FB2020_06, released Dec 22, 2020

Allele: Dmel\Pka-C1B10

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General Information
Symbol
Dmel\Pka-C1B10
Species
D. melanogaster
Name
FlyBase ID
FBal0030038
Feature type
allele
Associated gene
Dmel\Pka-C1
Associated Insertion(s)
Carried in Construct
Also Known As
DC0B10, DCOB10
Genomic Maps
Allele class

hypomorphic allele - genetic evidence

Mutagen
Nature of the Allele
Allele class

hypomorphic allele - genetic evidence

(Davis et al., 1993, Skoulakis et al., 1993)
Mutagen
ethyl methanesulfonate
(Davis et al., 1993, Skoulakis et al., 1993)
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
point mutation
2L:9,696,911..9,696,911 [-]
Nucleotide change:

A9696911T

Reported nucleotide change:

A2779T

Amino acid change:

N219I | Pka-C1-PB; N219I | Pka-C1-PC; N219I | Pka-C1-PD

Reported amino acid change:

N219I

(Lane and Kalderon, 1993)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
       
      Progenitor genotype
      Cytology
      Nature of the lesion
      Statement
      Reference

      Missense mutation within the Pka-C1 coding region.

      (Davis et al., 1993)

      Amino acid replacement: N219I.

      (Lane and Kalderon, 1993, Skoulakis et al., 1993)
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Pka-C1B10 heterozygotes do not show any obvious olfactory learning or memory defects upon an olfactory aversive conditioning assay, as compared to controls.

      (Lee et al., 2018)

      Heterozygotes have significantly lower basal fluid secretion rates from the Malpighian tubules compared to wild type.

      (Efetova et al., 2013)

      Pka-C1B10/Pka-C1Tw2 mutant flies show an inability to find a preferred temperature, with temperature preference across a wide gradient.

      (Hong et al., 2008)

      Border follicle cell migration is normal in Pka-C1B10/Pka-C1Tw2 females.

      (Lane and Kalderon, 1995)

      Heterozygotes exhibit rhythmic locomotor activity in constant darkness and the average circadian rhythm is slightly shorter than wild type. Heterozygotes had ostensibly normal behaviour.

      (Levine et al., 1994)

      Transheterozygotes for weak Pka-C1 alleles are lethal at 18oC. Hemizygotes are larval lethal with some adult escapers.

      (Lane and Kalderon, 1993)

      Heterozygotes exhibit an olfactory learning deficit. Pka-C1MB581/Pka-C1B10 transheterozygotes are viable and exhibit a dramatic olfactory learning and memory deficit.

      (Skoulakis et al., 1993)

      hypomorphic

      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Other
      Statement
      Reference

      Pka-C1[+]/Pka-C1B10, mengMI03008 has memory defective | conditional phenotype

      (Lee et al., 2018)

      Pka-C1[+]/Pka-C1B10, rut1 has thermotaxis defective | dominant phenotype

      (Hong et al., 2008)

      Pka-C1[+]/Pka-C1B10, amnchpd has thermotaxis defective | dominant phenotype

      (Hong et al., 2008)
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      mengMI03008, Pka-C1B10 double heterozygotes show a significant decrease in 24h olfactory memory, but not in learning, upon an olfactory aversive conditioning assay, as compared to controls; however, there are no effects in residual olfactory memory upon cold-shock, as compared to controls.

      (Lee et al., 2018)

      Double heterozygotes for amnchpd and Pka-C1B10 show an inability to find a preferred temperature, with temperature preference spread across a wide gradient.

      Double heterozygotes for rut1 and Pka-C1B10 show an inability to find a preferred temperature, with temperature preference spread across a wide gradient.

      (Hong et al., 2008)
      Xenogenetic Interactions
      Statement
      Reference

      Animals expressing Zzzz\bPACScer\UAS.cSa under the control of Scer\GAL4c42 in a Pka-C1B10/+ background show significant stimulation of fluid secretion from the Malpighian tubules in response to low light activation.

      (Efetova et al., 2013)
      Complementation and Rescue Data
      Rescued by

      Pka-C1B10 is rescued by Pka-C1+mTgDC0

      (Lane and Kalderon, 1993)
      Comments

      One copy of Pka-C1+mTgDC0 provides full rescue of hemizygotes.

      (Lane and Kalderon, 1993)
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer

      D. Kalderon.

      Comments
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      References (15)