FlyBase Allele Report: Dmel\Pka-C1[MB581]

General Information

Symbol

Dmel\Pka-C1^MB581

Species

D. melanogaster

Name

FlyBase ID

FBal0030039

Feature type

allele

Associated gene

Dmel\Pka-C1

Associated Insertion(s)

P{lArB}Pka-C1^MB581

Carried in Construct

Also Known As

DCO⁵⁸¹, DC0⁵⁸¹

Key Links

Genomic Maps

JBrowse

Allele class

hypomorphic allele - genetic evidence

Mutagen

P-element activity

Nature of the Allele

Allele class

hypomorphic allele - genetic evidence

(Li et al., 1995, Davis et al., 1993, Skoulakis et al., 1993)

Mutagen

P-element activity

(Lane and Kalderon, 1995, Davis et al., 1993, Skoulakis et al., 1993)

Progenitor genotype

Associated Insertion(s)

P{lArB}Pka-C1^MB581

Cytology

Description

P-element insertion just upstream of the transcription start site.

(Lane and Kalderon, 1995)

P-element insert into exon 1, 71bp downstream from the transcriptional start site of Pka-C1.

(Davis et al., 1993)

P{lArB} insertion in the first exon.

(Skoulakis et al., 1993)

Allele components

Component

Use(s)

Inserted element

P{lArB}

enhancer trap

Encoded product / tool

lacZ

reporter enzyme

Tagged with

Tag:NLS(P)

nuclear protein localization tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

2L:9,699,217..9,699,217 [-]

(Hong et al., 2008, Lane and Kalderon, 1995, Davis et al., 1993, Skoulakis et al., 1993)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal behavior

(Skoulakis et al., 1993)

abnormal circadian rhythm

(Majercak et al., 1997)

abnormal thermotaxis (with Pka-C1^X4)

(Hong et al., 2008)

lethal | recessive

(Levine et al., 1994, Davis et al., 1993, Skoulakis et al., 1993)

Phenotype Manifest In

Malpighian tubule

(Efetova et al., 2013)

Detailed Description

Statement

Reference

Heterozygotes have significantly lower basal fluid secretion rates from the Malpighian tubules compared to wild type.

(Efetova et al., 2013)

Pka-C1^X4/Pka-C1^MB581 mutant flies show an inability to find a preferred temperature, with temperature preference spread across a wide gradient.

(Hong et al., 2008)

Imaginal pattern duplications.

(Li et al., 1995)

Heterozygotes exhibit rhythmic locomotor activity in constant darkness and the average circadian rhythm is slightly shorter than wild type. Heterozygotes had ostensibly normal behaviour.

(Levine et al., 1994)

Initial learning is sightly reduced.

(Davis et al., 1993)

Homozygous lethality occurs at embryonic and early larval stages. Heterozygotes exhibit a decrease in olfactory learning. Pka-C1^MB581/Pka-C1^B10 transheterozygotes are viable and exhibit a dramatic olfactory learning and memory deficit.

(Skoulakis et al., 1993)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Partially rescued by

Pka-C1^X4/Pka-C1^MB581 is partially rescued by Scer\GAL4^Mef2.247/Pka-C1^UAS.cKa

(Hong et al., 2008)

Pka-C1^X4/Pka-C1^MB581 is partially rescued by Scer\GAL4^c309/Pka-C1^UAS.cKa

(Hong et al., 2008)

Comments

Expression of Pka-C1^Scer\UAS.cKa under the control of either Scer\GAL4^Mef2.247 or Scer\GAL4^c309 almost completely rescues the temperature preference behavior defects seen in Pka-C1^MB581/Pka-C1^X4 mutant.

(Hong et al., 2008)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (7)

Reported As

Symbol Synonym

DC0⁵⁸¹

(Efetova et al., 2013, Levine et al., 1994, Skoulakis et al., 1993)

DC0^MB581

(Hong et al., 2008)

DCO⁵⁸¹

(Majercak et al., 1997, Li et al., 1995, Skoulakis et al., 1993)

DCO^MB581

(Hong et al., 2008)

MB581

(Hong et al., 2008)

Pka-C1⁵⁸¹

(Stanewsky, 2003)

Pka-C1^MB581

Name Synonyms

Secondary FlyBase IDs

FBal0032018

References (12)

Report Sections

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