Allele Dmel\Ras64B^Val14.hs

General Information
Symbol	Dmel\Ras64B^Val14.hs	Species	D. melanogaster
Name		FlyBase ID	FBal0030097
Feature type	allele	Associated gene	Dmel\Ras64B

Allele class
Mutagen	in vitro construct - regulatory fusion, in vitro construct - amino acid replacement
Recent Updates
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FB2013_03
FB2013_02
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Nature of the Allele
Allele class
Mutagen	in vitro construct - amino acid replacement (Lu et al., 1993) in vitro construct - regulatory fusion (Bishop and Corces, 1988, Lu et al., 1993)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype	Ras64B^Val14 (Bishop and Corces, 1988)
Nature of the lesion	Statement Reference Construct: Expression of a 4.6kb XhoI Ras64B^Val14 fragment is driven by an Hsp70 promoter located 83 nucleotides upstream of the Ras64B initiator codon. (Bishop and Corces, 1988)
Carried in construct	P{C20-42} (Lu et al., 1993, Bishop and Corces, 1988)
Cytology
Phenotypic Data
Phenotypic Class
	lethal \| embryonic stage \| maternal effect (Lu et al., 1993, Bishop and Corces, 1988) visible \| dominant (Bishop and Corces, 1988)
Phenotype Manifest In
	anterior sternopleural bristle \| maternal effect (Bishop and Corces, 1988) dorsal triple row \| maternal effect (Bishop and Corces, 1988) dorsal wing blade \| maternal effect (Bishop and Corces, 1988) embryonic head (Lu et al., 1993) humeral sclerite \| maternal effect (Bishop and Corces, 1988) lower humeral bristle \| maternal effect (Bishop and Corces, 1988) medial triple row \| maternal effect (Bishop and Corces, 1988) middle sternopleural bristle \| maternal effect (Bishop and Corces, 1988) ommatidium \| maternal effect (Bishop and Corces, 1988) posterior sternopleural bristle \| maternal effect (Bishop and Corces, 1988) upper humeral bristle \| maternal effect (Bishop and Corces, 1988) ventral sternopleural bristle \| maternal effect (Bishop and Corces, 1988) ventral triple row \| maternal effect (Bishop and Corces, 1988) ventral wing blade \| maternal effect (Bishop and Corces, 1988)
Detailed Description
	Statement Reference One copy of the P{Ras85D^V12.hs} construct causes failure of embryos to hatch. Unhatched embryos display head defects. (Lu et al., 1993) One copy of P{C20-42} causes low fertility, laying many eggs that failed to hatch and causes developmental defects in the wing and eye development. Bristles are of variable size and placement on the wing anterior margin and sternopleural bristles and humeral bristles are bent or forked. Protuding cuticle growth of the humeral region. An unfused bloated wing phenotype is seen after heat shock during the early third instar to mid-pupal stages. Heat shock throughout all stages of development resulted in some degree of lethality, heat shock around pupariation causes total lethality. Dorsal-to-ventral scars develop in the middle-to-posterior portion of the adult eye after heat shock 1 to 2 days before pupariation. Pharate adults display scars of unpigmented, irregular and fused ommatidia. (Bishop and Corces, 1988)
External Data
Linkouts
Interactions
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement Reference
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 3 )
Reported As
Symbol Synonym	hsp70-ras^Val14 (Bishop and Corces, 1988) Ras2^V12 (Lu et al., 1993) Ras64B^Val14.hs
Name Synonym
Secondary FlyBase IDs

References ( 3 )
Research paper	Brand and Perrimon, 1993, Development 118(2): 401--415 Targeted gene expression as a means of altering cell fates and generating dominant phenotypes. [FBrf0064375] Lu et al., 1993, Genes Dev. 7(4): 621--632 Control of cell fate determination by p21^ras/Ras1, an essential component of torso signaling in Drosophila. [FBrf0058534] Bishop and Corces, 1988, Genes Dev. 2: 567--577 Expression of an activated ras gene causes developmental abnormalities in transgenic Drosophila melanogaster. [FBrf0048117]

Allele Dmel\Ras64BVal14.hs

Allele Dmel\Ras64B^Val14.hs