014)

Complementation and Rescue Data

Fails to complement

(Slizynska, 1938)

(Slizynska, 1938)

(Slizynska, 1938)

(Slizynska, 1938)

Rescued by

N^264-39 is rescued by N^GV.3.hs

(Okegbe and DiNardo, 2011)

Partially rescued by

N^264-39 is partially rescued by Scer\GAL4^prd.RG1/N^UAS.GFP

(Kawahashi and Hayashi, 2010)

Comments

Expression of N^{3.hs.T:Scer\GAL4,T:Hsim\VP16} using three 40 minute heat shocks starting at 8-9 hours after egg laying does not rescue hub cell number in the gonads of male N^264-39 larvae. However, expression of N^{3.hs.T:Scer\GAL4,T:Hsim\VP16} using three 40 minute heat shocks starting at 5-6 hours after egg laying results in significant rescue of hub cell number in the gonads of male N^264-39 larvae, with almost half of the animals reaching the wild-type range of hub cells.

(Okegbe and DiNardo, 2011)

Expression of N^{Scer\UAS.T:Avic\GFP} under the control of Scer\GAL4^prd.RG1 greatly reduces the amount of neuronal cells in N^264-39 mutants.

(Kawahashi and Hayashi, 2010)

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

730

w^ch2 N^264-39/FM4, B⁺

Kyoto

106049

w^ch2 N^264-39/FM4, B⁺

Notes on Origin

Discoverer

Slizynska, 1937.

Arose on: w^ch.

Comments

Originally reported as a deficiency of band 3C7 (FBrf0004288). Later reexamination of chromosomes of males from lines carrying w^ch and marked N^264-39 revealed the presence of 3C7 (Welshons)

Cytological examination reveals that this mutant chromosome is no longer cytologically deficient.

(Welshons, 1965)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (7)

Reported As

Symbol Synonym

264-39

(Slizynska, 1938)

Df(1)N-264-39

Df(1)N^264-39

(Welshons, 1974, Welshons, 1965)

N^264-39

(Zhao et al., 2022, Lu and Li, 2015, Cheruiyot et al., 2014, Xie et al., 2014, Xin et al., 2013, Dalton et al., 2011, Knox et al., 2011, Park et al., 2011, Haberman et al., 2010, Herz et al., 2010, Kawahashi and Hayashi, 2010, Richardson and Pichaud, 2010, Herz et al., 2009, Kandachar et al., 2008, Song et al., 2007, MacDougall et al., 2004)

N^264.39

(Okegbe and DiNardo, 2011, Villa-Cuesta et al., 2003, de Celis et al., 1991, Mari-Beffa et al., 1991)

Notch^264-39

(Shi et al., 2021, Ren et al., 2015, Bonn et al., 2013, Lee et al., 2008, Yoshioka et al., 2007)

Name Synonyms

Deficiency(1)Notch

Notch264-39

(Xin et al., 2013)

Secondary FlyBase IDs

FBab0000541

References (45)

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